Review
Immunology
Kayla R. Wilson, Elise Gressier, Malcolm J. McConville, Sammy Bedoui
Summary: The respiratory tract serves as a pathway for viruses and bacteria to enter the human body, and dendritic cells play a critical role in protecting against these invaders. This review discusses the roles of different subsets of dendritic cells in respiratory infections and explores how microbial metabolites affect the development and activation of these cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Hilal Bashir, Sanpreet Singh, Raghwendra Pratap Singh, Javed N. Agrewala, Rashmi Kumar
Summary: Age-related loss of immune tolerance leads to autoimmune and inflammatory diseases. Aging and gut dysbiosis disrupt the tolerance of dendritic cells, which are responsible for maintaining immune tolerance. Our study reveals that gut dysbiosis in both aged and young mice leads to the loss of dendritic cell tolerance, highlighting the importance of gut microbiota in immune responses.
Article
Biochemistry & Molecular Biology
Xianmei Liu, Peng Yu, Yujun Xu, Yun Wang, Jin Chen, Fuzhou Tang, Zuquan Hu, Jing Zhou, Lina Liu, Wei Qiu, Yuannong Ye, Yi Jia, Weijuan Yao, Jinhua Long, Zhu Zeng
Summary: Dendritic cells (DCs) can mediate immune responses or immune tolerance depending on their immunophenotype and functional status. Metformin can induce tolerance in DCs by reprogramming their metabolic patterns, thereby exerting anti-inflammatory effects.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Food Science & Technology
Diogo Carregosa, Catarina Pinto, Maria Angeles Avila-Galvez, Paulo Bastos, David Berry, Claudia Nunes Santos
Summary: Numerous epidemiological studies have confirmed the health benefits of consuming fruits, vegetables, and beverages rich in (poly)phenols, especially in preventing and treating cardiovascular and neurological diseases. However, the role of phenolic acids has been overlooked in previous research. This review focuses on two main aspects of low molecular weight phenolic metabolites: the microbiota responsible for their production and their analysis and pharmacokinetics in the human circulation. The aim is to advance scientific understanding of the ubiquitous roles of low molecular weight phenolic metabolites in physiologically relevant concentrations and (patho)physiologically relevant conditions in humans.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Gastroenterology & Hepatology
Katherine M. Kennedy, Andreas Plagemann, Julia Sommer, Marie Hofmann, Wolfgang Henrich, Jon F. R. Barrett, Michael G. Surette, Stephanie Atkinson, Thorsten Braun, Deborah M. Sloboda
Summary: Maternal adaptations to pregnancy are affected by high pre-pregnancy BMI (pBMI) and excess gestational weight gain (GWG). This study suggests that the gut microbiome may play a role in these adaptations and that previous pregnancies can have persistent impacts on maternal adaptations. It also highlights the influence of parity on the effect of maternal GWG on infant gut microbiome.
Article
Pharmacology & Pharmacy
Yuyan Gu, Yaxin Zhang, Mei Li, Zhiyong Huang, Jing Jiang, Yihao Chen, Junqi Chen, Yuhua Jia, Lihua Zhang, Fenghua Zhou
Summary: Ferulic acid (FA) can significantly alleviate atherosclerotic injury in mice by modulating lipid metabolism and gut microbiota. This improvement may be partly achieved through the AMPK alpha/SREBP1/ACC1 pathway.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Marine & Freshwater Biology
Wenting Lin, Yingjun Qin, Xinying Wang, Mingluo Du, Yukai Wang, Xiaohui Chen, Yuan Ren
Summary: This study investigated the effects of benzodiazepine drugs on the gut-liver axis of zebrafish, and found that the exposure to these drugs led to imbalance of gut microbiota and metabolic disorder, as well as oxidative stress in the liver. The metabolomics analysis also revealed significant alterations in amino acid biosynthesis and other metabolic pathways in the liver. These findings provide valuable data for ecological and environmental risk assessments.
AQUATIC TOXICOLOGY
(2023)
Article
Chemistry, Applied
Bowei Zhang, Yingchuan Xu, Huan Lv, Wenwen Pang, Jin Wang, Hui Ma, Shuo Wang
Summary: This study investigated the intestinal pharmacokinetics of resveratrol in mice using HPLC-MS/MS and found that resveratrol and its metabolites were present in significant amounts in the entire intestinal tract and feces, with a peak concentration at 4 hours post-administration. The study also demonstrated the promotion of Lactobacillus reuteri growth by resveratrol-3-O-sulfate during in vitro fermentation and the up-regulation of tight junction and mucin-related proteins mRNA expressions by resveratrol-3-O-sulfate during incubation with Caco-2 cells.
Article
Integrative & Complementary Medicine
Ya-Xin Zhang, Shan-Shan Qu, Li-Hua Zhang, Yu-Yan Gu, Yi-Hao Chen, Zhi-Yong Huang, Meng-Hua Liu, Wei Zou, Jing Jiang, Jun-Qi Chen, Yu-Jue Wang, Feng-Hua Zhou
Summary: The study demonstrates that OPD treatment significantly reduces the development of atherosclerosis, improves lipid levels and liver steatosis, and decreases the relative abundance of Erysipelotrichaceae genera associated with cholesterol metabolism in gut microbiota. OPD may attenuate atherosclerosis by inhibiting mTOR phosphorylation and lipid metabolism signaling pathways, in addition to altering gut microbiota composition and fecal metabolites.
AMERICAN JOURNAL OF CHINESE MEDICINE
(2021)
Review
Immunology
Andreacarola Urso, Alice Prince
Summary: Host and pathogen metabolism play a significant role in the outcome of infection, with immunometabolites influencing the pathogenesis and adaptation of bacteria.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Jia Wei, Wen Dai, Xiongfeng Pan, Yan Zhong, Ningan Xu, Ping Ye, Jie Wang, Jina Li, Fei Yang, Jiayou Luo, Miyang Luo
Summary: This study aimed to evaluate the features and functions of gut microbiota and metabolites in obese children with metabolic syndrome (MS). It found significant differences in 9 microbiota and 26 metabolites between MS children and obese controls. The altered microbiota and metabolites were correlated with clinical indicators of MS. Moreover, the study identified a new biomarker and validated its biological functions. These findings provide new insights into the development of therapeutic strategies for MS.
MICROBIOLOGY SPECTRUM
(2023)
Article
Pharmacology & Pharmacy
Bo Zhang, Yuzhou Wan, Xuefeng Zhou, Haojun Zhang, Hailing Zhao, Liang Ma, Xi Dong, Meihua Yan, Tingting Zhao, Ping Li
Summary: This study investigated the relationship between serum metabolites and gut microbiota in the progression of diabetic kidney disease (DKD). The results showed significant alterations in serum metabolites and the composition and function of gut microbiota in DKD. The study also identified potential metabolic and microbial targets for the treatment of DKD.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Nutrition & Dietetics
Agata Oledzka, Katarzyna Cichocka, Konrad Wolinski, Matthias F. Melzig, Monika E. Czerwinska
Summary: The study aimed to investigate the targeting effect of Cornus mas fruit extract on pancreatic lipase and alpha-amylase, as well as the changes in metabolites and kinetics during gastrointestinal digestion. The results showed that the extract inhibited the activity of pancreatic lipase and alpha-amylase, and compounds such as loganic acid, kaempferol hexoside, and phenolic acids were found to be potentially bio-accessible.
Review
Biochemistry & Molecular Biology
Julio Plaza-Diaz, Ana Isabel Alvarez-Mercado
Summary: Breast cancer is the most common cancer in women and the second leading cause of death in this group. However, the development of breast cancer is not solely dependent on known risk factors. Bacterial metabolites, such as short-chain fatty acids and secondary bile acids, generated in the gut may be linked to breast cancer development and treatment response. Manipulating the gut microbiota through dietary intervention and identifying metabolites associated with breast cancer could lead to new targets for therapy. Metabolomics is a complementary approach to metagenomics in understanding the molecular biology and oncogenesis of breast cancer. This article reviews recent literature on the influence of bacterial and chemotherapy metabolites in breast cancer patients, as well as the impact of diet.
Article
Critical Care Medicine
Silei Sun, Daosheng Wang, Danfeng Dong, Lili Xu, Mengqi Xie, Yihui Wang, Tongtian Ni, Weisong Jiang, Xiaojuan Zhu, Ning Ning, Qian Sun, Shuyuan Zhao, Mengjiao Li, Peili Chen, Meiling Yu, Jian Li, Erzhen Chen, Bing Zhao, Yibing Peng, Enqiang Mao
Summary: This study used microbiome and untargeted metabolomics to analyze stool samples from sepsis patients and identified microbial and metabolic features that may play important roles in disease outcome. Animal model analysis further validated the findings. These findings could help predict the clinical outcome of sepsis patients and provide a basis for exploring new therapies.
Article
Allergy
Guilhermina M. Carriche, Luis Almeida, Philipp Stuve, Lis Velasquez, Ayesha Dhillon-LaBrooy, Urmi Roy, Marc Lindenberg, Till Strowig, Carlos Plaza-Sirvent, Ingo Schmitz, Matthias Lochner, Anna Katharina Simon, Tim Sparwasser
Summary: The study demonstrates that spermidine can act as a modulator of T-cell differentiation and function, promoting the development of Treg cells. Spermidine affects the differentiation of naive T cells both in vitro and in vivo, exerting beneficial effects on gut immunity.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Immunology
Panagiota Mamareli, Friederike Kruse, Chia-wen Lu, Melanie Guderian, Stefan Floess, Katharina Rox, David S. J. Allan, James R. Carlyle, Mark Broenstrup, Rolf Mueller, Luciana Berod, Tim Sparwasser, Matthias Lochner
Summary: This study demonstrates that targeting the enzyme ACC1 can impair intestinal Th1 and Th17 responses by limiting CD4(+) T-cell expansion and infiltration, suggesting ACC1 as a potential target for modulating T-cell-driven intestinal inflammatory responses. However, inhibition of ACC1 also increases susceptibility to infection with C. rodentium and highlights the importance of ACC1-mediated lipogenesis in ROR gamma t(+) ILC function.
MUCOSAL IMMUNOLOGY
(2021)
Letter
Immunology
Stephanie Haack, Sarah Baiker, Jan Schlegel, Markus Sauer, Tim Sparwasser, Daniela Langenhorst, Niklas Beyersdorf
Summary: Research shows that mouse Th1 cells, similar to human Th1 cells, secrete IFN-gamma in response to stimulation with a superagonistic anti-CD28 monoclonal antibody. Crosslinking of the CD28-SA via FcR and CD40-CD40L interactions significantly enhances IFN-gamma release, highlighting the mouse as a useful model organism for studying immune responses in humans.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Immunology
Luis Almeida, Ayesha Dhillon-LaBrooy, Carla N. Castro, Nigatu Adossa, Guilhermina M. Carriche, Melanie Guderian, Saskia Lippens, Sven Dennerlein, Christina Hesse, Bart N. Lambrecht, Luciana Berod, Leif Schauser, Bruce R. Blazar, Markus Kalesse, Rolf Mueller, Luis F. Moita, Tim Sparwasser
Summary: Linezolid and other ribosomal-targeting antibiotics effectively inhibit T cell activity, disrupt mitochondrial function, reduce oxidative phosphorylation levels, and prevent the occurrence of autoimmune diseases.
Article
Biochemistry & Molecular Biology
Yuksel Korkmaz, Behrus Puladi, Kerstin Galler, Peer W. Kaemmerer, Agnes Schroeder, Lina Goelz, Tim Sparwasser, Wilhelm Bloch, Andreas Friebe, James Deschner
Summary: This study confirmed a decreased expression of sGC alpha(1)- and beta(1)-subunits in cementoclasts under inflammatory conditions, which may lead to increased cementum resorption. Therefore, pharmacological activation of sGC in an NO- and heme-independent manner could be considered as a new treatment strategy to inhibit cementum resorption.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Marc Lindenberg, Luis Almeida, Ayesha Dhillon-LaBrooy, Ekkehard Siegel, Birgitta Henriques-Normark, Tim Sparwasser
Summary: The study demonstrates that Clarithromycin affects the frequency and number of IL-17 producing T helper cells in the lungs of mice infected with macrolide-resistant Streptococcus pneumoniae, as well as impairs the tissue-resident memory T cell response. Mechanistically, Clarithromycin inhibits Th17 cell differentiation through ERK pathway inhibition.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Cell Biology
Luis Eduardo Alves Damasceno, Guilherme Cesar Martelossi Cebinelli, Mariane Font Fernandes, Daniele Carvalho Nascimento, Gabriel Azevedo Publio, Marco Aurelio Ramirez Vinolo, Sergio Costa Oliveira, Tim Sparwasser, Thiago Mattar Cunha, Fernando Queiroz Cunha, Jose Carlos Alves-Filho
Summary: This study reveals an intrinsic role of STING in limiting the pathogenic program of T(H)17 cells, suggesting it as a potential target to control T(H)17 cell-mediated inflammation by inducing IL-10 production and reducing IL-17A expression.
Article
Cell Biology
Francesca Rampoldi, Elisa Donato, Leon Ullrich, Malte Deseke, Anika Janssen, Abdi Demera, Inga Sandrock, Anja Bubke, Anna-Lena Juergens, Maxine Swallow, Tim Sparwasser, Christine Falk, Likai Tan, Andreas Trumpp, Immo Prinz
Summary: Immature autoreactive B cells in healthy individuals can transition into mature B cells through direct interaction with y8 T cells. IL-4 production and cell-to-cell contact with CD30L support B cell maturation and induce gene expression. Depletion of y8 T cells leads to B cells getting stuck in the transitional phase and decreased levels of anti-nuclear autoantibodies.
Article
Immunology
Manfred B. Lutz, Shafaqat Ali, Cindy Audiger, Stella E. Autenrieth, Luciana Berod, Venetia Bigley, Laura Cyran, Marc Dalod, Jan Doerrie, Diana Dudziak, Georgina Florez-Grau, Lucila Giusiano, Gloria J. Godoy, Marion Heuer, Anne B. Krug, Christian H. K. Lehmann, Christian T. Mayer, Shalin H. Naik, Stefanie Scheu, Gerty Schreibelt, Elodie Segura, Kristin Sere, Tim Sparwasser, Jurjen Tel, Huaming Xu, Martin Zenke
Summary: This article is part of a series of guidelines on dendritic cells, providing advanced protocols for the preparation and characterization of murine and human dendritic cells. The protocols cover the preparation and generation of dendritic cells from different cellular sources, including the generation of different subsets such as cDC1, cDC2, and pDC using Flt3L in mice, and the generation of bone marrow-derived dendritic cells using GM-CSF. For human dendritic cells, the protocols focus on the culture of CD34(+) cells on OP9 cell layers to generate different subsets, as well as the generation of dendritic cells from peripheral blood monocytes. The article has been peer-reviewed and approved by the co-authors, making it an essential resource for basic and clinical dendritic cell immunologists.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Bjoern E. Clausen, Lukas Amon, Ronald A. Backer, Luciana Berod, Tobias Bopp, Anna Brand, Sven Burgdorf, Luxia Chen, Meihong Da, Ute Distler, Regine J. Dress, Diana Dudziak, Charles-Antoine Dutertre, Christina Eich, Anna Gabele, Melanie Geiger, Florent Ginhoux, Lucila Giusiano, Gloria J. Godoy, Ahmed E. Hamouda, Lukas Hatscher, Lukas Heger, Gordon F. Heidkamp, Lola C. Hernandez, Lukas Jacobi, Tomasz Kaszubowski, Wan Ting Kong, Christian H. K. Lehmann, Tamara Lopez-Lopez, Karsten Mahnke, Dominik Nitsche, Jorg Renkawitz, Rifat A. Reza, Pablo J. Saez, Laura Schlautmann, Madeleine T. Schmitt, Anna Seichter, Malte Sielaff, Tim Sparwasser, Patrizia Stoitzner, Giorgi Tchitashvili, Stefan Tenzer, Nounagnon R. Tochoedo, Damir Vurnek, Fabian Zink, Thomas Hieronymus
Summary: This article provides a collection of protocols for the functional characterization of mouse and human dendritic cells (DC), including endocytosis and metabolism analysis, transcriptomic and proteomic characterization, migration characterization, and measurement of inflammasome and antigen (cross)-presentation activity. These protocols, written by experienced scientists and peer-reviewed by leading experts, are essential resources for basic and clinical DC immunologists.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Lucia Minarrieta, Gloria J. Godoy, Lis N. Velazquez, Peyman Ghorbani, Tim Sparwasser, Luciana Berod
Summary: The CD11c(+)MHCII(+) compartment in GM-CSF cultures contains two populations with different metabolic profiles: MHCII(low)CD11b(high) cells (GM-Macs) and MHCII(high)CD11b(int) cells (GM-DCs). GM-Macs upregulate iNOS and produce nitric oxide (NO) to inhibit mitochondrial respiration (OXPHOS), while promoting glycolytic metabolism in GM-DCs, which do not express iNOS naturally.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Endocrinology & Metabolism
Julia R. C. Nunes, Tyler K. T. Smith, Peyman Ghorbani, Conor O'Dwyer, Natasha A. Trzaskalski, Habiba Dergham, Ciara Pember, Marisa K. Kilgour, Erin E. Mulvihill, Morgan D. Fullerton
Summary: The combination of thermoneutral housing and consumption of a Western diet does not lead to significant disease progression in mice, but it does induce priming of immune-related and fibrotic pathways.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2023)
Article
Microbiology
Peyman Ghorbani, Sang Yong Kim, Tyler K. T. Smith, Lucia Minarrieta, Victoria Robert-Gostlin, Marisa K. Kilgour, Maja Ilijevska, Irina Alecu, Shayne A. Snider, Kaitlyn D. Margison, Julia R. C. Nunes, Daniel Woo, Ciara Pember, Conor O'Dwyer, Julie Ouellette, Pavel Kotchetkov, Julie St-Pierre, Steffany A. L. Bennett, Baptiste Lacoste, Alexandre Blais, Meera G. Nair, Morgan D. Fullerton
Summary: Type 2 cytokines and phosphatidylcholine play important roles in helminth infection by regulating macrophage immune response and metabolism. Inhibition of phosphatidylcholine metabolism can significantly suppress macrophage activation, alter immune cellular composition, and impair immune response to helminth infection. These findings have important implications for understanding the cellular immune landscape under normal conditions and during intestinal helminth infection.
Article
Immunology
Koen Venken, Tine Decruy, Tim Sparwasser, Dirk Elewaut
Summary: Regulatory T cells (Tregs) and invariant natural killer T (iNKT) cells play a crucial role in controlling autoimmune diseases. The study reveals that Treg depletion enhances iNKT cell responses and leads to severe liver and gut pathology in preclinical models of autoimmunity.
