Article
Multidisciplinary Sciences
Ryuhei Hayashi, Toru Okubo, Yuji Kudo, Yuki Ishikawa, Tsutomu Imaizumi, Kenji Suzuki, Shun Shibata, Tomohiko Katayama, Sung-Joon Park, Robert D. Young, Andrew J. Quantock, Kohji Nishida
Summary: This study identifies cells with characteristics of lacrimal gland primordia in three-dimensional eye-like organoids cultured from human pluripotent stem cells. These cells form a lacrimal-gland-like tissue organoid with ducts and acini through budding and branching when isolated by cell sorting and grown under defined conditions. The organoids originate from multipotent ocular surface epithelial stem cells and exhibit similarities to native lacrimal glands in morphology, immunolabeling characteristics, and gene expression patterns. When transplanted adjacent to the eyes of recipient rats, the organoids undergo functional maturation, developing lumina and producing tear-film proteins.
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cell Biology
Shin-Jeong Lee, Cholomi Jung, Jee Eun Oh, Sangsung Kim, Sangho Lee, Ji Yoon Lee, Young-sup Yoon
Summary: Red blood cell (RBC) transfusion is a lifesaving procedure for anemia and hemoglobin disorders, but faces challenges due to the shortage of blood supply and risks of infection and incompatibility. The use of human pluripotent stem cells (hPSCs), including human induced pluripotent stem cells (hiPSCs), holds promise for generating RBCs. This review discusses the concepts and mechanisms of erythropoiesis, methodologies for differentiating hPSCs into erythrocytes, and the limitations and future directions of using hiPSC-derived erythrocytes in clinical applications.
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Article
Biochemistry & Molecular Biology
Pablo Hofbauer, Stefan M. Jahnel, Nora Papai, Magdalena Giesshammer, Alison Deyett, Clara Schmidt, Mirjam Penc, Katherina Tavernini, Nastasja Grdseloff, Christy Meledeth, Lavinia Ceci Ginistrelli, Claudia Ctortecka, Sejla Salic, Maria Novatchkova, Sasha Mendjan
Summary: Organoids derived from human pluripotent stem cells can spontaneously form chamber-like structures resembling the human heart, which can be controlled and manipulated for studying heart diseases and regeneration. The establishment of self-organizing cardioids provides a powerful platform for mechanistic studies on developmental defects and potential translational research in the future.
Article
Biochemical Research Methods
Min Shi, Ping Fu, Joseph V. Bonventre, Kyle W. McCracken
Summary: Developing in vitro models of human kidney tissue using human pluripotent stem cells (hPSCs) is challenging but essential for regenerative nephrology research. This article describes a step-by-step protocol for differentiating hPSCs into three-dimensional ureteric bud (UB) organoids, which exhibit complex morphological development and can differentiate into functional collecting duct tissues. This approach provides a robust and reproducible method for generating UB tissues that can be used to study kidney development, model disease processes, and aid in the engineering of functional kidney tissue.
Review
Cell & Tissue Engineering
Kevin Hayes, Yun-Kyo Kim, Martin F. Pera
Summary: Nodal is a crucial factor in mammalian embryonic development and the maintenance of pluripotent stem cells. Its signaling pathway may play an important role in regulating self-renewal in hPSC.
Article
Multidisciplinary Sciences
Leqian Yu, Yulei Wei, Jialei Duan, Daniel A. Schmitz, Masahiro Sakurai, Lei Wang, Kunhua Wang, Shuhua Zhao, Gary C. Hon, Jun Wu
Summary: The study developed an effective three-dimensional culture strategy to generate blastocyst-like structures in vitro which resemble human blastocysts. These structures, termed 'human blastoids', are readily accessible, scalable, versatile, and perturbable alternative to blastocysts for studying early human development, understanding early pregnancy loss, and gaining insights into early developmental defects.
Article
Chemistry, Multidisciplinary
Dhiraj Indana, Pranay Agarwal, Nidhi Bhutani, Ovijit Chaudhuri
Summary: This research utilized viscoelastic alginate hydrogels to study hiPSC morphogenesis in 3D culture. Higher RGD density and fast stress relaxation were found to promote hiPSC viability, proliferation, and lumen formation, while slow stress relaxation at low RGD densities triggered hiPSC apoptosis.
ADVANCED MATERIALS
(2021)
Article
Cell & Tissue Engineering
Chuti Laowtammathron, Chanchao Lorthongpanich, Nittaya Jiamvoraphong, Pimonwan Srisook, Phatchanat Klaihmon, Pakpoom Kheolamai, Sudjit Luanpitpong, Surapol Issaragrisil
Summary: The study explores the role of YAP in hematopoietic differentiation of hiPSCs. It is found that suppressing YAP activity enhances the efficiency of hematopoietic differentiation and increases the yield of HSPCs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Lauren Michelle Walker, Nicole R. L. Sparks, Veronica Puig-Sanvicens, Beatriz Rodrigues, Nicole zur Nieden
Summary: This study updated the classic mouse EST with endpoints derived from human induced pluripotent stem cells (hiPSCs) and human fibroblasts, showing improved prediction of human risks; the hiPSC-EST was able to detect inhibition of differentiation at lower concentrations and adverse developmental outcomes caused by novel environmental toxicants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Magdalena Schindler, Dylan Siriwardena, Timo N. Kohler, Anna L. Ellermann, Erin Slatery, Clara Munger, Florian Hollfelder, Thorsten E. Boroviak
Summary: The research established a high-throughput in vitro model by encapsulating human pluripotent stem cells into monodisperse agarose microgels. Encapsulated primed hPSCs required Rho-associated kinase inhibition, in contrast to naive hPSCs. The study revealed an increase in lumenogenesis during the naive-to-primed transition.
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Article
Biochemical Research Methods
Jiyoon Lee, Wouter H. van der Valk, Sara A. Serdy, CiCi Deakin, Jin Kim, Anh Phuong Le, Karl R. Koehler
Summary: This article describes a detailed protocol for generating hair-bearing skin tissue from human pluripotent stem cells. By using defined culture conditions in a three-dimensional in vitro culture system, skin organoids with hair follicles can be obtained after 60 days and reach full complexity after approximately 130 days.
Review
Biochemistry & Molecular Biology
Ines Milagre, Carolina Pereira, Raquel A. Oliveira
Summary: Human pluripotent stem cells (PSCs), including embryonic and induced pluripotent stem cells, are widely used in biomedical research for understanding development, disease progression, and drug discovery. However, concerns have been raised regarding their safety in regenerative medicine, particularly due to the occurrence of aneuploid cells resulting from errors in chromosome segregation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Feng Lin, Yue Shao, Xufeng Xue, Yi Zheng, Zida Li, Chunyang Xiong, Jianping Fu
ACTA BIOMATERIALIA
(2019)
Article
Chemistry, Multidisciplinary
Weiqiang Chen, Steven G. Allen, Weiyi Qian, Zifeng Peng, Shuo Han, Xiang Li, Yubing Sun, Chelsea Fournier, Liwei Bao, Raymond H. W. Lam, Sofia D. Merajver, Jianping Fu
Article
Instruments & Instrumentation
Abhay Kotnala, Yi Zheng, Jianping Fu, Wei Cheng
REVIEW OF SCIENTIFIC INSTRUMENTS
(2019)
Article
Multidisciplinary Sciences
Yi Zheng, Xufeng Xue, Yue Shao, Sicong Wang, Sajedeh Nasr Esfahani, Zida Li, Jonathon M. Muncie, Johnathon N. Lakins, Valerie M. Weaver, Deborah L. Gumucio, Jianping Fu
Article
Cell Biology
Ryan F. Townshend, Yue Shao, Sicong Wang, Chari L. Cortez, Sajedeh Nasr Esfahani, Jason R. Spence, K. Sue O'Shea, Jianping Fu, Deborah L. Gumucio, Kenichiro Taniguchi
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Review
Chemistry, Physical
Jianping Fu, Aryeh Warmflash, Matthias P. Lutolf
Summary: This article highlights the importance of stem-cell-derived embryo models in advancing our understanding of mammalian embryology, emphasizing the significance of simulating mouse and human development in current models and utilizing bioengineering tools.
Article
Biochemical Research Methods
Yi Zheng, Yue Shao, Jianping Fu
Summary: This study presents a microfluidic device for modeling human embryo development, derived from human pluripotent stem cells. The resulting structures exhibit molecular and cellular features resembling early post-implantation human embryo development. The microfluidic model is compatible with high-throughput studies and various analytical techniques.
Article
Multidisciplinary Sciences
Dennis W. Zhou, Marc A. Fernandez-Yaguee, Elijah N. Holland, Andres F. Garcia, Nicolas S. Castro, Eric B. O'Neill, Jeroen Eyckmans, Christopher S. Chen, Jianping Fu, David D. Schlaepfer, Andres J. Garcia
Summary: The coupling of traction force and FAK signaling coordinates cell migration and tissue-scale forces to promote microtissue repair. This process relies on actomyosin contractility, talin-FAK binding, and vinculin to convert forces into FAK signaling events regulating cell migration and tissue repair.
NATURE COMMUNICATIONS
(2021)
Review
Cell & Tissue Engineering
Yue Shao, Jianping Fu
Summary: This article discusses the conceptual and technological frameworks for developing high-fidelity embryoids and organoids that display tissue and organ-level phenotypes and functions. By reconstructing multiscale structural orders and engineering the cellular organization and microenvironment, next-generation, high-fidelity embryoids and organoids can be achieved.
Review
Developmental Biology
Aidan H. Terhune, Jeyoon Bok, Shiyu Sun, Jianping Fu
Summary: The development of a zygote into an embryo is a miraculous process in nature, but scientists have struggled to understand the mechanisms in human embryos due to ethical and practical limitations. However, pluripotent stem cells have the ability to mimic specific embryonic events, allowing the creation of in vitro models that recapitulate different stages of early embryo development.
Article
Chemistry, Multidisciplinary
Yen-Yu Hsu, Agnes M. Resto Irizarry, Jianping Fu, Allen P. Liu
Summary: Plasma membrane tension acts as a regulator of cellular activities, but current measurement techniques are limited in their ability to investigate membrane biophysics and the role of tension. In this study, a new optical reporter for membrane tension is developed using a modified E. coli mechanosensitive channel and circularly permuted GFP, which allows measurement of membrane tension based on fluorescence intensity changes.
Article
Chemistry, Multidisciplinary
Feiyu Yang, Pengcheng Chen, Han Jiang, Tianfa Xie, Yue Shao, Deok-Ho Kim, Bo Li, Yubing Sun
Summary: Strain gradients play a crucial role in directional cell migration. This study develops a programmable uniaxial cell stretch device to create controllable strain gradients and demonstrates that these gradients are sufficient to drive over 60% of rat embryonic fibroblasts towards the lower strain side. Focal adhesion analysis and a motor-clutch model suggest that strain-introduced traction force and integrin fibronectin pairs' catch-release dynamics are responsible for such directional migration.
Article
Multidisciplinary Sciences
Feng Lin, Xia Li, Shiyu Sun, Zhongyi Li, Chenglin Lv, Jianbo Bai, Lin Song, Yizhao Han, Bo Li, Jianping Fu, Yue Shao
Summary: Researchers have developed an efficient biomaterial system that can generate gut spheroids from human pluripotent stem cells through mechanically enhanced tissue morphogenesis. The study also reveals the mechanisms behind gut spheroid formation, including the effects of cell multilayering and crowding, and proposes a mechanism for morphogenetic transitions.
NATURE COMMUNICATIONS
(2023)
Article
Materials Science, Multidisciplinary
Yi Yan, Yue Shao, Xi-Qiao Feng, Zi-Chen Deng
Summary: This paper proposes a nanofibril network model to analyze the deformation, damage, and fracture processes of biological silks. The model reveals how molecular unfolding affects the mechanical behaviors of biological silks and explores how the nanofibrous structure enhances the toughness of biological silks.
JOURNAL OF THE MECHANICS AND PHYSICS OF SOLIDS
(2023)