The use of CrossMAb technology for the generation of bi- and multispecific antibodies

Bispecific Anti-HIV-1 Antibodies with Enhanced Breadth and Potency

(2016) Stylianos Bournazos et al.   CELL

Engineered Bispecific Antibodies with Exquisite HIV-1-Neutralizing Activity

(2016) Yaoxing Huang et al.   CELL

Development of purification processes for fully human bispecific antibodies based upon modification of protein A binding avidity

(2016) Andrew D. Tustian et al.   mAbs

CODV-Ig, a universal bispecific tetravalent and multifunctional immunoglobulin format for medical applications

(2016) Anke Steinmetz et al.   mAbs

RG7386, a Novel Tetravalent FAP-DR5 Antibody, Effectively Triggers FAP-Dependent, Avidity-Driven DR5 Hyperclustering and Tumor Cell Apoptosis

(2016) P. Bru nker et al.   MOLECULAR CANCER THERAPEUTICS

Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival

(2016) Jonas Kloepper et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Preclinical pharmacokinetics, pharmacodynamics, and efficacy of RG7116: a novel humanized, glycoengineered anti-HER3 antibody

(2015) Georgina Meneses-Lorente et al.   CANCER CHEMOTHERAPY AND PHARMACOLOGY

Effect of Ang-2-VEGF-A Bispecific Antibody in Renal Cell Carcinoma

(2015) Hideharu Bessho et al.   CANCER INVESTIGATION

Bispecific antibodies

(2015) Roland E. Kontermann et al.   DRUG DISCOVERY TODAY

A Novel Antibody Engineering Strategy for Making Monovalent Bispecific Heterodimeric IgG Antibodies by Electrostatic Steering Mechanism

(2015) Zhi Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Development and validation of a novel SPR-based assay principle for bispecific molecules

(2015) C. Gassner et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Bispecific Antibodies Targeting Different Epitopes on the HIV-1 Envelope Exhibit Broad and Potent Neutralization

(2015) M. Asokan et al.   JOURNAL OF VIROLOGY

Fab-based bispecific antibody formats with robust biophysical properties and biological activity

(2015) Xiufeng Wu et al.   mAbs

Improving target cell specificity using a novel monovalent bispecific IgG design

(2015) Yariv Mazor et al.   mAbs

Heavy and light chain pairing of bivalent quadroma and knobs-into-holes antibodies analyzed by UHR-ESI-QTOF mass spectrometry

(2015) Wolfgang Schaefer et al.   mAbs

Selective Delivery of PEGylated Compounds to Tumor Cells by Anti-PEG Hybrid Antibodies

(2015) H.-Y. Tung et al.   MOLECULAR CANCER THERAPEUTICS

Alternative molecular formats and therapeutic applications for bispecific antibodies

(2015) Christoph Spiess et al.   MOLECULAR IMMUNOLOGY

Identification of Anti-EGFR and Anti-ErbB3 Dual Variable Domains Immunoglobulin (DVD-Ig) Proteins with Unique Activities

(2015) Jinming Gu et al.   PLoS One

Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG

(2015) Nicolas Fischer et al.   Nature Communications

A novel, native-format bispecific antibody triggering T-cell killing of B-cells is robustly active in mouse tumor models and cynomolgus monkeys

(2015) Eric J. Smith et al.   Scientific Reports

Combating HER2-overexpressing breast cancer through induction of calreticulin exposure by Tras-Permut CrossMab

(2015) Fan Zhang et al.   OncoImmunology

Postsurgical Adjuvant Tumor Therapy by Combining Anti-Angiopoietin-2 and Metronomic Chemotherapy Limits Metastatic Growth

(2014) Kshitij Srivastava et al.   CANCER CELL

Four-in-One Antibodies Have Superior Cancer Inhibitory Activity against EGFR, HER2, HER3, and VEGF through Disruption of HER/MET Crosstalk

(2014) S. Hu et al.   CANCER RESEARCH

A Novel Glycoengineered Bispecific Antibody Format for Targeted Inhibition of Epidermal Growth Factor Receptor (EGFR) and Insulin-like Growth Factor Receptor Type I (IGF-1R) Demonstrating Unique Molecular Properties

(2014) Juergen M. Schanzer et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Combating non-Hodgkin lymphoma by targeting both CD20 and HLA-DR through CD20–243 CrossMab

(2014) Lei Zhao et al.   mAbs

Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface

(2014) Steven M Lewis et al.   NATURE BIOTECHNOLOGY

Combined Inhibition of Tumor Necrosis Factor α and Interleukin-17 As a Therapeutic Opportunity in Rheumatoid Arthritis: Development and Characterization of a Novel Bispecific Antibody

(2014) Jens A. A. Fischer et al.   Arthritis & Rheumatology

Eradication of non-Hodgkin lymphoma through the induction of tumor-specific T-cell immunity by CD20-Flex BiFP

(2013) L. Zhao et al.   BLOOD

RG7116, a Therapeutic Antibody That Binds the Inactive HER3 Receptor and Is Optimized for Immune Effector Activation

(2013) C. Mirschberger et al.   CANCER RESEARCH

Ang-2-VEGF-A CrossMab, a Novel Bispecific Human IgG1 Antibody Blocking VEGF-A and Ang-2 Functions Simultaneously, Mediates Potent Antitumor, Antiangiogenic, and Antimetastatic Efficacy

(2013) Y. Kienast et al.   CLINICAL CANCER RESEARCH

Development of a Human IgG4 Bispecific Antibody for Dual Targeting of Interleukin-4 (IL-4) and Interleukin-13 (IL-13) Cytokines

(2013) Christoph Spiess et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Fcγ receptors enable anticancer action of proapoptotic and immune-modulatory antibodies

(2013) Jeong M. Kim et al.   JOURNAL OF EXPERIMENTAL MEDICINE

The structure of dual-variable-domain immunoglobulin molecules alone and bound to antigen

(2013) Ivan Correia et al.   mAbs

Structure reveals function of the dual variable domain immunoglobulin (DVD-Ig™) molecule

(2013) Clarissa G. Jakob et al.   mAbs

Bispecific antibodies with natural architecture produced by co-culture of bacteria expressing two distinct half-antibodies

(2013) Christoph Spiess et al.   NATURE BIOTECHNOLOGY

A novel bispecific EGFR/Met antibody blocks tumor-promoting phenotypic effects induced by resistance to EGFR inhibition and has potent antitumor activity

(2013) R Castoldi et al.   ONCOGENE

Aggregation and Chemical Modification of Monoclonal Antibodies under Upstream Processing Conditions

(2013) Stefan Dengl et al.   PHARMACEUTICAL RESEARCH

Identification and Multidimensional Optimization of an Asymmetric Bispecific IgG Antibody Mimicking the Function of Factor VIII Cofactor Activity

(2013) Zenjiro Sampei et al.   PLoS One

A Novel Angiopoietin-2 Selective Fully Human Antibody with Potent Anti-Tumoral and Anti-Angiogenic Efficacy and Superior Side Effect Profile Compared to Pan-Angiopoietin-1/-2 Inhibitors

(2013) Markus Thomas et al.   PLoS One

Crystal Structure of an Anti-Ang2 CrossFab Demonstrates Complete Structural and Functional Integrity of the Variable Domain

(2013) Sebastian Fenn et al.   PLoS One

Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange

(2013) A. F. Labrijn et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generating Bispecific Human IgG1 and IgG2 Antibodies from Any Antibody Pair

(2012) Pavel Strop et al.   JOURNAL OF MOLECULAR BIOLOGY

A novel bispecific antibody format enables simultaneous bivalent and monovalent co-engagement of distinct target antigens

(2012) Gregory L. Moore et al.   mAbs

Progress in overcoming the chain association issue in bispecific heterodimeric IgG antibodies

(2012) Christian Klein et al.   mAbs

A bispecific antibody to factors IXa and X restores factor VIII hemostatic activity in a hemophilia A model

(2012) Takehisa Kitazawa et al.   NATURE MEDICINE

Molecular characterization of novel trispecific ErbB-cMet-IGF1R antibodies and their antigen-binding properties

(2012) R. Castoldi et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Bispecific antibody derivatives with restricted binding functionalities that are activated by proteolytic processing

(2012) S. Metz et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A Two-in-One Antibody against HER3 and EGFR Has Superior Inhibitory Activity Compared with Monospecific Antibodies

(2011) Gabriele Schaefer et al.   CANCER CELL

A Human Neutralizing Antibody Specific to Ang-2 Inhibits Ocular Angiogenesis

(2011) EMMA S. RENNEL et al.   MICROCIRCULATION

Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies

(2011) W. Schaefer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhancing Antibody Fc Heterodimer Formation through Electrostatic Steering Effects

(2010) Kannan Gunasekaran et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

SEEDbodies: fusion proteins based on strand-exchange engineered domain (SEED) CH3 heterodimers in an Fc analogue platform for asymmetric binders or immunofusions and bispecific antibodies†

(2010) Jonathan H. Davis et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Variants of the Antibody Herceptin That Interact with HER2 and VEGF at the Antigen Binding Site

(2009) J. Bostrom et al.   SCIENCE