Article
Agriculture, Dairy & Animal Science
Dan Mu, Donggen Zhou, Guoyang Xie, Ju Liu, Zhengzheng Wang, Qin Xiong, Hengyi Xu
Summary: In this study, a method for detecting viable E. coli O157:H7 in food products was established using an enhanced nucleic acid binding dye called improved propidium monoazide (PMAxx) coupled with rRAA assay. The PMAxx-rRAA assay showed high sensitivity and good specificity, and could resist interference from dead bacteria and nontarget bacteria.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Food Science & Technology
Alka Rani, Charles Chinyere Dike, Nitin Mantri, Andrew Ball
Summary: This study developed a rapid and reliable method for the detection of viable Escherichia coli O157:H7 using recombinase polymerase amplification (RPA) combined with propidium monoazide (PMAxx). The assay was able to differentiate between viable/dead bacteria and detect VBNC cells. It showed good performance in terms of detection limit, applicability, and pH stability.
Article
Food Science & Technology
Dong Yang, Liang Zhao, Lei Rao, Xiaojun Liao
Summary: This study investigated the effect of various preliminary stresses on high pressure carbon dioxide (HPCD)-induced viable but nonculturable (VBNC) formation in microorganisms. The results showed that heat, acidity, and long-term cultivation preadaptation significantly increased VBNC Escherichia coli production induced by HPCD. Transcriptome analysis revealed a decrease in genes involved in ATP production in stress-treated cells, suggesting that the decrease in ATP caused by these stresses may be the reason for the increased VBNC production. Further experiments confirmed the negative correlation between ATP levels and VBNC ratios. Overall, this study provides guidance for the storage environment of food materials and contributes to the development of HPCD technology in the food industry.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Dong Yang, Yongtao Wang, Liang Zhao, Lei Rao, Xiaojun Liao
Summary: This study explored the roles of extracellular pH and high pressure in inducing viable but non-culturable (VBNC) cells during high pressure carbon dioxide (HPCD) treatment. The results showed that pH had a greater impact on VBNC induction compared to high pressure, and certain genes involved in cellular transport and localization were identified as potential key factors in VBNC induction. Additionally, high pressure was found to accelerate VBNC formation during the HPCD process.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Hanxu Pan, Dong Yang, Yongtao Wang, Lei Rao, Xiaojun Liao
Summary: Under stressful conditions, high pressure carbon dioxide (HPCD) can induce the formation of viable but non-culturable (VBNC) state in E. coli O157:H7. The high expression of acid shock protein Asr coupled with decreased ATP production leads to protein aggregation, ultimately promoting the bacteria to enter VBNC state.
Article
Microbiology
Aifen Ou, Kan Wang, Yanrui Ye, Ling Chen, Xiangjun Gong, Lu Qian, Junyan Liu
Summary: A method using propidium monoazide-crossing priming amplification (PMA-CPA) was developed to detect Salmonella enterica rapidly with high sensitivity and identify VBNC cells in food samples. Specific primers and testing on 20 bacterial strains confirmed the effectiveness and specificity of this method in detecting S. enterica. The successful application of PMA-CPA in detecting S. enterica in VBNC state from food samples was demonstrated with significant color change.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Shuo Zhao, Jingyun Zhang, Zhe Li, Yu Han, Biao Kan
Summary: VBNC cells, a survival strategy adopted by bacterial species like Vibrio cholerae under environmental stress, require accurate methods for identification and enumeration. Quantitative PCR methods, particularly ddPCR, show higher accuracy and sensitivity in counting VBNC cells. PMA-ddPCR method presented in this study offers a new tool for quantifying VBNC cells, which can be extended to other bacterial species.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Elise Richard, Pascale Bourhy, Mathieu Picardeau, Laurent Moulin, Sebastien Wurtzer
Summary: The study introduced a new method, PMAqPCR, which can detect pathogenic and non-pathogenic Leptospira in environmental water while addressing the problem of PCR inhibitors. By testing the killing efficiency of Leptospira to different treatments and comparing PMAqPCR with culture-based enumeration, the research provided insights into the persistence of Leptospira in water treatment processes.
Article
Engineering, Environmental
A. S. Deshpande, N. L. Fahrenfeld
Summary: Shotgun metagenomic studies have examined antibiotic resistance genes (ARGs) in various environments, and combining this method with viability-based techniques can enhance our understanding of ARG hazards. This study evaluated different methods for separating extracellular DNA (eDNA) and putative non-viable cell DNA to investigate their impact on ARG-host assignments. The results showed significant differences in bacterial community structure and ARG profiles between PMA-DNA and total DNA extracts, indicating the importance of sample preparation methods in assessing the potential hazard of riverine ARGs.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Hana Song, Sun-Young Lee
Summary: In this study, Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis entered the viable but nonculturable (VBNC) state under the adverse condition of higher salt concentrations, but regained their culturability when these conditions were reversed.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Article
Food Science & Technology
Junyan Liu, Tengyi Huang, Riyi Xu, Zhufang Xiang, Thanapop Soteyome, Xiangyi Chen, Qianfei Zhang, Qiurong Huang, Zixu Wu, Yunyi Huang, Gongliang Liu, Chunyun Qu, Gamini Seneviratne, Birthe Kjellerup, Zhenbo Xu
Summary: In this study, a PMA-PSR system was designed for accurate determination of viable cells of Escherichia coli O157:H7. The PSR technique was optimized and tested for sensitivity and specificity in pure culture and different food samples. By treating cells with PMA, residual DNA from dead cells was removed, enabling specific detection of viable cells. The system was successfully applied in food samples containing VBNC cells, providing accurate results.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Soil Science
Jing Se, Yulong Fu, Yinan Xie, Fengjun Xu, Chaofeng Shen, Paolo Nannipieri
Summary: The study showed that Escherichia coli O157:H7 can enter the viable but nonculturable state in dry soil, but can revert to culturability and metabolic activity upon soil rewetting. Morphological changes during the VBNC state were associated with differential protein expression.
BIOLOGY AND FERTILITY OF SOILS
(2021)
Article
Food Science & Technology
Jin-Hee Kim, Se-Wook Oh
Summary: By utilizing mPCR-LFA in combination with concentration methods, the sensitivity of detecting Escherichia coli O157:H7 and Salmonella Typhimurium was significantly improved. The study results demonstrate that this approach can rapidly and accurately detect these foodborne pathogens within a short period of time.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Sariqa Wagley, Helen Morcrette, Andrea Kovacs-Simon, Zheng R. Yang, Ann Power, Richard K. Tennant, John Love, Neil Murray, Richard W. Titball, Clive S. Butler
Summary: Members of the Proteobacteria can enter a viable but non culturable (VBNC) state as a survival strategy under stressful conditions. Using Vibrio parahaemolyticus as a model organism, distinct subpopulations in the VBNC state with different resuscitation potentials and proteomic profiles were identified. Deletion of the lactate dehydrogenase gene triggered cells to enter VBNC state, while lactate addition extended resuscitation potential. The ability to survive in VBNC state may be linked to overcoming oxidative stress.
Article
Nutrition & Dietetics
Lisa F. Stinson, Michelle L. Trevenen, Donna T. Geddes
Summary: This study characterized the viable bacterial content and total bacterial DNA content in fresh human milk, finding that the majority of DNA recovered was from non-viable organisms. Samples treated with PMA showed significant differences in bacterial composition compared to untreated samples. These results suggest that the total bacterial DNA content in human milk does not accurately represent the viable human milk microbiome, raising questions about the validity of previous conclusions in this field.
Article
Biotechnology & Applied Microbiology
Junliang Zhong, Xihong Zhao
Review
Food Science & Technology
Junliang Zhong, Xihong Zhao
FOOD ANALYTICAL METHODS
(2018)
Article
Biotechnology & Applied Microbiology
Caijiao Wei, Junliang Zhong, Ting Hu, Xihong Zhao
Article
Biotechnology & Applied Microbiology
Caijiao Wei, Junliang Zhong, Ting Hu, Xihong Zhao
Review
Microbiology
Xihong Zhao, Mei Li, Yao Liu
Article
Microbiology
Junliang Zhong, Xihong Zhao
Review
Microbiology
Xihong Zhao, Zixuan Yu, Tian Ding
Article
Food Science & Technology
Xihong Zhao, Chuncheng Wu
FOOD ANALYTICAL METHODS
(2020)
Review
Food Science & Technology
Rui Gao, Xinyu Liao, Xihong Zhao, Donghong Liu, Tian Ding
Summary: Viable but nonculturable (VBNC) microorganisms are recognized as pathogenic contaminants in foods and environments, with distinct characteristics that have been used as the basis for developing diagnostic tools. Various methods for examining microorganisms in the VBNC state are currently available, each with its own advantages and drawbacks, and there are also emerging techniques with potential for future applications.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2021)
Review
Biotechnology & Applied Microbiology
Zhihui Wang, Xihong Zhao
Summary: The abuse of antibiotics and the emergence of drug-resistant bacteria have worsened food safety. Bacteriophages, a type of virus, have advantages of specificity and non-toxicity. They are increasingly used in the food industry.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Immunology
Shanshan Jin, Yizhe Wang, Xihong Zhao
Summary: This paper reviews the optimal cooling strategies of psychrophilic bacteria and briefly describes the principle of psychrophilic mechanism. It also analyzes the application of thermophilic bacteria and its adaptation to cold environments in the food industry.
MICROBIAL PATHOGENESIS
(2022)
Review
Biochemistry & Molecular Biology
Xiaoli Liu, Huaiying Yao, Xihong Zhao, Chaorong Ge
Summary: Biofilms are aggregation membranes formed by microorganisms on living or nonliving surfaces, providing protection and resistance to antimicrobial agents. If foodborne pathogens form biofilms, the risk of foodborne disease infections can greatly increase, posing significant public health and economic risks. Therefore, research on biofilms and their removal strategies are crucial in the food industry.
Review
Biotechnology & Applied Microbiology
Xueyan Sun, Dongmei Liu, Xihong Zhao
Summary: Macrofungi rely on transcription factors (TFs) to regulate their growth and development processes, such as mycelial growth, fructification, sclerotial formation, sexual reproduction, spore formation, and secondary metabolism. This review comprehensively summarizes the functional TFs in macrofungi and reveals their potential impact mechanisms on growth and development. By providing specific examples, the versatility of TFs in macrofungi is illustrated, and the interaction and possible mechanisms between TFs and macrofungi are discussed for regulating genetic characteristics and gaining new insights into the regulation of growth, development, and metabolism in macrofungi.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yao Liu, Caijiao Wei, Hui Wan, Sarengaowa Sarengaowa, Xiaoping Liang, Tao Jiang, Yuhe Dong, Xihong Zhao, Tian Zhong
Summary: A rapid and practical technique (PMA-mPCR) was developed for the simultaneous detection of viable E. coli O157:H7, S. aureus, and Salmonella in pure culture and in a food matrix. The use of propidium monoazide (PMA) at an optimum concentration of 5.0 μg/mL successfully eliminated false positive results. The PMA-mPCR assay showed a detection limit of 10(4) CFU/mL for both pure culture and food matrix samples.
