Promiscuous binding by Hsp70 results in conformational heterogeneity and fuzzy chaperone-substrate ensembles

Alternative modes of client binding enable functional plasticity of Hsp70

(2016) Alireza Mashaghi et al.   NATURE

Structural basis for the antifolding activity of a molecular chaperone

(2016) Chengdong Huang et al.   NATURE

Hsp70 chaperones use ATP to remodel native protein oligomers and stable aggregates by entropic pulling

(2016) Paolo De Los Rios et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation

(2016) Rui Sousa et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Hsp70 biases the folding pathways of client proteins

(2016) Ashok Sekhar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vivo aspects of protein folding and quality control

(2016) D. Balchin et al.   SCIENCE

Hsp70 chaperones use ATP to remodel native protein oligomers and stable aggregates by entropic pulling

(2016) Paolo De Los Rios et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation

(2016) Rui Sousa et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Methyl groups as NMR probes for biomolecular interactions

(2015) Silke Wiesner et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Methyl-specific isotopic labeling: a molecular tool box for solution NMR studies of large proteins

(2015) Rime Kerfah et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

How Hsp70 Molecular Machines Interact with Their Substrates to Mediate Diverse Physiological Functions

(2015) Eugenia M. Clerico et al.   JOURNAL OF MOLECULAR BIOLOGY

Crucial HSP70 co-chaperone complex unlocks metazoan protein disaggregation

(2015) Nadinath B. Nillegoda et al.   NATURE

Heterogeneous binding of the SH3 client protein to the DnaK molecular chaperone

(2015) Jung Ho Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mapping the conformation of a client protein through the Hsp70 functional cycle

(2015) Ashok Sekhar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bringing Dynamic Molecular Machines into Focus by Methyl-TROSY NMR

(2014) Rina Rosenzweig et al.   Annual Review of Biochemistry

Crystal Structure of the Stress-Inducible Human Heat Shock Protein 70 Substrate-Binding Domain in Complex with Peptide Substrate

(2014) Pingfeng Zhang et al.   PLoS One

Single-molecule spectroscopy reveals chaperone-mediated expansion of substrate protein

(2014) Ruth Kellner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Basis for Protein Antiaggregation Activity of the Trigger Factor Chaperone

(2014) T. Saio et al.   SCIENCE

The Cotranslational Function of Ribosome-Associated Hsp70 in Eukaryotic Protein Homeostasis

(2013) Felix Willmund et al.   CELL

Structural Studies on the Forward and Reverse Binding Modes of Peptides to the Chaperone DnaK

(2013) Michael Zahn et al.   JOURNAL OF MOLECULAR BIOLOGY

Allosteric opening of the polypeptide-binding site when an Hsp70 binds ATP

(2013) Ruifeng Qi et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Hsp70 chaperone dynamics and molecular mechanism

(2013) Matthias P. Mayer   TRENDS IN BIOCHEMICAL SCIENCES

An Interdomain Energetic Tug-of-War Creates the Allosterically Active State in Hsp70 Molecular Chaperones

(2012) Anastasia Zhuravleva et al.   CELL

Studying “Invisible” Excited Protein States in Slow Exchange with a Major State Conformation

(2012) Pramodh Vallurupalli et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structure and Dynamics of the ATP-Bound Open Conformation of Hsp70 Chaperones

(2012) Roman Kityk et al.   MOLECULAR CELL

Transient interactions of a slow-folding protein with the Hsp70 chaperone machinery

(2012) Ashok Sekhar et al.   PROTEIN SCIENCE

Protein folding rates and thermodynamic stability are key determinants for interaction with the Hsp70 chaperone system

(2012) Ashok Sekhar et al.   PROTEIN SCIENCE

DnaK Functions as a Central Hub in the E. coli Chaperone Network

(2012) Giulia Calloni et al.   Cell Reports

Visualization and functional analysis of the oligomeric states of Escherichia coli heat shock protein 70 (Hsp70/DnaK)

(2011) Andrea D. Thompson et al.   CELL STRESS & CHAPERONES

Conserved, Disordered C Terminus of DnaK Enhances Cellular Survival upon Stress and DnaKin VitroChaperone Activity

(2011) Robert G. Smock et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Molecular chaperones in protein folding and proteostasis

(2011) F. Ulrich Hartl et al.   NATURE

Single-molecule analysis of a molecular disassemblase reveals the mechanism of Hsc70-driven clathrin uncoating

(2011) Till Böcking et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mechanics of Hsp70 chaperones enables differential interaction with client proteins

(2011) Rainer Schlecht et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins

(2010) Pierre Gans et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A simple strategy for 13C,1H labeling at the Ile-γ2 methyl position in highly deuterated proteins

(2010) Amy M. Ruschak et al.   JOURNAL OF BIOMOLECULAR NMR

Determination of Isoleucine Side-Chain Conformations in Ground and Excited States of Proteins from Chemical Shifts

(2010) D. Flemming Hansen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The kinetic parameters and energy cost of the Hsp70 chaperone as a polypeptide unfoldase

(2010) Sandeep K Sharma et al.   Nature Chemical Biology

Protein dynamics and conformational disorder in molecular recognition

(2009) Tanja Mittag et al.   JOURNAL OF MOLECULAR RECOGNITION

SecB—A chaperone dedicated to protein translocation

(2009) Philipp Bechtluft et al.   Molecular BioSystems

The role of dynamic conformational ensembles in biomolecular recognition

(2009) David D Boehr et al.   Nature Chemical Biology

Solution conformation of wild-type E. coli Hsp70 (DnaK) chaperone complexed with ADP and substrate

(2009) E. B. Bertelsen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Export chaperone SecB uses one surface of interaction for diverse unfolded polypeptide ligands

(2009) Angela A. Lilly et al.   PROTEIN SCIENCE

Molecular Basis for Regulation of the Heat Shock Transcription Factor σ32 by the DnaK and DnaJ Chaperones

(2008) Fernanda Rodriguez et al.   MOLECULAR CELL

The solution structure of the bacterial HSP70 chaperone protein domain DnaK(393-507) in complex with the peptide NRLLLTG

(2003) Shawn Y. Stevens et al.   PROTEIN SCIENCE