Time-resolved cryo-EM using a combination of droplet microfluidics with on-demand jetting

Gating choreography and mechanism of the human proton-activated chloride channel ASOR

(2022) Chongyuan Wang et al.   Science Advances

Structure of ATP synthase under strain during catalysis

(2022) Hui Guo et al.   Nature Communications

Effects of cryo-EM cooling on structural ensembles

(2022) Lars V. Bock et al.   Nature Communications

Coma-corrected rapid single-particle cryo-EM data collection on the CRYO ARM 300

(2021) Rouslan G. Efremov et al.   Acta Crystallographica Section D-Structural Biology

Activation pathway of a G protein-coupled receptor uncovers conformational intermediates as targets for allosteric drug design

(2021) Shaoyong Lu et al.   Nature Communications

Using photocaging for fast time-resolved structural biology studies

(2021) Diana C. F. Monteiro et al.   Acta Crystallographica Section D-Structural Biology

New tools for automated cryo-EM single-particle analysis in RELION-4.0

(2021) Dari Kimanius et al.   BIOCHEMICAL JOURNAL

Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1

(2020) Jasenko Zivanov et al.   IUCrJ

Sample deposition onto cryo-EM grids: from sprays to jets and back

(2020) David P. Klebl et al.   Acta Crystallographica Section D-Structural Biology

Modular microfluidics enables kinetic insight from time-resolved cryo-EM

(2020) Märt-Erik Mäeots et al.   Nature Communications

Assessing the JEOL CRYO ARM 300 for high-throughput automated single-particle cryo-EM in a multiuser environment

(2020) Marcus Fislage et al.   IUCrJ

UCSF ChimeraX : Structure visualization for researchers, educators, and developers

(2020) Eric F. Pettersen et al.   PROTEIN SCIENCE

Need for Speed: Examining Protein Behavior during CryoEM Grid Preparation at Different Timescales

(2020) David P. Klebl et al.   STRUCTURE

Late steps in bacterial translation initiation visualized using time-resolved cryo-EM

(2019) Sandip Kaledhonkar et al.   NATURE

Conformation space of a heterodimeric ABC exporter under turnover conditions

(2019) Susanne Hofmann et al.   NATURE

Rotary substates of mitochondrial ATP synthase reveal the basis of flexible F1-Fo coupling

(2019) Bonnie J. Murphy et al.   SCIENCE

Fast time-resolved NMR with non-uniform sampling

(2019) Dariusz Gołowicz et al.   PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

A cryo-EM grid preparation device for time-resolved structural studies

(2019) Dimitrios Kontziampasis et al.   IUCrJ

Current Developments in Coot for Macromolecular Model Building of Electron Cryo‐microscopy and Crystallographic Data

(2019) Ana Casañal et al.   PROTEIN SCIENCE

Shake-it-off: a simple ultrasonic cryo-EM specimen-preparation device

(2019) John L. Rubinstein et al.   Acta Crystallographica Section D-Structural Biology

Proteins, interfaces, and cryo-EM grids

(2018) Robert M. Glaeser   CURRENT OPINION IN COLLOID & INTERFACE SCIENCE

Reducing effects of particle adsorption to the air–water interface in cryo-EM

(2018) Alex J. Noble et al.   NATURE METHODS

Cryo-EM structure of respiratory complex I at work

(2018) Kristian Parey et al.   eLife

Time-resolved cryo-electron microscopy: Recent progress

(2017) Joachim Frank   JOURNAL OF STRUCTURAL BIOLOGY

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

MolProbity: More and better reference data for improved all-atom structure validation

(2017) Christopher J. Williams et al.   PROTEIN SCIENCE

A Fast and Effective Microfluidic Spraying-Plunging Method for High-Resolution Single-Particle Cryo-EM

(2017) Xiangsong Feng et al.   STRUCTURE

Measuring the effects of particle orientation to improve the efficiency of electron cryomicroscopy

(2017) Katerina Naydenova et al.   Nature Communications

The Physics and Physical Chemistry of Molecular Machines

(2016) R. Dean Astumian et al.   CHEMPHYSCHEM

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

Gas-assisted annular microsprayer for sample preparation for time-resolved cryo-electron microscopy

(2014) Zonghuan Lu et al.   JOURNAL OF MICROMECHANICS AND MICROENGINEERING

The ensemble nature of allostery

(2014) Hesam N. Motlagh et al.   NATURE

Fast temperature measurement following single laser-induced cavitation inside a microfluidic gap

(2014) Pedro A. Quinto-Su et al.   Scientific Reports

ATP-Triggered Conformational Changes Delineate Substrate-Binding and -Folding Mechanics of the GroEL Chaperonin

(2012) Daniel K. Clare et al.   CELL

Spotiton: A prototype for an integrated inkjet dispense and vitrification system for cryo-TEM

(2012) Tilak Jain et al.   JOURNAL OF STRUCTURAL BIOLOGY

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

High-speed droplet generation on demand driven by pulse laser-induced cavitation

(2011) Sung-Yong Park et al.   LAB ON A CHIP

Dynamics of microfluidic droplets

(2010) Charles N. Baroud et al.   LAB ON A CHIP

Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy

(2010) Niels Fischer et al.   NATURE

Binding, activation and modulation of Cys-loop receptors

(2010) Paul S. Miller et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Monolithic microfluidic mixing–spraying devices for time-resolved cryo-electron microscopy

(2009) Zonghuan Lu et al.   JOURNAL OF STRUCTURAL BIOLOGY

Passive microfluidic device for submillisecond mixing

(2009) Zonghuan Lu et al.   SENSORS AND ACTUATORS B-CHEMICAL

Monitoring Protein Conformation along the Pathway of Chaperonin-Assisted Folding

(2008) Shruti Sharma et al.   CELL

Implementation of a flash-photolysis system for time-resolved cryo-electron microscopy

(2008) Tanvir R. Shaikh et al.   JOURNAL OF STRUCTURAL BIOLOGY