Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris

In-silico determination of Pichia pastoris signal peptides for extracellular recombinant protein production

(2015) Aslan Massahi et al.   JOURNAL OF THEORETICAL BIOLOGY

Engineering of Protein Folding and Secretion—Strategies to Overcome Bottlenecks for Efficient Production of Recombinant Proteins

(2014) Marizela Delic et al.   ANTIOXIDANTS & REDOX SIGNALING

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Pichia pastorissecretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins

(2014) Andreas Maccani et al.   Biotechnology Journal

Unpacking the Unfolded Protein Response

(2014) Carolyn A. Worby et al.   CELL

Biopharmaceutical discovery and production in yeast

(2014) Michael A Meehl et al.   CURRENT OPINION IN BIOTECHNOLOGY

Golgi compartmentation and identity

(2014) Effrosyni Papanikou et al.   CURRENT OPINION IN CELL BIOLOGY

ER-phagy mediates selective degradation of endoplasmic reticulum independently of the core autophagy machinery

(2014) S. Schuck et al.   JOURNAL OF CELL SCIENCE

High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress and decreases production of human interleukin-10

(2014) Yongjun Zhong et al.   Microbial Cell Factories

Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation and by suppression of vacuolar targeting

(2014) Ivy Fitzgerald et al.   Microbial Cell Factories

Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures

(2014) Ana Vanz et al.   Microbial Cell Factories

GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins

(2014) Leander Meuris et al.   NATURE BIOTECHNOLOGY

Towards improved membrane protein production in Pichia pastoris: General and specific transcriptional response to membrane protein overexpression

(2014) Thomas Vogl et al.   New Biotechnology

Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfolded proteins using chemostat cultures

(2013) Andrew R. Hesketh et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

N-linked protein glycosylation in the ER

(2013) Markus Aebi   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Mutant strains of Pichia pastoris with enhanced secretion of recombinant proteins

(2013) Sasha Larsen et al.   BIOTECHNOLOGY LETTERS

Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing

(2013) Shuli Liang et al.   BMC GENOMICS

Pichia pastoris: protein production host and model organism for biomedical research

(2013) Brigitte Gasser et al.   Future Microbiology

The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris

(2013) Geoff P. Lin-Cereghino et al.   GENE

Production of sialylated O-linked glycans in Pichia pastoris

(2013) S. R. Hamilton et al.   GLYCOBIOLOGY

O-linked glycosylation analysis of recombinant human granulocyte colony-stimulating factor produced in glycoengineered Pichia pastoris by liquid chromatography and mass spectrometry

(2013) Bing Gong et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Disruption of YPS1 and PEP4 genes reduces proteolytic degradation of secreted HSA/PTH in Pichia pastoris GS115

(2013) Min Wu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Glycosylation characterization of recombinant human erythropoietin produced in glycoengineeredPichia pastorisby mass spectrometry

(2013) Bing Gong et al.   JOURNAL OF MASS SPECTROMETRY

Quantitative iTRAQ LC–MS/MS proteomics reveals the cellular response to heterologous protein overexpression and the regulation of HAC1 in Pichia pastoris

(2013) Xiao-qiong Lin et al.   Journal of Proteomics

Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions

(2013) Marc Carnicer et al.   Microbial Cell Factories

Sec16 influences transitional ER sites by regulating rather than organizing COPII

(2013) Nike Bharucha et al.   MOLECULAR BIOLOGY OF THE CELL

Metabolic flux analysis of recombinant Pichia pastoris growing on different glycerol/methanol mixtures by iterative fitting of NMR-derived 13C-labelling data from proteinogenic amino acids

(2013) Joel Jordà et al.   New Biotechnology

Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site

(2013) Song Yang et al.   PLoS One

Characterization of the Pichia pastoris Protein-O-mannosyltransferase Gene Family

(2013) Juergen H. Nett et al.   PLoS One

PMT1 gene plays a major role in O-mannosylation of insulin precursor in Pichia pastoris

(2013) Nagaraj Govindappa et al.   PROTEIN EXPRESSION AND PURIFICATION

The structure of the COPII transport-vesicle coat assembled on membranes

(2013) Giulia Zanetti et al.   eLife

Intracellular interactome of secreted antibody Fab fragment in Pichia pastoris reveals its routes of secretion and degradation

(2012) Martin Pfeffer et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Improvement of N-glycan site occupancy of therapeutic glycoproteins produced in Pichia pastoris

(2012) Byung-Kwon Choi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

IgG-Fc glycoengineering in non-mammalian expression hosts

(2012) Andreas Loos et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Computational codon optimization of synthetic gene for protein expression

(2012) Bevan Chung et al.   BMC Systems Biology

Oxidative protein folding and unfolded protein response elicit differing redox regulation in endoplasmic reticulum and cytosol of yeast

(2012) Marizela Delic et al.   FREE RADICAL BIOLOGY AND MEDICINE

Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes

(2012) Ana Leticia Vanz et al.   Microbial Cell Factories

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Sec12 Binds to Sec16 at Transitional ER Sites

(2012) Elisabeth A. Montegna et al.   PLoS One

Systematic Single-Cell Analysis of Pichia pastoris Reveals Secretory Capacity Limits Productivity

(2012) Kerry Routenberg Love et al.   PLoS One

Optimization of the production of Aspergillus niger α-glucosidase expressed in Pichia pastoris

(2012) Xu Liu et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Secreted production of collagen-inspired gel-forming polymers with high thermal stability in Pichia pastoris

(2011) Catarina I.F. Silva et al.   BIOTECHNOLOGY AND BIOENGINEERING

Single-chain antibody fragment production in Pichia pastoris: Benefits of prolonged pre-induction glycerol feeding

(2011) Narendar K. Khatri et al.   Biotechnology Journal

Optimization of a glycoengineered Pichia pastoris cultivation process for commercial antibody production

(2011) Jianxin Ye et al.   BIOTECHNOLOGY PROGRESS

Protein folding and quality control in the endoplasmic reticulum: Recent lessons from yeast and mammalian cell systems

(2011) Jeffrey L Brodsky et al.   CURRENT OPINION IN CELL BIOLOGY

Activation of the unfolded protein response inPichia pastorisrequires splicing of aHAC1mRNA intron and retention of the C-terminal tail of Hac1p

(2011) Graham Whyteside et al.   FEBS LETTERS

Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedure

(2011) Martin Pfeffer et al.   Microbial Cell Factories

Native-State Stability Determines the Extent of Degradation Relative to Secretion of Protein Variants from Pichia pastoris

(2011) Graham Whyteside et al.   PLoS One

The response to unfolded protein is involved in osmotolerance of Pichia pastoris

(2010) Martin Dragosits et al.   BMC GENOMICS

A multi-level study of recombinant Pichia pastoris in different oxygen conditions

(2010) Kristin Baumann et al.   BMC Systems Biology

Monitoring intracellular redox conditions in the endoplasmic reticulum of living yeasts

(2010) Marizela Delic et al.   FEMS MICROBIOLOGY LETTERS

COPII-mediated vesicle formation at a glance

(2010) D. Jensen et al.   JOURNAL OF CELL SCIENCE

Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

(2010) Pieter P Jacobs et al.   Microbial Cell Factories

The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

(2010) Mouna Guerfal et al.   Microbial Cell Factories

Oxidative Protein Folding in the Secretory Pathway and Redox Signaling Across Compartments and Cells

(2010) Éva Margittai et al.   TRAFFIC

Protein Disulfide Isomerase: A Critical Evaluation of Its Function in Disulfide Bond Formation

(2009) Feras Hatahet et al.   ANTIOXIDANTS & REDOX SIGNALING

Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies inSaccharomyces cerevisiae

(2009) J. Andy Rakestraw et al.   BIOTECHNOLOGY AND BIOENGINEERING

Genome-scale analysis of library sorting (GALibSo): Isolation of secretion enhancing factors for recombinant protein production inPichia pastoris

(2009) Gerhard Stadlmayr et al.   BIOTECHNOLOGY AND BIOENGINEERING

Directed gene copy number amplification inPichia pastorisby vector integration into the ribosomal DNA locus

(2009) Hans Marx et al.   FEMS YEAST RESEARCH

Mechanism and components of endoplasmic reticulum-associated degradation

(2009) J. Hoseki et al.   JOURNAL OF BIOCHEMISTRY

Stress-sensing mechanisms in the unfolded protein response: similarities and differences between yeast and mammals

(2009) K. Kohno   JOURNAL OF BIOCHEMISTRY

The Effect of Temperature on the Proteome of RecombinantPichia pastoris

(2009) Martin Dragosits et al.   JOURNAL OF PROTEOME RESEARCH

Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays

(2008) Alexandra Graf et al.   BMC GENOMICS

One step at a time: endoplasmic reticulum-associated degradation

(2008) Shruthi S. Vembar et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY