J-domain protein chaperone circuits in proteostasis and disease

Chemogenomic and bioinformatic profiling of ERdj paralogs underpins their unique roles in cancer

(2022) Laura E. Knighton et al.   CELL STRESS & CHAPERONES

Two distinct classes of co-chaperones compete for the EEVD motif in heat shock protein 70 (Hsp70) to tune its chaperone activities

(2022) Oleta T. Johnson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

NudC guides client transfer between the Hsp40/70 and Hsp90 chaperone systems

(2022) Maximilian M. Biebl et al.   MOLECULAR CELL

Chaperones directly and efficiently disperse stress-triggered biomolecular condensates

(2022) Haneul Yoo et al.   MOLECULAR CELL

Differential roles for DNAJ isoforms in HTT-polyQ and FUS aggregation modulation revealed by chaperone screens

(2022) Kinneret Rozales et al.   Nature Communications

Regulatory inter-domain interactions influence Hsp70 recruitment to the DnaJB8 chaperone

(2021) Bryan D. Ryder et al.   Nature Communications

The Hsp70 chaperone system: distinct roles in erythrocyte formation and maintenance

(2021) Yasith Mathangasinghe et al.   HAEMATOLOGICA

Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains

(2021) Benjamin L. Piette et al.   MOLECULAR CELL

On the evolution of chaperones and cochaperones and the expansion of proteomes across the Tree of Life

(2021) Mathieu E. Rebeaud et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The landscape of molecular chaperones across human tissues reveals a layered architecture of core and variable chaperones

(2021) Netta Shemesh et al.   Nature Communications

The TPR and J-domain containing proteins DJC31 and DJC62 are involved in abiotic stress response in Arabidopsis thaliana

(2021) Sophie Dittmer et al.   JOURNAL OF CELL SCIENCE

MBD5 and MBD6 couple DNA methylation to gene silencing through the J-domain protein SILENZIO

(2021) Lucia Ichino et al.   SCIENCE

AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models

(2021) Mihaly Varadi et al.   NUCLEIC ACIDS RESEARCH

Nematode CDC-37 and DNJ-13 form complexes and can interact with HSP-90

(2021) Lukas Schmauder et al.   Scientific Reports

Co-chaperone involvement in knob biogenesis implicates host-derived chaperones in malaria virulence

(2021) Mathias Diehl et al.   PLoS Pathogens

DNAJB6b-enriched small extracellular vesicles decrease polyglutamine aggregation in in vitro and in vivo models of Huntington disease

(2021) Bhagyashree S. Joshi et al.   iScience

Widespread remodeling of proteome solubility in response to different protein homeostasis stresses

(2020) Xiaojing Sui et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bacterial Hsp70 resolves misfolded states and accelerates productive folding of a multi-domain protein

(2020) Rahmi Imamoglu et al.   Nature Communications

Selective Binding of HSC70 and its Co-Chaperones to Structural Hotspots on CFTR

(2020) Imad Baaklini et al.   Scientific Reports

Biochemical Convergence of Mitochondrial Hsp70 System Specialized in Iron–Sulfur Cluster Biogenesis

(2020) Malgorzata Kleczewska et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Disassembly of Tau fibrils by the human Hsp70 disaggregation machinery generates small seeding-competent species

(2020) Eliana Nachman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

J-Domain Proteins in Bacteria and Their Viruses

(2020) Roland Barriot et al.   JOURNAL OF MOLECULAR BIOLOGY

Differentiation Drives Widespread Rewiring of the Neural Stem Cell Chaperone Network

(2020) Willianne I.M. Vonk et al.   MOLECULAR CELL

Two-step mechanism of J-domain action in driving Hsp70 function

(2020) Bartlomiej Tomiczek et al.   PLoS Computational Biology

A multi-omics analysis reveals the unfolded protein response regulon and stress-induced resistance to folate-based antimetabolites

(2020) Stefan Reich et al.   Nature Communications

Hsp40 proteins phase separate to chaperone the assembly and maintenance of membraneless organelles

(2020) Jinge Gu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The proteostasis network and its decline in ageing

(2019) Mark S. Hipp et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Hsp70- and Hsp90-Mediated Regulation of the Conformation of p53 DNA Binding Domain and p53 Cancer Variants

(2019) Marta Boysen et al.   MOLECULAR CELL

The Hsp70 chaperone network

(2019) Rina Rosenzweig et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Heat shock proteins create a signature to predict the clinical outcome in breast cancer

(2019) Marta Klimczak et al.   Scientific Reports

The Hsp70 Chaperone System Stabilizes a Thermo-sensitive Subproteome in E. coli

(2019) Liang Zhao et al.   Cell Reports

Structural basis for client recognition and activity of Hsp40 chaperones

(2019) Yajun Jiang et al.   SCIENCE

Unraveling the structure and dynamics of the human DNAJB6b chaperone by NMR reveals insights into Hsp40-mediated proteostasis

(2019) Theodoros K. Karamanos et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural and functional analysis of the Hsp70/Hsp40 chaperone system

(2019) Qinglian Liu et al.   PROTEIN SCIENCE

The expanding world of plant J-domain proteins

(2019) Amit K. Verma et al.   CRITICAL REVIEWS IN PLANT SCIENCES

Efficient conversion of chemical energy into mechanical work by Hsp70 chaperones

(2019) Salvatore Assenza et al.   eLife

The disorderly conduct of Hsc70 and its interaction with the Alzheimer's-related Tau protein

(2018) Isabelle R. Taylor et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Molecular Mechanism of J-Domain-Triggered ATP Hydrolysis by Hsp70 Chaperones

(2018) Roman Kityk et al.   MOLECULAR CELL

Chaperones convert the energy from ATP into the nonequilibrium stabilization of native proteins

(2018) Pierre Goloubinoff et al.   Nature Chemical Biology

Mapping interactions with the chaperone network reveals factors that protect against tau aggregation

(2018) Sue-Ann Mok et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Intra-molecular pathways of allosteric control in Hsp70s

(2018) Matthias P. Mayer   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Protein Disaggregation in Multicellular Organisms

(2018) Nadinath B. Nillegoda et al.   TRENDS IN BIOCHEMICAL SCIENCES

Mapping interactions with the chaperone network reveals factors that protect against tau aggregation

(2018) Sue-Ann Mok et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural modelling of the DNAJB6 oligomeric chaperone shows a peptide-binding cleft lined with conserved S/T-residues at the dimer interface

(2018) Christopher A. G. Söderberg et al.   Scientific Reports

Chaperone-Mediated Sec61 Channel Gating during ER Import of Small Precursor Proteins Overcomes Sec61 Inhibitor-Reinforced Energy Barrier

(2018) Sarah Haßdenteufel et al.   Cell Reports

Recent advances in the structural and mechanistic aspects of Hsp70 molecular chaperones

(2018) Matthias P Mayer et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Allosteric landscapes of eukaryotic cytoplasmic Hsp70s are shaped by evolutionary tuning of key interfaces

(2018) Wenli Meng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Novel DNAJ-related proteins in Arabidopsis thaliana

(2017) Pablo Pulido et al.   NEW PHYTOLOGIST

Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes

(2017) Nadinath B Nillegoda et al.   eLife

Modeling Hsp70/Hsp40 interaction by multi-scale molecular simulations and coevolutionary sequence analysis

(2017) Duccio Malinverni et al.   eLife

Members of the Hsp70 Family Recognize Distinct Types of Sequences to Execute ER Quality Control

(2016) Julia Behnke et al.   MOLECULAR CELL

Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation

(2016) Rui Sousa et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Phosphorylation of Cysteine String Protein Triggers a Major Conformational Switch

(2016) Pryank Patel et al.   STRUCTURE

Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation

(2016) Rui Sousa et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The Biology of Proteostasis in Aging and Disease

(2015) Johnathan Labbadia et al.   Annual Review of Biochemistry

Widespread Proteome Remodeling and Aggregation in Aging C. elegans

(2015) Dirk M. Walther et al.   CELL

Defining Hsp70 Subnetworks in Dengue Virus Replication Reveals Key Vulnerability in Flavivirus Infection

(2015) Shuhei Taguwa et al.   CELL

Quantitative proteomics of heat-treated human cells show an across-the-board mild depletion of housekeeping proteins to massively accumulate few HSPs

(2015) Andrija Finka et al.   CELL STRESS & CHAPERONES

Functionality of Class A and Class B J-protein co-chaperones with Hsp70

(2015) Hyun Young Yu et al.   FEBS LETTERS

Roles of Intramolecular and Intermolecular Interactions in Functional Regulation of the Hsp70 J-protein Co-Chaperone Sis1

(2015) Hyun Young Yu et al.   JOURNAL OF MOLECULAR BIOLOGY

Iron–Sulfur Cluster Biogenesis Chaperones: Evidence for Emergence of Mutational Robustness of a Highly Specific Protein–Protein Interaction

(2015) Wojciech Delewski et al.   MOLECULAR BIOLOGY AND EVOLUTION

Crucial HSP70 co-chaperone complex unlocks metazoan protein disaggregation

(2015) Nadinath B. Nillegoda et al.   NATURE

DNAJB3/HSP-40 cochaperone improves insulin signaling and enhances glucose uptake in vitro through JNK repression

(2015) Mohamed Abu-Farha et al.   Scientific Reports

Structure of CbpA J-Domain Bound to the Regulatory Protein CbpM Explains Its Specificity and Suggests Evolutionary Link between CbpM and Transcriptional Regulators

(2014) Naghmeh S. Sarraf et al.   PLoS One

Single-molecule spectroscopy reveals chaperone-mediated expansion of substrate protein

(2014) Ruth Kellner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hsp70 chaperones are non-equilibrium machines that achieve ultra-affinity by energy consumption

(2014) Paolo De Los Rios et al.   eLife

Proteomic data from human cell cultures refine mechanisms of chaperone-mediated protein homeostasis

(2013) Andrija Finka et al.   CELL STRESS & CHAPERONES

Phosphorylation of heat shock protein 40 (Hsp40/DnaJB1) by mitogen-activated protein kinase-activated protein kinase 5 (MK5/PRAK)

(2013) Sergiy Kostenko et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

Structural Insights into the Chaperone Activity of the 40-kDa Heat Shock Protein DnaJ

(2013) Jorge Cuéllar et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Binding of the Chaperone Jac1 Protein and Cysteine Desulfurase Nfs1 to the Iron-Sulfur Cluster Scaffold Isu Protein Is Mutually Exclusive

(2013) Julia Majewska et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The DNAJA2 Substrate Release Mechanism Is Essential for Chaperone-mediated Folding

(2012) Imad Baaklini et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Identification of Regions Involved in Substrate Binding and Dimer Stabilization within the Central Domains of Yeast Hsp40 Sis1

(2012) Júlio C. Borges et al.   PLoS One

A Bacteriophage-Encoded J-Domain Protein Interacts with the DnaK/Hsp70 Chaperone and Stabilizes the Heat-Shock Factor σ32 of Escherichia coli

(2012) Elsa Perrody et al.   PLoS Genetics

Human DNAJ in cancer and stem cells

(2011) Jason N. Sterrenberg et al.   CANCER LETTERS

Heat shock protein 70 kDa chaperone/DnaJ cochaperone complex employs an unusual dynamic interface

(2011) A. Ahmad et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Binding of a Small Molecule at a Protein–Protein Interface Regulates the Chaperone Activity of Hsp70–Hsp40

(2010) Susanne Wisén et al.   ACS Chemical Biology

Mutagenesis Reveals the Complex Relationships between ATPase Rate and the Chaperone Activities ofEscherichia coliHeat Shock Protein 70 (Hsp70/DnaK)

(2010) Lyra Chang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural Basis of the Regulation of the CbpA Co-chaperone by its Specific Modulator CbpM

(2010) Naghmeh S. Sarraf et al.   JOURNAL OF MOLECULAR BIOLOGY

The HSP70 chaperone machinery: J proteins as drivers of functional specificity

(2010) Harm H. Kampinga et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structure of clathrin coat with bound Hsc70 and auxilin: mechanism of Hsc70-facilitated disassembly

(2009) Yi Xing et al.   EMBO JOURNAL

The Mammalian Hsp40 ERdj3 Requires Its Hsp70 Interaction and Substrate-binding Properties to Complement Various Yeast Hsp40-dependent Functions

(2009) Shruthi S. Vembar et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cwc23, an Essential J Protein Critical for Pre-mRNA Splicing with a Dispensable J Domain

(2009) C. Sahi et al.   MOLECULAR AND CELLULAR BIOLOGY

Molecular Basis for Regulation of the Heat Shock Transcription Factor σ32 by the DnaK and DnaJ Chaperones

(2008) Fernanda Rodriguez et al.   MOLECULAR CELL