Preparation of a Six-Enzyme Multilayer Combi-Biocatalyst: Reuse of the Most Stable Enzymes after Inactivation of the Least Stable One

Coimmobilization of lipases exhibiting three very different stability ranges. Reuse of the active enzymes and selective discarding of the inactivated ones

(2022) Diego Carballares et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Biolubricant Production from Several Oleaginous Feedstocks Using Lipases as Catalysts: Current Scenario and Future Perspectives

(2021) Iara C. A. Bolina et al.   BioEnergy Research

Cold Active Lipases: Biocatalytic Tools for Greener Technology

(2021) Nutan Mhetras et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

A phenylalanine dynamic switch controls the interfacial activation of Rhizopus chinensis lipase

(2021) Shang Wang et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Advantages of Supports Activated with Divinyl Sulfone in Enzyme Coimmobilization: Possibility of Multipoint Covalent Immobilization of the Most Stable Enzyme and Immobilization via Ion Exchange of the Least Stable Enzyme

(2021) Roberto Morellon-Sterling et al.   ACS Sustainable Chemistry & Engineering

Co-Immobilization of Rhizopus oryzae and Candida rugosa Lipases onto mMWCNTs@4-arm-PEG-NH2—A Novel Magnetic Nanotube–Polyethylene Glycol Amine Composite—And Its Applications for Biodiesel Production

(2021) Saadiah A. Abdulmalek et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Influence of phosphate anions on the stability of immobilized enzymes. Effect of enzyme nature, immobilization protocol and inactivation conditions

(2020) Jakub F. Kornecki et al.   PROCESS BIOCHEMISTRY

Ultrasound-assisted hydrolysis of lard for free fatty acids catalyzed by combined two lipases in aqueous medium

(2020) Jinjin Huang et al.   Bioengineered

Effects of Enzyme Loading and Immobilization Conditions on the Catalytic Features of Lipase From Pseudomonas fluorescens Immobilized on Octyl-Agarose Beads

(2020) Sara Arana-Peña et al.   Frontiers in Bioengineering and Biotechnology

Biotechnological relevance of the lipase A from Candida antarctica

(2020) Rodolpho R.C. Monteiro et al.   CATALYSIS TODAY

One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates

(2020) Sara Arana-Peña et al.   Catalysts

Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?

(2020) Sara Arana-Peña et al.   BIOTECHNOLOGY ADVANCES

Multi‐enzyme Cascade Reactions in Metal‐Organic Frameworks

(2020) Jieying Liang et al.   CHEMICAL RECORD

Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production

(2020) Sara Arana-Peña et al.   ENZYME AND MICROBIAL TECHNOLOGY

Immobilized Biocatalysts of Eversa® Transform 2.0 and Lipase from Thermomyces Lanuginosus: Comparison of Some Properties and Performance in Biodiesel Production

(2020) Javier A. Martínez-Sanchez et al.   Catalysts

Immunomodulatory activities of body wall fatty acids extracted from Halocynthia aurantium on RAW264.7 cells

(2020) Chaiwat Monmai et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Liquid lipase preparations designed for industrial production of biodiesel. Is it really an optimal solution?

(2020) Rodolpho R.C. Monteiro et al.   RENEWABLE ENERGY

Multi-Combilipases: Co-Immobilizing Lipases with Very Different Stabilities Combining Immobilization via Interfacial Activation and Ion Exchange. The Reuse of the Most Stable Co-Immobilized Enzymes after Inactivation of the Least Stable Ones

(2020) Sara Arana-Peña et al.   Catalysts

Improvement of biodiesel production from palm oil by co-immobilization of Thermomyces lanuginosa lipase and Candida antarctica lipase B: Optimization using response surface methodology

(2020) Mansour Shahedi et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

(2019) Alejandra Sánchez-Bayo et al.   Catalysts

Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions

(2019) Rafael C. Rodrigues et al.   BIOTECHNOLOGY ADVANCES

Tailoring Multipurpose Biocatalysts via Protein Engineering Approaches: A Review

(2019) Muhammad Bilal et al.   CATALYSIS LETTERS

Reuse of Lipase from Pseudomonas fluorescens via Its Step-by-Step Coimmobilization on Glyoxyl-Octyl Agarose Beads with Least Stable Lipases

(2019) Rios et al.   Catalysts

New applications of glyoxyl-octyl agarose in lipases co-immobilization: Strategies to reuse the most stable lipase

(2019) Sara Arana-Peña et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Novel Combi-lipase Systems for Fatty Acid Ethyl Esters Production

(2019) Esteban C. Toro et al.   Catalysts

Increasing the Enzyme Loading Capacity of Porous Supports by a Layer-by-Layer Immobilization Strategy Using PEI as Glue

(2019) Nathalia S. Rios et al.   Catalysts

Lecitase ultra: A phospholipase with great potential in biocatalysis

(2019) Jose J. Virgen-Ortíz et al.   Molecular Catalysis

Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering

(2019) Sara Arana-Peña et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Improving the Yields and Reaction Rate in the Ethanolysis of Soybean Oil by Using Mixtures of Lipase CLEAs

(2019) Margarita Díaz Ramos et al.   MOLECULES

Parameters necessary to define an immobilized enzyme preparation

(2019) Joseph Boudrant et al.   PROCESS BIOCHEMISTRY

Co-immobilization of Rhizomucor miehei lipase and Candida antarctica lipase B and optimization of biocatalytic biodiesel production from palm oil using response surface methodology

(2019) Mansour Shahedi et al.   RENEWABLE ENERGY

Transesterification of Waste Frying Oil and Soybean Oil by Combi-lipases Under Ultrasound-Assisted Reactions

(2018) Jakeline Kathiele Poppe et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Enzymatic synthesis of ethyl esters from waste oil using mixtures of lipases in a plug-flow packed-bed continuous reactor

(2018) Jakeline Kathiele Poppe et al.   BIOTECHNOLOGY PROGRESS

One-step Synthesis of α-Keto Acids from Racemic Amino Acids by A Versatile Immobilized Multienzyme Cell-free System

(2018) Alejandro H. Orrego et al.   ChemCatChem

How lipase technology contributes to evolution of biodiesel production using multiple feedstocks

(2018) Shinji Hama et al.   CURRENT OPINION IN BIOTECHNOLOGY

Stabilization of Immobilized Lipases by Intense Intramolecular Cross-Linking of Their Surfaces by Using Aldehyde-Dextran Polymers

(2018) Alejandro Orrego et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Elucidation of lid open and orientation of lipase activated in interfacial activation by amphiphilic environment

(2018) Cheng Cheng et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Optimization of a dual-functional biocatalytic system for continuous hydrolysis of lactose in milk

(2018) Heng Li et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Improved stability of immobilized lipases via modification with polyethylenimine and glutaraldehyde

(2017) Hadjer Zaak et al.   ENZYME AND MICROBIAL TECHNOLOGY

Relevance of substrates and products on the desorption of lipases physically adsorbed on hydrophobic supports

(2017) Jose J. Virgen-Ortíz et al.   ENZYME AND MICROBIAL TECHNOLOGY

Prevention of Bacterial Contamination of a Silica Matrix Containing Entrapped β-Galactosidase through the Action of Covalently Bound Lysozymes

(2017) Heng Li et al.   MOLECULES

Coimmobilization of enzymes in bilayers using pei as a glue to reuse the most stable enzyme: Preventing pei release during inactivated enzyme desorption

(2017) Hadjer Zaak et al.   PROCESS BIOCHEMISTRY

Conversion of Chlamydomonas sp. JSC4 lipids to biodiesel using Fusarium heterosporum lipase-expressing A sp ergillus oryzae whole-cell as biocatalyst

(2017) Jerome Amoah et al.   Algal Research-Biomass Biofuels and Bioproducts

Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts

(2017) Jose J. Virgen-Ortíz et al.   Journal of Materials Chemistry B

Optimisation of combi-lipases from Aspergillus niger for the synergistic and efficient hydrolysis of soybean oil

(2016) Hanzhen Qiao et al.   ANIMAL SCIENCE JOURNAL

Biodiesel production using immobilized lipase: feasibility and challenges

(2016) Revathy Sankaran et al.   Biofuels Bioproducts & Biorefining-Biofpr

Lipase cocktail for efficient conversion of oils containing phospholipids to biodiesel

(2016) Jerome Amoah et al.   BIORESOURCE TECHNOLOGY

Preparation of biodiesel from nonedible oils using a mixture of used lipases

(2016) Sanjib Kumar Karmee   Energy Sources Part A-Recovery Utilization and Environmental Effects

Application of Deep Eutectic Solvents in Promiscuous Lipase-Catalysed Aldol Reactions

(2016) Daniel González-Martínez et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Effect of Molecular Structure on the Relative Hydrogen Peroxide Scavenging Ability of Some α-Keto Carboxylic Acids

(2016) Antonio Lopalco et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Immobilized multienzymatic systems for catalysis of cascade reactions

(2016) Qingzhi Ji et al.   PROCESS BIOCHEMISTRY

Insight into the synthesis of isosorbide diester plasticizer using immobilized lipases

(2016) Caixia Cui et al.   RSC Advances

Development of simple protocols to solve the problems of enzyme coimmobilization. Application to coimmobilize a lipase and a β-galactosidase

(2016) Sara Peirce et al.   RSC Advances

A roadmap for biocatalysis - functional and spatial orchestration of enzyme cascades

(2016) Claudia Schmidt-Dannert et al.   Microbial Biotechnology

Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases

(2015) Jakeline Kathiele Poppe et al.   APPLIED CATALYSIS A-GENERAL

Immobilization of lipases on hydrophobic supports involves the open form of the enzyme

(2015) Evelin A. Manoel et al.   ENZYME AND MICROBIAL TECHNOLOGY

Tuning the catalytic properties of lipases immobilized on divinylsulfone activated agarose by altering its nanoenvironment

(2015) Jose C.S. dos Santos et al.   ENZYME AND MICROBIAL TECHNOLOGY

Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications

(2015) Grazia Borrelli et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Lipase catalyzed interesterification of Amazonian patauá oil and palm stearin for preparation of specific-structured oils

(2015) Paula Speranza et al.   JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE

Combining phospholipases and a liquid lipase for one-step biodiesel production using crude oils

(2014) Silvia Cesarini et al.   Biotechnology for Biofuels

Improving the catalytic properties of immobilized Lecitase via physical coating with ionic polymers

(2014) Jose C.S. dos Santos et al.   ENZYME AND MICROBIAL TECHNOLOGY

Dynamic heterogeneity controls diffusion and viscosity near biological interfaces

(2014) Sander Pronk et al.   Nature Communications

Combi-lipase for heterogeneous substrates: a new approach for hydrolysis of soybean oil using mixtures of biocatalysts

(2014) Joana S. Alves et al.   RSC Advances

Materials-based strategies for multi-enzyme immobilization and co-localization: A review

(2013) Feng Jia et al.   BIOTECHNOLOGY AND BIOENGINEERING

Enzymatic Synthesis of Extra Virgin Olive Oil Based Infant Formula Fat Analogues Containing ARA and DHA: One-Stage and Two-Stage Syntheses

(2013) Garima Pande et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production

(2013) Jong Ho Lee et al.   KOREAN JOURNAL OF CHEMICAL ENGINEERING

Kinetic modeling of biodiesel production by mixed immobilized and co-immobilized lipase systems under two pressure conditions

(2013) Jong Ho Lee et al.   KOREAN JOURNAL OF CHEMICAL ENGINEERING

The combi-CLEA approach: enzymatic cascade synthesis of enantiomerically pure (S)-mandelic acid

(2013) Andrzej Chmura et al.   TETRAHEDRON-ASYMMETRY

Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization

(2013) Oveimar Barbosa et al.   RSC Advances

Rational Co-Immobilization of Bi-Enzyme Cascades on Porous Supports and their Applications in Bio-Redox Reactions with In Situ Recycling of Soluble Cofactors

(2012) Javier Rocha-Martín et al.   ChemCatChem

Lipase applications in oil hydrolysis with a case study on castor oil: a review

(2012) Debajyoti Goswami et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Hydrogen Peroxide in Biocatalysis. A Dangerous Liaison

(2012) Karel Hernandez et al.   CURRENT ORGANIC CHEMISTRY

Functional Properties and Bioactivities of Pine Nut (Pinus gerardiana) Protein Isolates and Its Enzymatic Hydrolysates

(2012) Luyun Cai et al.   Food and Bioprocess Technology

Lipase promiscuity and its biochemical applications

(2012) Manali Kapoor et al.   PROCESS BIOCHEMISTRY

Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

(2011) Cristina Garcia-Galan et al.   ADVANCED SYNTHESIS & CATALYSIS

Multi-Enzymatic Cascade Reactions: Overview and Perspectives

(2011) Emanuele Ricca et al.   ADVANCED SYNTHESIS & CATALYSIS

Hydrolysis of triacetin catalyzed by immobilized lipases: Effect of the immobilization protocol and experimental conditions on diacetin yield

(2011) Karel Hernandez et al.   ENZYME AND MICROBIAL TECHNOLOGY

Biodiesel production from crude canola oil by two-step enzymatic processes

(2011) Myung Gwi Jang et al.   RENEWABLE ENERGY

Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process

(2010) Jong Ho Lee et al.   BIORESOURCE TECHNOLOGY

Intraparticle concentration gradients for substrate and acidic product in immobilized cephalosporin C amidase and their dependencies on carrier characteristics and reaction parameters

(2010) Caterina Boniello et al.   BIOTECHNOLOGY AND BIOENGINEERING

Biodiesel preparation catalyzed by compound-lipase in co-solvent

(2010) Qin Li et al.   FUEL PROCESSING TECHNOLOGY

Mixed lipases for efficient enzymatic synthesis of biodiesel from used palm oil and ethanol in a solvent-free system

(2010) Ketsara Tongboriboon et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Lipase from Rhizomucor miehei as an industrial biocatalyst in chemical process

(2010) Rafael C. Rodrigues et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Lipase from Rhizomucor miehei as a biocatalyst in fats and oils modification

(2010) Rafael C. Rodrigues et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Combination of two lipases more efficiently catalyzes methanolysis of soybean oil for biodiesel production in aqueous medium

(2010) Feifei Guan et al.   PROCESS BIOCHEMISTRY

Effects of the combined use of Thermomyces lanuginosus and Rhizomucor miehei lipases for the transesterification and hydrolysis of soybean oil

(2010) Rafael C. Rodrigues et al.   PROCESS BIOCHEMISTRY

Synthesis of Aliphatic (S)-α-Hydroxycarboxylic Amides using a One-Pot Bienzymatic Cascade of Immobilised Oxynitrilase and Nitrile Hydratase

(2009) Sander van Pelt et al.   ADVANCED SYNTHESIS & CATALYSIS

Transesterification for biodiesel production catalyzed by combined lipases: Optimization and kinetics

(2009) Yun Liu et al.   AICHE JOURNAL

Development of Batch and Continuous Processes on Biodiesel Production in a Packed-Bed Reactor by a Mixture of Immobilized Candida rugosa and Rhizopus oryzae Lipases

(2009) Jong Ho Lee et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Multi-enzymatic synthesis

(2009) Fernando Lopez-Gallego et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Lipase from Thermomyces lanuginosus: Uses and prospects as an industrial biocatalyst

(2009) Roberto Fernandez-Lafuente   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Lipases at interfaces: A review

(2008) P. Reis et al.   ADVANCES IN COLLOID AND INTERFACE SCIENCE

Optimization of Lipase-Catalyzed Transesterification of Lard for Biodiesel Production Using Response Surface Methodology

(2008) Ying Huang et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY