Gene editing of putative cAMP and Ca2+‐regulated proteins using an efficient cloning‐free CRISPR/Cas9 system in Trypanosoma cruzi

Identification of inhibitors for the transmembrane Trypanosoma cruzi eIF2α kinase relevant for parasite proliferation

(2023) Tiago de Paula Marcelino et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

H2B.V demarcates divergent strand-switch regions, some tDNA loci, and genome compartments in Trypanosoma cruzi and affects parasite differentiation and host cell invasion

(2022) Juliana Nunes Rosón et al.   PLoS Pathogens

Trypanin Disruption Affects the Motility and Infectivity of the Protozoan Trypanosoma cruzi

(2022) Jose L. Saenz-Garcia et al.   Frontiers in Cellular and Infection Microbiology

A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cells

(2022) Seung Bum Park et al.   Molecular Therapy-Nucleic Acids

Trypanosoma cruzi Letm1 is involved in mitochondrial Ca 2+ transport, and is essential for replication, differentiation, and host cell invasion

(2021) Guilherme Rodrigo RM dos Santos et al.   FASEB JOURNAL

A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in Trypanosoma cruzi

(2021) Noopur Dave et al.   eLife

The long and winding road of reverse genetics in Trypanosoma cruzi

(2021) Miguel A. Chiurillo et al.   Microbial Cell

Species-selective targeting of pathogens revealed by the atypical structure and active site of Trypanosoma cruzi histone deacetylase DAC2

(2021) Martin Marek et al.   Cell Reports

Effective Genome Editing in Leishmania (Viannia) braziliensis Stably Expressing Cas9 and T7 RNA Polymerase

(2021) Caroline R. Espada et al.   Frontiers in Cellular and Infection Microbiology

The Influence of Environmental Cues on the Development of Trypanosoma cruzi in Triatominae Vector

(2020) Raíssa de Fátima Pimentel Melo et al.   Frontiers in Cellular and Infection Microbiology

Essential roles for deubiquitination in Leishmania life cycle progression

(2020) Andreas Damianou et al.   PLoS Pathogens

Signaling pathways involved in environmental sensing in Trypanosoma cruzi

(2020) Noelia Lander et al.   MOLECULAR MICROBIOLOGY

IP3 receptor-mediated Ca2+ release from acidocalcisomes regulates mitochondrial bioenergetics and prevents autophagy in Trypanosoma cruzi

(2020) Miguel A. Chiurillo et al.   CELL CALCIUM

Testing the CRISPR-Cas9 and glmS ribozyme systems in Leishmania tarentolae

(2020) Gino L. Turra et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Signal Transduction Pathways as Therapeutic Target for Chagas Disease

(2019) Alejandra C. Schoijet et al.   CURRENT MEDICINAL CHEMISTRY

Strategies to Increase On-Target and Reduce Off-Target Effects of the CRISPR/Cas9 System in Plants

(2019) Zahra Hajiahmadi et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

State‐of‐the‐art CRISPR /Cas9 Technology for Genome Editing in Trypanosomatids

(2019) Noelia Lander et al.   JOURNAL OF EUKARYOTIC MICROBIOLOGY

Genetic dissection of a Leishmania flagellar proteome demonstrates requirement for directional motility in sand fly infections

(2019) Tom Beneke et al.   PLoS Pathogens

Evaluation of clan CD C11 peptidase PNT1 and other Leishmania mexicana cysteine peptidases as potential drug targets

(2019) Jaspreet Singh Grewal et al.   BIOCHIMIE

cAMP: From Long-Range Second Messenger to Nanodomain Signalling

(2018) Nshunge Musheshe et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Expanding the toolbox for Trypanosoma cruzi: A parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid in vivo localisation and phenotyping

(2018) Fernanda Cristina Costa et al.   PLoS Neglected Tropical Diseases

Genetically Validated Drug Targets in Leishmania: Current Knowledge and Future Prospects

(2018) Nathaniel G. Jones et al.   ACS Infectious Diseases

Calcium-sensitive pyruvate dehydrogenase phosphatase is required for energy metabolism, growth, differentiation, and infectivity of Trypanosoma cruzi

(2018) Noelia Lander et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit

(2017) Daniel Martel et al.   Biomed Research International

A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids

(2017) Tom Beneke et al.   Royal Society Open Science

CRISPR/Cas9-mediated endogenous C-terminal Tagging ofTrypanosoma cruziGenes Reveals the Acidocalcisome Localization of the Inositol 1,4,5-Trisphosphate Receptor

(2016) Noelia Lander et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Genome Editing by CRISPR/Cas9: A Game Change in the Genetic Manipulation of Protists

(2016) Noelia Lander et al.   JOURNAL OF EUKARYOTIC MICROBIOLOGY

Calcium signaling in trypanosomatid parasites

(2015) Roberto Docampo et al.   CELL CALCIUM

In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of adenylyl cyclase expression

(2015) Afsaneh Hamedi et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Identification of novel cyclic nucleotide binding proteins in Trypanosoma cruzi

(2015) Adriana V. Jäger et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

CRISPR/Cas9 mediated genome engineering in Drosophila

(2014) Andrew Bassett et al.   METHODS

Proteomic Analysis of Intact Flagella of ProcyclicTrypanosoma bruceiCells Identifies Novel Flagellar Proteins with Unique Sub-localization and Dynamics

(2014) Ines Subota et al.   MOLECULAR & CELLULAR PROTEOMICS

Regulators of Trypanosoma brucei Cell Cycle Progression and Differentiation Identified Using a Kinome-Wide RNAi Screen

(2014) Nathaniel G. Jones et al.   PLoS Pathogens

Proteomic Analysis of the Acidocalcisome, an Organelle Conserved from Bacteria to Human Cells

(2014) Guozhong Huang et al.   PLoS Pathogens

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Essential regulation of cell bioenergetics in Trypanosoma brucei by the mitochondrial calcium uniporter

(2013) Guozhong Huang et al.   Nature Communications

Identification of novel families and classification of the C2 domain superfamily elucidate the origin and evolution of membrane targeting activities in eukaryotes

(2010) Dapeng Zhang et al.   GENE