Review
Biochemistry & Molecular Biology
Thomas A. DeFalco, Cyril Zipfel
Summary: All eukaryotic organisms have developed sophisticated immune systems to combat biotic stresses, with PRRs being a key part in this system. PRRs sense molecular patterns to regulate PTI, with notable progress in understanding how they activate signaling. PRR complex formation and phosphorylation play critical roles in activating PTI signaling, with RLCKs acting as executors downstream of PRR activation.
Article
Cell Biology
Ling-Chang Tong, Zhi-Bin Wang, Jia-Qi Zhang, Yue Wang, Wei-Ye Liu, Hao Yin, Jia-Cheng Li, Ding-Feng Su, Yong-Bing Cao, Li-Chao Zhang, Ling Li
Summary: The study revealed that swiprosin-1 expressed in macrophages plays a key role in atherosclerosis development. Lack of swiprosin-1 can alleviate the progression of AS, making it a promising new therapeutic target.
CELL DEATH DISCOVERY
(2021)
Article
Immunology
Waipan Chan, Yuqi M. Cao, Xiang Zhao, Edward C. Schrom, Dongya Jia, Jian Song, Leah V. Sibener, Shen Dong, Ricardo A. Fernandes, Clinton J. Bradfield, Margery Smelkinson, Juraj Kabat, Jyh Liang Hor, Gregoire Altan-Bonnet, K. Christopher Garcia, Ronald N. Germain
Summary: This study developed a fluorescent intracellular live multiplex signal transduction activity reporter system to analyze the suppressive effects of PD-1. The findings suggest that PD-1 primarily targets TCR-linked signals in a manner highly sensitive to peptide ligand quality. These findings help resolve existing controversies about PD-1 inhibition in T cells and emphasize the importance of neoantigen potency.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Dongqin Chen, Fengsheng Hao, Huiqi Mu, Nagib Ahsan, Jay J. Thelen, Gary Stacey
Summary: The study investigates how S-acylation regulates plant innate immunity by modulating the activity of immune receptors. It was found that S-acylation can influence the phosphorylation and degradation processes of the P2K1 receptor, affecting the intensity of immune responses.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Shaoyan Gao, Xiaohe Li, Qiuyan Jiang, Qing Liang, Fangxia Zhang, Shuangling Li, Ruiqin Zhang, Jiaoyan Luan, Jingyan Zhu, Xiaoting Gu, Ting Xiao, Hui Huang, Shanshan Chen, Wen Ning, Guang Yang, Cheng Yang, Honggang Zhou
Summary: Our study demonstrates that PKM2 promotes fibrosis progression by interacting with Smad7 and enhancing TGF-beta 1 signaling. Increased expression of PKM2 in the lungs of mice with pulmonary fibrosis contributes to fibrosis development. The PKM2 tetramer enhances TGF-beta 1 signaling by directly binding with Smad7, making it a key factor in fibrosis progression.
Article
Cell Biology
Chunye Liu, Changdong Lin, Daisong Wang, Jingqiang Wang, Yu Tao, Yue Li, Xinyi Chen, Lanyue Bai, Yingying Jia, Jianfeng Chen, Yi Arial Zeng
Summary: This study demonstrates the essential role of Procr in mammary gland development and homeostasis. It acts as a signaling receptor of protein C and interacts with HSP90, Src, and IGF1R to regulate MaSCs.
Article
Cell Biology
Daniel B. Goodman, Camillia S. Azimi, Kendall Kearns, Alexis Talbot, Kiavash Garakani, Julie Garcia, Nisarg Patel, Byungjin Hwang, David Lee, Emily Park, Vivasvan S. Vykunta, Brian R. Shy, Chun Jimmie Ye, Justin Eyquem, Alexander Marson, Jeffrey A. Bluestone, Kole T. Roybal
Summary: CAR Pooling is a multiplexed approach to rapidly identify CAR designs with clinical potential by evaluating the functionality of CARs with different signaling domains under prolonged antigen stimulation, aiming to improve the efficacy of CAR T cell therapies.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Rafael Deliz-Aguirre, Fakun Cao, Fenja H. U. Gerpott, Nichanok Auevechanichkul, Mariam Chupanova, YeVin Mun, Elke Ziska, Marcus J. Taylor
Summary: Oligomerization of MyD88 is inducible and initially reversible. Formation of larger, stable oligomers triggers sequential recruitment of IRAK4 and IRAK1, with IRAK4 controlling MyD88 oligomer size and growth. MyD88 oligomer size functions as a physical threshold to trigger downstream signaling pathways.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rupashree Salvi, Chandan Kumar, Krupanshi Brahmbhatt, Rambhadur Subedi, Susan Idicula-Thomas, Taruna Madan, Barnali Biswas
Summary: The study demonstrates differential cell surface proteome effects and phosphorylation levels in CHO mutant cells with loss of N-glycosylation function. Specifically, the abundance of integrin subunits is increased while IGF-1R and IQGAP1 levels are reduced on the cell surface of Lec1 and Lec4 cells. The study also reveals a novel mechanism of IQGAP1 as an IGF-1R-associated scaffold protein in activating IGF-1 dependent ERK signaling in CHO cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Kuan-Yin Tseng, Vassilis Stratoulias, Wei-Fen Hu, Jui-Sheng Wu, Vicki Wang, Yuan-Hao Chen, Anna Seelbach, Henri J. Huttunen, Natalia Kulesskaya, Cheng-Yoong Pang, Jian-Liang Chou, Maria Lindahl, Mart Saarma, Li-Chuan Huang, Mikko Airavaara, Hock-Kean Liew
Summary: During intracerebral hemorrhage (ICH), hematoma formation leads to local mechanical injury, and the release of hemoglobin and heme induces inflammation and secondary brain injury. Modulating immune cells to promote hematoma resorption and reduce brain edema has potential as a therapeutic strategy for functional recovery after ICH.
CELL DEATH & DISEASE
(2023)
Review
Immunology
Charitha Madiraju, Jeffrey P. Novack, John C. Reed, Shu-ichi Matsuzawa
Summary: This review highlights the significance of K63 ubiquitination in immune signaling and its role in regulating various immune functions. It provides insights into the mechanisms by which pathogens, including SARS-CoV-2, suppress immune responses through targeting K63 ubiquitination. The review also discusses recent findings suggesting the involvement of K63 ubiquitination in the regulation of regulatory T cells and T helper cell subsets, and identifies knowledge gaps for future investigation and clinically relevant pathways.
TRENDS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Citlaly Gutierrez-Rodelo, Araceli Arellano-Plancarte, Judith Hernandez-Aranda, Huguet Landa-Galvan, G. Karina Parra-Mercado, Nicole J. Moreno-Licona, Karla D. Hernandez-Gonzalez, Kevin J. Catt, Rafael Villalobos-Molina, J. Alberto Olivares-Reyes
Summary: Angiotensin II plays a critical role in regulating insulin signaling in the cardiovascular system and metabolic tissues. However, in adipose cells, it inhibits insulin actions through activation of PKC, leading to insulin resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Tina Arkee, Bruce S. Hostager, Jon C. D. Houtman, Gail A. Bishop
Summary: TRAF3 plays a crucial role in enhancing T cell effector functions and TCR/CD28 signaling by restraining the negative regulation of LAT through inhibition of Dok1 and regulation of Brk activation, ultimately promoting Src family kinase activation. This interaction between TRAF3 and LAT sheds light on a novel mechanism for promoting TCR/CD28-mediated signaling.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Cell Biology
Effie E. Bastounis, Francisco Serrano-Alcalde, Prathima Radhakrishnan, Patrik Engstrom, Maria J. Gomez-Benito, Mackenzi S. Oswald, Yi-Ting Yeh, Jason G. Smith, Matthew D. Welch, Jose M. Garcia-Aznar, Julie A. Theriot
Summary: Intracellular pathogens alter the mechanics of host cells to promote dissemination, while host cells respond by collectively eliminating infected cells to limit infection, indicating an innate immunity-driven process.
DEVELOPMENTAL CELL
(2021)
Article
Biochemistry & Molecular Biology
Iram Fatima, Susmita Barman, JayaPrakash Uppada, Shailender Chauhan, Sanchita Rauth, Satyanarayana Rachagani, Moorthy Palanimuthu Ponnusamy, Lynette Smith, Geoffrey Talmon, Amar B. Singh, Surinder K. Batra, Punita Dhawan
Summary: This study reveals a critical role of MASTL in promoting pancreatic cancer malignancy and therapy resistance by modulating protein stability and kinase signaling. Combinatorial therapy targeting MASTL enhances the cell-killing effects of Gemcitabine, demonstrating its potential as a novel target to improve sensitivity to anti-pancreatic cancer therapies.
Article
Cardiac & Cardiovascular Systems
Christian U. Oeing, Taishi Nakamura, Shi Pan, Sumita Mishra, Brittany L. Dunkerly-Eyring, Kristen M. Kokkonen-Simon, Brian L. Lin, Anna Chen, Guangshuo Zhu, Djahida Bedja, Dong Ik Lee, David A. Kass, Mark J. Ranek
CIRCULATION RESEARCH
(2020)
Review
Physiology
Christian U. Oeing, Sumita Mishra, Brittany L. Dunkerly-Eyring, Mark J. Ranek
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Allergy
Yan Zhang, Danh C. Do, Xinyue Hu, Ji Wang, Yilin Zhao, Sumita Mishra, Xin Zhang, Mei Wan, Peisong Gao
Summary: The study identified that cockroach allergen activates autophagy, while inhibition of autophagy can mitigate airway hyperresponsiveness and oxidative stress induced by allergic airway inflammation. Furthermore, the research revealed a pathological feedforward circuit between cockroach allergen-induced ROS and autophagy.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Mark J. Ranek, Christian Oeing, Rebekah Sanchez-Hodge, Kristen M. Kokkonen-Simon, Danielle Dillard, M. Imran Aslam, Peter P. Rainer, Sumita Mishra, Brittany Dunkerly-Eyring, Ronald J. Holewinski, Cornelia Virus, Huaqun Zhang, Matthew M. Mannion, Vineet Agrawal, Virginia Hahn, Dong I. Lee, Masayuki Sasaki, Jennifer E. Van Eyk, Monte S. Willis, Richard C. Page, Jonathan C. Schisler, David A. Kass
NATURE COMMUNICATIONS
(2020)
Article
Cardiac & Cardiovascular Systems
Raihan Kabir, Prithvi Sinha, Sumita Mishra, Obialunanma Ebenebe, Nicole Taube, Chistian U. Oeing, Gizem Keceli, Rui Chen, Nazareno Paolocci, Ana Rule, Mark J. Kohr
Summary: This study establishes a mechanistic link between environmentally relevant doses of inorganic arsenic (iAS) and pathological hypertrophy in the heart. Results show that iAS exposure may induce pathological cardiac hypertrophy not only by increasing systolic blood pressure, but also by potentially activating calcineurin-nuclear factor of activated T cells and inducing fetal gene expression. These findings provide new mechanistic insights into the threat of iAS exposure to the heart, necessary for identifying targets for medical and public health interventions.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Christian U. Oeing, Seungho Jun, Sumita Mishra, Brittany L. Dunkerly-Eyring, Anna Chen, Maria Grajeda, Usman A. Tahir, Robert E. Gerszten, Nazareno Paolocci, Mark J. Ranek, David A. Kass
Summary: The phosphorylation status of TSC2-S1365 plays a crucial role in regulating myocardial substrate utilization, activating mTORC1 to shift metabolism away from fatty acid oxidation towards glycolysis for protection against ischemia-reperfusion injury. Reduced phosphorylation of TSC2-S1365 is also required for effective ischemic preconditioning (IPC) process.
CIRCULATION RESEARCH
(2021)
Correction
Cardiac & Cardiovascular Systems
Sumita Mishra, David A. Kass
Summary: The correction to this paper has been published.
NATURE REVIEWS CARDIOLOGY
(2021)
Article
Endocrinology & Metabolism
Ryan P. Ceddia, Dianxin Liu, Fubiao Shi, Mark K. Crowder, Sumita Mishra, David A. Kass, Sheila Collins
Summary: The inhibition of PDE9 was found to increase energy expenditure and improve glucose handling and hepatic steatosis in mice fed with high-fat diets. This suggests that PDE9 plays a crucial role in energy metabolism and its inhibition may be a potential avenue for combating metabolic diseases.
Article
Biology
Brittany L. Dunkerly-Eyring, Shi Pan, Miguel Pinilla-Vera, Desirae McKoy, Sumita Mishra, Maria I. Grajeda Martinez, Christian U. Oeing, Mark J. Ranek, David A. Kass
Summary: Serine 1364 on TSC2 plays a role in regulating mTORC1 activation through ERK1/2 phosphorylation but not Akt activation. Phospho-mimetic and phospho-silenced mutations at S1364 both affect mTORC1 activation. S1364 has negligible impact on mTORC1 regulation by energy and nutrient status.
LIFE SCIENCE ALLIANCE
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Sumita Mishra, Junfeng Ma, Kei Akiyoshi, Federica Farinelli, Rick Page, Christian U. Oeing, Natasha Zachara, Samarjit Das, Mark J. Ranek, David A. Kass
CIRCULATION RESEARCH
(2021)
Article
Medicine, Research & Experimental
Sumita Mishra, Nandhini Sadagopan, Brittany Dunkerly-Eyring, Susana Rodriguez, Dylan C. Sarver, Ryan P. Ceddia, Sean A. Murphy, Hildur Knutsdottir, Vivek P. Jani, Deepthi Ashok, Christian U. Oeing, Brian O'Rourke, Jon A. Gangoiti, Dorothy D. Sears, G. William Wong, Sheila Collins, David A. Kass
Summary: Inhibition of PDE9 shows promising effects in improving obesity and cardiometabolic syndrome symptoms, especially in male and ovariectomized female mice. PDE9-I stimulates mitochondrial activity in brown and white fat, reduces various fat deposits, and improves CMS without significantly affecting activity or food intake.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Meeting Abstract
Cardiac & Cardiovascular Systems
Raihan Kabir, Prithvi Sinha, Sumita Mishra, Obialunanma V. Ebenebe, Nicole Taube, Christian Oening, Gizem Keceli, Rui Chen, Nazareno Paolocci, Ana M. Rule, Mark J. Kohr
Meeting Abstract
Cardiac & Cardiovascular Systems
Christian U. Oeing, Seungho Jun, Sumita Mishra, Brittany L. Dunkerly-eyring, Anna Chen, Maria I. Grajeda, Usman A. Tahir, Robert E. Gerszten, Nazareno Paolocci, Mark J. Ranek, David A. Kass
CIRCULATION RESEARCH
(2020)
Review
Physiology
Sumita Mishra, Brittany L. Dunkerly-Eyring, Gizem Keceli, Mark J. Ranek
FRONTIERS IN PHYSIOLOGY
(2020)
Meeting Abstract
Cardiac & Cardiovascular Systems
Christian U. Oeing, Seungho Jun, Brittany Dunkerly-Eyring, Sumita Mishra, Usman Tahir, Guangshuo Zhu, Gizem Keceli, Robert Gerszten, Mark Ranek, Nazareno Paolocci, David Kass
