A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris

Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms

(2022) Jucan Gao et al.   ACS Synthetic Biology

Metabolic engineering of Pichia pastoris for myo-inositol production by dynamic regulation of central metabolism

(2022) Qiquan Zhang et al.   Microbial Cell Factories

Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol

(2022) Rui Hou et al.   Synthetic and Systems Biotechnology

Remodelling metabolism for high-level resveratrol production in Yarrowia lipolytica

(2022) Mengsu Liu et al.   BIORESOURCE TECHNOLOGY

Enhancing methanol biotransformation for overproducing fatty acid derivatives

(2022) Xiangyin Chen et al.   Synthetic and Systems Biotechnology

Recombination machinery engineering for precise genome editing in methylotrophic yeast Ogataea polymorpha

(2021) Jiaoqi Gao et al.   iScience

Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris

(2021) Peng Cai et al.   NUCLEIC ACIDS RESEARCH

Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

(2021) Jucan Gao et al.   Synthetic and Systems Biotechnology

Targeted editing of transcriptional activator MXR1 on the Pichia pastoris genome using CRISPR/Cas9 technology

(2020) Chenglin Hou et al.   YEAST

Metabolic engineering of Pichia pastoris for production of isopentanol (3-Methyl-1-butanol)

(2020) Wiparat Siripong et al.   ENZYME AND MICROBIAL TECHNOLOGY

High efficiency CRISPR/Cas9 genome editing system with an eliminable episomal sgRNA plasmid in Pichia pastoris

(2020) Yankun Yang et al.   ENZYME AND MICROBIAL TECHNOLOGY

Metabolic engineering of Pichia pastoris for malic acid production from methanol

(2020) Feng Guo et al.   BIOTECHNOLOGY AND BIOENGINEERING

Construction of a series of episomal plasmids and their application in the development of an efficient CRISPR/Cas9 system in Pichia pastoris

(2019) Yang Gu et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

CRISPR system in the yeast Saccharomyces cerevisiae and its application in the bioproduction of useful chemicals

(2019) Ryosuke Mitsui et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Methods for Enhancing Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Homology-Directed Repair Efficiency

(2019) Xi-Dian Tang et al.   Frontiers in Genetics

CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

(2019) Qi Liu et al.   Microbial Cell Factories

Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris : A review

(2018) Zhiliang Yang et al.   BIOTECHNOLOGY ADVANCES

Engineering the bioconversion of methane and methanol to fuels and chemicals in native and synthetic methylotrophs

(2018) R Kyle Bennett et al.   CURRENT OPINION IN BIOTECHNOLOGY

Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol

(2018) Yiqi Liu et al.   METABOLIC ENGINEERING

Metabolic engineering of Pichia pastoris

(2018) David A. Peña et al.   METABOLIC ENGINEERING

Reprogramming Yeast Metabolism from Alcoholic Fermentation to Lipogenesis

(2018) Tao Yu et al.   CELL

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

(2017) Astrid Weninger et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals

(2017) Tao Yu et al.   Nature Communications

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications

(2016) Zhen Kang et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Hygromycin-resistance vectors for gene expression inPichia pastoris

(2014) Junjie Yang et al.   YEAST

Increased homologous integration frequency in Yarrowia lipolytica strains defective in non-homologous end-joining

(2013) Anne Kretzschmar et al.   CURRENT GENETICS

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One

Construction of new Pichia pastoris X-33 strains for production of lycopene and β-carotene

(2011) J. M. Araya-Garay et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Direct selection ofPichia pastoris expression strains using new G418 resistance vectors

(2008) Joan Lin-Cereghino et al.   YEAST