Advances in amplification-free detection of nucleic acid: CRISPR/Cas system as a powerful tool

CRISPR-based DNA and RNA detection with liquid-liquid phase separation

(2021) Willem Kasper Spoelstra et al.   BIOPHYSICAL JOURNAL

Cas12a-based electrochemiluminescence biosensor for target amplification-free DNA detection

(2021) Peng-Fei Liu et al.   BIOSENSORS & BIOELECTRONICS

CRISPR-powered electrochemical microfluidic multiplexed biosensor for target amplification-free miRNA diagnostics

(2021) Richard Bruch et al.   BIOSENSORS & BIOELECTRONICS

Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor

(2021) Sarah Balderston et al.   Nature Biomedical Engineering

Amplification-free RNA detection with CRISPR–Cas13

(2021) Hajime Shinoda et al.   Communications Biology

Droplet Cas12a Assay Enables DNA Quantification from Unamplified Samples at the Single-Molecule Level

(2021) Huahua Yue et al.   NANO LETTERS

Universal and Naked-Eye Gene Detection Platform Based on the Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a/13a System

(2020) Chaoqun Yuan et al.   ANALYTICAL CHEMISTRY

CRISPR/Cas12a-Mediated Interfacial Cleaving of Hairpin DNA Reporter for Electrochemical Nucleic Acid Sensing

(2020) Decai Zhang et al.   ACS Sensors

Surpassing the detection limit and accuracy of the electrochemical DNA sensor through the application of CRISPR Cas systems

(2020) Wei Xu et al.   BIOSENSORS & BIOELECTRONICS

High-throughput and all-solution phase African Swine Fever Virus (ASFV) detection using CRISPR-Cas12a and fluorescence based point-of-care system

(2020) Qian He et al.   BIOSENSORS & BIOELECTRONICS

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage

(2020) Tautvydas Karvelis et al.   NUCLEIC ACIDS RESEARCH

Sequence-Specific Recognition of HIV-1 DNA with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN)

(2020) Reza Nouri et al.   ACS Sensors

CRISPR-dCas9 powered impedimetric biosensor for label-free detection of circulating tumor DNAs

(2020) Zihni Onur Uygun et al.   ANALYTICA CHIMICA ACTA

Magnetic Bead-Quantum Dot (MB-Qdot) Clustered Regularly Interspaced Short Palindromic Repeat Assay for Simple Viral DNA Detection

(2020) Mengdi Bao et al.   ACS Applied Materials & Interfaces

Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities

(2020) Jeanne E. van Dongen et al.   BIOSENSORS & BIOELECTRONICS

A lateral flow strip combined with Cas9 nickase-triggered amplification reaction for dual food-borne pathogen detection

(2020) Luying Wang et al.   BIOSENSORS & BIOELECTRONICS

Clustered Regularly Interspaced Short Palindromic Repeats-Mediated Surface-Enhanced Raman Scattering Assay for Multidrug-Resistant Bacteria

(2020) Hongki Kim et al.   ACS Nano

An Ultralocalized Cas13a Assay Enables Universal and Nucleic Acid Amplification-Free Single-Molecule RNA Diagnostics

(2020) Tian Tian et al.   ACS Nano

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

(2020) Parinaz Fozouni et al.   CELL

CRISPR-Cas12a-Based Nucleic Acid Amplification-Free DNA Biosensor via Au Nanoparticle-Assisted Metal-Enhanced Fluorescence and Colorimetric Analysis

(2020) Jin-Ha Choi et al.   NANO LETTERS

Fabrication of ultrasensitive electrochemical biosensor for dengue fever viral RNA Based on CRISPR/Cpf1 reaction

(2020) Yeonju Lee et al.   SENSORS AND ACTUATORS B-CHEMICAL

Light-up RNA aptamer signaling-CRISPR-Cas13a-based mix-and-read assays for profiling viable pathogenic bacteria

(2020) Ting Zhang et al.   BIOSENSORS & BIOELECTRONICS

Ultrasensitive electrochemical assay for microRNA-21 based on CRISPR/Cas13a-assisted catalytic hairpin assembly

(2020) Ying Cui et al.   TALANTA

Engineering of CRISPR-Cas12b for human genome editing

(2019) Jonathan Strecker et al.   Nature Communications

Rapid and Fully Microfluidic Ebola Virus Detection with CRISPR-Cas13a

(2019) Peiwu Qin et al.   ACS Sensors

Attomolar Label-Free Detection of DNA Hybridization with Electrolyte-Gated Graphene Field-Effect Transistors

(2019) Rui Campos et al.   ACS Sensors

Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor

(2019) Reza Hajian et al.   Nature Biomedical Engineering

CRISPR/Cas Powered Multiplexed Biosensing

(2019) Richard Bruch et al.   TRENDS IN BIOTECHNOLOGY

Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d

(2019) Bo Zhang et al.   Nature Communications

Multiplexed DNA identification using site specific dCas9 barcodes and nanopore sensing

(2019) Nicole E. Weckman et al.   ACS Sensors

Unamplified gene sensing via Cas9 on graphene

(2019) Richard Bruch et al.   Nature Biomedical Engineering

CRISPR-Cas12a Coupled with Platinum Nanoreporter for Visual Quantification of SNVs on a Volumetric Bar-Chart Chip

(2019) Ning Shao et al.   ANALYTICAL CHEMISTRY

Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor

(2019) Yifan Dai et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Advances in Detecting and Reducing Off-target Effects Generated by CRISPR-mediated Genome Editing

(2019) Jin-Jing Li et al.   Journal of Genetics and Genomics

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA

(2018) Shi-Yuan Li et al.   CELL RESEARCH

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Detection of CRISPR-dCas9 on DNA with Solid-State Nanopores

(2018) Wayne Yang et al.   NANO LETTERS

PAM identification by CRISPR-Cas effector complexes: diversified mechanisms and structures

(2018) Daniel Gleditzsch et al.   RNA Biology

Engineered CRISPR-Cas9 nuclease with expanded targeting space

(2018) Hiroshi Nishimasu et al.   SCIENCE

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

Nicking enzyme-assisted amplification (NEAA) technology and its applications: A review

(2018) Cheng Qian et al.   ANALYTICA CHIMICA ACTA

Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a

(2017) Daan C. Swarts et al.   MOLECULAR CELL

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA

(2016) Takashi Yamano et al.   CELL

Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage

(2016) F. Jiang et al.   SCIENCE

Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems

(2016) Prarthana Mohanraju et al.   SCIENCE

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Conformational control of DNA target cleavage by CRISPR–Cas9

(2015) Samuel H. Sternberg et al.   NATURE

Multicolor CRISPR labeling of chromosomal loci in human cells

(2015) Hanhui Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrasensitive Label-Free Detection of PNA–DNA Hybridization by Reduced Graphene Oxide Field-Effect Transistor Biosensor

(2014) Bingjie Cai et al.   ACS Nano

Nanopore Sequencing: From Imagination to Reality

(2014) H. Bayley   CLINICAL CHEMISTRY

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

(2013) Baohui Chen et al.   CELL

MicroRNA: Function, Detection, and Bioanalysis

(2013) Haifeng Dong et al.   CHEMICAL REVIEWS

Absolute quantification by droplet digital PCR versus analog real-time PCR

(2013) Christopher M Hindson et al.   NATURE METHODS

The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems

(2013) Krzysztof Chylinski et al.   RNA Biology

Engineering the pH-Responsive Catalytic Behavior of AuNPs by DNA

(2013) Pengfei Zhan et al.   Small

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Rapid Detection and Limitations of Molecular Techniques

(2012) John J. Maurer   Annual Review of Food Science and Technology

Sequence- and Structure-Specific RNA Processing by a CRISPR Endonuclease

(2010) Rachel E. Haurwitz et al.   SCIENCE

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE