Engineered Cas9 extracellular vesicles as a novel gene editing tool

CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations

(2022) Ida Höijer et al.   Nature Communications

Long-term stable reduction of low-density lipoprotein in nonhuman primates following in vivo genome editing of PCSK9

(2021) Lili Wang et al.   MOLECULAR THERAPY

In vivo CRISPR base editing of PCSK9 durably lowers cholesterol in primates

(2021) Kiran Musunuru et al.   NATURE

In vivo adenine base editing of PCSK9 in macaques reduces LDL cholesterol levels

(2021) Tanja Rothgangl et al.   NATURE BIOTECHNOLOGY

An oral antisense oligonucleotide for PCSK9 inhibition

(2021) Peter Gennemark et al.   Science Translational Medicine

A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA

(2020) Olivier G. de Jong et al.   Nature Communications

Exosome-based delivery of super-repressor IκBα relieves sepsis-associated organ damage and mortality

(2020) Hojun Choi et al.   Science Advances

Advancements and Obstacles of CRISPR-Cas9 technology in Translational Research

(2019) Liting You et al.   Molecular Therapy-Methods & Clinical Development

Exosomal delivery of doxorubicin enables rapid cell entry and enhanced in vitro potency

(2019) Christina Schindler et al.   PLoS One

Friend or Foe? Evidence Indicates Endogenous Exosomes Can Deliver Functional gRNA and Cas9 Protein

(2019) Ran Chen et al.   Small

Endosomal escape enhancing compounds facilitate functional delivery of extracellular vesicle cargo

(2019) Nikki Heath et al.   Nanomedicine

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning

(2019) Daqi Wang et al.   Nature Communications

ARMMs as a versatile platform for intracellular delivery of macromolecules

(2018) Qiyu Wang et al.   Nature Communications

The CRISPR tool kit for genome editing and beyond

(2018) Mazhar Adli   Nature Communications

VSV-G-Enveloped Vesicles for Traceless Delivery of CRISPR-Cas9

(2018) Claudia Montagna et al.   Molecular Therapy-Nucleic Acids

Exosome-Liposome Hybrid Nanoparticles Deliver CRISPR/Cas9 System in MSCs

(2018) Yao Lin et al.   Advanced Science

Delivery approaches for CRISPR/Cas9 therapeutics in vivo: advances and challenges

(2018) D.C. Luther et al.   Expert Opinion on Drug Delivery

Cancer-derived exosomes as a delivery platform of CRISPR/Cas9 confer cancer cell tropism-dependent targeting

(2017) Seung Min Kim et al.   JOURNAL OF CONTROLLED RELEASE

Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer

(2017) Sushrut Kamerkar et al.   NATURE

Non-homologous DNA end joining and alternative pathways to double-strand break repair

(2017) Howard H. Y. Chang et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling

(2017) Youichi Uda et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The role of exosomes in cancer metastasis

(2017) Teresa Bernadette Steinbichler et al.   SEMINARS IN CANCER BIOLOGY

Exosomes, an Unmasked Culprit in Neurodegenerative Diseases

(2017) Federico N. Soria et al.   Frontiers in Neuroscience

Delivery of Therapeutic Proteins: Challenges and Strategies

(2016) Kanwal Rehman et al.   CURRENT DRUG TARGETS

Extracellular vesicles in cardiovascular disease: are they Jedi or Sith?

(2016) Xabier Osteikoetxea et al.   JOURNAL OF PHYSIOLOGY-LONDON

Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein–protein interaction module

(2016) Nambin Yim et al.   Nature Communications

A role for peptides in overcoming endosomal entrapment in siRNA delivery — A focus on melittin

(2015) Kirk K. Hou et al.   BIOTECHNOLOGY ADVANCES

Sequence determinants of improved CRISPR sgRNA design

(2015) Han Xu et al.   GENOME RESEARCH

Microvesicle- and exosome-mediated drug delivery enhances the cytotoxicity of Paclitaxel in autologous prostate cancer cells

(2015) Heikki Saari et al.   JOURNAL OF CONTROLLED RELEASE

Cell-derived vesicles for drug therapy and diagnostics: Opportunities and challenges

(2015) Gregor Fuhrmann et al.   Nano Today

Differential detergent sensitivity of extracellular vesicle subpopulations

(2015) Xabier Osteikoetxea et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Improved Characterization of EV Preparations Based on Protein to Lipid Ratio and Lipid Properties

(2015) Xabier Osteikoetxea et al.   PLoS One

Visualization and tracking of tumour extracellular vesicle delivery and RNA translation using multiplexed reporters

(2015) Charles P. Lai et al.   Nature Communications

Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins

(2015) Fuun Kawano et al.   Nature Communications

The Legacy of Nat Sternberg: The Genesis of Cre-loxTechnology

(2015) Michael Yarmolinsky et al.   Annual Review of Virology

Easy quantitative assessment of genome editing by sequence trace decomposition

(2014) Eva K. Brinkman et al.   NUCLEIC ACIDS RESEARCH

Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations

(2014) Yao-Cheng Lin et al.   Nature Communications

Bioinspired Exosome-Mimetic Nanovesicles for Targeted Delivery of Chemotherapeutics to Malignant Tumors

(2013) Su Chul Jang et al.   ACS Nano

Synthesis of phycocyanobilin in mammalian cells

(2013) Konrad Müller et al.   CHEMICAL COMMUNICATIONS

Protein translocation as a tool: The current rapamycin story

(2012) Mateusz Putyrski et al.   FEBS LETTERS

Genetically Engineered Microvesicles Carrying Suicide mRNA/Protein Inhibit Schwannoma Tumor Growth

(2012) Arda Mizrak et al.   MOLECULAR THERAPY

FLASH: fast length adjustment of short reads to improve genome assemblies

(2011) T. Magoc et al.   BIOINFORMATICS

Protein Targeting to Exosomes/Microvesicles by Plasma Membrane Anchors

(2011) Beiyi Shen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Rapid blue-light–mediated induction of protein interactions in living cells

(2010) Matthew J Kennedy et al.   NATURE METHODS

Fast and accurate short read alignment with Burrows-Wheeler transform

(2009) H. Li et al.   BIOINFORMATICS

Spatiotemporal control of cell signalling using a light-switchable protein interaction

(2009) Anselm Levskaya et al.   NATURE