4.7 Article

Modified QuEChERS combined with ultra high performance liquid chromatography tandem mass spectrometry to determine seven biogenic amines in Chinese traditional condiment soy sauce

Journal

FOOD CHEMISTRY
Volume 229, Issue -, Pages 502-508

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.02.120

Keywords

Biogenic amine; Soy sauce; Ultra high performance liquid chromatography tandem mass spectrometry; Modified QuEChERS

Funding

  1. Technology and Development Center for Petrochemical Pollution Control and Resources Utilization of Guangdong University
  2. Technology Research Center for Petrochemical Resources Clean Utilization of Guangdong Province [201516A01]
  3. Science and Technology Project of Guangdong Province [x2skB2160440]

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A rapid and sensitive UHPLC-MS/MS method followed by modified QuEChERS was developed for the simultaneous determination of seven biogenic amines in soy sauce. Samples were firstly diluted by water and pH values of which were adjusted using ammonia water. Modified QuEChERS method was adopted for the purification and 1, 7-heptyldiamine was used as the internal standard. Analysis of seven biogenic amines was within 4 min. Under the optimized conditions, linear relations were favorable over the selected concentration ranges of 3.0-600 mu g/L with correlation coefficients greater than 0.999. The average accuracy was between 84% and 115%. Intra- and inter- assay precisions for six replicates ranged from 2.2% to 8.8% or below 12%, respectively. The method limits of detection and the method limits of quantitation were 4-8 mu g/kg and 15-30 mu g/kg, respectively. The method was successfully applied to determine the concentrations of seven biogenic amines in soy sauces. (C) 2017 Elsevier Ltd. All rights reserved.

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