4.7 Article

Characteristics of albumin and globulin from coconut meat and their role in emulsion stability without and with proteolysis

Journal

FOOD HYDROCOLLOIDS
Volume 69, Issue -, Pages 220-228

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2017.02.006

Keywords

Oil-in-water emulsion; Coconut proteins; Protein pattern; Emulsion stability; Coalescence index; Flocculation factor

Funding

  1. Thailand's Education Hub for Southern Region of ASEAN Countries (TEH-AC) scholarship
  2. Halal Institute Prince of Songkla University [ARG02H55]

Ask authors/readers for more resources

Albumin and globulin were fractionated from defatted coconut meat. Characteristics and emulsifying properties of these protein fractions were comparatively studied. Both fractions had protein with MW of 55 kDa as predominant and glutamine/glutamic acid were the major amino acids. However, differences in the protein pattern and amino acid composition were observed between two fractions. Higher average hydrophobicity was found in globulin fraction, compared with albumin. Additionally, globulin fraction was more hydrolyzed by Alcalase, in comparison with albumin. Coconut milk oil-in-water model emulsion was prepared using albumin and globulin protein fractions and stability of these emulsions was evaluated. Oil droplets with larger size caused by coalescence along with higher polydispersity were observed in albumin stabilized emulsion after 24 h of storage time. Conversely, globulin stabilized emulsion showed smaller oil droplet with low coalescence index and flocculation factor. Thus, emulsion stabilized by globulin fraction was more stable than that containing albumin fraction. However, the higher oil recovery was found in the globulin stabilized emulsion when treated with 1% Alcalase for 90 min, compared with albumin counterpart. This was caused by the higher susceptibility towards hydrolysis of globulin fraction. Therefore, globulin fraction mainly determined coconut milk stability and must be hydrolyzed by protease to release oil for virgin coconut oil production. (C) 2017 Elsevier Ltd. All rights reserved.

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