Tying the knot: occurrence and possible significance of gene fusions in plant metabolism and beyond

Essential Role of the Linker Region in the Higher Catalytic Efficiency of a Bifunctional MsrA–MsrB Fusion Protein

(2016) Ah-reum Han et al.   BIOCHEMISTRY

Interdomain Conformational Changes Provide Allosteric Regulation en Route to Chorismate

(2016) Ali Reza Nazmi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Heterologous production of raspberry ketone in the wine yeast Saccharomyces cerevisiae via pathway engineering and synthetic enzyme fusion

(2016) Danna Lee et al.   Microbial Cell Factories

Plant metabolism, the diverse chemistry set of the future

(2016) E. T. Wurtzel et al.   SCIENCE

Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum

(2016) Tomas Laursen et al.   SCIENCE

Natural Products and the Gene Cluster Revolution

(2016) Paul R. Jensen   TRENDS IN MICROBIOLOGY

Genes encoding norcoclaurine synthase occur as tandem fusions in the Papaveraceae

(2016) Jing Li et al.   Scientific Reports

Overexpression of a Chimeric Gene, OsDST-SRDX, Improved Salt Tolerance of Perennial Ryegrass

(2016) Huifang Cen et al.   Scientific Reports

HpDTC1, a Stress-Inducible Bifunctional Diterpene Cyclase Involved in Momilactone Biosynthesis, Functions in Chemical Defence in the Moss Hypnum plumaeforme

(2016) Kazunori Okada et al.   Scientific Reports

Evolutionary origin of the NCSI gene subfamily encoding norcoclaurine synthase is associated with the biosynthesis of benzylisoquinoline alkaloids in plants

(2016) Sornkanok Vimolmangkang et al.   Scientific Reports

Synthetic fusion protein design and applications

(2015) Kai Yu et al.   BIOTECHNOLOGY ADVANCES

Evolution and tinkering: what do a protein kinase, a transcriptional regulator and chromosome segregation/cell division proteins have in common?

(2015) Abderahmane Derouiche et al.   CURRENT GENETICS

‘Dopamine-first’ mechanism enables the rational engineering of the norcoclaurine synthase aldehyde activity profile

(2015) Benjamin R. Lichman et al.   FEBS Journal

Stereochemical inversion of (S)-reticuline by a cytochrome P450 fusion in opium poppy

(2015) Scott C Farrow et al.   Nature Chemical Biology

New genes from non-coding sequence: the role of de novo protein-coding genes in eukaryotic evolutionary innovation

(2015) Aoife McLysaght et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

A lycopene β-cyclase/lycopene ε-cyclase/light-harvesting complex-fusion protein from the green algaOstreococcus lucimarinuscan be modified to produce α-carotene and β-carotene at different ratios

(2015) Andreas Blatt et al.   PLANT JOURNAL

Analysis of Loss-of-Function Mutants inAspartate KinaseandHomoserine DehydrogenaseGenes Points to Complexity in the Regulation of Aspartate-Derived Amino Acid Contents

(2015) Teresa J. Clark et al.   PLANT PHYSIOLOGY

Morphinan biosynthesis in opium poppy requires a P450-oxidoreductase fusion protein

(2015) T. Winzer et al.   SCIENCE

Increased production of wax esters in transgenic tobacco plants by expression of a fatty acid reductase:wax synthase gene fusion

(2015) Selcuk Aslan et al.   TRANSGENIC RESEARCH

Construction, expression, and characterization of Arabidopsis thaliana 4CL and Arachis hypogaea RS fusion gene 4CL::RS in Escherichia coli

(2015) Erhao Zhang et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

To Gibberellins and Beyond! Surveying the Evolution of (Di)Terpenoid Metabolism

(2014) Jiachen Zi et al.   Annual Review of Plant Biology

Bringing functions together with fusion enzymes—from nature’s inventions to biotechnological applications

(2014) Skander Elleuche   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Long Noncoding RNA Modulates Alternative Splicing Regulators in Arabidopsis

(2014) Florian Bardou et al.   DEVELOPMENTAL CELL

The Bifunctional Protein TtFARAT fromTetrahymena thermophilaCatalyzes the Formation of both Precursors Required to Initiate Ether Lipid Biosynthesis

(2014) Franziska Dittrich-Domergue et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

First Evidence for Substrate Channeling between Proline Catabolic Enzymes

(2014) Nikhilesh Sanyal et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Wax esters of different compositions produced via engineering of leaf chloroplast metabolism in Nicotiana benthamiana

(2014) Selcuk Aslan et al.   METABOLIC ENGINEERING

The fused anthranilate synthase from Streptomyces venezuelae functions as a monomer

(2014) Meseret Ashenafi et al.   MOLECULAR AND CELLULAR BIOCHEMISTRY

Structures of the PutA peripheral membrane flavoenzyme reveal a dynamic substrate-channeling tunnel and the quinone-binding site

(2014) Harkewal Singh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The rise of operon-like gene clusters in plants

(2014) Svetlana Boycheva et al.   TRENDS IN PLANT SCIENCE

New Gene Evolution: Little Did We Know

(2013) Manyuan Long et al.   Annual Review of Genetics

First Three-Dimensional Structure of Toxoplasma gondii Thymidylate Synthase–Dihydrofolate Reductase: Insights for Catalysis, Interdomain Interactions, and Substrate Channeling

(2013) Hitesh Sharma et al.   BIOCHEMISTRY

The shikimate pathway: Review of amino acid sequence, function and three-dimensional structures of the enzymes

(2013) Rafia Mir et al.   CRITICAL REVIEWS IN MICROBIOLOGY

Gene clustering in plant specialized metabolism

(2013) Hans-Wilhelm Nützmann et al.   CURRENT OPINION IN BIOTECHNOLOGY

Evidence for Hysteretic Substrate Channeling in the Proline Dehydrogenase and Δ1-Pyrroline-5-carboxylate Dehydrogenase Coupled Reaction of Proline Utilization A (PutA)

(2013) Michael A. Moxley et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Fusion of Dioxygenase and Lignin-binding Domains in a Novel Secreted Enzyme from CellulolyticStreptomycessp. SirexAA-E

(2013) Christopher M. Bianchetti et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A metabolic gene cluster inLotus japonicusdiscloses novel enzyme functions and products in triterpene biosynthesis

(2013) Afrodite Krokida et al.   NEW PHYTOLOGIST

Benzylisoquinoline Alkaloid Metabolism: A Century of Discovery and a Brave New World

(2013) Jillian M. Hagel et al.   PLANT AND CELL PHYSIOLOGY

The Shikimate Pathway and Aromatic Amino Acid Biosynthesis in Plants

(2012) Hiroshi Maeda et al.   Annual Review of Plant Biology

Biological Channeling of a Reactive Intermediate in the Bifunctional Enzyme DmpFG

(2012) Natalie E. Smith et al.   BIOPHYSICAL JOURNAL

A Gene-Fusion Approach to Enabling Plant Cytochromes P450 for Biocatalysis

(2012) Julia Schückel et al.   CHEMBIOCHEM

Comparative genomics approaches to understanding and manipulating plant metabolism

(2012) Louis MT Bradbury et al.   CURRENT OPINION IN BIOTECHNOLOGY

Subcellular localization of sanguinarine biosynthetic enzymes in cultured opium poppy cells

(2012) Jillian M. Hagel et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Arabidopsis RIBA Proteins: Two out of Three Isoforms Have Lost Their Bifunctional Activity in Riboflavin Biosynthesis

(2012) Hanna-Maija Hiltunen et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Insights into Diterpene Cyclization from Structure of Bifunctional Abietadiene Synthase fromAbies grandis

(2012) Ke Zhou et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The tomato genome sequence provides insights into fleshy fruit evolution

(2012)   NATURE

Biochemical and Structural Characterization of theArabidopsisBifunctional Enzyme Dethiobiotin Synthetase–Diaminopelargonic Acid Aminotransferase: Evidence for Substrate Channeling in Biotin Synthesis

(2012) David Cobessi et al.   PLANT CELL

A Papaver somniferum 10-Gene Cluster for Synthesis of the Anticancer Alkaloid Noscapine

(2012) T. Winzer et al.   SCIENCE

Birth, death and subfunctionalization in the Arabidopsis genome

(2012) Matthew T. Rutter et al.   TRENDS IN PLANT SCIENCE

Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779

(2012) Astrid Vieler et al.   PLoS Genetics

A nucleotide insertion between two adjacent methyltransferases inHelicobacter pyloriresults in a bifunctional DNA methyltransferase

(2011) Ritesh Kumar et al.   BIOCHEMICAL JOURNAL

Investigating the Molecular Determinants for Substrate Channeling in BphI–BphJ, an Aldolase–Dehydrogenase Complex from the Polychlorinated Biphenyls Degradation Pathway

(2011) Jason Carere et al.   BIOCHEMISTRY

Evolutionary origins of Brassicaceae specific genes in Arabidopsis thaliana

(2011) Mark TA Donoghue et al.   BMC EVOLUTIONARY BIOLOGY

Investigating conservation of the albaflavenone biosynthetic pathway and CYP170 bifunctionality in streptomycetes

(2011) Suzy C. Moody et al.   FEBS Journal

Glutathione Biosynthesis in Bacteria by Bifunctional GshF Is Driven by a Modular Structure Featuring a Novel Hybrid ATP-Grasp Fold

(2011) Jan Stout et al.   JOURNAL OF MOLECULAR BIOLOGY

The family of terpene synthases in plants: a mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom

(2011) Feng Chen et al.   PLANT JOURNAL

Formation of plant metabolic gene clusters within dynamic chromosomal regions

(2011) B. Field et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Overexpression of an HPS/PHI fusion enzyme from Mycobacterium gastri in chloroplasts of geranium enhances its ability to assimilate and phytoremediate formaldehyde

(2010) Zhongbang Song et al.   BIOTECHNOLOGY LETTERS

Origins, evolution, and phenotypic impact of new genes

(2010) H. Kaessmann   GENOME RESEARCH

Overproduction of Alkaloid Phytoalexins in California Poppy Cells Is Associated with the Co-Expression of Biosynthetic and Stress-Protective Enzymes

(2010) Sornitza Angelova et al.   Molecular Plant

Norcoclaurine Synthase Is a Member of the Pathogenesis-Related 10/Bet v1 Protein Family

(2010) Eun-Jeong Lee et al.   PLANT CELL

Crystal structure of the bifunctional proline utilization A flavoenzyme from Bradyrhizobium japonicum

(2010) D. Srivastava et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Gibberellin biosynthesis in bacteria: Separateent-copalyl diphosphate andent-kaurene synthases inBradyrhizobium japonicum

(2009) Dana Morrone et al.   FEBS LETTERS

Threonine-insensitive Homoserine Dehydrogenase from Soybean

(2009) Amy C. Schroeder et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

CYP719B1 Is Salutaridine Synthase, the C-C Phenol-coupling Enzyme of Morphine Biosynthesis in Opium Poppy

(2009) Andreas Gesell et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Molecular Phylogeny and Classification of the Mamiellophyceae class. nov. (Chlorophyta) based on Sequence Comparisons of the Nuclear- and Plastid-encoded rRNA Operons

(2009) Birger Marin et al.   PROTIST

Structural Basis of Enzymatic (S)-Norcoclaurine Biosynthesis

(2008) Andrea Ilari et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Chloroplast Genomes of the Green Algae Pyramimonas, Monomastix, and Pycnococcus Shed New light on the Evolutionary History of Prasinophytes and the Origin of the Secondary Chloroplasts of Euglenids

(2008) Monique Turmel et al.   MOLECULAR BIOLOGY AND EVOLUTION

Amino acid biosynthesis: New architectures in allosteric enzymes

(2008) Gilles Curien et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY