Improving the Stability of Cold-Adapted Enzymes by Immobilization

Flexibility and Stability Trade-Off in Active Site of Cold-Adapted Pseudomonas mandelii Esterase EstK

(2016) Ngoc Truongvan et al.   BIOCHEMISTRY

Development of an immobilized biocatalyst based on Bacillus psychrosaccharolyticus NDT for the preparative synthesis of trifluridine and decytabine

(2016) Alba Fresco-Taboada et al.   CATALYSIS TODAY

Defying the activity–stability trade-off in enzymes: taking advantage of entropy to enhance activity and thermostability

(2016) Khawar Sohail Siddiqui   CRITICAL REVIEWS IN BIOTECHNOLOGY

Cloning, expression and characterization of a novel cold-active and organic solvent-tolerant esterase from Monascus ruber M7

(2016) Hailun Guo et al.   EXTREMOPHILES

Immobilization of a novel cold active esterase onto Fe 3 O 4 ∼cellulose nano-composite enhances catalytic properties

(2016) Mohammad Asadur Rahman et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Cold-adapted organic solvent tolerant alkalophilic family I.3 lipase from an Antarctic Pseudomonas

(2016) Menega Ganasen et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Organic solvent-tolerant, cold-adapted lipases PML and LipS exhibit increased conformational flexibility in polar organic solvents

(2016) Vinaykumar Dachuri et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Oligomerization as a strategy for cold adaptation: Structure and dynamics of the GH1 β-glucosidase from Exiguobacterium antarcticum B7

(2016) Leticia Maria Zanphorlin et al.   Scientific Reports

Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes

(2016) Margarita Santiago et al.   Frontiers in Microbiology

Enhanced functionality and stabilization of a cold active laccase using nanotechnology based activation-immobilization

(2015) Arka Mukhopadhyay et al.   BIORESOURCE TECHNOLOGY

Some like it hot, some like it cold: Temperature dependent biotechnological applications and improvements in extremophilic enzymes

(2015) Khawar Sohail Siddiqui   BIOTECHNOLOGY ADVANCES

Application of magnetic nanoparticles in smart enzyme immobilization

(2015) Hamideh Vaghari et al.   BIOTECHNOLOGY LETTERS

Directed Evolution of Enzymes for Industrial Biocatalysis

(2015) Joanne L. Porter et al.   CHEMBIOCHEM

Characterization of a pH and detergent-tolerant, cold-adapted type I pullulanase from Exiguobacterium sp. SH3

(2015) Sarah Rajaei et al.   EXTREMOPHILES

A cold-adapted, solvent and salt tolerant esterase from marine bacterium Psychrobacter pacificensis

(2015) Gaobing Wu et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Nanotechnology based activation-immobilization of psychrophilic pectate lyase: A novel approach towards enzyme stabilization and enhanced activity

(2015) Arka Mukhopadhyay et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

An Organic Solvent-Tolerant Alkaline Lipase from Cold-Adapted Pseudomonas mandelii: Cloning, Expression, and Characterization

(2015) Junsung KIM et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Rational Design, Preparation, and Characterization of a Therapeutic Enzyme Mutant with Improved Stability and Function for Cocaine Detoxification

(2014) Lei Fang et al.   ACS Chemical Biology

Enhanced catalytic site thermal stability of cold-adapted esterase EstK by a W208Y mutation

(2014) Jerusha Boyineni et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Advances in the directed evolution of proteins

(2014) Michael D Lane et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Some like it cold: understanding the survival strategies of psychrophiles

(2014) P. De Maayer et al.   EMBO REPORTS

Nucleoside 2'-Deoxyribosyltransferase from Psychrophilic Bacterium Bacillus psychrosaccharolyticus — Preparation of an Immobilized Biocatalyst for the Enzymatic Synthesis of Therapeutic Nucleosides

(2014) Alba Fresco-Taboada et al.   MOLECULES

Protein Engineering by Random Mutagenesis and Structure-Guided Consensus of Geobacillus stearothermophilus Lipase T6 for Enhanced Stability in Methanol

(2013) Adi Dror et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Screening, purification and characterization of a novel cold-active and organic solvent-tolerant lipase from Stenotrophomonas maltophilia CGMCC 4254

(2013) Mu Li et al.   BIORESOURCE TECHNOLOGY

Increased thermal stability of cold-adapted esterase at ambient temperatures by immobilization on graphene oxide

(2013) Heeyoung Lee et al.   BIORESOURCE TECHNOLOGY

Enzyme immobilisation in biocatalysis: why, what and how

(2013) Roger A. Sheldon et al.   CHEMICAL SOCIETY REVIEWS

Immobilisation and application of lipases in organic media

(2013) Patrick Adlercreutz   CHEMICAL SOCIETY REVIEWS

Magnetic Nanoparticles: Surface Effects and Properties Related to Biomedicine Applications

(2013) Bashar Issa et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Lipase from Yarrowia lipolytica: Production, characterization and application as an industrial biocatalyst

(2013) Ana I.S. Brígida et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Lipase in aqueous-polar organic solvents: Activity, structure, and stability

(2013) Md. Zahid Kamal et al.   PROTEIN SCIENCE

Strategies for Stabilization of Enzymes in Organic Solvents

(2013) Veronika Stepankova et al.   ACS Catalysis

Interactions of graphene and graphene oxide with proteins and peptides

(2013) Yan Zhang et al.   Nanotechnology Reviews

Optimization to Low Temperature Activity in Psychrophilic Enzymes

(2012) Caroline Struvay et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Polar and Alpine Microbial Collection (PAMC): a culture collection dedicated to polar and alpine microorganisms

(2012) Yung Mi Lee et al.   POLAR BIOLOGY

Fluorescence Studies on the Stability, Flexibility and Substrate-Induced Conformational Changes of Acetate Kinases from Psychrophilic and Mesophilic Bacteria

(2012) Md. Abul Kashem Tang et al.   PROTEIN JOURNAL

Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

(2011) Cristina Garcia-Galan et al.   ADVANCED SYNTHESIS & CATALYSIS

Development of effective nanobiocatalytic systems through the immobilization of hydrolases on functionalized carbon-based nanomaterials

(2011) Ioannis V. Pavlidis et al.   BIORESOURCE TECHNOLOGY

Immobilized Candida antarctica lipase B: Hydration, stripping off and application in ring opening polyester synthesis

(2011) Ani Idris et al.   BIOTECHNOLOGY ADVANCES

Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization

(2011) Don A. Cowan et al.   ENZYME AND MICROBIAL TECHNOLOGY

Assembly of Graphene Oxide-Enzyme Conjugates through Hydrophobic Interaction

(2011) Yan Zhang et al.   Small

Biotechnological uses of enzymes from psychrophiles

(2011) R. Cavicchioli et al.   Microbial Biotechnology

The Genome Sequence of Psychrobacter arcticus 273-4, a Psychroactive Siberian Permafrost Bacterium, Reveals Mechanisms for Adaptation to Low-Temperature Growth

(2010) H. L. Ayala-del-Rio et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Biotechnological applications of psychrophiles

(2010) Rosa Margesin et al.   ENVIRONMENTAL TECHNOLOGY

Development of thermostable lipase B from Candida antarctica (CalB) through in silico design employing B-factor and RosettaDesign

(2010) Hyun Sook Kim et al.   ENZYME AND MICROBIAL TECHNOLOGY

Structure and flexibility in cold-adapted iron superoxide dismutases: The case of the enzyme isolated from Pseudoalteromonas haloplanktis

(2010) Antonello Merlino et al.   JOURNAL OF STRUCTURAL BIOLOGY

Graphene Oxide as a Matrix for Enzyme Immobilization

(2010) Jiali Zhang et al.   LANGMUIR

Cross-Linked Enzyme Aggregates as Industrial Biocatalysts

(2010) Roger A. Sheldon   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Structural analysis of thermostabilizing mutations of cocaine esterase

(2010) Diwahar Narasimhan et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Molecular adaptations to psychrophily: the impact of ‘omic’ technologies

(2010) Ana Casanueva et al.   TRENDS IN MICROBIOLOGY

Organic solvent-tolerant enzymes

(2009) Noriyuki Doukyu et al.   BIOCHEMICAL ENGINEERING JOURNAL

Enhancement of the organic solvent-stability of the LST-03 lipase by directed evolution

(2009) Takuya Kawata et al.   BIOTECHNOLOGY PROGRESS

Enzymes from solvent-tolerant microbes: Useful biocatalysts for non-aqueous enzymology

(2009) Anshu Gupta et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

(2009) Roberto Fernandez-Lafuente   ENZYME AND MICROBIAL TECHNOLOGY

Candida antarcticaLipase B Chemically Immobilized on Epoxy-Activated Micro- and Nanobeads: Catalysts for Polyester Synthesis

(2008) Bo Chen et al.   BIOMACROMOLECULES

Cold active microbial lipases: Some hot issues and recent developments

(2008) Babu Joseph et al.   BIOTECHNOLOGY ADVANCES

Protein flexibility in psychrophilic and mesophilic trypsins. Evidence of evolutionary conservation of protein dynamics in trypsin-like serine-proteases

(2008) Elena Papaleo et al.   FEBS LETTERS