Review
Chemistry, Multidisciplinary
Abid Hussain, Haiyin Yang, Mengjie Zhang, Qing Liu, Ghallab Alotaibi, Muhammad Irfan, Huining He, Jin Chang, Xing-Jie Liang, Yuhua Weng, Yuanyu Huang
Summary: This review elaborately discusses the impact of the COVID-19 pandemic and the progress of mRNA-based vaccines. While the development of mRNA vaccines is a significant accomplishment, widespread production and global availability remain a challenge.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Chemistry, Multidisciplinary
Kepan Chen, Na Fan, Hai Huang, Xin Jiang, Shugang Qin, Wen Xiao, Qian Zheng, Yupei Zhang, Xing Duan, Zeyi Qin, Yongmei Liu, Jun Zeng, Yuquan Wei, Xiangrong Song
Summary: In this study, researchers developed a new mRNA vaccine carrier called 4N4T-LNPs. Vaccines constructed using 4N4T-LNPs showed improved translation efficiency, antibody titers, and neutralizing antibody titers compared to approved vaccines. Additionally, the study found that the chemical structure of ionizable lipids can influence the types of neutralizing antibodies induced by mRNA vaccines.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Mae M. Lewis, Melissa R. Soto, Esther Y. Maier, Steven D. Wulfe, Sandy Bakheet, Hannah Obregon, Debadyuti Ghosh
Summary: Although mRNA lipid nanoparticles (LNPs) have proven effective as vaccines, their efficacy for pulmonary delivery had not been fully confirmed. The instability of LNPs during aerosolization hindered their local delivery, resulting in mRNA cargo degradation and reduced cell transfection. In this study, we formulated mRNA LNPs with SM-102, a clinically approved ionizable lipid, which showed promising results for aerosolized pulmonary delivery.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Biotechnology & Applied Microbiology
Gabor Tamas Szabo, Azita Josefine Mahiny, Irena Vlatkovic
Summary: Since the discovery of the advantages of mRNA vaccines nearly 30 years ago, significant progress has been made in this field. The development of COVID-19 vaccines using mRNA technology has achieved unprecedented success globally, demonstrating the safety and effectiveness of mRNA vaccines in combating COVID-19 and highlighting their potential for other infectious diseases and medical conditions.
Review
Pharmacology & Pharmacy
Linde Schoenmaker, Dominik Witzigmann, Jayesh A. Kulkarni, Rein Verbeke, Gideon Kersten, Wim Jiskoot, Daan J. A. Crommelin
Summary: The current mRNA-lipid nanoparticle COVID-19 vaccines require storage at low temperatures due to instability. Improving mRNA nucleotide composition, understanding the impact of mRNA environment within LNPs, and exploring drying techniques can enhance vaccine stability.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Wei Wang, Shuo Feng, Zhuyifan Ye, Hanlu Gao, Jinzhong Lin, Defang Ouyang
Summary: The study utilized machine learning algorithms and molecular dynamic modeling to accelerate the development of mRNA vaccine LNPs, demonstrating the feasibility of the predictive model. Experimental validation confirmed the accuracy of the predictive model, providing a new approach for virtual screening of LNP formulations in the future.
ACTA PHARMACEUTICA SINICA B
(2022)
Review
Polymer Science
Ting Liu, Yang Tian, Aiping Zheng, Chunying Cui
Summary: This article reviews the design strategies of mRNA-LNP vaccines and the research strategies and potential development directions for COVID-19.
Article
Chemistry, Multidisciplinary
Rein Verbeke, Ine Lentacker, Stefaan C. De Smedt, Heleen Dewitte
Summary: In less than a year since the COVID-19 pandemic outbreak, mRNA vaccines have made significant progress, with two receiving emergency use authorization and one moving to phase 3 clinical testing. These mRNA vaccines represent a new class of vaccine products, encoding the SARS-CoV-2 Spike glycoprotein to deliver mRNA to cells. The review provides a detailed overview of the composition, clinical performance, and design considerations of the most advanced mRNA vaccines, highlighting new insights and areas for future research and optimization.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Allergy
Karim Dorgham, Paul Quentric, Mehmet Gokkaya, Stephane Marot, Christophe Parizot, Delphine Sauce, Amelie Guihot, Charles-Edouard Luyt, Matthieu Schmidt, Julien Mayaux, Alexandra Beurton, Loic Le Guennec, Sophie Demeret, Elyes Ben Salah, Alexis Mathian, Hans Yssel, Behazine Combadiere, Christophe Combadiere, Claudia Traidl-Hoffmann, Sonia Burrel, Anne-Genevieve Marcelin, Zahir Amoura, Guillaume Voiriot, Avidan U. Neumann, Guy Gorochov
Summary: Anaphylactic reactions to the novel mRNA lipid nanoparticle vaccines have raised significant public concern due to their unexpected occurrence, particularly among atopic individuals. Various potential contributors to these reactions have been considered, yet the clinical utility of available testing methods remains unclear. Further research and clinical trials are needed to address these concerns and ensure safety during public health emergencies.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yixiang Wang, Xiao Si, Yi Feng, Dan Feng, Xiaoyu Xu, Yan Zhang
Summary: Ionizable lipid-containing lipid nanoparticles (LNPs) are ideal delivery systems for gene therapy. Screening ionizable lipid libraries with diverse structures can provide new candidates for LNPs to deliver nucleic acid drugs. In this study, we prepared ionizable lipids containing the triazole moiety using click chemistry and demonstrated their potential for LNP assembly and mRNA delivery.
Article
Chemistry, Multidisciplinary
Zhongyu Li, Xue-Qing Zhang, William Ho, Xin Bai, Dabbu Kumar Jaijyan, Fengqiao Li, Ranjeet Kumar, Afsal Kolloli, Selvakumar Subbian, Hua Zhu, Xiaoyang Xu
Summary: A new class of lipid-modified polymer nanoparticles is developed for gene delivery, showing efficient delivery of both DNA and mRNA with the capability of long-term lyophilized storage.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Polymer Science
Akon Higuchi, Tzu-Cheng Sung, Ting Wang, Qing-Dong Ling, S. Suresh Kumar, Shih-Tien Hsu, Akihiro Umezawa
Summary: This review discusses the current usage of biomaterials in vaccine development, focusing on new platforms and mRNA vaccines. mRNA vaccines are considered safer than traditional vaccines but require special temperature conditions for storage and transportation. Further development is needed to improve the efficacy of mRNA vaccines.
Article
Allergy
Kimberly A. Risma, Kathryn M. Edwards, Donna S. Hummell, Frederic F. Little, Allison E. Norton, Amy Stallings, Robert A. Wood, Joshua D. Milner
Summary: The COVID-19 pandemic has led to the rapid production of messenger RNA vaccines packaged in lipid nanoparticles, resulting in unexpectedly severe allergic reactions. Various factors have been considered as possible contributors to these reactions, including nucleic acid activation of the contact system and preexisting antibody recognition of polyethylene glycol on the nanoparticle surface. Further research and clinical trials are needed to address concerns and determine the applicability of testing methods in predicting and understanding vaccine-related allergic reactions.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Xuexiang Han, Hanwen Zhang, Kamila Butowska, Kelsey L. Swingle, Mohamad-Gabriel Alameh, Drew Weissman, Michael J. Mitchell
Summary: In recent years, RNA therapeutics has seen significant growth, largely due to decades of research on lipid nanoparticles, particularly its key component - ionizable lipid. This commentary discusses the major types of ionizable lipids and offers perspectives for future development.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Aurelien vander Straeten, Morteza Sarmadi, John L. Daristotle, Maria Kanelli, Lisa H. Tostanoski, Joe Collins, Apurva Pardeshi, Jooli Han, Dhruv Varshney, Behnaz Eshaghi, Johnny Garcia, Timothy A. Forster, Gary Li, Nandita Menon, Sydney L. Pyon, Linzixuan Zhang, Catherine Jacob-Dolan, Olivia C. Powers, Kevin Hall, Shahad K. Alsaiari, Morris Wolf, Mark W. Tibbitt, Robert Farra, Dan H. Barouch, Robert Langer, Ana Jaklenec
Summary: Automated fabrication of microneedle patch mRNA vaccines for COVID-19 could improve vaccine accessibility by eliminating the need for a cold chain and trained healthcare personnel. The printing process of mRNA vaccines in a microneedle patch format is described, and the stability and efficiency of the vaccine are demonstrated. Additionally, immunizations using the microneedle patches stimulate long-term immune responses similar to intramuscular administration.
NATURE BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Xin Bai, Guolin Zhao, Qijing Chen, Zhongyu Li, Mingzhu Gao, William Ho, Xiaoyang Xu, Xue-Qing Zhang
Summary: In this study, an inhalable nanoparticle system incorporating siRNA against interleukin-11 (IL-11) was developed to inhibit fibroblast differentiation and reduce fibrosis development in idiopathic pulmonary fibrosis (IPF).
Article
Engineering, Biomedical
Bin Wang, Maoping Tang, Ziwei Yuan, Zhongyu Li, Bin Hu, Xin Bai, Jinxian Chu, Xiaoyang Xu, Xue-Qing Zhang
Summary: Immunotherapy is a powerful tool for combating human diseases, but its application in serious brain diseases is limited due to a lack of effective drug delivery technologies. In this study, researchers developed bioengineered ferritin nanoparticles that could transport a small-molecule immunomodulator across the blood-brain barrier and target specific cells in a glioma mouse model. These nanoparticles showed enhanced immunotherapeutic efficacy and resulted in tumor growth inhibition and prolonged survival. This biomimetic brain delivery platform offers new opportunities for brain drug delivery and immunotherapy in glioma patients.
BIOACTIVE MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Guolin Zhao, William Ho, Jinxian Chu, Xiaojian Xiong, Bin Hu, Kofi Oti Boakye-Yiadom, Xiaoyang Xu, Xue-Qing Zhang
Summary: A noninvasive aerosol inhalation nanoparticle system has been developed for the treatment of KRAS-mutant non-small-cell lung cancer (NSCLC). The inhalable nanoparticles show effective tumor targeting and enhanced antitumor activity in a mouse model. Compared to systemic delivery, the nebulized nanoparticles demonstrate better biosafety and present a promising gene therapy approach for KRAS-mutant NSCLC and other respiratory diseases.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Mingzhu Gao, Maoping Tang, William Ho, Yilong Teng, Qijing Chen, Lei Bu, Xiaoyang Xu, Xue-Qing Zhang
Summary: Atherosclerosis is a common pathology in cardiovascular diseases, and current therapies have limitations in reducing major adverse cardiovascular events. Modulating inflammation is an attractive avenue for treating atherosclerosis, but challenges in managing plaque inflammation have hindered progress. This study presents a nanoparticle-mediated mRNA therapeutic approach that successfully targets atherosclerotic plaques and modulates inflammation, showing potential for clinical applications.
Article
Chemistry, Multidisciplinary
Chenshuang Zhang, Yilong Teng, Fengqiao Li, William Ho, Xin Bai, Xiaoyang Xu, Xue-Qing Zhang
Summary: Chronic liver injury and inflammation caused by metabolic abnormalities trigger the activation of hepatic stellate cells (HSCs), leading to fibrosis and dysfunction in the liver. A nanoparticle-assisted RNA interfering approach was developed to target activated HSCs and block IL-11/ERK signaling, effectively regulating HSC transdifferentiation and fibrotic remodeling. The therapeutic efficacy of HSC-targeting nanoparticles encapsulating siRNA against IL-11 or IL11ra1 was demonstrated in resolving fibrosis and NASH.
Article
Multidisciplinary Sciences
Fengqiao Li, Xue-Qing Zhang, William Ho, Maoping Tang, Zhongyu Li, Lei Bu, Xiaoyang Xu
Summary: This study reports a single-agent mRNA nanomedicine-based strategy that utilizes mRNA lipid nanoparticles (LNPs) encoding the N-terminus of gasdermin to trigger pyroptosis, eliciting robust antitumor immunity and sensitizing tumors to anti-PD-1 immunotherapy. The strategy turns cold tumors into hot ones and suppresses the growth of distant tumors, offering opportunities for cancer treatment.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Ziwei Yuan, Bin Wang, Yilong Teng, William Ho, Bin Hu, Kofi Oti Boakye-Yiadom, Xiaoyang Xu, Xue-Qing Zhang
Summary: Gene therapy shows potential for treating incurable brain diseases. However, a challenge is the lack of effective gene delivery systems. This study developed modified self-assembled ferritin nanoparticles that can cross the blood-brain barrier and improve gene encapsulation, providing insights for future gene therapy.
