Novel CRISPR/Cas9-mediated knockout of LIG4 increases efficiency of site-specific integration in Chinese hamster ovary cell line

Improvements in Gene Editing Technology Boost Its Applications in Livestock

(2021) Iuri Viotti Perisse et al.   Frontiers in Genetics

Efficient TALEN-mediated gene knockin at the bovine Y chromosome and generation of a sex-reversal bovine

(2021) Ming Wang et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Targeted integration into pseudo attP sites of CHO cells using CRISPR/Cas9

(2021) Sana Pourtabatabaei et al.   JOURNAL OF BIOTECHNOLOGY

Decoupling Growth and Protein Production in CHO Cells: A Targeted Approach

(2021) James S. Donaldson et al.   Frontiers in Bioengineering and Biotechnology

Restoration of DNA repair mitigates genome instability and increases productivity of Chinese hamster ovary cells

(2021) Philipp N. Spahn et al.   BIOTECHNOLOGY AND BIOENGINEERING

Inhibition of DNA ligase IV enhances the CRISPR/Cas9-mediated knock-in efficiency in mouse brain neurons

(2020) Xueshan Cao et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Methods Favoring Homology-Directed Repair Choice in Response to CRISPR/Cas9 Induced-Double Strand Breaks

(2020) Han Yang et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

CRISPR-Cas9 system: A new-fangled dawn in gene editing

(2019) Darshana Gupta et al.   LIFE SCIENCES

The fickle CHO: a review of the causes, implications, and potential alleviation of the CHO cell line instability problem

(2019) Hussain Dahodwala et al.   CURRENT OPINION IN BIOTECHNOLOGY

Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35

(2018) Menglin Zhao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells

(2018) Xiaojie Ma et al.   Nature Communications

Nonhomologous DNA end-joining for repair of DNA double-strand breaks

(2017) Nicholas R. Pannunzio et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Dual loss of human POLQ and LIG4 abolishes random integration

(2017) Shinta Saito et al.   Nature Communications

Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA

(2017) Alex N. Zelensky et al.   Nature Communications

Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector

(2017) Ziqing Liu et al.   Scientific Reports

Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment

(2016) Jae Seong Lee et al.   BIOTECHNOLOGY AND BIOENGINEERING

RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency

(2016) Jun Song et al.   Nature Communications

Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases

(2016) Diane Yang et al.   Scientific Reports

The art of CHO cell engineering: A comprehensive retrospect and future perspectives

(2015) Simon Fischer et al.   BIOTECHNOLOGY ADVANCES

Inhibition of nonhomologous end joining to increase the specificity of CRISPR/Cas9 genome editing

(2015) Supriya V. Vartak et al.   FEBS Journal

Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway

(2015) Jae Seong Lee et al.   Scientific Reports

Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool

(2014) Carlotta Ronda et al.   BIOTECHNOLOGY AND BIOENGINEERING

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

BRCA1 Accelerates CtIP-Mediated DNA-End Resection

(2014) Andrés Cruz-García et al.   Cell Reports

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases

(2013) Kelly J. Beumer et al.   G3-Genes Genomes Genetics

Trex2 Enables Spontaneous Sister Chromatid Exchanges Without Facilitating DNA Double-Strand Break Repair

(2011) Lavinia C. Dumitrache et al.   GENETICS

The Mechanism of Double-Strand DNA Break Repair by the Nonhomologous DNA End-Joining Pathway

(2010) Michael R. Lieber   Annual Review of Biochemistry

Cell Engineering and Cultivation of Chinese Hamster Ovary (CHO) Cells

(2010) Takeshi Omasa et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Impact of non-homologous end-joining deficiency on random and targeted DNA integration: implications for gene targeting

(2008) Susumu Iiizumi et al.   NUCLEIC ACIDS RESEARCH