4.7 Article

Structure characteristics and functionality of water-soluble fraction from high-intensity ultrasound treated pea protein isolate

Journal

FOOD HYDROCOLLOIDS
Volume 125, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2021.107409

Keywords

Plant protein; Pea protein isolate; Ultrasound power; Soluble aggregates; Emulsion; Foaming

Funding

  1. China Scholarship Council (CSC)

Ask authors/readers for more resources

The study investigates the impact of ultrasonic treatment on water-soluble pea protein, showing changes in secondary and tertiary structure, increased protein content and solubility, adverse effects on emulsification properties, and improved foaming stability.
High-intensity ultrasound (HIUS) has been widely utilized as an emerging technique to modify the functionality of proteins. However, those findings exclusively pertained to the whole protein after the treatment of HIUS. Such protein consists of not only water-soluble proteins but other insoluble constituents, which cannot reflect the role of HIUS on the protein functionality. The objective of this study is to understand the influences of ultrasonic treatment (20 kHz) at varying power (200, 300 or 500 W) and time (5, 10 or 20 min) exclusively on structural and functional properties of water-soluble fraction (WPPI) harvested from HIUS treated pea protein isolate (PPI). Relevant parameters of ultrasound including ultrasonic intensity and ultrasonic density were determined. No significant changes on the primary structure of WPPI were observed from electrophoresis and size exclusion chromatography analysis. Fourier-transform infrared spectroscopy analysis as well as intrinsic and extrinsic fluorescence spectroscopy showed that HIUS was able to change the secondary and tertiary structure of WPPI. HIUS treatment drastically improved protein content and absolute solubility of pea protein. The underlying mechanism lies in the roles of HIUS to change the secondary and tertiary structure and to increase the magnitude of surface charge of WPPI, as well as to facilitate the coexistence of both smaller particle size pea protein and soluble aggregates. HIUS exerted an adverse effect on emulsification properties but improved foaming stability. The results of this study provide a theoretical basis to unravel the essential role of HIUS on modifying the structure of plant protein and the corresponding functionality.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Review Food Science & Technology

Modification of pulse proteins for improved functionality and flavor profile: A comprehensive review

Fengchao Zha, Jiajia Rao, Bingcan Chen

Summary: Consumers' preference for healthier eating habits has increased the demand for nutrient-dense plant-based foods, with pulse proteins being considered as beneficial ingredients for promoting health in new food products. However, challenges such as poor functionality and unfavorable taste have hindered the use of pulse proteins in foods. Protein structure modification has been shown to be an effective way to improve the functionality and flavor of pulse proteins, with techniques such as chemical modifications, enzymatic modifications, and molecular breeding being explored. Understanding the relationship between protein structure and functionality is crucial for tailoring proteins for specific functional purposes and expanding the availability of pulse proteins. Selective protein modification is a valuable tool for creating novel protein structures with desired functional attributes and flavor profiles. Innovative structure modifications focused on the molecular basis of protein design are essential for enhancing the functionality of pulse proteins.

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY (2021)

Article Chemistry, Applied

Toward a comprehensive understanding of ultracentrifugal milling on the physicochemical properties and aromatic profile of yellow pea flour

Zixuan Gu, Haiyang Jiang, Fengchao Zha, Frank Manthey, Jiajia Rao, Bingcan Chen

Summary: This study investigated the effect of ultracentrifugal mill configurations on the properties of yellow pea flour, finding that different screen aperture sizes significantly impacted pasting and thermal properties of the flour. Additionally, 2-ethyl-1-hexanol could potentially be used as an aroma marker to distinguish between excessive milling or inappropriate mill configurations.

FOOD CHEMISTRY (2021)

Article Chemistry, Applied

Plant proteins from green pea and chickpea: Extraction, fractionation, structural characterization and functional properties

Liuyi Chang, Yang Lan, Nonoy Bandillo, Jae-Bom Ohm, Bingcan Chen, Jiajia Rao

Summary: The study investigated the structural and functional attributes, as well as the aroma profile of major fractions of pulse storage proteins isolated from green pea and chickpea. Different protein compositions had significant impacts on the structural and functional properties of proteins. Chickpea protein fractions contained higher typical beany aromatic compounds than green pea protein fractions.

FOOD HYDROCOLLOIDS (2022)

Article Biochemistry & Molecular Biology

Statistical evaluation to validate matrix-matched calibration for standardized beany odor compound quantitation in yellow pea flour using HS-SPME-GC-MS

Zixuan Gu, Xiao Chen, Jiajia Rao, Bingcan Chen

Summary: This study established a standardized external calibration quantitation method using accelerated dichloromethane extraction on YP flour and starch. Weighted least squares regression was introduced to build calibration curves, confirming the feasibility of deodorized starch as an alternative for quantifying beany odor compounds.

FOOD & FUNCTION (2022)

Article Chemistry, Applied

Unraveling the mechanism by which high intensity ultrasound improves the solubility of commercial pea protein isolates

Kun Gao, Jiajia Rao, Bingcan Chen

Summary: In this study, the solubility of commercial pea protein isolate (PPI) was significantly improved through high intensity ultrasound treatment. The research provides unprecedented insights into the underlying mechanisms of how ultrasound improves protein solubility.

FOOD HYDROCOLLOIDS (2022)

Article Chemistry, Applied

Reverse micelles extraction of hemp protein isolate: Impact of defatting process on protein structure, functionality, and aromatic profile

Baochen Fang, Zixuan Gu, Jae-Bom Ohm, Bingcan Chen, Jiajia Rao

Summary: This study investigated the influence of the defatting process on the extraction performance and properties of hemp protein isolate (HPI) obtained via reverse micelles (RMs) extraction. The results showed that defatting is not necessary for protein extraction and HPIs obtained from non-defatted hemp flour and defatted hemp flour exhibited similar composition, functionalities, and protein subunits. However, HPI from non-defatted hemp flour exhibited stronger gel properties and higher content of aromatic compounds.

FOOD HYDROCOLLOIDS (2023)

Article Chemistry, Applied

Structure modification, functionality and interfacial properties of kidney bean (Phaseolus vulgaris L.) protein concentrate as affected by post-extraction treatments

Uyory Choe, Liuyi Chang, Jae-Bom Ohm, Bingcan Chen, Jiajia Rao

Summary: In this study, the structural, functional, and interfacial properties of kidney bean protein concentrate (KPC) treated with different methods were comprehensively evaluated. The treatments led to changes in surface hydrophobicity, tryptophan fluorescence, sulfhydryl groups, and disulfide bonds of KPC. In terms of functional properties, the solubility, emulsifying activity index (EAI), and emulsifying stability index (ESI) were improved in certain treatments. The interfacial properties indicated the relationship between EAI and diffusion rate, and the formation of a viscoelastic film influenced ESI.

FOOD HYDROCOLLOIDS (2022)

Article Food Science & Technology

Fractionation, Structural Characteristics, Functionality, Aromatic Profile, and In Vitro Digestibility of Lentil (Lens culinaris) Proteins

Liuyi Chang, Zixuan Gu, Nonoy Bandillo, Bingcan Chen, Jiajia Rao

Summary: Consumers' positive attitudes toward plant-based diets have led to the exploration of functional plant proteins. This study investigated the properties of protein fractions from underexploited lentil grain, including their structure, function, nutrition, aroma, and in vitro digestion. The albumin (LA) and vicilin (LV) fractions exhibited unique structural properties, high solubility, and foaming capacity, while the globulin (LG) fraction had better emulsifying properties. LA had a distinct aromatic profile and lower hydrolysis degree after in vitro digestion due to the presence of antinutritional factors.

ACS FOOD SCIENCE & TECHNOLOGY (2023)

Article Chemistry, Applied

Interfacial, and emulsifying properties nexus of green pea protein fractions: Impact of pH and salt

Liuyi Chang, Yang Lan, Bingcan Chen, Jiajia Rao

Summary: Pea protein isolate has gained attention for its high nutritional value and emulsifying properties, but its application in food emulsions is limited due to insufficient knowledge. This study investigated the effects of pH and NaCl concentrations on the physicochemical and interfacial properties of different pea protein fractions and their relationship with emulsifying properties. Results showed that higher pH and NaCl concentrations improved emulsifying ability, while lower pH had the opposite effect. Additionally, NaCl weakened emulsions and albumin had the highest emulsifying ability but was most affected by NaCl in terms of stability.

FOOD HYDROCOLLOIDS (2023)

Article Chemistry, Applied

Synergistic effect of pH-shift and controlled heating on improving foaming properties of pea vicilin and its adsorption behavior at the air-water interface

Liuyi Chang, Bingcan Chen, Jiajia Rao

Summary: This study comprehensively evaluated the effects of pH-shift, controlled heating, high intensity ultrasound, and their combinations on the structural, foaming, and surface behavior of modified pea vicilin protein fraction. The combination of pH-shift and controlled heating showed the greatest improvement in foaming capacity, while ultrasound treatment had the least effect. The improved foaming capacity of the modified protein was attributed to the formation of soluble aggregates, transition of secondary structure, and increased surface hydrophobicity.

FOOD HYDROCOLLOIDS (2023)

Article Biochemistry & Molecular Biology

Nutraceutical potential of industrial hemp (Cannabis sativa L.) extracts: physicochemical stability and bioaccessibility of cannabidiol (CBD) nanoemulsions

Huijuan Zheng, Bingcan Chen, Jiajia Rao

Summary: A food-grade CBD nanoemulsion system was developed using MCT, CO, or HSO as the carrier oil, showing good physicochemical stability and bioaccessibility. Light exposure and acidity were found to be two important factors affecting the chemical stability of CBD. CBD in the nanoemulsion showed improved bioaccessibility compared to bulk oil forms.

FOOD & FUNCTION (2022)

Article Food Science & Technology

Comparison of the Proximate Compositions, Nutritional Minerals, Pasting Properties, and Aroma Differences of Flours from Selected Yellow Pea Cultivars Grown across the Northern Great Plains

Haiyang Jiang, Zixuan Gu, Frank Manthey, Bingcan Chen, Jiajia Rao

Summary: The study explored the differences in functionality and aromatic compounds of yellow pea flours from seven cultivars, highlighting the essential impact of cultivar variation on the physicochemical properties and aromatic profile of the flour.

ACS FOOD SCIENCE & TECHNOLOGY (2021)

Article Biochemistry & Molecular Biology

The viability of complex coacervate encapsulated probiotics during simulated sequential gastrointestinal digestion affected by wall materials and drying methods

Xiaoxi Qi, Yang Lan, Jae-Bom Ohm, Bingcan Chen, Jiajia Rao

Summary: The study found that protein type and mixing ratio can affect complex coacervate formation and properties of LGG microcapsules, while the drying method has a significant impact on the viability and microstructure of encapsulated LGG. Spray-dried microcapsules showed better performance in terms of cell loss and maintained higher viable cell counts after digestion compared to freeze-dried samples.

FOOD & FUNCTION (2021)

Article Biochemistry & Molecular Biology

Physicochemical property changes and aroma differences of fermented yellow pea flours: role of Lactobacilli and fermentation time

Chun Li, Xiaoqian Chen, Zhao Jin, Zixuan Gu, Jiajia Rao, Bingcan Chen

Summary: The physicochemical properties and aroma of yellow pea flours fermented by different lactic acid bacteria strains were evaluated in this study. The fermentation time and specific strains significantly influenced the cell population, chemical composition, amino acid content, and aroma compounds of the fermented flours. Lactobacilli fermentation improved the pasting properties and aromatic profile of the flours, with L. acidophilus producing three specific aromatic compounds that positively contributed to the aroma.

FOOD & FUNCTION (2021)

Article Chemistry, Applied

Ethanol-mediated electrospinning of casein-only bead-free nanofibers

Deepika Sharma, Gregory R. Ziegler, Federico M. Harte

Summary: Fabrication of bead-free nanofibers from pure casein is challenging due to its self-aggregation tendency. In this study, the influence of pH, ethanol content, ionic environment, and casein concentration on the solubility and solution characteristics of casein-based spinning dopes was analyzed. The addition of tetrasodium pyrophosphate (TSPP) reduced bead defects and improved the properties of the nanofibers. The viscosity of the solution depended on the casein concentration, and bead-free nanofibers were obtained at a concentration higher than the entanglement concentration (C-e).

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

Fate of pulse globulin proteins molecular Structure and composition on high moisture extrusion

Smriti Shrestha, Leonie Van't Hag, Victoria Haritos, Sushil Dhital

Summary: This study investigated the pasting properties, structural formation, and molecular changes of mungbean, green lentil, and yellow pea proteins during high moisture extrusion (HME). The results showed that protein composition and conformation played important roles in the structural development and molecular changes during HME.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

Utilization of self-assembled soy protein nanoparticles as carriers for natural pigments: Examining non-interaction mechanisms and stability

Shi Liu, Na Sun, Kunyu Ren, Xubin Tan, Lanxin Li, Zhe Wang, Shicheng Dai, Xiaohong Tong, Huan Wang, Lianzhou Jiang

Summary: In this study, self-assembled soy protein nanoparticles (SPN) were successfully prepared and used for encapsulation of lutein, 8-carotene, and curcumin. The encapsulated natural pigments showed improved stability, especially curcumin. This study provides a new approach for extending the use of lipophilic natural edible pigments in food products.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

Effect of pH on the formation mechanisms, emulsifying properties and curcumin encapsulation of oat protein isolate-high methoxy pectin complexes

Jianming Wang, Ziyun Liu, Kaiwen Zheng, Zhe Yuan, Chen Yang

Summary: By combining with high methoxyl pectin (HMP), the emulsifying properties of oat protein isolate (OPI) were improved. The OPI-HMP complex formed soluble complexes under specific pH conditions, exhibiting excellent emulsifying activity and stability, making it suitable as a delivery system for lipid soluble bioactive compounds in food and biomedical applications.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

A new strategy for maintaining the thermal stability of phycocyanin under acidic conditions: pH-induced whey protein isolate-phycocyanin coprecipitation forms composite with chitosan

Zihao Yin, Junzhe Zou, Mengwei Wang, Ruonan Huang, Yuemiao Qian, Mingyong Zeng, Fangwei Li

Summary: Phycocyanin, a protein prone to degradation under acidic conditions and unstable at high temperatures, has limited application in food. This study successfully combined phycocyanin with whey protein isolate using protein coprecipitation and chitosan coating to form composite particles, which maintained the color and colloidal stability of phycocyanin. The composite particles showed minimal color changes and retained high pigment retention rates after heating. Furthermore, the composite particles exhibited excellent antioxidant capacity. This breakthrough expands the application of phycocyanin in acidic beverages and other food forms.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

Enhancing the bioavailability of quercetin via the construction of carboxymethylated curdlan/quercetin nanocomplex

Yuting Su, Qian Zhou, Hui Xu, Manting Huang, Siqian Li, Jiayi He, Ka-Wing Cheng, Mingfu Wang

Summary: By developing the CM-Cur/QT complex, the solubility and stability of quercetin were improved, leading to enhanced bioavailability. The complex showed increased bio-accessibility and antioxidant efficacy during in vitro digestion, and facilitated the internalization of quercetin in macrophages, exerting a potential synergistic anti-inflammatory effect.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

Natural pH and temperature-sensitive hydrogel-emulsion carriers for co-delivery of hydrophobic and hydrophilic bioactive ingredients in the gastrointestinal tract

Sheliang Zhao, David Julian Mcclements, Xuebo Liu, Fuguo Liu

Summary: The low oral bioavailability of some bioactive ingredients is a challenge in the supplement and functional food areas. This study designs natural carriers that can simultaneously deliver both hydrophobic and hydrophilic bioactive ingredients, thereby increasing their ability to exert synergistic effects.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

Engineering biodegradable controlled gelatin-zein bilayer film with improved mechanical strength and flexibility

Md Easdani, Shabbir Ahammed, Md Nazmus Saqib, Fei Liu, Fang Zhong

Summary: Compiling hydrophobic and hydrophilic proteins to form a mechanically reliable bilayer film with improved water barrier properties and hydrophobicity is challenging. By introducing cross-linkers, the film exhibits compatible and flexible surfaces, enhanced color and transparency, and controlled water vapor permeability. The bilayer film shows potential for improving value-added functional properties.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

Construction and properties of curdlan gum/gellan gum binary composite gel system

Tian-Qi Zhou, Xue-Chen Wang, Ling-Yi Gao, Jia-Nan Yan, Hai-Tao Wu

Summary: This study investigated the synergetic gelation of curdlan gum (CG) and gellan gum (GG) at different ratios. The optimal gelation was achieved at a mass ratio of 1:1 and a total concentration of 0.6%. The gel properties were influenced by the CG/GG ratio, with the strongest gel observed at a ratio of 5:5. The improved gel properties were attributed to hydrogen bonds between CG and GG. The CG/GG binary composite gel exhibited increased water holding capacity as the CG/GG ratio decreased. Furthermore, the microstructure of the CG/GG gel was denser and more homogeneous, supporting its stronger gel rigidity and water holding capacity.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

Porous gelatin microspheres for high loading and improved stability of anthocyanins: Serving as antioxidant factors and food freshness indicators

Jialin Sun, Zihao Wei, Changhu Xue

Summary: In this study, porous gelatin microspheres (PGMs) with surface and internal porous structure were developed for the controlled release and rapid functional response of anthocyanins (ANCs). The loading of ACNs was achieved through electrostatic interaction, resulting in a high loading capacity. ACN@PGMs exhibited long-term controlled release, improved storage stability, and ammonia responsiveness, making them suitable for applications as antioxidant factors and food freshness indicators in the food industry.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

A novel baking additive: Preparation, characterization, and application of chitosan hydrochloride/carboxymethyl starch sodium nano-gel for wheat bread

Xinlai Dou, Yanling Hao, Ying Sun, Pin Yang, Linlin Liu, Yinyuan He, Yanguo Shi, Chunhua Yang, Fenglian Chen

Summary: In this study, chitosan hydrochloride/carboxymethyl starch sodium (CHC-CMS-Na) nanogels were prepared as a baking additive and showed potential in improving bread quality.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

Synergism between yellow mustard gum and κ-carrageenan studied by structural and rheological methods

Xinya Wang, H. Douglas Goff, Steve W. Cui

Summary: This study thoroughly investigated the synergistic gelation between yellow mustard gum (YMG) and kappa-carrageenan using multiple techniques. The results showed that the interaction between YMG and kappa-carrageenan was mainly polymer associations, and YMG affected the gel formation process of kappa-carrageenan.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

Effect of enzymatic dephosphorylation on caprine casein micelle structure

Jielong Zhang, Dasong Liu, Xiumei Tao, Jun Tang, Xiaoyu Peng, Thom Huppertz, Xiaoming Liu, Peng Zhou

Summary: The effect of enzymatic dephosphorylation on the structure of casein micelles in caprine micellar casein concentrate was studied. The results showed that with increasing dephosphorylation, the molar mass decreased, the radii decreased, the hydration increased, and the internal protein inhomogeneity disappeared.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

Highly-branched cyclic dextrin for improvement in mechanical properties and freeze-thaw stability of x-carrageenan gels

Wentao Ma, Chao Yuan, Bo Cui, Tian Gao, Li Guo, Bin Yu, Meng Zhao, Feixue Zou

Summary: This study investigated the influence of highly-branched cyclic dextrin (HBCD) on the physiochemical properties of x-carrageenan (KC) gels. The addition of HBCD significantly enhanced the storage modulus and freeze-thaw stability of KC gels. HBCD promoted the distribution of KC chains and aggregation of KC helixes, resulting in a compact network structure and reduced water loss. Furthermore, HBCD strengthened the thermal reversibility of KC gels through enhanced hydrogen-bonding interactions.

FOOD HYDROCOLLOIDS (2024)

Article Chemistry, Applied

Enhanced solubility, thermal stability and antioxidant activity of resveratrol by complexation with ovalbumin amyloid-like fibrils: Effect of pH

Ya-Ru Wang, Qin Yang, Yi-Xuan Jiang, Han-Qing Chen

Summary: This study successfully improved the aqueous solubility, thermal stability, and antioxidant activity of RES by using ovalbumin fibrils as nanocarriers. The pH value was found to have an impact on the formation and properties of the complex, with the best interaction observed at pH 2.0.

FOOD HYDROCOLLOIDS (2024)