Article
Chemistry, Applied
Lu Zhang, Xin Qi, Xin-tong Lu, Cheng-bi Cui, Xue-feng Gao
Summary: This study explored the effects of different radiation doses on the hypoglycemic effects of the Co-60-gamma irradiated ginseng adventitious root extract. The irradiation increased the saponin content and radical scavenging ability of the extract. The irradiated extract showed superior protection on glomerular mesangial cells and significantly reduced glucose levels in mice with type 1 diabetes, possibly through the activation of the Keap1/Nrf2/HO-1 pathway and attenuation of oxidative stress.
Article
Horticulture
Hong Chen, Xiangzhu Li, Yongjun Zheng, Mingming Liu, Kangyu Wang
Summary: Panax ginseng, an ancient and potent herb, contains ginsenosides which contribute to its medicinal effects. Wild and cultivated ginseng has insufficient saponin content to meet market demand, but ginseng adventitious root suspension culture can produce targeted ginsenosides. The length of culture time impacts plant growth, secondary metabolite accumulation, and gene expression. Extraction and analysis revealed increased total saponins, protopanaxadiol ginsenosides (specifically Rd and Rb1), and gene changes related to ginsenoside biosynthesis.
Article
Cell Biology
Xiaohao Xu, Guang Sun, Jianzeng Liu, Jingyuan Zhou, Jing Li, Zhuo Sun, Xiangzhu Li, Hong Chen, Daqing Zhao, Rui Jiang, Liwei Sun
Summary: The study found that the adventitious root protein mixture (ARP) could significantly reduce cell damage caused by UVA radiation by promoting Akt phosphorylation to reduce DNA damage, decrease apoptosis, and cell cycle arrest. Additionally, ARP could protect cells from the decrease in collagen-I expression induced by UVA radiation.
WOUND REPAIR AND REGENERATION
(2021)
Article
Plant Sciences
Yang Liu, Naifu Zhou, Chengrui Luo, Qi Zhang, Peng Sun, Jianmin Fu, Shuzhan Li, Ze Li
Summary: This study examined the effects of different plant growth regulators and concentrations on tissue culture of persimmon trees and found the optimal conditions for germination of young embryos, induction of leaf callus, differentiation of adventitious shoots, and rooting culture.
Article
Biotechnology & Applied Microbiology
Mei-Yu Jin, Yue-Jun Hao, Ke-Xin Zhang, Cheng-Ri Yin, Jun Jiang, Xuan-Chun Piao, Mei-Lan Lian
Summary: This study investigated the effects of various factors on the production of flavonoid- and polysaccharide-enriched Oplopanax elatus adventitious roots in a fed-batch culture system. The optimal conditions were determined, and it was found that the fed-batch culture approach is a promising method for mass production of O. elatus adventitious roots, with high flavonoid and polysaccharide yields.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Plant Sciences
Hye Rim Seong, Cuicui Wang, Muhammad Irfan, Young Eun Kim, Gooyoung Jung, Sung Kyeong Park, Tae Myoung Kim, Ehn-Kyoung Choi, Man Hee Rhee, Yun-Bae Kim
Summary: DK-MGAR101, an extract of mountain ginseng adventitious roots containing various ginsenosides, exerts anti-thrombotic activity and improves blood flow. It protects against endothelial cell injury and effectively suppresses platelet aggregation and thrombus formation.
JOURNAL OF GINSENG RESEARCH
(2022)
Article
Genetics & Heredity
Sitong Liu, Jing Zhao, Yutong Liu, Ning Li, Zhenhui Wang, Xinfeng Wang, Xiaodong Liu, Lili Jiang, Bao Liu, Xueqi Fu, Xiaomeng Li, Linfeng Li
Summary: Chinese ginseng is a highly valued traditional Chinese medicine with long-term cultured callus cells showing high chromosomal stability and virtually immortalized totipotency. These unique features may be related to the robust homeostasis of transcriptional expression of specific genes implicated in tissue totipotency and chromosomal stability. Further exploration of the molecular mechanisms underlying these properties may provide novel insights into the mechanistic understanding of somatic chromosomal stability and occurrence of CIN in human disease.
Article
Plant Sciences
Sitong Liu, Xinfeng Wang, Ning Ding, Yutong Liu, Ning Li, Yiqiao Ma, Jing Zhao, Zhenhui Wang, Xiaomeng Li, Xueqi Fu, Linfeng Li
Summary: Plants have the ability to regenerate from various types of cells, but somatic cell totipotency can be compromised by somaclonal variation, particularly karyotype alteration, during tissue culture. Chinese ginseng exhibits little decline in totipotency and remarkable chromosomal stability even after prolonged tissue cultures. However, it is uncertain whether chromosomal stability at the molecular level necessarily correlates with genetic stability at the nucleotide sequence level.
Article
Forestry
Yang Liu, Xiaoyu Lu, Hui Zhang, Shuzhan Li, Ze Li
Summary: This study investigated the effects of different plant-growth regulators and concentrations on the culture of persimmon stems, induction of leaf callus, differentiation of adventitious shoots, and rooting culture. The optimal formula for inducing axillary buds and leaf callus was identified, and an efficient regeneration system for shoots and rooting of persimmon was developed. These findings provide theoretical support for the rapid and excellent growth of persimmon, care of factory saplings, and molecular breeding.
Article
Green & Sustainable Science & Technology
Haibo Zhang, Di He, Xiaoling Li, Bicheng Dun, Di Wu, Guiyun Huang
Summary: This study used the popular large-flowered variety 'RED SUN' of Phalaenopsis aphrodite Rchb. F. to investigate the effects of pedicel taking time, site, plant growth regulators, and organic substances on the establishment of a regeneration system. The optimal conditions and formulas were determined, providing a scientific basis for rapid breeding of Phalaenopsis orchids.
Article
Biotechnology & Applied Microbiology
Junjie Li, Yuan Yuan, Wen Jiang, Xueli Quan, Songquan Wu, Welin Wu
Summary: The study found that ABA is involved in the synthesis of ginsenosides Rg(1) and Re in PGARs under cold stress. ABA treatment efficiently promoted the accumulation of ginsenosides Rg(1) and Re in PGARs.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Review
Plant Sciences
Fengjiao Xu, Anjali Kariyarath Valappil, Ramya Mathiyalagan, Thi Ngoc Anh Tran, Zelika Mega Ramadhania, Muhammad Awais, Deok Chun Yang
Summary: This paper reviews the in vitro culture of Panax ginseng and the accumulation of ginsenosides. In vitro culture has been used for the large-scale production of bioactive compounds in ginseng. The paper also summarizes the enhanced levels of activity and content of ginsenosides with elicitor treatment, as well as the prospects of new breeding tools in ginseng.
Article
Biotechnology & Applied Microbiology
Young-Hun Kim, Chan-Woo Park, Woo-Jong Hong, Ick Hyun Jo, Keun Ki Kim, Yu-Jin Kim
Summary: A simple and efficient transformation system for ginseng was developed using ginseng callus cultured in a liquid medium and MgSO4 pretreatment before Agrobacterium infection. The transformation efficiency was improved by treating the cells with MgSO4. The introduction of the visible reporter gene RUBY facilitated the identification of transformed cells.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Agricultural Engineering
Kai-Hsi Liu, Hung-Yin Lin, James L. Thomas, Yuan-Pin Shih, Jen-Tsung Chen, Mei-Hwa Lee
Summary: In this study, molecularly imprinted polymers were developed for the extraction of ginsenosides, with lanosterol as a template for imprinting magnetic lanosterol-imprinted polymers (MLIPs). The use of MLIP nanoparticles for the absorptivity of ginsenosides from calluses of ginseng was successful, resulting in the extraction of ginsenosides Rg(1) and Rb-1.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Liulong Li, Xiaona Dong, Mengting He, Mei Huang, Jian Cai, Qin Zhou, Yingxin Zhong, Dong Jiang, Xiao Wang
Summary: Waterlogging priming is a promising technique for enhancing plant waterlogging tolerance. This study investigated the mechanisms behind root resistance in wheat under waterlogging stress and found that priming improved gas exchange, aerenchyma formation, and gene expression in adventitious roots, ultimately reducing the severity of damage caused by waterlogging.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Hosakatte Niranjana Murthy, Guggalada Govardhana Yadav, Sathish Shekhappa Kadapatti, M. Sandhya
Summary: The objective of this study was to evaluate the phytochemicals and antioxidant potential of Garcinia indica resin. Acetone and methanol extracts contain high amounts of phenolics and flavonoids, while methanol extract has the highest alkaloid content. GC-MS analysis identified terpenoids as the major compounds in acetone extract. Both acetone and methanol extracts showed excellent antioxidant activity. This study suggests that G. indica resin can be used for medicinal applications.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, So Young Park
Summary: This article introduces the major groups of medicinal plants in the Rubia genus, such as Rubia cordifolia, Rubia tinctorum, and Rubia akane. These plants contain anthraquinones (AQs), particularly alizarin and purpurin, which have various pharmacological effects. AQs have been used as natural dyes and color additives in industries. Different techniques and approaches, including cell and organ cultures, have been used to produce biomass and secondary metabolites from Rubia species. Bioreactor cultures have also been emphasized as a method to grow cells for AQ production.
Review
Biotechnology & Applied Microbiology
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, So Young Park
Summary: Since ancient times, Morinda species, especially Morinda citrifolia, have been used for therapeutic purposes. Various natural substances with bioactivity, such as iridoids, anthraquinones, coumarins, flavonoids, lignans, phytosterols, and carotenoids, have been identified. Among these, anthraquinone derivatives are the most important and are utilized as natural coloring agents with medicinal functions. This article provides a summary of the biotechnological methods used for the bioproduction of anthraquinone derivatives using cell and organ cultures of Morinda species. The production of these chemicals in bioreactor cultures is also discussed.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Serisha Gangaram, Yougasphree Naidoo, Yaser Hassan Dewir, Moganavelli Singh, Johnson Lin, Hosakatte Niranjana Murthy
Summary: This study characterized the phytochemical compounds and antibacterial efficacies of Barleria albostellata, a relatively understudied shrub in South Africa. The crude leaf and stem extracts exhibited antibacterial activity against various pathogenic microorganisms, and analysis revealed the presence of bioactive compounds with potential medicinal value. These findings highlight the potential of B. albostellata as a source for novel antibacterial agents.
Article
Plant Sciences
Ji-Hye Kim, Jong-Eun Han, Hosakatte Niranjana Murthy, Ja-Young Kim, Mi-Jin Kim, Taek-Kyu Jeong, So-Young Park
Summary: Sageretia thea was cultivated with the aim of increasing the production of phenolic compounds in plant cell suspension cultures. Optimum callus was induced and methyl jasmonate was found to be a suitable elicitor for phenolic accumulation in cell suspensions. The use of balloon-type bioreactors resulted in the highest biomass yields after four weeks of cultivation.
Article
Plant Sciences
Oluwatosin Temilade Adu, Yougasphree Naidoo, Johnson Lin, Depika Dwarka, John Mellem, Hosakatte Niranjana Murthy, Yaser Hassan Dewir
Summary: This study examined the nutritional properties and cytotoxic effects of D. villosa. The extracts and nanoparticles of D. villosa showed cytotoxic activity against cancerous cell lines, particularly MCF-7 and A549.
Article
Biology
Dayanand Dalawai, Hosakatte Niranjana Murthy, Yaser Hassan Dewir, Joseph Kadanthottu Sebastian, Anish Nag
Summary: This study determined the phytochemical composition and bioactive chemical components of Andrographis macrobotrys using GC-MS, and evaluated the antioxidant potential of its extracts. The results showed that the stem extract of the plant has a higher concentration of phenolics and exhibits strong antioxidant activity. These findings highlight the importance of A. macrobotrys as a medicinal plant and a potential source of antioxidants.
Article
Horticulture
Hosakatte Niranjana Murthy, Guggalada Govardhana Yadav, Sathish Shekhappa Kadapatti, Akarsha H. Pote, Ramalinga Jagali, Vidya Yarashi, Yaser Hassan Dewir
Summary: The present study investigated the nutritional composition, phytochemical composition, and antioxidant activities of Rourea minor fruits. The ripened seeds and pulp contained various components, including fat, protein, carbohydrates, ash, and fiber. Both the seeds and pulp were also rich in microelements. The study also found that the extracts from the fruits exhibited strong antioxidant activity. The findings suggest that Rourea minor fruits could be a valuable source of nutrients, microelements, and antioxidants.
Article
Horticulture
Yaser Hassan Dewir, Muhammad M. Habib, AbdulAziz A. AlQarawi, Thobayet S. Alshahrani, Ahmed Ali Alaizari, Jahangir A. Malik, Mona S. Alwahibi, Hosakatte Niranjana Murthy
Summary: This study examined the effect of arbuscular mycorrhizal fungi (AMF) on the growth and development of tissue-cultured Philodendron bipinnatifidum plantlets during the acclimatization phase. The results showed that AMF-treated plants had better growth characteristics and root development compared to non-AMF plants. These findings highlight the importance of mycorrhization in promoting vegetative growth and acclimatization of micropropagated plants.
Review
Biotechnology & Applied Microbiology
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, So Young Park
Summary: In vitro plant cell and organ cultures are attractive alternatives for producing valuable specialized metabolites. Adventitious roots are often superior to cell suspension cultures for accumulating high levels of specialized metabolites. Different bioreactor systems have been investigated for cultivation of adventitious roots.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Plant Sciences
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Kee Yoeup Paek, So Young Park
Summary: Plant cells and organ cultures are viable alternatives for producing specialized metabolites. Elicitation is a strategy used to enhance the accumulation of these compounds, and nanoparticles have recently been used as unique elicitors. This review summarizes the applications and usage of nanoparticles as elicitors in plant cell and organ cultures.
PLANT GROWTH REGULATION
(2023)
Review
Plant Sciences
Hosakatte Niranjana Murthy, Kadanthottu Sebastian Joseph, Jong-Eun Hahn, Han-Sol Lee, Kee Yoeup Paek, So Young Park
Summary: This review provides an update on the use of somatic embryogenesis in the production of valuable secondary metabolites, discussing the factors influencing their generation, elicitation methods, and prospective applications.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2023)
Article
Agronomy
Myeong-Jin Lim, Hosakatte Niranjana Murthy, Hyun-Young Song, Su-Young Lee, So-Young Park
Summary: This study investigated the effects of red, blue, white, and red plus blue and red, blue, and green LEDs on the in vitro regeneration of Gerbera jamesonii cv. 'Shy Pink'. The results showed that red plus blue LEDs had the greatest impact on shoot regeneration, growth, and root regeneration. Red and blue LEDs affected different parts of the plant, with blue LEDs promoting higher photosynthetic pigment content. White LEDs had a strong effect on chlorophyll fluorescence.
Article
Business
V. Mamtha, H. N. Narasimha Murthy, V. Pujith Raj, Prashantha Tejas, C. S. Puneet, Achyutha Venugopal, Sham AanMankunipoyil, C. Manjunatha
Summary: This study investigates the synergistic effect of AgNP and MgO as nanofillers in electrospun Polyurethane. The results show that the addition of MgO improves the physical properties of the nanofibers, while the addition of AgNP enhances the antibacterial activity.
CLOTHING AND TEXTILES RESEARCH JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Sathish Shekhappa Kadapatti, Hosakatte Niranjana Murthy
Summary: A reliable In vitro micropropagation method was developed in Andrographis producta through direct organogenesis, which is helpful for the multiplication, conservation, and utilization of this plant.
JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
(2022)
