Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases

Production of homogeneous glycoprotein with multisite modifications by an engineeredN-glycosyltransferase mutant

(2017) Qitao Song et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A biosynthetic route for polysialylating proteins in Escherichia coli

(2017) Timothy G. Keys et al.   METABOLIC ENGINEERING

TheN-linking glycosylation system fromActinobacillus pleuropneumoniaeis required for adhesion and has potential use in glycoengineering

(2017) Jon Cuccui et al.   Open Biology

Glyco-seek: Ultrasensitive Detection of Protein-Specific Glycosylation by Proximity Ligation Polymerase Chain Reaction

(2016) Peter V. Robinson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Loss ofO-GlcNAc glycosylation in forebrain excitatory neurons induces neurodegeneration

(2016) Andrew C. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

N-acetylgalactosaminyltransferases in cancer

(2016) Muhammad Ramzan Manwar Hussain et al.   Oncotarget

Engineered CHO cells for production of diverse, homogeneous glycoproteins

(2015) Zhang Yang et al.   NATURE BIOTECHNOLOGY

The active site of O-GlcNAc transferase imposes constraints on substrate sequence

(2015) Shalini Pathak et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A common glycan structure on immunoglobulin G for enhancement of effector functions

(2015) Chin-Wei Lin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The active site of O-GlcNAc transferase imposes constraints on substrate sequence

(2015) Shalini Pathak et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Acetyltransferase p300/CBP Associated Factor (PCAF) Regulates Crosstalk-Dependent Acetylation of Histone H3 by Distal Site Recognition

(2014) James R. Kornacki et al.   ACS Chemical Biology

A peptide panel investigation reveals the acceptor specificity of O-GlcNAc transferase

(2014) Xiaoyan Liu et al.   FASEB JOURNAL

Probing polypeptide GalNAc-transferase isoform substrate specificities by in vitro analysis

(2014) Yun Kong et al.   GLYCOBIOLOGY

Substrate Specificity of CytoplasmicN-Glycosyltransferase

(2014) Andreas Naegeli et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Microarray Discovery of New OGT Substrates: The Medulloblastoma Oncogene OTX2 Is O-GlcNAcylated

(2014) Rodrigo F. Ortiz-Meoz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Low-Flow Sheathless Capillary Electrophoresis–Mass Spectrometry for Sensitive Glycoform Profiling of Intact Pharmaceutical Proteins

(2013) Rob Haselberg et al.   ANALYTICAL CHEMISTRY

Profiling Deacetylase Activities in Cell Lysates with Peptide Arrays and SAMDI Mass Spectrometry

(2013) Hsin-Yu Kuo et al.   ANALYTICAL CHEMISTRY

Relative Quantitation of Glycoisoforms of Intact Apolipoprotein C3 in Human Plasma by Liquid Chromatography–High-Resolution Mass Spectrometry

(2013) Wenying Jian et al.   ANALYTICAL CHEMISTRY

A two-step enzymatic glycosylation of polypeptides with complex N -glycans

(2013) Joseph V. Lomino et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Precision mapping of the human O-GalNAc glycoproteome through SimpleCell technology

(2013) Catharina Steentoft et al.   EMBO JOURNAL

Molecular Analysis of an AlternativeN-Glycosylation Machinery by Functional Transfer fromActinobacillus pleuropneumoniaetoEscherichia coli

(2013) Andreas Naegeli et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The carbohydrate-active enzymes database (CAZy) in 2013

(2013) Vincent Lombard et al.   NUCLEIC ACIDS RESEARCH

Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites

(2013) Amin Espah Borujeni et al.   NUCLEIC ACIDS RESEARCH

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Realizing the promise of chemical glycobiology

(2013) Lai-Xi Wang et al.   Chemical Science

Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation

(2013) Seok Hoon Hong et al.   ACS Synthetic Biology

An engineered eukaryotic protein glycosylation pathway in Escherichia coli

(2012) Juan D Valderrama-Rincon et al.   Nature Chemical Biology

Discovery of glycosyltransferases using carbohydrate arrays and mass spectrometry

(2012) Lan Ban et al.   Nature Chemical Biology

Probing isoform-specific functions of polypeptide GalNAc-transferases using zinc finger nuclease glycoengineered SimpleCells

(2012) K. T.- B. G. Schjoldager et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Structural Insights into the Glycosyltransferase Activity of theActinobacillus pleuropneumoniaeHMW1C-like Protein

(2011) Fumihiro Kawai et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

CytoplasmicN-Glycosyltransferase ofActinobacillus pleuropneumoniaeIs an Inverting Enzyme and Recognizes the NX(S/T) Consensus Sequence

(2011) Flavio Schwarz et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega

(2011) F. Sievers et al.   Molecular Systems Biology

Structure of human O-GlcNAc transferase and its complex with a peptide substrate

(2011) Michael B. Lazarus et al.   NATURE

High-Throughput Screening of Small Molecule Libraries using SAMDI Mass Spectrometry

(2011) Zachary A. Gurard-Levin et al.   ACS Combinatorial Science

Proteome-wide post-translational modification statistics: frequency analysis and curation of the swiss-prot database

(2011) George A. Khoury et al.   Scientific Reports

Production of Secretory and Extracellular N-Linked Glycoproteins inEscherichia coli

(2010) Adam C. Fisher et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Identification of protein O-GlcNAcylation sites using electron transfer dissociation mass spectrometry on native peptides

(2009) R. J. Chalkley et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enzymatic Glycosylation of Peptide Arrays on Gold Surfaces

(2008) Nicolas Laurent et al.   CHEMBIOCHEM

SPOT Synthesis of Peptide Arrays on Self-Assembled Monolayers and their Evaluation as Enzyme Substrates

(2008) Nicolas Laurent et al.   CHEMBIOCHEM

TheHaemophilus influenzaeHMW1 Adhesin Is a Glycoprotein with an UnusualN-Linked Carbohydrate Modification

(2008) Julia Gross et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Phosphoinositide signalling links O-GlcNAc transferase to insulin resistance

(2008) Xiaoyong Yang et al.   NATURE

Cetuximab-Induced Anaphylaxis and IgE Specific for Galactose-α-1,3-Galactose

(2008) Christine H. Chung et al.   NEW ENGLAND JOURNAL OF MEDICINE

Rapid Expression and Purification of 100 nmol Quantities of Active Protein Using Cell-Free Protein Synthesis

(2004) Michael C. Jewett et al.   BIOTECHNOLOGY PROGRESS