Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms

Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9

(2022) Jucan Gao et al.   ACS Synthetic Biology

PCR & Go: A Pre-installed Expression Chassis for Facile Integration of Multi-Gene Biosynthetic Pathways

(2021) Mingming Qi et al.   Frontiers in Bioengineering and Biotechnology

Mechanisms restraining break‐induced replication at two‐ended DNA double‐strand breaks

(2021) Nhung Pham et al.   EMBO JOURNAL

Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris

(2021) Peng Cai et al.   NUCLEIC ACIDS RESEARCH

Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

(2021) Jucan Gao et al.   Synthetic and Systems Biotechnology

Dual roles of yeast Rad51 N-terminal domain in repairing DNA double-strand breaks

(2020) Tai-Ting Woo et al.   NUCLEIC ACIDS RESEARCH

Phosphorylated CtIP bridges DNA to promote annealing of broken ends

(2020) Robin Öz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design

(2019) Xiaoyun Lu et al.   Nature Communications

First Betalain-Producing Bacteria Break the Exclusive Presence of the Pigments in the Plant Kingdom

(2019) Luis Eduardo Contreras-Llano et al.   mBio

Construction of a series of episomal plasmids and their application in the development of an efficient CRISPR/Cas9 system in Pichia pastoris

(2019) Yang Gu et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Methods for Enhancing Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Homology-Directed Repair Efficiency

(2019) Xi-Dian Tang et al.   Frontiers in Genetics

CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

(2019) Qi Liu et al.   Microbial Cell Factories

Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii

(2019) Neil C. Dalvie et al.   ACS Synthetic Biology

Advances in engineering methylotrophic yeast for biosynthesis of valuable chemicals from methanol

(2018) Xingpeng Duan et al.   CHINESE CHEMICAL LETTERS

Metabolic engineering of Pichia pastoris

(2018) David A. Peña et al.   METABOLIC ENGINEERING

Recent advances in metabolic engineering of Saccharomyces cerevisiae : New tools and their applications

(2018) Jiazhang Lian et al.   METABOLIC ENGINEERING

Process development for the continuous production of heterologous proteins by the industrial yeast, Komagataella phaffii

(2018) Ayca Cankorur-Cetinkaya et al.   BIOTECHNOLOGY AND BIOENGINEERING

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

(2017) Astrid Weninger et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose

(2015) William C DeLoache et al.   Nature Chemical Biology

The development and characterization of synthetic minimal yeast promoters

(2015) Heidi Redden et al.   Nature Communications

Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast

(2015) Kathleen A. Curran et al.   ACS Synthetic Biology

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Synthetic Core Promoters for Pichia pastoris

(2013) Thomas Vogl et al.   ACS Synthetic Biology