Lysosomal GPCR-like protein LYCHOS signals cholesterol sufficiency to mTORC1

A structure of human Scap bound to Insig-2 suggests how their interaction is regulated by sterols

(2021) Renhong Yan et al.   SCIENCE

Validation of Trifluoromethylphenyl Diazirine Cholesterol Analogues As Cholesterol Mimetics and Photolabeling Reagents

(2021) Kathiresan Krishnan et al.   ACS Chemical Biology

mTOR at the nexus of nutrition, growth, ageing and disease

(2020) Grace Y. Liu et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Inter-domain dynamics drive cholesterol transport by NPC1 and NPC1L1 proteins

(2020) Piyali Saha et al.   eLife

Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2

(2020) Hongwu Qian et al.   CELL

The Lysosome at the Intersection of Cellular Growth and Destruction

(2020) Hijai R. Shin et al.   DEVELOPMENTAL CELL

Sterols in an intramolecular channel of Smoothened mediate Hedgehog signaling

(2020) Xiaofeng Qi et al.   Nature Chemical Biology

Structural mechanism for amino acid-dependent Rag GTPase nucleotide state switching by SLC38A9

(2020) Simon A. Fromm et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C

(2020) Oliver B. Davis et al.   DEVELOPMENTAL CELL

Multiple functional neurosteroid binding sites on GABAA receptors

(2019) Zi-Wei Chen et al.   PLOS BIOLOGY

Cryo-EM structure of oxysterol-bound human Smoothened coupled to a heterotrimeric Gi

(2019) Xiaofeng Qi et al.   NATURE

Smoothened stimulation by membrane sterols drives Hedgehog pathway activity

(2019) Ishan Deshpande et al.   NATURE

ER–lysosome contacts enable cholesterol sensing by mTORC1 and drive aberrant growth signalling in Niemann–Pick type C

(2019) Chun-Yan Lim et al.   NATURE CELL BIOLOGY

Even Cancer Cells Watch Their Cholesterol!

(2019) Romain Riscal et al.   MOLECULAR CELL

Architecture of human Rag GTPase heterodimers and their complex with mTORC1

(2019) Madhanagopal Anandapadamanaban et al.   SCIENCE

Structural basis for the docking of mTORC1 on the lysosomal surface

(2019) Kacper B. Rogala et al.   SCIENCE

Structural mechanism of a Rag GTPase activation checkpoint by the lysosomal folliculin complex

(2019) Rosalie E. Lawrence et al.   SCIENCE

Mechanisms and regulation of cholesterol homeostasis

(2019) Jie Luo et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structural Basis of Smoothened Activation in Hedgehog Signaling

(2018) Pengxiang Huang et al.   CELL

NUFIP1 is a ribosome receptor for starvation-induced ribophagy

(2018) Gregory A. Wyant et al.   SCIENCE

Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex

(2018) Xiaofeng Qi et al.   SCIENCE

Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched

(2018) Yunxiao Zhang et al.   CELL

Double-slit photoelectron interference in strong-field ionization of the neon dimer

(2018) Maksim Kunitski et al.   Nature Communications

Photoaffinity labeling with cholesterol analogues precisely maps a cholesterol-binding site in voltage-dependent anion channel-1

(2017) Melissa M. Budelier et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Common structural features of cholesterol binding sites in crystallized soluble proteins

(2017) Anna N. Bukiya et al.   JOURNAL OF LIPID RESEARCH

Cholesterol Modification of Smoothened Is Required for Hedgehog Signaling

(2017) Xu Xiao et al.   MOLECULAR CELL

Hybrid Structure of the RagA/C-Ragulator mTORC1 Activation Complex

(2017) Ming-Yuan Su et al.   MOLECULAR CELL

KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1

(2017) Rachel L. Wolfson et al.   NATURE

Lysosomal cholesterol activates mTORC1 via an SLC38A9–Niemann-Pick C1 signaling complex

(2017) Brian M. Castellano et al.   SCIENCE

Cellular Cholesterol Directly Activates Smoothened in Hedgehog Signaling

(2016) Pengxiang Huang et al.   CELL

Global, quantitative and dynamic mapping of protein subcellular localization

(2016) Daniel N Itzhak et al.   eLife

GOplot: an R package for visually combining expression data with functional analysis: Fig. 1.

(2015) Wencke Walter et al.   BIOINFORMATICS

Nutrient-Sensing Mechanisms across Evolution

(2015) Lynne Chantranupong et al.   CELL

Complementary probes reveal that phosphatidylserine is required for the proper transbilayer distribution of cholesterol

(2015) M. Maekawa et al.   JOURNAL OF CELL SCIENCE

SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1

(2015) Manuele Rebsamen et al.   NATURE

Lysosomal amino acid transporter SLC38A9 signals arginine sufficiency to mTORC1

(2015) S. Wang et al.   SCIENCE

Three pools of plasma membrane cholesterol and their relation to cholesterol homeostasis

(2014) Akash Das et al.   eLife

Direct imaging reveals stable, micrometer-scale lipid domains that segregate proteins in live cells

(2013) Alexandre Toulmay et al.   JOURNAL OF CELL BIOLOGY

An Extended Proteome Map of the Lysosomal Membrane Reveals Novel Potential Transporters

(2013) Agnès Chapel et al.   MOLECULAR & CELLULAR PROTEOMICS

Ether lipid generating enzyme AGPS alters the balance of structural and signaling lipids to fuel cancer pathogenicity

(2013) D. I. Benjamin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Tumor Suppressor Complex with GAP Activity for the Rag GTPases That Signal Amino Acid Sufficiency to mTORC1

(2013) L. Bar-Peled et al.   SCIENCE

Amino Acid Deprivation Inhibits TORC1 Through a GTPase-Activating Protein Complex for the Rag Family GTPase Gtr1

(2013) N. Panchaud et al.   Science Signaling

Ragulator-Rag Complex Targets mTORC1 to the Lysosomal Surface and Is Necessary for Its Activation by Amino Acids

(2010) Yasemin Sancak et al.   CELL

Activation of a Metabolic Gene Regulatory Network Downstream of mTOR Complex 1

(2010) Katrin Düvel et al.   MOLECULAR CELL

Structure of N-Terminal Domain of NPC1 Reveals Distinct Subdomains for Binding and Transfer of Cholesterol

(2009) Hyock Joo Kwon et al.   CELL

Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources

(2009) Da Wei Huang et al.   Nature Protocols

Switch-like Control of SREBP-2 Transport Triggered by Small Changes in ER Cholesterol: A Delicate Balance

(2008) Arun Radhakrishnan et al.   Cell Metabolism

Regulation of TORC1 by Rag GTPases in nutrient response

(2008) Eunjung Kim et al.   NATURE CELL BIOLOGY

The Rag GTPases Bind Raptor and Mediate Amino Acid Signaling to mTORC1

(2008) Y. Sancak et al.   SCIENCE

A Specific Cholesterol Binding Site Is Established by the 2.8 Å Structure of the Human β2-Adrenergic Receptor

(2008) Michael A. Hanson et al.   STRUCTURE