Dynamic modulation of enzyme activity by synthetic CRISPR–Cas6 endonucleases

Structure of the native pyruvate dehydrogenase complex reveals the mechanism of substrate insertion

(2021) Jana Škerlová et al.   Nature Communications

Flux controlling technology for central carbon metabolism for efficient microbial bio-production

(2020) Yoshihiro Toya et al.   CURRENT OPINION IN BIOTECHNOLOGY

Controlling metabolic flux by toehold-mediated strand displacement

(2020) Rebecca P Chen et al.   CURRENT OPINION IN BIOTECHNOLOGY

Light-based control of metabolic flux through assembly of synthetic organelles

(2019) Evan M. Zhao et al.   Nature Chemical Biology

Artificial Multienzyme Scaffolds: Pursuing in Vitro Substrate Channeling with an Overview of Current Progress

(2019) Gregory A. Ellis et al.   ACS Catalysis

Model-Assisted Fine-Tuning of Central Carbon Metabolism in Yeast through dCas9-Based Regulation

(2019) Raphael Ferreira et al.   ACS Synthetic Biology

Dynamic protein assembly by programmable DNA strand displacement

(2018) Rebecca P. Chen et al.   Nature Chemistry

Riboregulated toehold-gated gRNA for programmable CRISPR–Cas9 function

(2018) Ka-Hei Siu et al.   Nature Chemical Biology

Direct cell-to-cell exchange of matter in a synthetic Clostridium syntrophy enables CO2 fixation, superior metabolite yields, and an expanded metabolic space

(2018) Kamil Charubin et al.   METABOLIC ENGINEERING

Spatial organization of heterologous metabolic system in vivo based on TALE

(2016) Lv-yun Zhu et al.   Scientific Reports

Engineering metabolism through dynamic control

(2015) Naveen Venayak et al.   CURRENT OPINION IN BIOTECHNOLOGY

Plasticity of specialized metabolism as mediated by dynamic metabolons

(2015) Tomas Laursen et al.   TRENDS IN PLANT SCIENCE

Toehold Switches: De-Novo-Designed Regulators of Gene Expression

(2014) Alexander A. Green et al.   CELL

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Unravelling the structural and mechanistic basis of CRISPR–Cas systems

(2014) John van der Oost et al.   NATURE REVIEWS MICROBIOLOGY

In vivoco-localization of enzymes on RNA scaffolds increases metabolic production in a geometrically dependent manner

(2014) Gairik Sachdeva et al.   NUCLEIC ACIDS RESEARCH

Cas6 specificity and CRISPR RNA loading in a complex CRISPR-Cas system

(2014) Richard D. Sokolowski et al.   NUCLEIC ACIDS RESEARCH

In vitro assembly and activity of an archaeal CRISPR-Cas type I-A Cascade interference complex

(2014) André Plagens et al.   NUCLEIC ACIDS RESEARCH

Improving fatty acids production by engineering dynamic pathway regulation and metabolic control

(2014) P. Xu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biomolecular scaffolds for enhanced signaling and catalytic efficiency

(2013) Rebecca Chen et al.   CURRENT OPINION IN BIOTECHNOLOGY

Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases

(2013) O. Niewoehner et al.   NUCLEIC ACIDS RESEARCH

On the biophysics and kinetics of toehold-mediated DNA strand displacement

(2013) Niranjan Srinivas et al.   NUCLEIC ACIDS RESEARCH

Quantitative Design of Regulatory Elements Based on High-Precision Strength Prediction Using Artificial Neural Network

(2013) Hailin Meng et al.   PLoS One

Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate

(2012) Mary P. Hall et al.   ACS Chemical Biology

Combinatorial Design of a Highly Efficient Xylose-Utilizing Pathway in Saccharomyces cerevisiae for the Production of Cellulosic Biofuels

(2012) Byoungjin Kim et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production

(2012) Mitchell Tai et al.   METABOLIC ENGINEERING

Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids

(2012) Fuzhong Zhang et al.   NATURE BIOTECHNOLOGY

Natural strategies for the spatial optimization of metabolism in synthetic biology

(2012) Christina M Agapakis et al.   Nature Chemical Biology

Production of amorphadiene in yeast, and its conversion to dihydroartemisinic acid, precursor to the antimalarial agent artemisinin

(2012) P. J. Westfall et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ATP drives direct photosynthetic production of 1-butanol in cyanobacteria

(2012) E. I. Lan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mechanism of substrate selection by a highly specific CRISPR endoribonuclease

(2012) S. H. Sternberg et al.   RNA

Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae

(2011) Nancy A. Da Silva et al.   FEMS YEAST RESEARCH

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Structural basis for CRISPR RNA-guided DNA recognition by Cascade

(2011) Matthijs M Jore et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Organization of Intracellular Reactions with Rationally Designed RNA Assemblies

(2011) C. J. Delebecque et al.   SCIENCE

NUPACK: Analysis and design of nucleic acid systems

(2010) Joseph N. Zadeh et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Dynamic Metabolons

(2010) B. L. Moller   SCIENCE

Synthetic protein scaffolds provide modular control over metabolic flux

(2009) John E Dueber et al.   NATURE BIOTECHNOLOGY

Engineering the spatial organization of metabolic enzymes: mimicking nature's synergy

(2008) Robert J Conrado et al.   CURRENT OPINION IN BIOTECHNOLOGY

Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotes

(2008) J. Carte et al.   GENES & DEVELOPMENT