Spacer2PAM: A computational framework to guide experimental determination of functional CRISPR-Cas system PAM sequences

Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale

(2022) Fungmin Eric Liew et al.   NATURE BIOTECHNOLOGY

Repurposing the Endogenous Type I-E CRISPR/Cas System for Gene Repression in Gluconobacter oxydans WSH-003

(2021) Zhijie Qin et al.   ACS Synthetic Biology

Exploiting heterologous and endogenous CRISPR‐Cas systems for genome editing in the probiotic Clostridium butyricum

(2021) Xiuqing Zhou et al.   BIOTECHNOLOGY AND BIOENGINEERING

Stepping on the Gas to a Circular Economy: Accelerating Development of Carbon-Negative Chemical Production from Gas Fermentation

(2021) Nick Fackler et al.   Annual Review of Chemical and Biomolecular Engineering

In Vivo Targeting of Clostridioides difficile Using Phage-Delivered CRISPR-Cas3 Antimicrobials

(2020) Kurt Selle et al.   mBio

In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design

(2020) Ashty S. Karim et al.   Nature Chemical Biology

Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities

(2020) Jeanne E. van Dongen et al.   BIOSENSORS & BIOELECTRONICS

Prophages are associated with extensive CRISPR–Cas auto-immunity

(2020) Franklin L Nobrega et al.   NUCLEIC ACIDS RESEARCH

Applications of CRISPR Technologies Across the Food Supply Chain

(2019) Katelyn Brandt et al.   Annual Review of Food Science and Technology

Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum

(2019) Florence J. Annan et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A Novel Conjugal Donor Strain for Improved DNA transfer into Clostridium spp.

(2019) Craig Woods et al.   ANAEROBE

Genome editing using the endogenous type I CRISPR-Cas system in Lactobacillus crispatus

(2019) Claudio Hidalgo-Cantabrana et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Characterization and repurposing of the endogenous Type I-F CRISPR–Cas system of Zymomonas mobilis for genome engineering

(2019) Yanli Zheng et al.   NUCLEIC ACIDS RESEARCH

Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production

(2018) Jie Zhang et al.   METABOLIC ENGINEERING

A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs

(2018) Colin S. Maxwell et al.   METHODS

CRISPRCasFinder, an update of CRISRFinder, includes a portable version, enhanced performance and integrates search for Cas proteins

(2018) David Couvin et al.   NUCLEIC ACIDS RESEARCH

High-Level dCas9 Expression Induces Abnormal Cell Morphology in Escherichia coli

(2018) Suhyung Cho et al.   ACS Synthetic Biology

In Silico Processing of the Complete CRISPR-Cas Spacer Space for Identification of PAM Sequences

(2018) Brian J. Mendoza et al.   Biotechnology Journal

Deciphering, Communicating, and Engineering the CRISPR PAM

(2017) Ryan T. Leenay et al.   JOURNAL OF MOLECULAR BIOLOGY

Metabolic engineering of Clostridium autoethanogenum for selective alcohol production

(2017) Fungmin Liew et al.   METABOLIC ENGINEERING

CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum

(2017) Jae Sung Cho et al.   METABOLIC ENGINEERING

In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni

(2017) Eunji Kim et al.   Nature Communications

A thermostable Cas9 with increased lifetime in human plasma

(2017) Lucas B. Harrington et al.   Nature Communications

Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform

(2017) Jeremy M. Rock et al.   Nature Microbiology

Genome editing of Clostridium autoethanogenum using CRISPR/Cas9

(2016) Shilpa Nagaraju et al.   Biotechnology for Biofuels

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

(2016) Ryan T. Leenay et al.   MOLECULAR CELL

Applications of CRISPR technologies in research and beyond

(2016) Rodolphe Barrangou et al.   NATURE BIOTECHNOLOGY

Programmable control of bacterial gene expression with the combined CRISPR and antisense RNA system

(2016) Young Je Lee et al.   NUCLEIC ACIDS RESEARCH

PHASTER: a better, faster version of the PHAST phage search tool

(2016) David Arndt et al.   NUCLEIC ACIDS RESEARCH

Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium

(2016) Michael E. Pyne et al.   Scientific Reports

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR-cas Subtype I-Fb in Acinetobacter baumannii: Evolution and Utilization for Strain Subtyping

(2015) Nabil Karah et al.   PLoS One

CRISPR-Cas Systems: Prokaryotes Upgrade to Adaptive Immunity

(2014) Rodolphe Barrangou et al.   MOLECULAR CELL

Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials

(2014) David Bikard et al.   NATURE BIOTECHNOLOGY

Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems

(2014) A. A. Gomaa et al.   mBio

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

(2013) Kevin M Esvelt et al.   NATURE METHODS

CRISPRTarget

(2013) Ambarish Biswas et al.   RNA Biology

A Genetic System for Clostridium ljungdahlii: a Chassis for Autotrophic Production of Biocommodities and a Model Homoacetogen

(2012) Ching Leang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The CRISPR/Cas Adaptive Immune System of Pseudomonas aeruginosa Mediates Resistance to Naturally Occurring and Engineered Phages

(2012) K. C. Cady et al.   JOURNAL OF BACTERIOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE