Establishing a versatile toolkit of flux enhanced strains and cell extracts for pathway prototyping

Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale

(2022) Fungmin Eric Liew et al.   NATURE BIOTECHNOLOGY

Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria

(2022) Bastian Vögeli et al.   Nature Communications

Considering Strain Variation and Non-Type Strains for Yeast Metabolic Engineering Applications

(2022) Xiunan Yi et al.   Life-Basel

Using fungible biosensors to evolve improved alkaloid biosyntheses

(2022) Simon d’Oelsnitz et al.   Nature Chemical Biology

Streptomyces cell-free systems for natural product discovery and engineering

(2022) Simon J. Moore et al.   NATURAL PRODUCT REPORTS

Automated Rational Strain Construction Based on High-Throughput Conjugation

(2021) Niklas Tenhaef et al.   ACS Synthetic Biology

Synthetic Biochemistry: The Bio-inspired Cell-Free Approach to Commodity Chemical Production

(2020) James U. Bowie et al.   TRENDS IN BIOTECHNOLOGY

Cell-free prototyping of limonene biosynthesis using cell-free protein synthesis

(2020) Quentin M. Dudley et al.   METABOLIC ENGINEERING

Multicomponent Microscale Biosynthesis of Unnatural Cyanobacterial Indole Alkaloids

(2020) Yogan Khatri et al.   ACS Synthetic Biology

Cell‐free exploration of the natural product chemical space

(2020) Jonathan W. Bogart et al.   CHEMBIOCHEM

You get what you screen for: on the value of fermentation characterization in high-throughput strain improvements in industrial settings

(2020) Maren Wehrs et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Metabolic engineering of Saccharomyces cerevisiae for the de novo production of psilocybin and related tryptamine derivatives

(2020) N. Milne et al.   METABOLIC ENGINEERING

In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design

(2020) Ashty S. Karim et al.   Nature Chemical Biology

Cell-free styrene biosynthesis at high titers

(2020) William S. Grubbe et al.   METABOLIC ENGINEERING

Biosynthesis of medicinal tropane alkaloids in yeast

(2020) Prashanth Srinivasan et al.   NATURE

Cell-Free Biocatalysis for the Production of Platform Chemicals

(2020) Peter L. Bergquist et al.   Frontiers in Energy Research

Rewiring yeast metabolism to synthesize products beyond ethanol

(2020) Francesca V. Gambacorta et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of l-DOPA from d-glucose

(2019) Eric Fordjour et al.   Microbial Cell Factories

High-throughput mapping of CoA metabolites by SAMDI-MS to optimize the cell-free biosynthesis of HMG-CoA

(2019) Patrick T. O’Kane et al.   Science Advances

A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

(2019) Weston Kightlinger et al.   Nature Communications

Recent trends in metabolic engineering of microbial chemical factories

(2019) Yi Liu et al.   CURRENT OPINION IN BIOTECHNOLOGY

Modular Ligation Extension of Guide RNA Operons (LEGO) for Multiplexed dCas9 Regulation of Metabolic Pathways in Saccharomyces cerevisiae

(2018) Matthew Deaner et al.   Biotechnology Journal

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases

(2018) Weston Kightlinger et al.   Nature Chemical Biology

Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods

(2018) Wei Liu et al.   Nature Communications

Modular Ligation Extension of Guide RNA Operons (LEGO) for Multiplexed dCas9 Regulation of Metabolic Pathways in Saccharomyces cerevisiae

(2018) Matthew Deaner et al.   Biotechnology Journal

Recent advancements in fungal-derived fuel and chemical production and commercialization

(2018) Holly Ekas et al.   CURRENT OPINION IN BIOTECHNOLOGY

Systematic testing of enzyme perturbation sensitivities via graded dCas9 modulation in Saccharomyces cerevisiae

(2017) Matthew Deaner et al.   METABOLIC ENGINEERING

Biosensor-based engineering of biosynthetic pathways

(2016) Jameson K Rogers et al.   CURRENT OPINION IN BIOTECHNOLOGY

A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery

(2016) Ashty S. Karim et al.   METABOLIC ENGINEERING

Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking

(2016) Mingxun Wang et al.   NATURE BIOTECHNOLOGY

Metabolic Burden: Cornerstones in Synthetic Biology and Metabolic Engineering Applications

(2016) Gang Wu et al.   TRENDS IN BIOTECHNOLOGY

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Complete biosynthesis of opioids in yeast

(2015) S. Galanie et al.   SCIENCE

High-throughput preparation methods of crude extract for robust cell-free protein synthesis

(2015) Yong-Chan Kwon et al.   Scientific Reports

A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly

(2015) Michael E. Lee et al.   ACS Synthetic Biology

Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis

(2013) C. Eric Hodgman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

Metabolic engineering of muconic acid production in Saccharomyces cerevisiae

(2012) Kathleen A. Curran et al.   METABOLIC ENGINEERING

Recent advances in engineering the central carbon metabolism of industrially important bacteria

(2012) Maria Papagianni   Microbial Cell Factories

MZmine 2: Modular framework for processing, visualizing, and analyzing mass spectrometry-based molecular profile data

(2010) Tomáš Pluskal et al.   BMC BIOINFORMATICS

Production of Glucaric Acid from a Synthetic Pathway in Recombinant Escherichia coli

(2008) T. S. Moon et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY