Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods

Design of a synthetic yeast genome

(2017) Sarah M. Richardson et al.   SCIENCE

Effect of Genomic Integration Location on Heterologous Protein Expression and Metabolic Engineering in E. coli

(2017) Jacob A. Englaender et al.   ACS Synthetic Biology

EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors

(2017) Andrea Martella et al.   ACS Synthetic Biology

CRISPR/Cas9 advances engineering of microbial cell factories

(2016) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

Yeast metabolic chassis designs for diverse biotechnological products

(2016) Paula Jouhten et al.   Scientific Reports

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

SCRaMbLE generates designed combinatorial stochastic diversity in synthetic chromosomes

(2015) Yue Shen et al.   GENOME RESEARCH

An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose

(2015) William C DeLoache et al.   Nature Chemical Biology

Versatile genetic assembly system (VEGAS) to assemble pathways for expression inS. cerevisiae

(2015) Leslie A. Mitchell et al.   NUCLEIC ACIDS RESEARCH

YeastFab: the design and construction of standard biological parts for metabolic engineering inSaccharomyces cerevisiae

(2015) Yakun Guo et al.   NUCLEIC ACIDS RESEARCH

A tetO Toolkit To Alter Expression of Genes in Saccharomyces cerevisiae

(2015) Josh T. Cuperus et al.   ACS Synthetic Biology

Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units

(2015) Neta Agmon et al.   ACS Synthetic Biology

Novel Heterotypic Rox Sites for Combinatorial Dre Recombination Strategies

(2015) Katherine Chuang et al.   G3-Genes Genomes Genetics

Three-dimensional modeling of the P. falciparum genome during the erythrocytic cycle reveals a strong connection between genome architecture and gene expression

(2014) F. Ay et al.   GENOME RESEARCH

A microbial biomanufacturing platform for natural and semisynthetic opioids

(2014) Kate Thodey et al.   Nature Chemical Biology

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development

(2014) Chris J. Paddon et al.   NATURE REVIEWS MICROBIOLOGY

PaperClip: rapid multi-part DNA assembly from existing libraries

(2014) Maryia Trubitsyna et al.   NUCLEIC ACIDS RESEARCH

Total Synthesis of a Functional Designer Eukaryotic Chromosome

(2014) N. Annaluru et al.   SCIENCE

Compartmentalization of metabolic pathways in yeast mitochondria improves the production of branched-chain alcohols

(2013) José L Avalos et al.   NATURE BIOTECHNOLOGY

Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination

(2013) Sean D. Colloms et al.   NUCLEIC ACIDS RESEARCH

Implementation of stable and complex biological systems through recombinase-assisted genome engineering

(2013) Christine Nicole S. Santos et al.   Nature Communications

Single Cell Visualization of Yeast Gene Expression Shows Correlation of Epigenetic Switching between Multiple Heterochromatic Regions through Multiple Generations

(2013) Yasunobu Mano et al.   PLOS BIOLOGY

Mechanism for epigenetic variegation of gene expression at yeast telomeric heterochromatin

(2012) T. Kitada et al.   GENES & DEVELOPMENT

The future of metabolic engineering and synthetic biology: Towards a systematic practice

(2012) Vikramaditya G. Yadav et al.   METABOLIC ENGINEERING

Synthetic biology and the development of tools for metabolic engineering

(2012) Jay D. Keasling   METABOLIC ENGINEERING

Synthetic Biology and Metabolic Engineering

(2012) Gregory Stephanopoulos   ACS Synthetic Biology

A combined approach of classical mutagenesis and rational metabolic engineering improves rapamycin biosynthesis and provides insights into methylmalonyl-CoA precursor supply pathway in Streptomyces hygroscopicus ATCC 29253

(2011) Won Seok Jung et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Synthetic chromosome arms function in yeast and generate phenotypic diversity by design

(2011) Jessica S. Dymond et al.   NATURE

Synergies between synthetic biology and metabolic engineering

(2011) Jens Nielsen et al.   NATURE BIOTECHNOLOGY

Targeted Genome Editing Across Species Using ZFNs and TALENs

(2011) Andrew J. Wood et al.   SCIENCE

Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice

(2009) K. Anastassiadis et al.   Disease Models & Mechanisms

The Mother Enrichment Program: A Genetic System for Facile Replicative Life Span Analysis in Saccharomyces cerevisiae

(2009) D. L. Lindstrom et al.   GENETICS

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Synthetic protein scaffolds provide modular control over metabolic flux

(2009) John E Dueber et al.   NATURE BIOTECHNOLOGY

Coding-Sequence Determinants of Gene Expression in Escherichia coli

(2009) G. Kudla et al.   SCIENCE

Characterization of chromosomal integration sites for heterologous gene expression inSaccharomyces cerevisiae

(2009) Dongmei Bai Flagfeldt et al.   YEAST

Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

(2008) Sung Kuk Lee et al.   CURRENT OPINION IN BIOTECHNOLOGY

Genome-Wide Analysis of the Effects of Location and Number of Stress Response Elements on Gene Expression in Saccharomyces cerevisiae

(2008) Katsunori Yoshikawa et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING