A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cells

Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus

(2021) Do Yon Kim et al.   NATURE BIOTECHNOLOGY

In vivo delivery of CRISPR-Cas9 therapeutics: Progress and challenges

(2021) Matthew Behr et al.   Acta Pharmaceutica Sinica B

Drug Inducible CRISPR/Cas Systems

(2019) Jingfang Zhang et al.   Computational and Structural Biotechnology Journal

A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells

(2018) Christopher A. Vakulskas et al.   NATURE MEDICINE

Efficient homology-directed gene editing by CRISPR/Cas9 in human stem and primary cells using tube electroporation

(2018) Xiaoyun Xu et al.   Scientific Reports

Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization

(2017) Serif Senturk et al.   Nature Communications

Evolution and Ecology of CRISPR

(2016) Edze R. Westra et al.   Annual Review of Ecology Evolution and Systematics

Tet-On Systems For Doxycycline-inducible Gene Expression

(2016) Atze T. Das et al.   CURRENT GENE THERAPY

Dual control system – A novel scaffolding architecture of an inducible regulatory device for the precise regulation of gene expression

(2016) L. Horbal et al.   METABOLIC ENGINEERING

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

(2016) Benjamin L Oakes et al.   NATURE BIOTECHNOLOGY

Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

(2016) Benjamin P Kleinstiver et al.   NATURE BIOTECHNOLOGY

Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells

(2016) Daesik Kim et al.   NATURE BIOTECHNOLOGY

Hepatic differentiation of human pluripotent stem cells in miniaturized format suitable for high-throughput screen

(2016) Arnaud Carpentier et al.   Stem Cell Research

CRISPR-Cas immunity in prokaryotes

(2015) Luciano A. Marraffini   NATURE

Inducible in vivo genome editing with CRISPR-Cas9

(2015) Lukas E Dow et al.   NATURE BIOTECHNOLOGY

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

(2015) Yuta Nihongaki et al.   NATURE BIOTECHNOLOGY

Highly efficient Cas9-mediated transcriptional programming

(2015) Alejandro Chavez et al.   NATURE METHODS

Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

(2015) Daesik Kim et al.   NATURE METHODS

Development of an intein-mediated split–Cas9 system for gene therapy

(2015) Dong-Jiunn Jeffery Truong et al.   NUCLEIC ACIDS RESEARCH

Generation of knock-in primary human T cells using Cas9 ribonucleoproteins

(2015) Kathrin Schumann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Transcriptional profiling of macrophages derived from monocytes and iPS cells identifies a conserved response to LPS and novel alternative transcription

(2015) Kaur Alasoo et al.   Scientific Reports

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

(2015) Masakazu Hashimoto et al.   Scientific Reports

An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells

(2014) Federico González et al.   Cell Stem Cell

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Engrafted human stem cell–derived hepatocytes establish an infectious HCV murine model

(2014) Arnaud Carpentier et al.   JOURNAL OF CLINICAL INVESTIGATION

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Improved vectors and genome-wide libraries for CRISPR screening

(2014) Neville E Sanjana et al.   NATURE METHODS

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

(2013) S. W. Cho et al.   GENOME RESEARCH

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

(2013) Seung Woo Cho et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Extensive Methylation of Promoter Sequences Silences Lentiviral Transgene Expression During Stem Cell Differentiation In Vivo

(2012) Friederike Herbst et al.   MOLECULAR THERAPY

Chromatin Insulator Elements Block Transgene Silencing in Engineered Human Embryonic Stem Cell Lines at a Defined Chromosome 13 Locus

(2011) Chad C. MacArthur et al.   STEM CELLS AND DEVELOPMENT

Improved Tet-responsive promoters with minimized background expression

(2010) Rainer Loew et al.   BMC BIOTECHNOLOGY