Article
Plant Sciences
Mil Thi Tran, Geon Hui Son, Young Jong Song, Ngan Thi Nguyen, Seonyeong Park, Thanh Vu Thach, Jihae Kim, Yeon Woo Sung, Swati Das, Dibyajyoti Pramanik, Jinsu Lee, Ki-Ho Son, Sang Hee Kim, Tien Van Vu, Jae-Yean Kim
Summary: CRISPR-Cas9-based genome editing was used to edit a tomato gene, resulting in the elimination of the PRD and 8CM domains. The edited tomato plants showed increased tolerance to various stresses, including high temperature, osmotic stress, and Pseudomonas syringae pv. tomato infection.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Sangeeta Banerjee, Ananya Mukherjee, Atreyee Kundu
Summary: The primary goal of plant biotechnologists is to produce a designer crop with added attributes. Genetic engineering allows the displacement of genes among species, and the introduction of foreign genes can create new traits in the host genome. Oilseed mustard varieties have been successfully transformed with genes from a wide range of species, resulting in improved yield and value. However, the genetic transformation of oilseed mustard still faces challenges, and further research is needed to improve the transformation system.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Mayank Chaudhary, Tapan Kumar Mukherjee, Raj Singh, Mahiti Gupta, Soniya Goyal, Paavan Singhal, Rakesh Kumar, Nabin Bhusal, Pooja Sharma
Summary: The CRISPR/Cas9 system is a gene editing tool that can be used in both prokaryotic and eukaryotic cells, allowing for targeted improvements in crop yield, disease resistance, and nutritional value.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Murugesan Chandrasekaran, Thangavelu Boopathi, Manivannan Paramasivan
Summary: CRISPR/Cas9 technology has revolutionized plant biology, especially in dicot crops like tomato, by allowing precise genome editing with minimal off-target mutations, ease of experimental design, higher efficiency, versatility, and cost-effectiveness. This technology has the potential to enhance growth through optimal gene discovery, de novo modification, trait improvement, and biotic/abiotic stress management.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Editorial Material
Plant Sciences
Angeles Aroca, Irene Garcia
Summary: Plant molecular biology has made significant progress in recent years, uncovering multiple pathways and mechanisms underlying plant development and adaptation to different environments. Advances in molecular techniques and gene editing have further enhanced our understanding of plant biology. This research is of great importance in addressing the challenges of increasing global food supply and mitigating the impacts of climate change in the 21st century.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Plant Sciences
Manoj Kumar, Manas Ranjan Prusty, Manish K. Pandey, Prashant Kumar Singh, Abhishek Bohra, Baozhu Guo, Rajeev K. Varshney
Summary: Abiotic stresses, such as drought, salinity, cold, heat, and heavy metals, significantly decrease global agricultural production. Traditional breeding methods and transgenic technology have been used to mitigate these environmental stresses. The discovery of engineered nucleases and the CRISPR/Cas gene-editing tool have revolutionized the management of abiotic stress conditions and offer potential for improving crop tolerance. This review summarizes the latest research on understanding the mechanisms of plant response to abiotic stress and the application of CRISPR/Cas-mediated gene editing in enhancing tolerance to various stresses.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Plant Sciences
Shabir H. Wani, Shruti Anand, Balwant Singh, Abhishek Bohra, Rohit Joshi
Summary: WRKY transcription factors are essential regulators in plants, playing critical roles in response to biotic and abiotic stresses. They modulate various biological processes in plants by regulating signal transduction pathways, response to internal signals, developmental coordination, and adaptation to stress environments. Through interactions with other transcription factors, WRKY TFs form part of signaling webs that regulate diverse processes and plant immune response.
PLANT CELL REPORTS
(2021)
Review
Plant Sciences
Bindu Yadav, Abhimanyu Jogawat, Shambhu Krishan LalNITRATE, Nita Lakra, Sahil Mehta, Nitzan Shabek, Om Prakash Narayan
Summary: Nutrient transporter genes have the potential to improve crop yield by enhancing nutrient uptake and providing tolerance against different biotic and abiotic stresses. Understanding the mechanisms of nutrient transport in crop plants could lead to the development of engineered plant crops with increased yield and improved nutrient quality.
Review
Plant Sciences
Mengxi Wu, Trent R. Northen, Yezhang Ding
Summary: Plants produce specialized metabolites that play crucial roles in environmental interactions and stress adaptation. These compounds have significant agricultural, medicinal, and industrial values. Recent advances in genomics, transcriptomics, metabolomics, proteomics, and computational approaches have made the exploration of plant specialized metabolism more feasible. These advancements hold promise for enhancing plant stress resilience through harnessing the potential of specialized metabolites.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Genetics & Heredity
Amman Khokhar, Muhammad Shahbaz, Muhammad Faisal Maqsood, Usman Zulfiqar, Nargis Naz, Usama Zafar Iqbal, Maheen Sara, Muhammad Aqeel, Noreen Khalid, Ali Noman, Faisal Zulfiqar, Khalid M. Al Syaad, Manal Abdullah AlShaqhaa
Summary: This review provides a comprehensive overview of various genetic modification techniques employed to enhance plant resilience, including strategies such as introducing stress-responsive genes, transcription factors, and regulatory elements to enhance stress signaling pathways. Additionally, the manipulation of hormone signaling pathways, osmoprotectant accumulation, and antioxidant defense mechanisms is discussed, as well as the use of genome editing tools like CRISPR-Cas9 for precise modification of target genes related to stress tolerance. The challenges and future prospects of genetic modification for abiotic stress tolerance are highlighted.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Review
Plant Sciences
Dhanawantari L. Singha, Debajit Das, Yogita N. Sarki, Naimisha Chowdhury, Monica Sharma, Jitendra Maharana, Channakeshavaiah Chikkaputtaiah
Summary: Tissue-specific CRISPR/Cas genome editing is a promising approach for crop improvement, bypassing the issues associated with constitutive genome editing and allowing targeted improvement of crops.
Review
Biotechnology & Applied Microbiology
Mohamed Kouhen, Pedro Garcia-Caparros, Richard M. Twyman, Chedly Abdelly, Henda Mahmoudi, Stefan Schillberg, Ahmed Debez
Summary: Genome editing, especially the CRISPR/Cas9 system, has become an indispensable tool in basic and applied sciences. This paper discusses the recent advances in CRISPR/Cas9 and its applications in plant breeding, focusing on environmental stress tolerance and extremophile plants. The research provides insights into quantitative traits affecting crop yield and stress tolerance, and explores the potential of unexplored genetic resources in ensuring food security under climate change.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Plant Sciences
Vanesa E. Tossi, Leandro J. Martinez Tosar, Leandro E. Laino, Jesica Iannicelli, Jose Javier Regalado, Alejandro Salvio Escandon, Irene Baroli, Humberto Fabio Causin, Sandra Irene Pitta-Alvarez
Summary: Polyploidy is considered a key driving force in evolutionary history and plays a significant role in crop domestication. Research shows that polyploidy can enhance plant tolerance to abiotic and biotic stresses, positively impacting plant growth and yield.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Cell Biology
Yaxin Wang, Naeem Zafar, Qurban Ali, Hakim Manghwar, Guanying Wang, Lu Yu, Xiao Ding, Fang Ding, Ni Hong, Guoping Wang, Shuangxia Jin
Summary: CRISPR/Cas-mediated genome editing is a game-changing tool for rapidly improving desirable traits in crops. It allows for easy applications, high efficiency, and low cost in producing targeted edits, leading to increased quality and yield in a shorter time. This technology holds great potential for creating crop varieties with resistance to biotic and abiotic stresses.
Review
Plant Sciences
Jagesh Kumar Tiwari, Anand Kumar Singh, Tusar Kanti Behera
Summary: The narrow genetic base of tomato presents challenges in breeding, but with the development of CRISPR/Cas9 genome editing, fast and efficient breeding has become possible. CRISPR/Cas9 has been used to edit and characterize various traits in tomato, such as plant architecture, flower characters, fruit quality, disease resistance, and stress tolerance. It has also been used to introduce elite traits from wild relatives to cultivated tomatoes. This mini-review highlights the current research progress in using CRISPR/Cas9 for tomato breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Ecology
Chang Tian, Jingwen Pang, Chongfeng Bu, Shufang Wu, Hao Bai, Yahong Li, Qi Guo, Kadambot H. M. Siddique
Summary: This study compares the microbiomes of lichen and moss biocrust using shotgun metagenomic sequencing and reveals the differences in microbial genes and metabolic pathways involved in carbon and nitrogen cycling.
Article
Agriculture, Multidisciplinary
Miao-miao Zhang, Peng-fei Dang, Yu-ze Li, Xiao-liang Qin, Kadambot H. M. Siddique
Summary: Plastic film mulch systems are widely used in arid areas, and the most suitable tillage method for these systems is still debated. A two-year field experiment was conducted to investigate the impact of three tillage treatments on soil properties, root development, nitrogen utilization, and maize yield. The results showed that plow tillage before plastic film mulch had better soil quality, improved root development, and increased nitrogen transfer and utilization compared to other treatments. As a result, plow tillage significantly increased maize yield and grain quality.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Plant Sciences
Jian Dai, Hailong Gui, Feng Shen, Yuying Liu, Minsong Bai, Jinfeng Yang, Houjun Liu, Peiyu Luo, Xiaori Han, Kadambot H. M. Siddique
Summary: Global awareness of the importance of increasing crop production and reducing environmental issues associated with nitrogen (N) fertilizer has led to a study evaluating the fate of N with manure addition. The findings show that applying manure significantly increases grain yield and improves N recovery efficiency. Therefore, using N, P, and K fertilizers in the soybean season and NPK combined with manure in the maize seasons is a promising fertilization management strategy in Northeast China and similar regions.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Qian Zhang, Wenquan Niu, Yadan Du, Jun Sun, Bingjing Cui, Erxin Zhang, Yanbang Wang, Kadambot H. M. Siddique
Summary: This study found that aerated drip irrigation (ADI) can improve the soil environment, reduce soil nitrous oxide (N2O) emissions, and increase crop yields. ADI increased soil temperature, oxygen concentration, and ammonium and nitrate concentrations in the upper soil layers, but decreased them at deeper levels. ADI also increased soil microbial activity. Combining ADI with appropriate nitrogen application rates can enhance soil productivity and mitigate N2O emissions.
AGRICULTURAL WATER MANAGEMENT
(2023)
Article
Agronomy
Mueen Alam Khan, Muhammad Umar Ayyub, Ayesha Bashir, Beena Alam
Summary: Due to environmental fluctuations, wheat scientists need to develop new heat tolerant cultivars to increase and stabilize wheat production. A study was conducted to evaluate the genetic variability of twenty wheat genotypes under climate change scenarios. Significant differences were found among all genotypes for various morpho-phenological traits, and stronger correlations were observed under terminal heat stress, indicating the adversities of heat stress. Principal component analysis showed that the first three components explained a significant amount of cumulative variability in genotypes, and yield per plant had the highest variability. SUBHANI-21 exhibited the highest yield under heat stress, followed by LASANI-08 and UJALLA-16 for thousand grain weight. Selection of positively associated traits and utilization of tolerant genotypes with heat stress tolerance potential in future wheat breeding programs is important for developing high yielding and heat resilient genotypes.
Article
Environmental Sciences
E. Qoreishi, E. Rezaei-Chiyaneh, H. Mahdavikia, A. Rahimi, M. Gheshlaghi, D. Plaza-Bonilla, K. H. M. Siddique
Summary: This study explored the influence of different cropping patterns and arbuscular mycorrhizal fungi (AMF) inoculation on the productivity of kidney bean and the essence quantity and quality of lemon balm. The results showed that monoculture produced the highest dry matter yield of lemon balm and seed yield of kidney bean, while AMF inoculation increased the dry matter yields of lemon balm and seed yield of kidney bean. The LB/45 KB intercropping ratio with AMF treatment produced the highest essence content of lemon balm, and AMF inoculation enhanced essence productivity.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2023)
Editorial Material
Agronomy
M. Iqbal R. Khan, Nafees A. A. Khan, Adriano Sofo, Kadambot H. M. Siddique
Article
Plant Sciences
Yadollah Taghizadeh, Reza Amirnia, Esmaeil Rezaei-Chiyaneh, Mahdi Ghiyasi, Bahar S. S. Razavi, Kadambot H. M. Siddique
Summary: Implementing intercropping systems with biofertilizer applications is a sustainable strategy for improving the quantity and quality of plant products. This study found that using a biofertilizer application in the 2S:4 KB intercropping ratio resulted in the highest soil microbial and enzyme activities. Additionally, applying biofertilizer to the monocrops produced higher seed yields for sesame and kidney bean. The 2S:2 KB and 4S:2 KB intercropping ratios with biofertilizer application were identified as environmentally-friendly solutions for improving sesame oil quantity and quality.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2023)
Article
Genetics & Heredity
Muhammad Khuram Razzaq, Reena Rani, Guangnan Xing, Yufei Xu, Ghulam Raza, Muqadas Aleem, Shahid Iqbal, Muhammad Arif, Zahid Mukhtar, Henry T. Nguyen, Rajeev K. Varshney, Kadambot H. M. Siddique, Junyi Gai
Summary: This study characterized J-protein genes in soybean and identified those highly expressed and responsive during flower and seed development. The phylogeny, structure, motif analysis, chromosome location, and expression of these genes were also investigated. The findings suggest that J-protein genes could be involved in the growth period of soybean and provide a basis for further functional research.
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Parasitology
Muhammad Sajid Ramzan, Muhammad Suleman, Muhammad Imran Rashid, Haroon Akbar, Muhammad Avais
Summary: This study evaluated the cell-mediated immune responses in calves administered with two types of T. annulata vaccine (live attenuated and killed). The results showed a significant increase in CD4+ and CD8+ T cells in immunized calves. There was a significant difference in the CD8+ T cell response between the post-challenge stage of killed and live vaccine, while no other difference was found at both pre- and post-immunization stages.
PARASITOLOGY RESEARCH
(2023)
Review
Plant Sciences
Parul Sharma, Nita Lakra, Alisha Goyal, Yogesh K. Ahlawat, Abbu Zaid, Kadambot H. M. Siddique
Summary: Lipids play a crucial role in protecting plant cells and mediating responses to abiotic stress. Recent studies have shown that lipids can act as signal mediators in reducing stress responses and activating defense systems in plants. This review focuses on the synthesis of various signaling lipids and their roles in abiotic stress tolerance, providing valuable insights for further investigation into the interactions between plant lipids and abiotic stress.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Li Wang, Peina Lu, Shoujiang Feng, Chantal Hamel, Dandi Sun, Kadambot H. M. Siddique, Gary Y. Gan
Summary: This comprehensive review examines the effects of agricultural land use and crop/soil management on soil health, and provides feasible strategies for improving soil health.
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2024)
Article
Soil Science
Xingyun Qi, Guang Yang, Yi Li, Zhenan Hou, Penghui Shi, Shibin Wang, Xiaofang Wang, Jiaping Liang, Benhua Sun, Kadambot H. M. Siddique, Shufang Wu, Hao Feng, Xiaohong Tian, Qiang Yu, Xiangwen Xie
Summary: Biochar application can significantly increase the Na+, K+, and nutrient contents in cotton and sugarbeet fields, improving the chemical environment of saline-alkali soil. Soil salinity is highest in inter-rows and accumulates at a depth of 0-60 cm. The recommended optimal biochar application rate is 10 t ha 1, and sugarbeet is an effective crop for reducing soil salinity.
SOIL & TILLAGE RESEARCH
(2024)
Article
Soil Science
Kadambot H. M. Siddique, Nanthi Bolan, Abdul Rehman, Muhammad Farooq
Summary: Plants capture carbon dioxide through photosynthesis and store it as biomass. The roots of plants play a crucial role in transferring carbon into the soil's organic carbon pool. Implementing effective agricultural practices can sequester carbon in the long term and have multiple co-benefits.
SOIL & TILLAGE RESEARCH
(2024)