Modular Ligation Extension of Guide RNA Operons (LEGO) for Multiplexed dCas9 Regulation of Metabolic Pathways in Saccharomyces cerevisiae

RewiringYarrowia lipolyticatoward triacetic acid lactone for materials generation

(2018) Kelly A. Markham et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR/Cas9-mediated gene knockout screens and target identification via whole-genome sequencing uncover host genes required for picornavirus infection

(2017) Heon Seok Kim et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Systematic testing of enzyme perturbation sensitivities via graded dCas9 modulation in Saccharomyces cerevisiae

(2017) Matthew Deaner et al.   METABOLIC ENGINEERING

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

(2017) Jiazhang Lian et al.   Nature Communications

Enabling Graded and Large-Scale Multiplex of Desired Genes Using a Dual-Mode dCas9 Activator in Saccharomyces cerevisiae

(2017) Matthew Deaner et al.   ACS Synthetic Biology

Multiplex gene regulation by CRISPR-ddCpf1

(2017) Xiaochun Zhang et al.   Cell Discovery

Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering

(2016) Addison V. Wright et al.   CELL

Engineering cytosolic acetyl-coenzyme A supply in Saccharomyces cerevisiae : Pathway stoichiometry, free-energy conservation and redox-cofactor balancing

(2016) Harmen M. van Rossum et al.   METABOLIC ENGINEERING

CRISPR interference as a titratable, trans -acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp . strain PCC 7002

(2016) Gina C. Gordon et al.   METABOLIC ENGINEERING

CRISPRi engineering E. coli for morphology diversification

(2016) Dina Elhadi et al.   METABOLIC ENGINEERING

Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR–Cas9-Mediated Genome Editing in Yarrowia lipolytica

(2016) Cory M. Schwartz et al.   ACS Synthetic Biology

Multiplex CRISPR/Cas9-based genome engineering enhanced by Drosha-mediated sgRNA-shRNA structure

(2016) Qiang Yan et al.   Scientific Reports

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

Efficient production of 2,3-butanediol in Saccharomyces cerevisiae by eliminating ethanol and glycerol production and redox rebalancing

(2015) Sujin Kim et al.   METABOLIC ENGINEERING

Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae

(2015) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

(2015) Silvana Konermann et al.   NATURE

Highly efficient Cas9-mediated transcriptional programming

(2015) Alejandro Chavez et al.   NATURE METHODS

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Expression of CRISPR/Cas single guide RNAs using small tRNA promoters

(2015) Adam L. Mefferd et al.   RNA

CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli

(2015) Brady F. Cress et al.   ACS Synthetic Biology

Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells

(2014) Lior Nissim et al.   MOLECULAR CELL

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Transfer RNA Post-Transcriptional Processing, Turnover, and Subcellular Dynamics in the YeastSaccharomyces cerevisiae

(2013) Anita K. Hopper   GENETICS

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

High-level semi-synthetic production of the potent antimalarial artemisinin

(2013) C. J. Paddon et al.   NATURE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Optimization of a Yeast RNA Interference System for Controlling Gene Expression and Enabling Rapid Metabolic Engineering

(2013) Nathan C. Crook et al.   ACS Synthetic Biology

Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas

(2013) Fahim Farzadfard et al.   ACS Synthetic Biology

Transcription by RNA polymerase III: more complex than we thought

(2011) Robert J. White   NATURE REVIEWS GENETICS