CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level

Chromatin accessibility and guide sequence secondary structure affect CRISPR-Cas9 gene editing efficiency

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Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Internal guide RNA interactions interfere with Cas9-mediated cleavage

(2016) Summer B. Thyme et al.   Nature Communications

CRISPR Screens Provide a Comprehensive Assessment of Cancer Vulnerabilities but Generate False-Positive Hits for Highly Amplified Genomic Regions

(2016) D. M. Munoz et al.   Cancer Discovery

Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting

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Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR–Cas9-Mediated Genome Editing in Yarrowia lipolytica

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High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities

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Conformational control of DNA target cleavage by CRISPR–Cas9

(2015) Samuel H. Sternberg et al.   NATURE

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

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High-throughput functional genomics using CRISPR–Cas9

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Short- and long-term effects of chromosome mis-segregation and aneuploidy

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Identification and characterization of essential genes in the human genome

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Enhancers Are Major Targets for Murine Leukemia Virus Vector Integration

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DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

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Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease

(2014) Cem Kuscu et al.   NATURE BIOTECHNOLOGY

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

Improved vectors and genome-wide libraries for CRISPR screening

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Easy quantitative assessment of genome editing by sequence trace decomposition

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The new frontier of genome engineering with CRISPR-Cas9

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DNA targeting specificity of RNA-guided Cas9 nucleases

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RNA-Guided Human Genome Engineering via Cas9

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Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

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A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

The Use of Chromatin Insulators to Improve the Expression and Safety of Integrating Gene Transfer Vectors

(2011) David W. Emery   HUMAN GENE THERAPY

Functional Identification of Optimized RNAi Triggers Using a Massively Parallel Sensor Assay

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The landscape of somatic copy-number alteration across human cancers

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