CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)

(2022) Guanying Wang et al.   BMC BIOLOGY

CRISPR/Cas9 gene editing and natural variation analysis demonstrate the potential for HvARE1 in improvement of nitrogen use efficiency in barley

(2022) Sakura D. Karunarathne et al.   Journal of Integrative Plant Biology

Genome-edited powdery mildew resistance in wheat without growth penalties

(2022) Shengnan Li et al.   NATURE

CRISPR /Cas genome‐editing toolkit to enhance salt stress tolerance in rice and wheat

(2022) Romaan Nazir et al.   PHYSIOLOGIA PLANTARUM

The rice heavy‐metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homoeostasis

(2022) Chuanliang Chu et al.   PLANT CELL AND ENVIRONMENT

CRISPR-Cas9 based stress tolerance: New hope for abiotic stress tolerance in chickpea (Cicer arietinum)

(2022) Muhammad Khuram Razzaq et al.   MOLECULAR BIOLOGY REPORTS

Xylella fastidiosa ’s relationships: the bacterium, the host plants, and the plant microbiome

(2022) Blanca B. Landa et al.   NEW PHYTOLOGIST

CRISPR-Cas9 Targeting of the eIF4E1 Gene Extends the Potato Virus Y Resistance Spectrum of the Solanum tuberosum L. cv. Desirée

(2022) Alessandra Lucioli et al.   Frontiers in Microbiology

Broad-spectrum resistance against multiple PVY-strains by CRSIPR/Cas13 system in Solanum tuberosum crop

(2022) Azka Noureen et al.   GM Crops & Food-Biotechnology in Agriculture and the Food Chain

CRISPR/Cas9 Mediated Knockout of the OsbHLH024 Transcription Factor Improves Salt Stress Resistance in Rice (Oryza sativa L.)

(2022) Mohammad Shah Alam et al.   Plants-Basel

CRISPR/Cas9-Mediated Targeting of Susceptibility Factor eIF4E-Enhanced Resistance Against Potato Virus Y

(2022) Azka Noureen et al.   Frontiers in Genetics

Highly Efficient Genome Editing Using Geminivirus-Based CRISPR/Cas9 System in Cotton Plant

(2022) Bo Li et al.   Cells

Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in Plants

(2022) Xiao Ding et al.   Cells

Plant Viruses: From Targets to Tools for CRISPR

(2021) Carla M. R. Varanda et al.   Viruses-Basel

Genome engineering for crop improvement and future agriculture

(2021) Caixia Gao   CELL

CRISPR/Cas9-Mediated Generation of Pathogen-Resistant Tomato against Tomato Yellow Leaf Curl Virus and Powdery Mildew

(2021) Dibyajyoti Pramanik et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Creation of aromatic maize by CRISPR/Cas

(2021) Yanxiao Wang et al.   Journal of Integrative Plant Biology

Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems

(2021) Yingxiao Zhang et al.   Nature Communications

Exosome/Liposome-like Nanoparticles: New Carriers for CRISPR Genome Editing in Plants

(2021) Mousa A. Alghuthaymi et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Increasing yield potential through manipulating of an ARE1 ortholog related to nitrogen use efficiency in wheat by CRISPR/Cas9

(2021) Jiahui Zhang et al.   Journal of Integrative Plant Biology

Loss of function of a DMR6 ortholog in tomato confers broad-spectrum disease resistance

(2021) Daniela Paula de Toledo Thomazella et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Towards novel herbicide modes of action by inhibiting lysine biosynthesis in plants

(2021) Tatiana P Soares da Costa et al.   eLife

Targeting of SPCSV‐ RNase3 via CRISPR‐Cas13 confers resistance against sweet potato virus disease

(2021) Yicheng Yu et al.   MOLECULAR PLANT PATHOLOGY

Engineered pegRNAs improve prime editing efficiency

(2021) James W. Nelson et al.   NATURE BIOTECHNOLOGY

Engineering of rice varieties with enhanced resistances to both blast and bacterial blight diseases via CRISPR/Cas9

(2021) Yanbiao Zhou et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9-mediated genome editing is revolutionizing the improvement of horticultural crops: Recent advances and future prospects

(2021) Harmeet Kaur et al.   SCIENTIA HORTICULTURAE

A Pathogen-Responsive Gene Cluster for Highly Modified Fatty Acids in Tomato

(2020) Ju Eun Jeon et al.   CELL

Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors

(2020) Chao Li et al.   NATURE BIOTECHNOLOGY

Targeted base editing in rice with CRISPR/ScCas9 system

(2020) Meixia Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Natural variation and CRISPR/Cas9‐mediated mutation in GmPRR37 affect photoperiodic flowering and contribute to regional adaptation of soybean

(2020) Liwei Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Rational Improvement of Rice Yield and Cold Tolerance by Editing the Three Genes OsPIN5b, GS3, and OsMYB30 With the CRISPR–Cas9 System

(2020) Yafei Zeng et al.   Frontiers in Plant Science

Down Regulation and Loss of Auxin Response Factor 4 Function Using CRISPR/Cas9 Alters Plant Growth, Stomatal Function and Improves Tomato Tolerance to Salinity and Osmotic Stress

(2020) Sarah Bouzroud et al.   Genes

CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects

(2020) Hakim Manghwar et al.   Advanced Science

Allelic Variants of CRISPR/Cas9 Induced Mutation in an Inositol Trisphosphate 5/6 Kinase Gene Manifest Different Phenotypes in Barley

(2020) Vlčko et al.   Plants-Basel

The evolving landscape around genome editing in agriculture

(2020) Sarah M Schmidt et al.   EMBO REPORTS

Genome editing with the CRISPR‐Cas system: an art, ethics and global regulatory perspective

(2020) Debin Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering herbicide resistance via prime editing in rice

(2020) Haroon Butt et al.   PLANT BIOTECHNOLOGY JOURNAL

The application of a heat‐inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton ( G.hirsutum ) plants

(2020) Qiongqiong Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Gene editing of three BnITPK genes in tetraploid oilseed rape leads to significant reduction of phytic acid in seeds

(2020) Niharika Sashidhar et al.   PLANT BIOTECHNOLOGY JOURNAL

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

(2020) Russell T. Walton et al.   SCIENCE

CRISPR-Cas9 mediated genome editing of drought and salt tolerance (OsDST) gene in indica mega rice cultivar MTU1010

(2020) V. V. Santosh Kumar et al.   PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS

CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew

(2020) Miguel I. Santillán Martínez et al.   BMC PLANT BIOLOGY

Targeted, efficient sequence insertion and replacement in rice

(2020) Yuming Lu et al.   NATURE BIOTECHNOLOGY

Review: amino acid biosynthesis as a target for herbicide development

(2020) Cody J Hall et al.   PEST MANAGEMENT SCIENCE

Fine‐tuning the amylose content of rice by precise base editing of the Wx gene

(2020) Yang Xu et al.   PLANT BIOTECHNOLOGY JOURNAL

Developing a novel artificial rice germplasm for dinitroaniline herbicide resistance by base editing of OsTubA2

(2020) Lang Liu et al.   PLANT BIOTECHNOLOGY JOURNAL

Base editing with high efficiency in allotetraploid oilseed rape by A3A‐PBE system

(2020) Hongtao Cheng et al.   PLANT BIOTECHNOLOGY JOURNAL

OsNRAMP1 transporter contributes to cadmium and manganese uptake in rice

(2020) Jia‐Dong Chang et al.   PLANT CELL AND ENVIRONMENT

The trihelix transcription factor OsGTγ-2 is involved adaption to salt stress in rice

(2020) Xiaoshuang Liu et al.   PLANT MOLECULAR BIOLOGY

CRISPR/Cas9-mediated mutagenesis of VvMLO3 results in enhanced resistance to powdery mildew in grapevine (Vitis vinifera)

(2020) Dong-Yan Wan et al.   Horticulture Research

Applications of CRISPR–Cas in agriculture and plant biotechnology

(2020) Haocheng Zhu et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton ( G. hirsutum ) and creation of nontransgenic, gossypol‐free cotton

(2020) Bo Li et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9-based precise excision of SlHyPRP1 domain(s) to obtain salt stress-tolerant tomato

(2020) Mil Thi Tran et al.   PLANT CELL REPORTS

Genome Edited Crops Touch the Market: A View on the Global Development and Regulatory Environment

(2020) Jochen Menz et al.   Frontiers in Plant Science

OsmiR535, a Potential Genetic Editing Target for Drought and Salinity Stress Tolerance in Oryza sativa

(2020) Erkui Yue et al.   Plants-Basel

Host susceptibility factors render ripe tomato fruit vulnerable to fungal disease despite active immune responses

(2020) Christian J Silva et al.   JOURNAL OF EXPERIMENTAL BOTANY

High‐oleic acid content, nontransgenic allotetraploid cotton ( Gossypium hirsutum L.) generated by knockout of GhFAD2 genes with CRISPR/Cas9 system

(2020) Yizhen Chen et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9 induces exon skipping that facilitates development of fragrant rice

(2020) Yongchao Tang et al.   PLANT BIOTECHNOLOGY JOURNAL

Metabolic Mechanisms of Host Species Against Citrus Huanglongbing (Greening Disease)

(2019) Muhammad Junaid Rao et al.   CRITICAL REVIEWS IN PLANT SCIENCES

Creating highly efficient resistance against Wheat dwarf virus in barley by employing CRISPR/Cas9 system

(2019) András Kis et al.   PLANT BIOTECHNOLOGY JOURNAL

Genetic Engineering for Disease Resistance in Plants: Recent Progress and Future Perspectives

(2019) Oliver Xiaoou Dong et al.   PLANT PHYSIOLOGY

Generation of virus-resistant potato plants by RNA genome targeting

(2019) Xiaohui Zhan et al.   PLANT BIOTECHNOLOGY JOURNAL

Mimicking natural polymorphism in eIF4E by CRISPR-Cas9 base editing is associated with resistance to potyviruses

(2019) Anna Bastet et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome editing for plant disease resistance: applications and perspectives

(2019) Kangquan Yin et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Enhanced rice salinity tolerance via CRISPR/Cas9-targeted mutagenesis of the OsRR22 gene

(2019) Anning Zhang et al.   MOLECULAR BREEDING

Generation of a new thermo-sensitive genic male sterile rice line by targeted mutagenesis of TMS5 gene through CRISPR/Cas9 system

(2019) Hirendra Nath Barman et al.   BMC PLANT BIOLOGY

CRISPR/Cas9-Mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance

(2019) Rui Li et al.   BMC PLANT BIOLOGY

Editing of Rice Isoamylase Gene ISA1 Provides Insights into Its Function in Starch Formation

(2019) Chao Shufen et al.   Rice Science

GM approaches to improve disease resistance in crops

(2019) H. Peter van Esse et al.   NEW PHYTOLOGIST

An adenine base editor with expanded targeting scope using SpCas9‐ NG v1 in rice

(2019) Katsuya Negishi et al.   PLANT BIOTECHNOLOGY JOURNAL

Cas9‐ PF , an early flowering and visual selection marker system, enhances the frequency of editing event occurrence and expedites the isolation of genome‐edited and transgene‐free plants

(2019) Yong Liu et al.   PLANT BIOTECHNOLOGY JOURNAL

Robust CRISPR /Cpf1(Cas12a) mediated genome editing in allotetraploid cotton ( G. hirsutum )

(2019) Bo Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Supersweet and waxy: meeting the diverse demands for specialty maize by genome editing

(2019) Le Dong et al.   PLANT BIOTECHNOLOGY JOURNAL

High Efficient and Precise Base Editing of C•G to T•A in the Allotetraploid Cotton ( Gossypium hirsutum ) Genome Using a Modified CRISPR /Cas9 System

(2019) Lei Qin et al.   PLANT BIOTECHNOLOGY JOURNAL

Improving plant-resistance to insect-pests and pathogens: The new opportunities through targeted genome editing

(2019) Deepak Singh Bisht et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

A New Rice Breeding Method: CRISPR /Cas9 System Editing of the Xa13 Promoter to Cultivate Transgene‐free Bacterial Blight‐resistant Rice

(2019) Changyan Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering Broad-Spectrum Bacterial Blight Resistance by Simultaneously Disrupting Variable TALE-binding Elements of Multiple Susceptibility Genes in Rice

(2019) Zhengyin Xu et al.   Molecular Plant

Knockout of two Bna MAX 1 homologs by CRISPR /Cas9‐targeted mutagenesis improves plant architecture and increases yield in rapeseed ( Brassica napus L.)

(2019) Ming Zheng et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9-mediated editing of CsWRKY22 reduces susceptibility to Xanthomonas citri subsp. citri in Wanjincheng orange (Citrus sinensis (L.) Osbeck)

(2019) Lijuan Wang et al.   Plant Biotechnology Reports

Citrus Genetic Engineering for Disease Resistance: Past, Present and Future

(2019) Lifang Sun et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Engineering Abiotic Stress Tolerance via CRISPR-Cas mediated genome editing

(2019) Syed Adeel Zafar et al.   JOURNAL OF EXPERIMENTAL BOTANY

Expanding the scope of CRISPR/Cas9‐mediated genome editing in plants using an xCas9 and Cas9‐NG hybrid

(2019) Qingfeng Niu et al.   Journal of Integrative Plant Biology

Broad-spectrum resistance to bacterial blight in rice using genome editing

(2019) Ricardo Oliva et al.   NATURE BIOTECHNOLOGY

Reduced fire blight susceptibility in apple cultivars using a high‐efficiency CRISPR/Cas9‐FLP/FRT‐based gene editing system

(2019) Valerio Pompili et al.   PLANT BIOTECHNOLOGY JOURNAL

PcMuORP1, an Oxathiapiprolin-Resistance Gene, Functions as a Novel Selection Marker for Phytophthora Transformation and CRISPR/Cas9 Mediated Genome Editing

(2019) Weizhen Wang et al.   Frontiers in Microbiology

CRISPR/Cas System: Recent Advances and Future Prospects for Genome Editing

(2019) Hakim Manghwar et al.   TRENDS IN PLANT SCIENCE

CRISPR Crops: Plant Genome Editing Toward Disease Resistance

(2018) Thorsten Langner et al.   Annual Review of Phytopathology

Phenotypic novelty by CRISPR in plants

(2018) Chen Liu et al.   DEVELOPMENTAL BIOLOGY

Engineering Introns to Express RNA Guides for Cas9- and Cpf1-Mediated Multiplex Genome Editing

(2018) Dan Ding et al.   Molecular Plant

Exploring and exploiting the boundaries of host specificity using the cereal rust and mildew models

(2018) Peter Michael Dracatos et al.   NEW PHYTOLOGIST

Trans-species synthetic gene design allows resistance pyramiding and broad-spectrum engineering of virus resistance in plants

(2018) Anna Bastet et al.   PLANT BIOTECHNOLOGY JOURNAL

Establishing RNA virus resistance in plants by harnessing CRISPR immune system

(2018) Tong Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus

(2018) Anca Macovei et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering herbicide-resistant watermelon variety through CRISPR/Cas9-mediated base-editing

(2018) Shouwei Tian et al.   PLANT CELL REPORTS

Mutations in a subfamily of abscisic acid receptor genes promote rice growth and productivity

(2018) Chunbo Miao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Transient Expression of CRISPR/Cas9 Machinery Targeting TcNPR3 Enhances Defense Response in Theobroma cacao

(2018) Andrew S. Fister et al.   Frontiers in Plant Science

Genome Editing and Double-Fluorescence Proteins Enable Robust Maternal Haploid Induction and Identification in Maize

(2018) Le Dong et al.   Molecular Plant

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Design of a bacterial speck resistant tomato by CRISPR/Cas9-mediated editing of SlJAZ2

(2018) Andrés Ortigosa et al.   PLANT BIOTECHNOLOGY JOURNAL

Directed evolution of CRISPR-Cas9 to increase its specificity

(2018) Jungjoon K. Lee et al.   Nature Communications

Risk of increased food insecurity under stringent global climate change mitigation policy

(2018) Tomoko Hasegawa et al.   Nature Climate Change

The Enhancement of Plant Disease Resistance Using CRISPR/Cas9 Technology

(2018) Virginia M. G. Borrelli et al.   Frontiers in Plant Science

Whole genome sequencing reveals rare off-target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR/Cas9-edited cotton plants

(2018) Jianying Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Research Progress on Heat Stress of Rice at Flowering Stage

(2018) Yaliang Wang et al.   Rice Science

Reduced Drought Tolerance by CRISPR/Cas9-Mediated SlMAPK3 Mutagenesis in Tomato Plants

(2017) Liu Wang et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Disruption of OsSEC3A increases the content of salicylic acid and induces plant defense responses in rice

(2017) Jin Ma et al.   JOURNAL OF EXPERIMENTAL BOTANY

Knock out of the annexin gene OsAnn3 via CRISPR/Cas9-mediated genome editing decreased cold tolerance in rice

(2017) Chunxiu Shen et al.   JOURNAL OF PLANT BIOLOGY

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

(2017) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

(2017) Zenpei Shimatani et al.   NATURE BIOTECHNOLOGY

Towards CRISPR/Cas crops - bringing together genomics and genome editing

(2017) Armin Scheben et al.   NEW PHYTOLOGIST

Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus

(2017) Aihong Peng et al.   PLANT BIOTECHNOLOGY JOURNAL

High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum ) using CRISPR/Cas9 system

(2017) Pengcheng Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

RNA-guided Cas9 as an in vivo desired-target mutator in maize

(2017) Chuxi Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system

(2017) Manuel Nieves-Cordones et al.   PLANT JOURNAL

Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat

(2017) Yunwei Zhang et al.   PLANT JOURNAL

Rice peroxisomal ascorbate peroxidase knockdown affects ROS signaling and triggers early leaf senescence

(2017) Carolina W. Ribeiro et al.   PLANT SCIENCE

RNA-guided transcriptional activation via CRISPR/dCas9 mimics overexpression phenotypes in Arabidopsis

(2017) Jong-Jin Park et al.   PLoS One

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

ROS Are Good

(2017) Ron Mittler   TRENDS IN PLANT SCIENCE

Knockout of OsNramp5 using the CRISPR/Cas9 system produces low Cd-accumulating indica rice without compromising yield

(2017) Li Tang et al.   Scientific Reports

OsSAPK2 Confers Abscisic Acid Sensitivity and Tolerance to Drought Stress in Rice

(2017) Dengji Lou et al.   Frontiers in Plant Science

Gene Editing and Crop Improvement Using CRISPR-Cas9 System

(2017) Leena Arora et al.   Frontiers in Plant Science

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

(2017) Vladimir Nekrasov et al.   Scientific Reports

Efficient targeted mutagenesis in soybean by TALENs and CRISPR/Cas9

(2016) Hongyang Du et al.   JOURNAL OF BIOTECHNOLOGY

Efficiency and Inheritance of Targeted Mutagenesis in Maize Using CRISPR-Cas9

(2016) Jinjie Zhu et al.   Journal of Genetics and Genomics

Efficient Targeted Genome Modification in Maize Using CRISPR/Cas9 System

(2016) Chao Feng et al.   Journal of Genetics and Genomics

CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications

(2016) Xingliang Ma et al.   Molecular Plant

Engineering Herbicide-Resistant Rice Plants through CRISPR/Cas9-Mediated Homologous Recombination of Acetolactate Synthase

(2016) Yongwei Sun et al.   Molecular Plant

Engineering of CRISPR/Cas9-mediated potyvirus resistance in transgene-freeArabidopsisplants

(2016) Douglas E. Pyott et al.   MOLECULAR PLANT PATHOLOGY

Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology

(2016) Jeyabharathy Chandrasekaran et al.   MOLECULAR PLANT PATHOLOGY

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

JAZ2 controls stomata dynamics during bacterial invasion

(2016) Selena Gimenez-Ibanez et al.   NEW PHYTOLOGIST

Tomato facultative parthenocarpy results from SlAGAMOUS-LIKE 6 loss of function

(2016) Chen Klap et al.   PLANT BIOTECHNOLOGY JOURNAL

ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under field drought stress conditions

(2016) Jinrui Shi et al.   PLANT BIOTECHNOLOGY JOURNAL

Consumer acceptance of food crops developed by genome editing

(2016) Tetsuya Ishii et al.   PLANT CELL REPORTS

The Arabidopsis UDP-glycosyltransferases UGT79B2 and UGT79B3, contribute to cold, salt and drought stress tolerance via modulating anthocyanin accumulation

(2016) Pan Li et al.   PLANT JOURNAL

A modular toolbox for gRNA–Cas9 genome engineering in plants based on the GoldenBraid standard

(2016) Marta Vazquez-Vilar et al.   Plant Methods

AMedicago truncatulaCystathionine-β-Synthase-like Domain-Containing Protein Is Required for Rhizobial Infection and Symbiotic Nitrogen Fixation

(2016) Senjuti Sinharoy et al.   PLANT PHYSIOLOGY

Status of the Archaeal and Bacterial Census: an Update

(2016) Patrick D. Schloss et al.   mBio

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

(2016) Sergei Svitashev et al.   Nature Communications

A Functional Bacterium-to-Plant DNA Transfer Machinery of Rhizobium etli

(2016) Benoît Lacroix et al.   PLoS Pathogens

Gene Inactivation by CRISPR-Cas9 in Nicotiana tabacum BY-2 Suspension Cells

(2016) Sébastien Mercx et al.   Frontiers in Plant Science

Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance

(2016) Syed Shan-e-Ali Zaidi et al.   Frontiers in Plant Science

DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins

(2016) Mickael Malnoy et al.   Frontiers in Plant Science

Knockdown of MLO genes reduces susceptibility to powdery mildew in grapevine

(2016) Stefano Pessina et al.   Horticulture Research

Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922

(2016) Fujun Wang et al.   PLoS One

An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design

(2016) Yongping Zhao et al.   Scientific Reports

CRISPR/Cas9-Mediated Immunity to Geminiviruses: Differential Interference and Evasion

(2016) Zahir Ali et al.   Scientific Reports

Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants

(2016) Yuriko Osakabe et al.   Scientific Reports

CRISPR/Cas9-mediated efficient and heritable targeted mutagenesis in tomato plants in the first and later generations

(2016) Changtian Pan et al.   Scientific Reports

CRISPR/Cas9-mediated mutagenesis of the RIN locus that regulates tomato fruit ripening

(2015) Yasuhiro Ito et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

The CRISPR/Cas9 system for plant genome editing and beyond

(2015) Luisa Bortesi et al.   BIOTECHNOLOGY ADVANCES

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9

(2015) Taeyoung Koo et al.   MOLECULES AND CELLS

Plant Virus Metagenomics: Advances in Virus Discovery

(2015) Marilyn J. Roossinck et al.   PHYTOPATHOLOGY

Modification of the PthA4 effector binding elements in Type I CsLOB1 promoter using Cas9/sgRNA to produce transgenic Duncan grapefruit alleviating XccΔpthA4:dCsLOB1.3 infection

(2015) Hongge Jia et al.   PLANT BIOTECHNOLOGY JOURNAL

Gene targeting by the TAL effector PthXo2 reveals cryptic resistance gene for bacterial blight of rice

(2015) Junhui Zhou et al.   PLANT JOURNAL

Highly efficient heritable plant genome engineering using Cas9 orthologues fromStreptococcus thermophilusandStaphylococcus aureus

(2015) Jeannette Steinert et al.   PLANT JOURNAL

Cas9-Guide RNA Directed Genome Editing in Soybean

(2015) Zhongsen Li et al.   PLANT PHYSIOLOGY

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

(2015) Levi G. Lowder et al.   PLANT PHYSIOLOGY

Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA

(2015) Sergei Svitashev et al.   PLANT PHYSIOLOGY

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Plant Translation Factors and Virus Resistance

(2015) Hélène Sanfaçon   Viruses-Basel

A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing

(2015) Kangquan Yin et al.   Scientific Reports

CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoirs

(2015) Yonggang Zhang et al.   Scientific Reports

Role of the Insect SupervectorsBemisia tabaciandFrankliniella occidentalisin the Emergence and Global Spread of Plant Viruses

(2015) Robert L. Gilbertson et al.   Annual Review of Virology

Establishing a CRISPR–Cas-like immune system conferring DNA virus resistance in plants

(2015) Xiang Ji et al.   Nature Plants

Conferring resistance to geminiviruses with the CRISPR–Cas prokaryotic immune system

(2015) Nicholas J. Baltes et al.   Nature Plants

Efficient CRISPR/Cas9-mediated Targeted Mutagenesis in Populus in the First Generation

(2015) Di Fan et al.   Scientific Reports

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

(2014) Hui-Li Xing et al.   BMC PLANT BIOLOGY

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

Genome editing in rice and wheat using the CRISPR/Cas system

(2014) Qiwei Shan et al.   Nature Protocols

Magical mystery tour: MLO proteins in plant immunity and beyond

(2014) Johanna Acevedo-Garcia et al.   NEW PHYTOLOGIST

Current state of herbicides in herbicide-resistant crops

(2014) Jerry M Green   PEST MANAGEMENT SCIENCE

CRISPR/Cas9-Mediated Targeted Mutagenesis in the Liverwort Marchantia polymorpha L.

(2014) Shigeo S. Sugano et al.   PLANT AND CELL PHYSIOLOGY

The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation

(2014) Hui Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

Xcc-facilitated agroinfiltration of citrus leaves: a tool for rapid functional analysis of transgenes in citrus leaves

(2014) Hongge Jia et al.   PLANT CELL REPORTS

The CRISPR/Cas system can be used as nuclease forin plantagene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny

(2014) Simon Schiml et al.   PLANT JOURNAL

Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering inArabidopsis thaliana

(2014) Friedrich Fauser et al.   PLANT JOURNAL

CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum

(2014) Junping Gao et al.   PLANT MOLECULAR BIOLOGY

Hairy Root Transformation Using Agrobacterium rhizogenes as a Tool for Exploring Cell Type-Specific Gene Expression and Function Using Tomato as a Model

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