Type III-A CRISPR systems as a versatile gene knockdown technology

Structural and biochemical characterization of in vivo assembled Lactococcus lactis CRISPR-Csm complex

(2022) Sagar Sridhara et al.   Communications Biology

Programmable RNA targeting with the single-protein CRISPR effector Cas7-11

(2021) Ahsen Özcan et al.   NATURE

The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with a caspase-like peptidase

(2021) Sam P. B. van Beljouw et al.   SCIENCE

SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation

(2021) Jurre A. Steens et al.   Nature Communications

New Type III CRISPR variant and programmable RNA targeting tool: Oh, thank heaven for Cas7-11

(2021) Ryan J. Catchpole et al.   MOLECULAR CELL

Virus detection via programmable Type III-A CRISPR-Cas systems

(2021) Sagar Sridhara et al.   Nature Communications

RNA-targeting CRISPR systems from metagenomic discovery to transcriptomic engineering

(2020) Aaron A. Smargon et al.   NATURE CELL BIOLOGY

Generation of pre-tRNAs from polycistronic operons is the essential function of RNase P in Escherichia coli

(2020) Bijoy K Mohanty et al.   NUCLEIC ACIDS RESEARCH

Massively parallel Cas13 screens reveal principles for guide RNA design

(2020) Hans-Hermann Wessels et al.   NATURE BIOTECHNOLOGY

Regulation of the RNA and DNA nuclease activities required for Pyrococcus furiosus Type III-B CRISPR–Cas immunity

(2020) Kawanda Foster et al.   NUCLEIC ACIDS RESEARCH

Characterization of a novel type III CRISPR-Cas effector provides new insights into the allosteric activation and suppression of the Cas10 DNase

(2020) Jinzhong Lin et al.   Cell Discovery

Targeted RNA Knockdown by a Type III CRISPR-Cas Complex in Zebrafish

(2020) Thomas Fricke et al.   CRISPR Journal

Non-specific degradation of transcripts promotes plasmid clearance during type III-A CRISPR–Cas immunity

(2019) Jakob T. Rostøl et al.   Nature Microbiology

Genetic Dissection of the Type III-A CRISPR-Cas System Csm Complex Reveals Roles of Individual Subunits

(2019) Irmantas Mogila et al.   Cell Reports

Structural organization of a Type III-A CRISPR effector subcomplex determined by X-ray crystallography and cryo-EM

(2019) Bryan W Dorsey et al.   NUCLEIC ACIDS RESEARCH

The current state and future directions of RNAi-based therapeutics

(2019) Ryan L. Setten et al.   NATURE REVIEWS DRUG DISCOVERY

Cas13-induced cellular dormancy prevents the rise of CRISPR-resistant bacteriophage

(2019) Alexander J. Meeske et al.   NATURE

The next generation of CRISPR–Cas technologies and applications

(2019) Adrian Pickar-Oliver et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Second Messenger cA4 Formation within the Composite Csm1 Palm Pocket of Type III-A CRISPR-Cas Csm Complex and Its Release Path

(2019) Ning Jia et al.   MOLECULAR CELL

Cmr3 regulates the suppression on cyclic oligoadenylate synthesis by tag complementarity in a Type III-B CRISPR-Cas system

(2019) Tong Guo et al.   RNA Biology

Targeted transcriptional modulation with type I CRISPR–Cas systems in human cells

(2019) Adrian Pickar-Oliver et al.   NATURE BIOTECHNOLOGY

Maintaining Integrity Under Stress: Envelope Stress Response Regulation of Pathogenesis in Gram-Negative Bacteria

(2019) Claire L. Hews et al.   Frontiers in Cellular and Infection Microbiology

Harnessing type I CRISPR–Cas systems for genome engineering in human cells

(2019) Peter Cameron et al.   NATURE BIOTECHNOLOGY

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Type IV CRISPR–Cas systems are highly diverse and involved in competition between plasmids

(2019) Rafael Pinilla-Redondo et al.   NUCLEIC ACIDS RESEARCH

Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors

(2018) Silvana Konermann et al.   CELL

The Biology of CRISPR-Cas: Backward and Forward

(2018) Frank Hille et al.   CELL

Type III CRISPR-Cas System: Introduction And Its Application for Genetic Manipulations

(2018) Tao Liu et al.   CURRENT ISSUES IN MOLECULAR BIOLOGY

The ribonuclease activity of Csm6 is required for anti-plasmid immunity by Type III-A CRISPR-Cas systems

(2018) Kawanda Foster et al.   RNA Biology

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

Control of cyclic oligoadenylate synthesis in a type III CRISPR system

(2018) Christophe Rouillon et al.   eLife

Molecular mechanisms of CRISPR–Cas spacer acquisition

(2018) Jon McGinn et al.   NATURE REVIEWS MICROBIOLOGY

Lactococcus lactis type III-A CRISPR-Cas system cleaves bacteriophage RNA

(2018) Anne M. Millen et al.   RNA Biology

Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference

(2018) Lilan You et al.   CELL

CRISPR-Based Technologies: Impact of RNA-Targeting Systems

(2018) Michael P. Terns   MOLECULAR CELL

Type III-A CRISPR-Cas Csm Complexes: Assembly, Periodic RNA Cleavage, DNase Activity Regulation, and Autoimmunity

(2018) Ning Jia et al.   MOLECULAR CELL

Stress Reduction, Bacterial Style

(2017) Susan Gottesman   JOURNAL OF BACTERIOLOGY

Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28

(2017) Aaron A. Smargon et al.   MOLECULAR CELL

Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers

(2017) Ole Niewoehner et al.   NATURE

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

CRISPR-Cas: Adapting to change

(2017) Simon A. Jackson et al.   SCIENCE

A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems

(2017) Migle Kazlauskiene et al.   SCIENCE

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Type III CRISPR-Cas Immunity: Major Differences Brushed Aside

(2017) Gintautas Tamulaitis et al.   TRENDS IN MICROBIOLOGY

RNA and DNA Targeting by a Reconstituted Thermus thermophilus Type III-A CRISPR-Cas System

(2017) Tina Y. Liu et al.   PLoS One

Programmable type III-A CRISPR-Cas DNA targeting modules

(2017) H. Travis Ichikawa et al.   PLoS One

Degradation of Phage Transcripts by CRISPR-Associated RNases Enables Type III CRISPR-Cas Immunity

(2016) Wenyan Jiang et al.   CELL

RNA-activated DNA cleavage by the Type III-B CRISPR–Cas effector complex

(2016) Michael A. Estrella et al.   GENES & DEVELOPMENT

Bipartite recognition of target RNAs activates DNA cleavage by the Type III-B CRISPR–Cas system

(2016) Joshua R. Elmore et al.   GENES & DEVELOPMENT

Identification and functional study of type III-A CRISPR-Cas systems in clinical isolates of Staphylococcus aureus

(2016) Linyan Cao et al.   INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY

Spatiotemporal Control of Type III-A CRISPR-Cas Immunity: Coupling DNA Degradation with the Target RNA Recognition

(2016) Migle Kazlauskiene et al.   MOLECULAR CELL

A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction

(2016) Wenyuan Han et al.   NUCLEIC ACIDS RESEARCH

Multiple nucleic acid cleavage modes in divergent type III CRISPR systems

(2016) Jing Zhang et al.   NUCLEIC ACIDS RESEARCH

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity

(2015) Poulami Samai et al.   CELL

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Crystal Structure of the CRISPR-Cas RNA Silencing Cmr Complex Bound to a Target Analog

(2015) Takuo Osawa et al.   MOLECULAR CELL

Target RNA capture and cleavage by the Cmr type III-B CRISPR–Cas effector complex

(2014) Caryn R. Hale et al.   GENES & DEVELOPMENT

RNA Targeting by the Type III-A CRISPR-Cas Csm Complex of Thermus thermophilus

(2014) Raymond H.J. Staals et al.   MOLECULAR CELL

Programmable RNA Shredding by the Type III-A CRISPR-Cas System of Streptococcus thermophilus

(2014) Gintautas Tamulaitis et al.   MOLECULAR CELL

The three major types of CRISPR-Cas systems function independently in CRISPR RNA biogenesis inStreptococcus thermophilus

(2014) Jason Carte et al.   MOLECULAR MICROBIOLOGY

Conditional tolerance of temperate phages via transcription-dependent CRISPR-Cas targeting

(2014) Gregory W. Goldberg et al.   NATURE

Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials

(2014) David Bikard et al.   NATURE BIOTECHNOLOGY

CRISPR-mediated targeted mRNA degradation in the archaeon Sulfolobus solfataricus

(2014) Ziga Zebec et al.   NUCLEIC ACIDS RESEARCH

CRISPR-based technologies: prokaryotic defense weapons repurposed

(2014) Rebecca M. Terns et al.   TRENDS IN GENETICS

Molecular Mechanisms of RNA Interference

(2013) Ross C. Wilson et al.   Annual Review of Biophysics

DegP primarily functions as a protease for the biogenesis of β-barrel outer membrane proteins in the Gram-negative bacteriumEscherichia coli

(2013) Xi Ge et al.   FEBS Journal

Genetic Characterization of Antiplasmid Immunity through a Type III-A CRISPR-Cas System

(2013) A. Hatoum-Aslan et al.   JOURNAL OF BACTERIOLOGY

A Ruler Protein in a Complex for Antiviral Defense Determines the Length of Small Interfering CRISPR RNAs

(2013) Asma Hatoum-Aslan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure and Activity of the RNA-Targeting Type III-B CRISPR-Cas Complex of Thermus thermophilus

(2013) Raymond H.J. Staals et al.   MOLECULAR CELL

Structure of the CRISPR Interference Complex CSM Reveals Key Similarities with Cascade

(2013) Christophe Rouillon et al.   MOLECULAR CELL

Unexpectedly broad target recognition of the CRISPR-mediated virus defence system in the archaeon Sulfolobus solfataricus

(2013) Andrea Manica et al.   NUCLEIC ACIDS RESEARCH

Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs

(2012) Caryn R. Hale et al.   MOLECULAR CELL

Fast gapped-read alignment with Bowtie 2

(2012) Ben Langmead et al.   NATURE METHODS

RNA snap ™: a rapid, quantitative and inexpensive, method for isolating total RNA from bacteria

(2012) Mark B. Stead et al.   NUCLEIC ACIDS RESEARCH

BEDTools: a flexible suite of utilities for comparing genomic features

(2010) Aaron R. Quinlan et al.   BIOINFORMATICS

Structure of a bacterial ribonuclease P holoenzyme in complex with tRNA

(2010) Nicholas J. Reiter et al.   NATURE

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

Self versus non-self discrimination during CRISPR RNA-directed immunity

(2010) Luciano A. Marraffini et al.   NATURE

Of proteins and RNA: The RNase P/MRP family

(2010) O. Esakova et al.   RNA

The Sequence Alignment/Map format and SAMtools

(2009) H. Li et al.   BIOINFORMATICS

RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex

(2009) Caryn R. Hale et al.   CELL

RNAi mechanisms and applications

(2008) Daniel H. Kim et al.   BIOTECHNIQUES

Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotes

(2008) J. Carte et al.   GENES & DEVELOPMENT

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE