Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications

Comparative genomics and transcriptomics of Pichia pastoris

(2016) Kerry R. Love et al.   BMC GENOMICS

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

Endogenous signal peptides in recombinant protein production by Pichia pastoris: From in-silico analysis to fermentation

(2016) Aslan Massahi et al.   JOURNAL OF THEORETICAL BIOLOGY

Multi-omics data driven analysis establishes reference codon biases for synthetic gene design in microbial and mammalian cells

(2016) Kok Siong Ang et al.   METHODS

Engineering of synthetic, stress-responsive yeast promoters

(2016) Arun S. Rajkumar et al.   NUCLEIC ACIDS RESEARCH

Comparison of ADH3 promoter with commonly used promoters for recombinant protein production in Pichia pastoris

(2016) Mert Karaoglan et al.   PROTEIN EXPRESSION AND PURIFICATION

Discovery of a rhamnose utilization pathway and rhamnose-inducible promoters in Pichia pastoris

(2016) Bo Liu et al.   Scientific Reports

Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy

(2016) Yunfeng Zhang et al.   Scientific Reports

DATEL: A Scarless and Sequence-Independent DNA Assembly Method Using Thermostable Exonucleases and Ligase

(2016) Peng Jin et al.   ACS Synthetic Biology

Combinatorial Evolution of Enzymes and Synthetic Pathways Using One-Step PCR

(2016) Peng Jin et al.   ACS Synthetic Biology

Short Synthetic Terminators for Assembly of Transcription Units in Vitro and Stable Chromosomal Integration in Yeast S. cerevisiae

(2016) Murray MacPherson et al.   ACS Synthetic Biology

Enhanced production of leech hyaluronidase by optimizing secretion and cultivation in Pichia pastoris

(2015) Zhen Kang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris

(2015) Verena Puxbaum et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genome-scale metabolic model ofPichia pastoriswith native and humanized glycosylation of recombinant proteins

(2015) Zahra Azimzadeh Irani et al.   BIOTECHNOLOGY AND BIOENGINEERING

Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33

(2015) Ruoyu Mao et al.   BMC MICROBIOLOGY

Editorial: Yeast synthetic biology: new tools to unlock cellular function

(2015)   FEMS YEAST RESEARCH

Expression of enzymes for the usage in food and feed industry with Pichia pastoris

(2015) Sebastian C. Spohner et al.   JOURNAL OF BIOTECHNOLOGY

In-silico determination of Pichia pastoris signal peptides for extracellular recombinant protein production

(2015) Aslan Massahi et al.   JOURNAL OF THEORETICAL BIOLOGY

The development and characterization of synthetic minimal yeast promoters

(2015) Heidi Redden et al.   Nature Communications

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

(2015) Thomas Vogl et al.   ACS Synthetic Biology

Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast

(2015) Kathleen A. Curran et al.   ACS Synthetic Biology

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A New Signal Sequence for Recombinant Protein Secretion in Pichia pastoris

(2014) Nagaraj Govindappa et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Production of the sesquiterpenoid (+)-nootkatone by metabolic engineering of Pichia pastoris

(2014) Tamara Wriessnegger et al.   METABOLIC ENGINEERING

Expression of endoglucanases in Pichia pastoris under control of the GAP promoter

(2014) Anikó Várnai et al.   Microbial Cell Factories

Improvement of catalytic efficiency and thermostability of recombinant Streptomyces griseus trypsin by introducing artificial peptide

(2014) Zhenmin Ling et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Design of synthetic yeast promoters via tuning of nucleosome architecture

(2014) Kathleen A. Curran et al.   Nature Communications

High-yield novel leech hyaluronidase to expedite the preparation of specific hyaluronan oligomers

(2014) Peng Jin et al.   Scientific Reports

Rational Design of a Novel Propeptide for Improving Active Production of Streptomyces griseus Trypsin in Pichia pastoris

(2013) Zhenmin Ling et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris

(2013) Thomas Vogl et al.   CURRENT OPINION IN BIOTECHNOLOGY

Improved secretion of Candida antarctica lipase B with its native signal peptide in Pichia pastoris

(2013) Ashok Kumar Prasanna Vadhana et al.   ENZYME AND MICROBIAL TECHNOLOGY

Use of expression-enhancing terminators in Saccharomyces cerevisiae to increase mRNA half-life and improve gene expression control for metabolic engineering applications

(2013) Kathleen A. Curran et al.   METABOLIC ENGINEERING

Engineering dynamic pathway regulation using stress-response promoters

(2013) Robert H Dahl et al.   NATURE BIOTECHNOLOGY

de novo Design and Synthesis of Candida antarctica Lipase B Gene and α-Factor Leads to High-Level Expression in Pichia pastoris

(2013) Jiang-Ke Yang et al.   PLoS One

Microbials for the production of monoclonal antibodies and antibody fragments

(2013) Oliver Spadiut et al.   TRENDS IN BIOTECHNOLOGY

A Genome-Wide Activity Assessment of Terminator Regions in Saccharomyces cerevisiae Provides a ″Terminatome″ Toolbox

(2013) Mamoru Yamanishi et al.   ACS Synthetic Biology

Synthetic Core Promoters for Pichia pastoris

(2013) Thomas Vogl et al.   ACS Synthetic Biology

Controlling promoter strength and regulation inSaccharomyces cerevisiaeusing synthetic hybrid promoters

(2012) John Blazeck et al.   BIOTECHNOLOGY AND BIOENGINEERING

Endogenous signal peptides efficiently mediate the secretion of recombinant proteins in Pichia pastoris

(2012) Shuli Liang et al.   BIOTECHNOLOGY LETTERS

Functional expression of trypsin from Streptomyces griseus by Pichia pastoris

(2012) Zhenmin Ling et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Comparison of Alpha-Factor Preprosequence and a Classical Mammalian Signal Peptide for Secretion of Recombinant Xylanase xynB from Yeast Pichia pastoris

(2012) Zuyong He   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Rational Diversification of a Promoter Providing Fine-Tuned Expression and Orthogonal Regulation for Synthetic Biology

(2012) Benjamin A. Blount et al.   PLoS One

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Optimization of the production of Aspergillus niger α-glucosidase expressed in Pichia pastoris

(2012) Xu Liu et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Construction of a novel Pichia pastoris strain for production of xanthophylls

(2012) José Araya-Garay et al.   AMB Express

GAPPromoter Library for Fine-Tuning of Gene Expression in Pichia pastoris

(2011) Xiulin Qin et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Hydrophobin signal sequence mediates efficient secretion of recombinant proteins in Pichia pastoris

(2011) Kirsten Kottmeier et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Combinatorial strategy of sorbitol feeding and low-temperature induction leads to high-level production of alkaline β-mannanase in Pichia pastoris

(2011) Taicheng Zhu et al.   ENZYME AND MICROBIAL TECHNOLOGY

SignalP 4.0: discriminating signal peptides from transmembrane regions

(2011) Thomas Nordahl Petersen et al.   NATURE METHODS

FDA approves kallikrein inhibitor to treat hereditary angioedema

(2010) C. A. Thompson   AMERICAN JOURNAL OF HEALTH-SYSTEM PHARMACY

Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement

(2010) Bevan KS Chung et al.   Microbial Cell Factories

mazFas a counter-selectable marker for unmarked genetic modification ofPichia pastoris

(2009) Junjie Yang et al.   FEMS YEAST RESEARCH

An upstream activation sequence controls the expression ofAOX1gene inPichia pastoris

(2009) Yaoji Xuan et al.   FEMS YEAST RESEARCH

Genome sequence of the recombinant protein production host Pichia pastoris

(2009) Kristof De Schutter et al.   NATURE BIOTECHNOLOGY

Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

(2008) Hans Marx et al.   Microbial Cell Factories

Recent advances on the GAP promoter derived expression system of Pichia pastoris

(2008) Ai-Lian Zhang et al.   MOLECULAR BIOLOGY REPORTS

Promoter library designed for fine-tuned gene expression in Pichia pastoris

(2008) Franz S. Hartner et al.   NUCLEIC ACIDS RESEARCH

Secretory expression of interferon-alpha 2b in recombinant Pichia pastoris using three different secretion signals

(2008) Anand Ghosalkar et al.   PROTEIN EXPRESSION AND PURIFICATION

Direct selection ofPichia pastoris expression strains using new G418 resistance vectors

(2008) Joan Lin-Cereghino et al.   YEAST