Modification of Fatty Acid Profile and Oil Contents Using Gene Editing in Oilseed Crops for a Changing Climate

Applications and challenges of harnessing genome editing in oilseed crops

(2023) Papa Rao Vaikuntapu et al.   JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY

MOTHER‐OF‐FT‐AND‐TFL1 regulates the seed oil and protein content in soybean

(2023) Zhandong Cai et al.   NEW PHYTOLOGIST

Improved fatty acid composition of field cress (Lepidium campestre) by CRISPR/Cas9-mediated genome editing

(2023) Sjur Sandgrind et al.   Frontiers in Plant Science

CRISPR/Cas9-Targeted Mutagenesis of BnaFAE1 Genes Confers Low-Erucic Acid in Brassica napus

(2022) Yunhao Liu et al.   Frontiers in Plant Science

Efficient Genetic Transformation of Rice for CRISPR/Cas9 Mediated Genome-Editing and Stable Overexpression Studies: A Case Study on Rice Lipase 1 and Galactinol Synthase Encoding Genes

(2022) Tanika Thakur et al.   Agronomy-Basel

A native promoter–gene fusion created by CRISPR/Cas9‐mediated genomic deletion offers a transgene‐free method to drive oil accumulation in leaves

(2022) Rupam Kumar Bhunia et al.   FEBS LETTERS

Transcriptomic and metabolomics-based analysis of key biological pathways reveals the role of lipid metabolism in response to salt stress in the root system of Brassica napus

(2022) Weichao Wang et al.   PLANT GROWTH REGULATION

Identification of AhFatB genes through genome-wide analysis and knockout of AhFatB reduces the content of saturated fatty acids in peanut (Arichis hypogaea L.)

(2022) Yanyan Tang et al.   PLANT SCIENCE

Functional Differentiation of BnVTE4 Gene Homologous Copies in α-Tocopherol Biosynthesis Revealed by CRISPR/Cas9 Editing

(2022) Haiyan Zhang et al.   Frontiers in Plant Science

CRISPR/Cas9 Based Site-Specific Modification of FAD2 cis-Regulatory Motifs in Peanut (Arachis hypogaea L)

(2022) Anjanasree K. Neelakandan et al.   Frontiers in Genetics

Functional characterization of transcription factor WIN1 genes associated with lipid biosynthesis and stress tolerance in soybean (Glycine max)

(2022) Guiping Cai et al.   ENVIRONMENTAL AND EXPERIMENTAL BOTANY

Enhancing the accumulation of eicosapentaenoic acid and docosahexaenoic acid in transgenic Camelina through the CRISPR‐Cas9 inactivation of the competing FAE1 pathway

(2022) Lihua Han et al.   PLANT BIOTECHNOLOGY JOURNAL

Modulation of GmFAD3 expression alters abiotic stress responses in soybean

(2022) Ajay Kumar Singh et al.   PLANT MOLECULAR BIOLOGY

Generating an oilseed rape mutant with non-abscising floral organs using CRISPR/Cas9 technology

(2022) Jian Wu et al.   PLANT PHYSIOLOGY

RNAi and CRISPR–Cas silencing E3-RING ubiquitin ligase AIP2 enhances soybean seed protein content

(2022) Bo Shen et al.   JOURNAL OF EXPERIMENTAL BOTANY

CRISPR/Cas9 mediated gene-editing of GmHdz4 transcription factor enhances drought tolerance in soybean (Glycine max [L.] Merr.)

(2022) Xuanbo Zhong et al.   Frontiers in Plant Science

Applications and prospects of genome editing in plant fatty acid and triacylglycerol biosynthesis

(2022) Mid-Eum Park et al.   Frontiers in Plant Science

CRISPR/Cas9-Mediated Gene Editing of BnFAD2 and BnFAE1 Modifies Fatty Acid Profiles in Brassica napus

(2022) Jianghua Shi et al.   Genes

Kinase CIPK9 integrates glucose and abscisic acid signaling to regulate seed oil metabolism in rapeseed

(2022) Nan Wang et al.   PLANT PHYSIOLOGY

CRISPR/Cas9-mediated editing of double loci of BnFAD2 increased the seed oleic acid content of rapeseed (Brassica napus L.)

(2022) Han Liu et al.   Frontiers in Plant Science

CRISPR/Cas9-Mediated Mutagenesis of GmFAD2-1A and/or GmFAD2-1B to Create High-Oleic-Acid Soybean

(2022) Mingxue Fu et al.   Agronomy-Basel

Enhancement of Tobacco (Nicotiana tabacum L.) Seed Lipid Content for Biodiesel Production by CRISPR-Cas9-Mediated Knockout of NtAn1

(2021) Yinshuai Tian et al.   Frontiers in Plant Science

Omics and CRISPR-Cas9 Approaches for Molecular Insight, Functional Gene Analysis, and Stress Tolerance Development in Crops

(2021) Muhammad Khuram Razzaq et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

CRISPR/Cas9-Mediated Knockout of GmFATB1 Significantly Reduced the Amount of Saturated Fatty Acids in Soybean Seeds

(2021) Jing Ma et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Optimising glyphosate tolerance in rapeseed (Brassica napus L.) by CRISPR/Cas9-based geminiviral donor DNA replicon system with Csy4-based single-guide RNA processing

(2021) Zhuanrong Wang et al.   JOURNAL OF EXPERIMENTAL BOTANY

CRISPR/Cas9-Induced fad2 and rod1 Mutations Stacked With fae1 Confer High Oleic Acid Seed Oil in Pennycress (Thlaspi arvense L.)

(2021) Brice A. Jarvis et al.   Frontiers in Plant Science

TILLING-by-Sequencing+ Reveals the Role of Novel Fatty Acid Desaturases (GmFAD2-2s) in Increasing Soybean Seed Oleic Acid Content

(2021) Naoufal Lakhssassi et al.   Cells

Based on the whole genome clarified the evolution and expression process of fatty acid desaturase genes in three soybeans

(2021) Bingxue Zhang et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

GmRAV confers ecological adaptation through photoperiod control of flowering time and maturity in soybean

(2021) Yuhe Wang et al.   PLANT PHYSIOLOGY

Increasing Monounsaturated Fatty Acid Contents in Hexaploid Camelina sativa Seed Oil by FAD2 Gene Knockout Using CRISPR-Cas9

(2021) Kyeong-Ryeol Lee et al.   Frontiers in Plant Science

Characterization of SHATTERPROOF Homoeologs and CRISPR-Cas9-Mediated Genome Editing Enhances Pod-Shattering Resistance in Brassica napus L.

(2021) Qamar U. Zaman et al.   CRISPR Journal

Removing the major allergen Bra j I from brown mustard ( Brassica juncea ) by CRISPR/Cas9

(2021) Juvenal Assou et al.   PLANT JOURNAL

Genetic and genomic approaches to address rapid rancidity of rice bran

(2020) Haritha Bollinedi et al.   CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION

Natural variation and CRISPR/Cas9‐mediated mutation in GmPRR37 affect photoperiodic flowering and contribute to regional adaptation of soybean

(2020) Liwei Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Elevating seed oil content in a polyploid crop by induced mutations in SEED FATTY ACID REDUCER genes

(2020) Nirosha L. Karunarathna et al.   PLANT BIOTECHNOLOGY JOURNAL

Gene editing of three BnITPK genes in tetraploid oilseed rape leads to significant reduction of phytic acid in seeds

(2020) Niharika Sashidhar et al.   PLANT BIOTECHNOLOGY JOURNAL

Loss of function of CRT1a (calreticulin) reduces plant susceptibility to Verticillium longisporum in both Arabidopsis thaliana and oilseed rape ( Brassica napus )

(2020) Michael Pröbsting et al.   PLANT BIOTECHNOLOGY JOURNAL

Modifications of fatty acid profile through targeted mutation at BnaFAD2 gene with CRISPR/Cas9-mediated gene editing in Brassica napus

(2020) Huibin Huang et al.   THEORETICAL AND APPLIED GENETICS

CRISPR/Cas-Mediated Genome Editing for the Improvement of Oilseed Crop Productivity

(2020) Udaya Subedi et al.   CRITICAL REVIEWS IN PLANT SCIENCES

Latest Developed Strategies to Minimize the Off-Target Effects in CRISPR-Cas-Mediated Genome Editing

(2020) Muhammad Naeem et al.   Cells

The Potential of Genome Editing for Improving Seed Oil Content and Fatty Acid Composition in Oilseed Crops

(2020) Udaya Subedi et al.   LIPIDS

Plant Unsaturated Fatty Acids: Multiple Roles in Stress Response

(2020) Mei He et al.   Frontiers in Plant Science

High‐oleic acid content, nontransgenic allotetraploid cotton ( Gossypium hirsutum L.) generated by knockout of GhFAD2 genes with CRISPR/Cas9 system

(2020) Yizhen Chen et al.   PLANT BIOTECHNOLOGY JOURNAL

Mutagenesis of FAD2 genes in peanut with CRISPR/Cas9 based gene editing

(2019) Mei Yuan et al.   BMC BIOTECHNOLOGY

FvC5SD overexpression enhances drought tolerance in soybean by reactive oxygen species scavenging and modulating stress-responsive gene expression

(2019) Ling Zhang et al.   PLANT CELL REPORTS

CRISPR/Cas9 editing of three CRUCIFERIN C homoeologues alters the seed protein profile in Camelina sativa

(2019) Wendy J. Lyzenga et al.   BMC PLANT BIOLOGY

Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean

(2019) Phat T. Do et al.   BMC PLANT BIOLOGY

Characteristics of membrane-bound fatty acid desaturase (FAD) genes in Brassica napus L. and their expressions under different cadmium and salinity stresses

(2019) Ling Xu et al.   ENVIRONMENTAL AND EXPERIMENTAL BOTANY

Mutagenesis of GmFT2a and GmFT5a mediated by CRISPR/Cas9 contributes for expanding the regional adaptability of soybean

(2019) Yupeng Cai et al.   PLANT BIOTECHNOLOGY JOURNAL

Effective editing for lysophosphatidic acid acyltransferase 2/5 in allotetraploid rapeseed (Brassica napus L.) using CRISPR-Cas9 system

(2019) Kai Zhang et al.   Biotechnology for Biofuels

Knockout of two Bna MAX 1 homologs by CRISPR /Cas9‐targeted mutagenesis improves plant architecture and increases yield in rapeseed ( Brassica napus L.)

(2019) Ming Zheng et al.   PLANT BIOTECHNOLOGY JOURNAL

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca 2+ signaling pathways in transgenic soybean

(2019) Wei Wei et al.   PLANT JOURNAL

Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.

(2019) Yungu Zhai et al.   PLANT BIOTECHNOLOGY JOURNAL

With a free pass, CRISPR-edited plants reach market in record time

(2018) Emily Waltz   NATURE BIOTECHNOLOGY

Precise editing of CLAVATA genes in Brassica napus L. regulates multilocular silique development

(2018) Yang Yang et al.   PLANT BIOTECHNOLOGY JOURNAL

Mutagenesis of the FAE1 genes significantly changes fatty acid composition in seeds of Camelina sativa

(2018) Mehmet E. Ozseyhan et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Production of high oleic/low linoleic rice by genome editing

(2018) Kiyomi Abe et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

CRISPR/Cas9-mediated genome editing of the fatty acid desaturase 2 gene in Brassica napus

(2018) Ayako Okuzaki et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Physiological and transcriptomic responses of reproductive stage soybean to drought stress

(2018) Congshan Xu et al.   PLANT CELL REPORTS

An APETALA1 ortholog affects plant architecture and seed yield component in oilseed rape (Brassica napus L.)

(2018) Smit Shah et al.   BMC PLANT BIOLOGY

Progress in modification of sunflower oil to expand its industrial value

(2017) Saeed Rauf et al.   JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE

Simultaneous Targeting of Multiple Gene Homeologs to Alter Seed Oil Production in Camelina sativa

(2017) J. A. Aznar-Moreno et al.   PLANT AND CELL PHYSIOLOGY

Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing

(2017) Wen Zhi Jiang et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR-Cas9 Targeted Mutagenesis Leads to Simultaneous Modification of Different Homoeologous Gene Copies in Polyploid Oilseed Rape ( Brassica napus )

(2017) Janina Braatz et al.   PLANT PHYSIOLOGY

Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription

(2017) Ping Xu et al.   Scientific Reports

Transgenic expression of ThIPK2 gene in soybean improves stress tolerance, oleic acid content and seed size

(2012) Miao Liu et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Effect of different environmental stresses on the expression of oleate desaturase genes and fatty acid composition in olive fruit

(2010) M. Luisa Hernández et al.   PHYTOCHEMISTRY