A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production

Droplet digital PCR-aided screening and characterization ofPichia pastorismultiple gene copy strains

(2016) Elena Cámara et al.   BIOTECHNOLOGY AND BIOENGINEERING

Comparative genomics and transcriptomics of Pichia pastoris

(2016) Kerry R. Love et al.   BMC GENOMICS

Refined Pichia pastoris reference genome sequence

(2016) Lukas Sturmberger et al.   JOURNAL OF BIOTECHNOLOGY

The PARS sequence increase the efficiency of stable Pichia pastoris transformation

(2016) Claus Krogh Madsen et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris

(2016) Rochelle Aw et al.   Microbial Cell Factories

Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization

(2016) Jan-Philipp Schwarzhans et al.   Microbial Cell Factories

Functional inclusion bodies produced in the yeast Pichia pastoris

(2016) Fabián Rueda et al.   Microbial Cell Factories

Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

(2016) Andrea Camattari et al.   Microbial Cell Factories

Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing

(2016) Jan-Philipp Schwarzhans et al.   Scientific Reports

Cultivation strategies to enhance productivity of Pichia pastoris: A review

(2015) V. Looser et al.   BIOTECHNOLOGY ADVANCES

Genome-scale metabolic model ofPichia pastoriswith native and humanized glycosylation of recombinant proteins

(2015) Zahra Azimzadeh Irani et al.   BIOTECHNOLOGY AND BIOENGINEERING

Systems-level organization of yeast methylotrophic lifestyle

(2015) Hannes Rußmayer et al.   BMC BIOLOGY

Multistep processing of the secretion leader of the extracellular protein Epx1 in Pichia pastoris and implications for protein localization

(2015) Silvia Heiss et al.   MICROBIOLOGY-SGM

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

(2015) Thomas Vogl et al.   ACS Synthetic Biology

Recent advances in the genome-wide study of DNA replication origins in yeast

(2015) Chong Peng et al.   Frontiers in Microbiology

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

GC-Rich DNA Elements Enable Replication Origin Activity in the Methylotrophic Yeast Pichia pastoris

(2014) Ivan Liachko et al.   PLoS Genetics

Playing catch-up with Escherichia coli: using yeast to increase success rates in recombinant protein production experiments

(2014) Roslyn M. Bill   Frontiers in Microbiology

An autonomously replicating sequence for use in a wide range of budding yeasts

(2013) Ivan Liachko et al.   FEMS YEAST RESEARCH

Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

(2013) Roland Prielhofer et al.   Microbial Cell Factories

Can too many copies spoil the broth?

(2013) Rochelle Aw et al.   Microbial Cell Factories

Control of protein synthesis in yeast mitochondria: The concept of translational activators

(2012) Johannes M. Herrmann et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One

GAPPromoter Library for Fine-Tuning of Gene Expression in Pichia pastoris

(2011) Xiulin Qin et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

FIMO: scanning for occurrences of a given motif

(2011) Charles E. Grant et al.   BIOINFORMATICS

Complete genome sequencing of Agrobacterium sp. H13-3, the former Rhizobium lupini H13-3, reveals a tripartite genome consisting of a circular and a linear chromosome and an accessory plasmid but lacking a tumor-inducing Ti-plasmid

(2011) Daniel Wibberg et al.   JOURNAL OF BIOTECHNOLOGY

High-quality genome sequence of Pichia pastoris CBS7435

(2011) Andreas Küberl et al.   JOURNAL OF BIOTECHNOLOGY

Nuclear Mitochondrial DNA Activates Replication in Saccharomyces cerevisiae

(2011) Laurent Chatre et al.   PLoS One

Integrated single-cell analysis showsPichia pastorissecretes protein stochastically

(2010) Kerry Routenberg Love et al.   BIOTECHNOLOGY AND BIOENGINEERING

Real-time PCR-based determination of gene copy numbers inPichia pastoris

(2010) Sandra Abad et al.   Biotechnology Journal

Molecular Poltergeists: Mitochondrial DNA Copies (numts) in Sequenced Nuclear Genomes

(2010) Einat Hazkani-Covo et al.   PLoS Genetics

Super-Resolution Fluorescence Microscopy

(2009) Bo Huang et al.   Annual Review of Biochemistry

A systematical investigation on the genetic stability of multi-copy Pichia pastoris strains

(2009) Taicheng Zhu et al.   BIOTECHNOLOGY LETTERS

Directed gene copy number amplification inPichia pastorisby vector integration into the ribosomal DNA locus

(2009) Hans Marx et al.   FEMS YEAST RESEARCH

Biotechnological strains of Komagataella (Pichia) pastoris are Komagataella phaffii as determined from multigene sequence analysis

(2009) Cletus Paul Kurtzman   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

MEME SUITE: tools for motif discovery and searching

(2009) T. L. Bailey et al.   NUCLEIC ACIDS RESEARCH

Promiscuous DNA in the nuclear genomes of hemiascomycetous yeasts

(2008) Christine Sacerdot et al.   FEMS YEAST RESEARCH

Promoter library designed for fine-tuned gene expression in Pichia pastoris

(2008) Franz S. Hartner et al.   NUCLEIC ACIDS RESEARCH