Article
Biophysics
Yaqian Chen, Jiaqi Ma, Xuechi Yin, Ziai Deng, Xiaojing Liu, Di Yang, Lei Zhao, Jing Sun, Jianlong Wang, Daohong Zhang
Summary: An immersible amplification dip-stick immunoassay (DSIA) using multifunctional nanocomposites was developed for the sensitive detection of Salmonella typhimurium (S. typhi) and Escherichia coli O157:H7 (E. coli O157:H7). The reporter molecule, CoFe2O4 (CFO), possessed superior catalase-like activity and magnetic properties. It enabled a simple and highly sensitive detection with a dual-visible mode. The DSIA achieved matrix-interference-free detection with a dynamic range of 102-108 CFU/mL and a low assay limit of 102 CFU/mL, and showed reasonable recovery rates for contamination monitoring in milk and beef samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Food Science & Technology
Jutanat Srisamran, Edward R. Atwill, Rungtip Chuanchuen, Saharuetai Jeamsripong
Summary: Produce-associated food-borne outbreaks are becoming increasingly important globally. This study investigated the contamination of retail produce from fresh markets and supermarkets in Bangkok with indicator bacteria and pathogens such as Salmonella, Shigella, E. coli O157:H7, and L. monocytogenes. The study found high concentrations of indicator bacteria, higher in produce from fresh markets and produced using conventional methods. The prevalence of Salmonella contamination was linked to the rainy season, conventional agriculture, sweet basil, and certain storage practices.
FOOD QUALITY AND SAFETY
(2022)
Article
Food Science & Technology
Jutanat Srisamran, Edward R. Atwill, Rungtip Chuanchuen, Saharuetai Jeamsripong
Summary: This study investigated bacterial and pathogen contamination in vegetables from markets and supermarkets in Bangkok, identifying predictors associated with contamination. It highlights the importance of monitoring and surveillance for pathogen contamination in produce to enhance food safety.
FOOD QUALITY AND SAFETY
(2022)
Article
Microbiology
Su-Hyeon Kim, Damilare Emmanuel Adeyemi, Mi-Kyung Park
Summary: KFS-EC3 is a polyvalent and lytic phage that can efficiently infect various bacteria, making it suitable for pathogen control in foods.
Article
Food Science & Technology
Martha Sanchez-Tamayo, Blanca Ruiz-Llacsahuanga, Rawane Raad, William Kerr, Faith Critzer
Summary: This study evaluated the efficacy of incorporating natural antimicrobials into carnauba wax and applying it to apples. The results showed that carnauba wax containing 2% essential oils was the most effective treatment for inactivating bacterial foodborne pathogens on apples.
Article
Agriculture, Dairy & Animal Science
Tao Yang, Zhenghui Wang, Yang Song, Xinyan Yang, Sihan Chen, Shiqian Fu, Xue Qin, Wei Zhang, Chaoxin Man, Yujun Jiang
Summary: A smartphone-based colorimetric aptasensor was developed for the detection of E. coli O157:H7 in milk, showing good reproducibility with no cross-reaction for other bacteria. The sensor could detect a concentration of 8.43 x 10^3 cfu/mL of E. coli O157:H7 in pure culture and 5.24 x 10^2 cfu/mL in artificially contaminated milk after 1 hour of incubation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Zhihua Li, Xue Zhang, Haishan Qi, Xiaowei Huang, Jiyong Shi, Xiaobo Zou
Summary: A renewable electrochemical biosensor based on mussel-inspired adhesive protein was developed for the detection of Escherichia coli O157:H7. The biosensor showed excellent repeatability and sensitivity, with a wide linear range and a low detection limit. It demonstrated good performance in detecting E. coli O157:H7 in seafood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Food Science & Technology
Jong-Seong Lim, Jae-Won Ha
Summary: This study found that acid-adapted E. coli O157:H7 exhibited higher X-ray resistance compared to non-acid-adapted cells in apple juice, while acid-adapted and non-acid-adapted Salmonella Typhimurium cells showed similar resistance. The quality attributes of apple juice were not significantly affected by X-ray irradiation, suggesting the importance of considering inactivation of acid-adapted E. coli O157:H7 cells in the application of X-ray sterilization in apple juice processing.
Article
Engineering, Chemical
Gamze Kocer Alasalvar, Nene Meltem Keklik
Summary: The study modeled the survival of L. monocytogenes and changes in quality attributes of sliced mushrooms exposed to PUV light using mathematical models. It was found that the Weibull model better described the inactivation of L. monocytogenes, while zero-order kinetics slightly outperformed first-order kinetics in explaining the changes in quality attributes.
JOURNAL OF FOOD PROCESS ENGINEERING
(2021)
Review
Food Science & Technology
Onay B. Dogan, Makenzie G. Flach, Markus F. Miller, Mindy M. Brashears
Summary: Recently, there have been multiple reports linking leafy green outbreaks to nearby cattle operations. This scoping review aims to gather data on the transmission mechanisms and the association between livestock and produce contamination. Results showed that proximity to livestock might be a risk factor, but there is a lack of quantitative data on the different pathways of contamination.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Article
Biotechnology & Applied Microbiology
Eun-Jung Lee, Soo-Hwan Kim, Sang-Hyun Park
Summary: The objective of this study was to evaluate the survival and cell injury of Salmonella Typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes in apple puree after high hydrostatic pressure (HHP) treatment. The results showed that increasing pressure level and lowering pH of apple puree led to larger reductions in microbial levels. E. coli O157:H7 exhibited higher resistance compared to the other two pathogens.
Article
Chemistry, Analytical
Ziquan Wang, Qian Xu, Siyuan Liu, Yingying Liu, Ying Gao, Meng Wang, Ling Zhang, Haiyan Chang, Qiang Wei, Zhiwei Sui
Summary: A rapid and quantitative method for multiplexed detection of Salmonella, E. coli O157:H7, and S. flexneri in ground beef was developed using a sample pretreatment protocol and flow cytometry, enabling analysis within 1 hour without the need for cultural enrichment. The method demonstrated good recovery rates for the three pathogens and had a detection range of 103 to 108 cells/g, with detection limits of 3.1 x 103 cells/g for Salmonella, 2.1 x 103 cells/g for E. coli O157:H7, and 2.3 x 103 cells/g for S. flexneri in ground beef.
Article
Nanoscience & Nanotechnology
Wei-Lun Huang, Chia-Jung Hsu, Jun Hao Wang, Zi-Han Hsu, Kuan-Hung Lu, Wen-Der Wang, Chia-Chi Huang
Summary: This study demonstrates that carbon nanodots can inhibit bacterial growth even without photoactivation, N-doping, and surface charge, showing potential applications in antibacterial wound dressing and food packaging. The nanodots exhibit antibacterial activities against various bacteria and manageable acute toxicity. The study reveals that the characteristics required for carbon nanodots to be antibacterial are not fully understood, and the biological pathways leading to such activities are more diverse than previously thought.
ACS APPLIED NANO MATERIALS
(2023)
Article
Food Science & Technology
Berta Polanco-Estibalez, Rodrigo Garcia-Santa-Cruz, Rui P. Queiros, Vinicio Serment-Moreno, Mario Gonzalez-Angulo, Carole Tonello-Samson, Maria D. Rivero-Perez
Summary: Cold brew coffee (CBC) is popular for its unique sensory experience, but improper storage can pose a risk for bacterial pathogens. High-Pressure Processing (HPP) is a nonthermal technology that can improve the safety of CBC while maintaining its quality. This study shows that HPP can effectively control pathogens and spoilage microorganisms in CBC while preserving its quality attributes.
Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Food Science & Technology
Xinang Cao, Runze Huang, Haiqiang Chen
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2017)
Editorial Material
Biochemical Research Methods
Hongyi Wu, Yushu Wang, You Wang, Xinang Cao, Yifan Wu, Zhuofei Meng, Qiang Su, Zhongying Wang, Shuai Yang, Weijian Xu, Shiyi Liu, Pan Cheng, Jianxuan Wu, Md. Rezaul Islam Khan, Lin He, Gang Ma
ACS SYNTHETIC BIOLOGY
(2014)
Article
Food Science & Technology
Xinang Cao, Runze Huang, Haiqiang Chen
JOURNAL OF FOOD SCIENCE
(2019)
Article
Biotechnology & Applied Microbiology
Lihan Huang, Nurul Hawa Ahmad, Vijay Juneja, Erika Stapp-Kamotani, Jose Gabiola, Udit Minocha, Robert Phillips, Marie Hooker, Isabel Walls, Kim Cook, James Lindsay
Summary: This study investigated the growth kinetics of foodborne pathogen B. cereus during the commercial production of liquid egg yolk using PLA(2) treatment. The results showed that B. cytotoxicus NVH391-98 can grow prolifically at 50 degrees C, with estimated minimum, optimum, and maximum temperatures of 16.7-18.5, 47.8-48.1, and 52.1-52.4 degrees C, respectively. The dynamic model was validated with isothermal curves and it was found that holding the temperature below 15 degrees C or above 55 degrees C can effectively prevent the growth of B. cytotoxicus in egg yolk.
Article
Biotechnology & Applied Microbiology
Giacomo Mantegazza, Robin Duncan, Nicolo Telesca, Giorgio Gargari, Susanna Perotti, Patrizia Riso, Simone Guglielmetti
Summary: This study investigates whether salad-associated LAB can survive the gastrointestinal transit (GIT) and contribute to the gut microbiota. The results suggest that salad-associated LAB can survive in the gastrointestinal tract, but their quantity does not significantly alter the composition of the gut microbiota.
Article
Biotechnology & Applied Microbiology
Itsaso Baztarrika, Adrian Salazar-Sanchez, Lorena Laorden, Ilargi Martinez-Ballesteros, Rodrigo Alonso, Irati Martinez-Malaxetxebarria
Summary: The infection mechanisms of Arcobacter are still uncertain. This study found that Arcobacter isolates from food and water sources were able to adhere and invade Caco-2 cells, and carried at least two virulence markers. A. butzleri showed higher virulent activity compared to A. cryaerophilus, while A. lanthieri, A. skirrowii, and A. vitoriensis exhibited even higher adhesion and invasion values. Sewage isolates were more adherent and invasive, and had a higher gene content. The association between cadF and hecA with high adhesion capability was identified for the first time.
Article
Biotechnology & Applied Microbiology
Balaji Sundararaman, Karen Shapiro, Andrea Packham, Lauren E. Camp, Rachel S. Meyer, Beth Shapiro, Richard E. Green
Summary: Pathogenic bacteria, viruses, fungi, and protozoa can cause food and waterborne diseases, and surveillance methods are needed. In this study, CNER method was used to prepare enrichment baits for difficult-to-grow pathogens. This method allows direct sequencing of pathogens from samples.
Article
Biotechnology & Applied Microbiology
Guanghui Zhou, Pengcheng Dong, Xin Luo, Lixian Zhu, Yanwei Mao, Yunge Liu, Yimin Zhang
Summary: Interactions between bacterial species in the formation of dual-species biofilms in the food industry are affected by environmental stresses, such as temperature and pH. This study found that the combination of cold and acid stresses significantly increased biofilm formation, and different temperatures resulted in variations in the presence of specific bacterial species and the secretion of extracellular substances. The findings provide valuable insights for addressing mixed biofilms in food processing under diverse stressful conditions.
Article
Biotechnology & Applied Microbiology
Carmen Sanchez, Sonia Garde, Jose Maria Landete, Javier Calzada, Dave J. Baker, Rhiannon Evans, Arjan Narbad, Melinda J. Mayer, Marta Avila
Summary: This study sequenced and compared the genomes of bacteriophages active against C. tyrobutyricum, identifying novel species and phage proteins. A phage showed high lytic activity against C. tyrobutyricum cells and remained stable under different environmental conditions. Additionally, engineered strains successfully delivered endolysin and fluorescent protein to dairy products without affecting their technological properties.
Article
Biotechnology & Applied Microbiology
Danai Etter, Michael Biggel, Mariella Greutmann, Nicole Cernela, Sophia Johler
Summary: Bacillus cytotoxicus is a thermotolerant bacterium that is linked to rare but sometimes fatal cases of diarrheal disease. It is mostly found in dehydrated foods containing potato starch or insects and may be missed in routine screening.
Article
Biotechnology & Applied Microbiology
Tong Jin, Xiangjun Zhan, Liuxin Pang, Bo Peng, Xinpeng Zhang, Wenxiu Zhu, Baowei Yang, Xiaodong Xia
Summary: The study found that CpxAR TCS is an important regulatory mechanism for the virulence of C. sakazakii. Mutants showed weakened adhesion, invasion, and survival ability, and exhibited attenuated virulence in animal models. In addition, CpxAR TCS is involved in regulating the expression of genes associated with bacterial virulence and cellular invasion.
Article
Biotechnology & Applied Microbiology
Soo-Hwan Kim, Sang-Hyun Park, Jun-Bae Ahn, Dong-Hyun Kang
Summary: In this study, a semi continuous SHS system was developed to inactivate foodborne pathogens on radish seed surfaces and enhance the seeds' salinity tolerance. The results showed that higher temperature and more treatments led to a significant reduction in microbial count on the seed surface.
Article
Biotechnology & Applied Microbiology
Yujun Zhai, Weina Tian, Kewei Chen, Linshu Lan, Jianquan Kan, Hui Shi
Summary: This research investigates the role of flagella-related genes in the adhesion of E. coli O157:H7 and the effects of certain cations on bacterial adhesion and motility. The results demonstrate that flagellar-mediated motility is critical in the early stage of adhesion, and the presence of Fe2+, Ca2+, and Mn2+ affects the adhesion and motility of wildtype strains but not flagellar function-impaired strains. These findings provide new insights into the mechanisms of bacterial adhesion and have implications for the development of anti-adhesion agents.
Article
Biotechnology & Applied Microbiology
Mariana Marggraf, Andrea Barac, Jens Andre Hammerl, Stefan Hertwig
Summary: Isolating Yersinia enterocolitica from food is challenging due to overgrowth by other microflora and the lack of a selective medium. The authors improved the standard isolation procedure, resulting in a more sensitive method, reduced background bacteria, and time savings.
Article
Biotechnology & Applied Microbiology
Rafael D. Chaves, Samuel H. Kumazawa, Amin Mousavi Khaneghah, Veronica O. Alvarenga, Humberto M. Hungaro, Anderson S. Sant'Ana
Summary: This study investigated the susceptibility to sanitizers and biofilm-forming ability of 43 Salmonella enterica and Listeria monocytogenes strains on stainless steel surfaces. The results showed variations in susceptibility to sanitizers and biofilm resistance among different strains of the pathogens, with chlorine compounds being more effective in inactivating planktonic cells and biofilms.
Article
Biotechnology & Applied Microbiology
So Yeong Mun, Wooje Lee, Soo-Young Lee, Ji Yoon Chang, Hae Choon Chang
Summary: This study reveals that lactobacilli with a strong defense system dominate the final stage of kimchi fermentation and proposes a model for the succession mechanism of kimchi lactobacilli.
