Engineering peptide ligase specificity by proteomic identification of ligation sites

Targeting the N terminus for site-selective protein modification

(2017) Christian B Rosen et al.   Nature Chemical Biology

Redox-based reagents for chemoselective methionine bioconjugation

(2017) Shixian Lin et al.   SCIENCE

Engineering a Diverse Ligase Toolbox for Peptide Segment Condensation

(2016) Timo Nuijens et al.   ADVANCED SYNTHESIS & CATALYSIS

Enzyme-catalyzed expressed protein ligation

(2016) Samuel H Henager et al.   NATURE METHODS

Six alternative proteases for mass spectrometry–based proteomics beyond trypsin

(2016) Piero Giansanti et al.   Nature Protocols

UniProt: the universal protein knowledgebase

(2016)   NUCLEIC ACIDS RESEARCH

Determination of equilibrium dissociation constants for recombinant antibodies by high-throughput affinity electrophoresis

(2016) Yuchen Pan et al.   Scientific Reports

A High Through-put Platform for Recombinant Antibodies to Folded Proteins

(2015) Michael Hornsby et al.   MOLECULAR & CELLULAR PROTEOMICS

One-step site-specific modification of native proteins with 2-pyridinecarboxyaldehydes

(2015) James I MacDonald et al.   Nature Chemical Biology

Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

(2015) Tino Pleiner et al.   eLife

Self-hydrolyzing maleimides improve the stability and pharmacological properties of antibody-drug conjugates

(2014) Robert P Lyon et al.   NATURE BIOTECHNOLOGY

Butelase 1 is an Asx-specific ligase enabling peptide macrocyclization and synthesis

(2014) Giang K T Nguyen et al.   Nature Chemical Biology

Circulating proteolytic signatures of chemotherapy-induced cell death in humans discovered by N-terminal labeling

(2014) A. P. Wiita et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mechanism, specificity, and physiology of signal peptide peptidase (SPP) and SPP-like proteases

(2013) Matthias Voss et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions

(2013) Jonathan M. Ostrem et al.   NATURE

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

(2013) Carla P Guimaraes et al.   Nature Protocols

A Tobacco Etch Virus Protease with Increased Substrate Tolerance at the P1' position

(2013) Christian Renicke et al.   PLoS One

Reversible targeting of noncatalytic cysteines with chemically tuned electrophiles

(2012) Iana M Serafimova et al.   Nature Chemical Biology

Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction

(2012) Kathrin Lang et al.   Nature Chemistry

Quantitative profiling of caspase-cleaved substrates reveals different drug-induced and cell-type patterns in apoptosis

(2012) K. Shimbo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Caspase Substrates and Cellular Remodeling

(2011) Emily D. Crawford et al.   Annual Review of Biochemistry

Protein N-Terminal Processing: Substrate Specificity ofEscherichia coliand Human Methionine Aminopeptidases

(2010) Qing Xiao et al.   BIOCHEMISTRY

Quantitative reactivity profiling predicts functional cysteines in proteomes

(2010) Eranthie Weerapana et al.   NATURE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Site-specific chemical modification of recombinant proteins produced in mammalian cells by using the genetically encoded aldehyde tag

(2009) P. Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Global Sequencing of Proteolytic Cleavage Sites in Apoptosis by Specific Labeling of Protein N Termini

(2008) Sami Mahrus et al.   CELL

Proteome-derived, database-searchable peptide libraries for identifying protease cleavage sites

(2008) Oliver Schilling et al.   NATURE BIOTECHNOLOGY

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index

(2008) Jagath R Junutula et al.   NATURE BIOTECHNOLOGY