Engineered disulfide bonds improve thermostability and activity of L‐isoleucine hydroxylase for efficient 4‐HIL production in Bacillus subtilis 168

Enhancement of substrate supply and ido expression to improve 4-hydroxyisoleucine production in recombinant Corynebacterium glutamicum ssp. lactofermentum

(2019) Feng Shi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Directed evolution and site-specific mutagenesis of l-isoleucine dioxygenase derived from Bacillus weihenstephanensis

(2018) Sen Huang et al.   BIOTECHNOLOGY LETTERS

Improving the Thermostability and Catalytic Efficiency of the Subunit-Fused Nitrile Hydratase by Semi-Rational Engineering

(2018) Yuanyuan Xia et al.   ChemCatChem

Sufficient NADPH supply and pknG deletion improve 4-hydroxyisoleucine production in recombinant Corynebacterium glutamicum

(2018) Feng Shi et al.   ENZYME AND MICROBIAL TECHNOLOGY

High production of 4-hydroxyisoleucine in Corynebacterium glutamicum by multistep metabolic engineering

(2018) Chenglin Zhang et al.   METABOLIC ENGINEERING

Coupled molecular dynamics mediate long- and short-range epistasis between mutations that affect stability and aggregation kinetics

(2018) Haoran Yu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A strategy for L-isoleucine dioxygenase screening and 4-hydroxyisoleucine production by resting cells

(2017) Chenglin Zhang et al.   Bioengineered

Elimination of a Free Cysteine by Creation of a Disulfide Bond Increases the Activity and Stability of Candida boidinii Formate Dehydrogenase

(2016) Junxian Zheng et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Overexpression of ppc and lysC to improve the production of 4-hydroxyisoleucine and its precursor l-isoleucine in recombinant Corynebacterium glutamicum ssp. lactofermentum

(2016) Feng Shi et al.   ENZYME AND MICROBIAL TECHNOLOGY

Efficient testosterone production by engineered Pichia pastoris co-expressing human 17β-hydroxysteroid dehydrogenase type 3 and Saccharomyces cerevisiae glucose 6-phosphate dehydrogenase with NADPH regeneration

(2016) Minglong Shao et al.   GREEN CHEMISTRY

4-Hydroxyisoleucine production of recombinant Corynebacterium glutamicum ssp. lactofermentum under optimal corn steep liquor limitation

(2015) Feng Shi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The Phyre2 web portal for protein modeling, prediction and analysis

(2015) Lawrence A Kelley et al.   Nature Protocols

Efficient Whole-Cell Biocatalyst for Acetoin Production with NAD+ Regeneration System through Homologous Co-Expression of 2,3-Butanediol Dehydrogenase and NADH Oxidase in Engineered Bacillus subtilis

(2014) Teng Bao et al.   PLoS One

Modification of E. coli central metabolism to optimize the biotransformation of L-isoleucine into 4-hydroxyisoleucine by enzymatic hydroxylation

(2012) A. D. Kivero et al.   APPLIED BIOCHEMISTRY AND MICROBIOLOGY

Improved thermal performance of Thermomyces lanuginosus GH11 xylanase by engineering of an N-terminal disulfide bridge

(2012) Yawei Wang et al.   BIORESOURCE TECHNOLOGY

Engineering a Disulfide Bond in the Lid Hinge Region of Rhizopus chinensis Lipase: Increased Thermostability and Altered Acyl Chain Length Specificity

(2012) Xiao-Wei Yu et al.   PLoS One

1,3-Dipolar cycloaddition of a chiral nitrone to (E)-1,4-dichloro-2-butene: a new efficient synthesis of (2S,3S,4R)-4-hydroxyisoleucine

(2012) Kaïss Aouadi et al.   TETRAHEDRON LETTERS

Development of thermostable Candida antarctica lipase B through novel in silico design of disulfide bridge

(2011) Quang Anh Tuan Le et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolic engineering of Escherichia coli to produce (2S, 3R, 4S)-4-hydroxyisoleucine

(2010) Sergey V. Smirnov et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Role of conformational sampling in computing mutation-induced changes in protein structure and stability

(2010) Elizabeth H. Kellogg et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

α-Amylase: An Ideal Representative of Thermostable Enzymes

(2009) Om Prakash et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Improving the thermostability and activity of Melanocarpus albomyces cellobiohydrolase Cel7B

(2009) Sanni P. Voutilainen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A novel l-isoleucine hydroxylating enzyme, l-isoleucine dioxygenase from Bacillus thuringiensis, produces (2S,3R,4S)-4-hydroxyisoleucine

(2009) Tomohiro Kodera et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Bacillus subtilisandB. mojavensisstrains connected to food poisoning produce the heat stable toxin amylosin

(2009) C. Apetroaie-Constantin et al.   JOURNAL OF APPLIED MICROBIOLOGY

Bacillus subtilis as potential producer for polyhydroxyalkanoates

(2009) Mamtesh Singh et al.   Microbial Cell Factories

Expression of Talaromyces emersonii cellobiohydrolase Cel7A in Saccharomyces cerevisiae and rational mutagenesis to improve its thermostability and activity

(2009) S. P. Voutilainen et al.   PROTEIN ENGINEERING DESIGN & SELECTION

GROMACS 4:  Algorithms for Highly Efficient, Load-Balanced, and Scalable Molecular Simulation

(2008) Berk Hess et al.   Journal of Chemical Theory and Computation

Protein secondary structure analyses from circular dichroism spectroscopy: Methods and reference databases

(2007) Lee Whitmore et al.   BIOPOLYMERS