Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris

Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms

(2022) Jucan Gao et al.   ACS Synthetic Biology

Recombination machinery engineering for precise genome editing in methylotrophic yeast Ogataea polymorpha

(2021) Jiaoqi Gao et al.   iScience

Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris

(2021) Peng Cai et al.   NUCLEIC ACIDS RESEARCH

Dual Regulation of Cytoplasm and Peroxisomes for Improved Α-Farnesene Production in Recombinant Pichia pastoris

(2021) Hui Liu et al.   ACS Synthetic Biology

Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

(2021) Jucan Gao et al.   Synthetic and Systems Biotechnology

Screening neutral sites for metabolic engineering of methylotrophic yeast Ogataea polymorpha

(2021) Wei Yu et al.   Synthetic and Systems Biotechnology

A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris

(2021) Xihao Liao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A repackaged CRISPR platform increases homology-directed repair for yeast engineering

(2021) Deon Ploessl et al.   Nature Chemical Biology

Pichia pastoris : A highly successful expression system for optimal synthesis of heterologous proteins

(2020) Mohsen Karbalaei et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Metabolic engineering of Pichia pastoris for malic acid production from methanol

(2020) Feng Guo et al.   BIOTECHNOLOGY AND BIOENGINEERING

Dynamic Processing of Displacement Loops during Recombinational DNA Repair

(2019) Aurèle Piazza et al.   MOLECULAR CELL

CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

(2019) Qi Liu et al.   Microbial Cell Factories

Production of lycopene by metabolically engineered Pichia pastoris

(2019) Xinying Zhang et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Advances in genome editing through control of DNA repair pathways

(2019) Charles D. Yeh et al.   NATURE CELL BIOLOGY

Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii

(2019) Neil C. Dalvie et al.   ACS Synthetic Biology

Multi-Invasion-Induced Rearrangements as a Pathway for Physiological and Pathological Recombination

(2018) Aurèle Piazza et al.   BIOESSAYS

Metabolic engineering of Pichia pastoris

(2018) David A. Peña et al.   METABOLIC ENGINEERING

Extraction of genomic DNA from yeasts for PCR-based applications

(2018) Marko Lõoke et al.   BIOTECHNIQUES

Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis

(2018) Chuixing Kong et al.   Scientific Reports

Barriers and opportunities in bio-based production of hydrocarbons

(2018) Yongjin J. Zhou et al.   Nature Energy

Enhancing CRISPR/Cas9-mediated homology-directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52

(2017) Simin Shao et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

(2017) Astrid Weninger et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

Repair Pathway Choices and Consequences at the Double-Strand Break

(2016) Raphael Ceccaldi et al.   TRENDS IN CELL BIOLOGY

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One