Miniature CRISPR-Cas12 endonucleases – Programmed DNA targeting in a smaller package

Efficient Genome Editing by a Miniature CRISPR-AsCas12f1 Nuclease in Bacillus anthracis

(2022) Yanchun Wang et al.   Frontiers in Bioengineering and Biotechnology

Structural basis for substrate recognition and cleavage by the dimerization-dependent CRISPR–Cas12f nuclease

(2021) Renjian Xiao et al.   NUCLEIC ACIDS RESEARCH

Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing

(2021) Xiaoshu Xu et al.   MOLECULAR CELL

Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus

(2021) Do Yon Kim et al.   NATURE BIOTECHNOLOGY

Programmed genome editing by a miniature CRISPR-Cas12f nuclease

(2021) Zhaowei Wu et al.   Nature Chemical Biology

DNA interference states of the hypercompact CRISPR–CasΦ effector

(2021) Patrick Pausch et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3

(2021) Arturo Carabias et al.   Nature Communications

CRISPR-based diagnostics

(2021) Michael M. Kaminski et al.   Nature Biomedical Engineering

Clades of huge phages from across Earth’s ecosystems

(2020) Basem Al-Shayeb et al.   NATURE

CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects

(2020) Karthik Murugan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage

(2020) Tautvydas Karvelis et al.   NUCLEIC ACIDS RESEARCH

CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks

(2020) Joshua C Cofsky et al.   eLife

Structure of the miniature type V-F CRISPR-Cas effector enzyme

(2020) Satoru N. Takeda et al.   MOLECULAR CELL

Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a

(2019) Daan C. Swarts et al.   MOLECULAR CELL

The next generation of CRISPR–Cas technologies and applications

(2019) Adrian Pickar-Oliver et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA

(2018) Shi-Yuan Li et al.   CELL RESEARCH

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

(2018) Stefano Stella et al.   CELL

Functionally diverse type V CRISPR-Cas systems

(2018) Winston X. Yan et al.   SCIENCE

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a

(2017) Daan C. Swarts et al.   MOLECULAR CELL

The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit

(2017) Karthik Murugan et al.   MOLECULAR CELL

Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1

(2017) Takashi Yamano et al.   MOLECULAR CELL

Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage

(2017) Stefano Stella et al.   NATURE

Diversity and evolution of class 2 CRISPR–Cas systems

(2017) Sergey Shmakov et al.   NATURE REVIEWS MICROBIOLOGY

Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA

(2016) Takashi Yamano et al.   CELL

PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease

(2016) Hui Yang et al.   CELL

Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition

(2016) Pu Gao et al.   CELL RESEARCH

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

(2016) Ines Fonfara et al.   NATURE

The crystal structure of Cpf1 in complex with CRISPR RNA

(2016) De Dong et al.   NATURE

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

CRISPR-Cas immunity in prokaryotes

(2015) Luciano A. Marraffini   NATURE

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

(2011) E. Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex

(2009) Caryn R. Hale et al.   CELL

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

Effect of Genome Size on AAV Vector Packaging

(2009) Zhijian Wu et al.   MOLECULAR THERAPY

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE