Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean

Simultaneous site-directed mutagenesis of duplicated loci in soybean using a single guide RNA

(2018) Yuhei Kanazashi et al.   PLANT CELL REPORTS

CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula

(2017) Shaun J. Curtin et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9-mediated targeted mutagenesis of GmFT2a delays flowering time in soya bean

(2017) Yupeng Cai et al.   PLANT BIOTECHNOLOGY JOURNAL

Dual-targeting by CRISPR/Cas9 for precise excision of transgenes from rice genome

(2017) Vibha Srivastava et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Heritable Genomic Fragment Deletions and Small Indels in the Putative ENGase Gene Induced by CRISPR/Cas9 in Barley

(2017) Eszter Kapusi et al.   Frontiers in Plant Science

Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans

(2017) Sébastien Mercx et al.   Frontiers in Plant Science

Application of CRISPR/Cas9 in plant biology

(2017) Xuan Liu et al.   Acta Pharmaceutica Sinica B

Direct stacking of sequence-specific nuclease-induced mutations to produce high oleic and low linolenic soybean oil

(2016) Zachary L. Demorest et al.   BMC PLANT BIOLOGY

Efficient targeted mutagenesis in soybean by TALENs and CRISPR/Cas9

(2016) Hongyang Du et al.   JOURNAL OF BIOTECHNOLOGY

Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice

(2016) Rongfang Xu et al.   Journal of Genetics and Genomics

The CRISPR/Cas9 system for plant genome editing and beyond

(2015) Luisa Bortesi et al.   BIOTECHNOLOGY ADVANCES

Targeted genome modifications in soybean with CRISPR/Cas9

(2015) Thomas B Jacobs et al.   BMC BIOTECHNOLOGY

Cas9-Guide RNA Directed Genome Editing in Soybean

(2015) Zhongsen Li et al.   PLANT PHYSIOLOGY

Targeted mutagenesis in soybean using the CRISPR-Cas9 system

(2015) Xianjun Sun et al.   Scientific Reports

CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme

(2015) Jean-Michel Michno et al.   GM Crops & Food-Biotechnology in Agriculture and the Food Chain

CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

(2015) Manuel Stemmer et al.   PLoS One

CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots

(2015) Yupeng Cai et al.   PLoS One

Cut Site Selection by the Two Nuclease Domains of the Cas9 RNA-guided Endonuclease

(2014) Hongfan Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice

(2014) Huanbin Zhou et al.   NUCLEIC ACIDS RESEARCH

Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family

(2014) William Haun et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

(2014) C. Brooks et al.   PLANT PHYSIOLOGY

Efficient genome editing in plants using a CRISPR/Cas system

(2013) Zhengyan Feng et al.   CELL RESEARCH

A novel FAD2-1 A allele in a soybean plant introduction offers an alternate means to produce soybean seed oil with 85% oleic acid content

(2011) Anh-Tung Pham et al.   THEORETICAL AND APPLIED GENETICS

Mutant alleles of FAD2-1A and FAD2-1B combine to produce soybeans with the high oleic acid seed oil trait

(2010) Anh-Tung Pham et al.   BMC PLANT BIOLOGY

Genome sequence of the palaeopolyploid soybean

(2010) Jeremy Schmutz et al.   NATURE

RNAi trigger fragment truncation attenuates soybean FAD2-1 transcript suppression and yields intermediate oil phenotypes

(2010) Nicholas Wagner et al.   PLANT BIOTECHNOLOGY JOURNAL

New sources of soybean seed meal and oil composition traits identified through TILLING

(2009) Emily C Dierking et al.   BMC PLANT BIOLOGY

Two high-oleic-acid soybean mutants, M23 and KK21, have disrupted microsomal omega-6 fatty acid desaturase, encoded by GmFAD2-1a

(2008) Toyoaki Anai et al.   BREEDING SCIENCE

Silencing of GmFAD3 gene by siRNA leads to low α-linolenic acids (18:3) of fad3-mutant phenotype in soybean [Glycine max (Merr.)]

(2008) Teresita Flores et al.   TRANSGENIC RESEARCH