de novo Design and Synthesis of Candida antarctica Lipase B Gene and α-Factor Leads to High-Level Expression in Pichia pastoris

A Simple and Accurate Two-Step Long DNA Sequences Synthesis Strategy to Improve Heterologous Gene Expression in Pichia

(2012) Jiang-Ke Yang et al.   PLoS One

Constitutive expression of Botrytis aclada laccase in Pichia pastoris

(2012) Roman Kittl et al.   Bioengineered

Continuous biodiesel production using in situ glycerol separation by membrane bioreactor system

(2011) Myung Joo Ko et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Effect of codon optimization on the expression of Trichoderma reesei endoglucanase 1 in Pichia pastoris

(2011) Gunseli Bayram Akcapinar et al.   BIOTECHNOLOGY PROGRESS

Engineering the Expression and Biochemical Characteristics of RecombinantCandida rugosaLIP2 Lipase by Removing the Additional N-Terminal Peptide and Regional Codon Optimization

(2011) Shu-Wei Chang et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Improving recombinant eukaryotic membrane protein yields inPichia pastoris: The importance of codon optimization and clone selection

(2011) Fredrik Öberg et al.   MOLECULAR MEMBRANE BIOLOGY

Parallel on-chip gene synthesis and application to optimization of protein expression

(2011) Jiayuan Quan et al.   NATURE BIOTECHNOLOGY

Recombinant Candida rugosa lipase 2 from Pichia pastoris: Immobilization and use as biocatalyst in a stereoselective reaction

(2010) M. Dolors Benaiges et al.   BIOTECHNOLOGY PROGRESS

Codon optimization of human factor VIII cDNAs leads to high-level expression

(2010) N. J. Ward et al.   BLOOD

Codon optimization through a two-step gene synthesis leads to a high-level expression of Aspergillus niger lip2 gene in Pichia pastoris

(2010) Jiangke Yang et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Residue-specific global fluorination of Candida antarctica lipase B in Pichia pastoris

(2010) Nediljko Budisa et al.   Molecular BioSystems

Aspergillus nigerlipase: Heterologous expression inPichia pastoris, molecular modeling prediction and the importance of the hinge domains at both sides of the lid domain to interfacial activation

(2009) Zhengyu Shu et al.   BIOTECHNOLOGY PROGRESS

Lipase-Catalyzed Highly Enantioselective Kinetic Resolution of Boron-Containing Chiral Alcohols

(2009) Leandro H. Andrade et al.   ORGANIC LETTERS

Cloning and Expression of Pseudomonas fluorescens 26-2 Lipase Gene in Pichia pastoris and Characterizing for Transesterification

(2008) Jiangke Yang et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Improvement of a Candida antarctica lipase B-displaying yeast whole-cell biocatalyst and its application to the polyester synthesis reaction

(2008) Takanori Tanino et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Expression of Candida antarctica lipase B in Pichia pastoris and various Escherichia coli systems

(2008) Marianne Wittrup Larsen et al.   PROTEIN EXPRESSION AND PURIFICATION