Microfluidic fabrication of lipid nanoparticles for the delivery of nucleic acids

CAR T cells produced in vivo to treat cardiac injury

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A noninflammatory mRNA vaccine for treatment of experimental autoimmune encephalomyelitis

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Membrane-destabilizing ionizable phospholipids for organ-selective mRNA delivery and CRISPR–Cas gene editing

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Robotics, microfluidics, nanotechnology and AI in the synthesis and evaluation of liposomes and polymeric drug delivery systems

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Lipid Nanoparticles as Delivery Systems for RNA-Based Vaccines

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In vivo CRISPR base editing of PCSK9 durably lowers cholesterol in primates

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In vivo adenine base editing of PCSK9 in macaques reduces LDL cholesterol levels

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Insight Into the Prospects for RNAi Therapy of Cancer

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Microfluidic formulation of nanoparticles for biomedical applications

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Scalable mRNA and siRNA Lipid Nanoparticle Production Using a Parallelized Microfluidic Device

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Lipid nanoparticles for mRNA delivery

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The Effect of Size and Charge of Lipid Nanoparticles Prepared by Microfluidic Mixing on Their Lymph Node Transitivity and Distribution

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Ionizable Lipid Nanoparticle-Mediated mRNA Delivery for Human CAR T Cell Engineering

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The promise and challenge of therapeutic genome editing

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Driving CARs to last

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Highly efficient and tumor-selective nanoparticles for dual-targeted immunogene therapy against cancer

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MTL-CEBPA, a small activating RNA therapeutic up-regulating C/EBP-α, in patients with advanced liver cancer: a first-in-human, multi-centre, open-label, phase I trial

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Using microfluidics for scalable manufacturing of nanomedicines from bench to GMP: A case study using protein-loaded liposomes

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Antisolvent precipitation of lipid nanoparticles in microfluidic systems – A comparative study

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A shared tumor-antigen RNA-lipoplex vaccine with/without anti-PD1 in patients with checkpoint-inhibition experienced melanoma.

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Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR–Cas gene editing

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Lipid nanoparticle technology for therapeutic gene regulation in the liver

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Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning

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SARS-CoV-2 mRNA vaccine design enabled by prototype pathogen preparedness

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Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

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88MO T-cell responses induced by an individualized neoantigen specific immune therapy in post (neo)adjuvant patients with triple negative breast cancer

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Advances in Lipid Nanoparticles for mRNA-Based Cancer Immunotherapy

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Recent Advances in Microfluidics for the Preparation of Drug and Gene Delivery Systems

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The Effect of Physical Cues of Biomaterial Scaffolds on Stem Cell Behavior

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Manufacturing Considerations for the Development of Lipid Nanoparticles Using Microfluidics

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Lipid nanoparticles loaded with ribonucleoprotein–oligonucleotide complexes synthesized using a microfluidic device exhibit robust genome editing and hepatitis B virus inhibition

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The current state and future directions of RNAi-based therapeutics

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Highly efficient therapeutic gene editing of human hematopoietic stem cells

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Recent Developments in mRNA-Based Protein Supplementation Therapy to Target Lung Diseases

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How mRNA therapeutics are entering the monoclonal antibody field

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Messenger RNA therapy for rare genetic metabolic diseases

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Evaluating and Enhancing Target Specificity of Gene-Editing Nucleases and Deaminases

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Adenine base editing in an adult mouse model of tyrosinaemia

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Nanoparticles: Oral Delivery for Protein and Peptide Drugs

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Nanoparticles for Nucleic Acid Delivery: Applications in Cancer Immunotherapy

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Lipid Nanoparticles for Delivery of Therapeutic RNA Oligonucleotides

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Sterically stabilized liposomes production using staggered herringbone micromixer: Effect of lipid composition and PEG-lipid content

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Fluid mechanics and process design of high-pressure antisolvent precipitation of fenofibrate nanoparticles using a customized microsystem

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Clinical-Scale Production of CAR-T Cells for the Treatment of Melanoma Patients by mRNA Transfection of a CSPG4-Specific CAR under Full GMP Compliance

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Enlisting the mRNA Vaccine Platform to Combat Parasitic Infections

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A review on novel methodologies for drug nanoparticle preparation: Microfluidic approach

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The Onpattro story and the clinical translation of nanomedicines containing nucleic acid-based drugs

(2019) Akin Akinc et al.   Nature Nanotechnology

Advances in microfluidics for lipid nanoparticles and extracellular vesicles and applications in drug delivery systems

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An inert 3D emulsification device for individual precipitation and concentration of amorphous drug nanoparticles

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Factors relating to the biodistribution & clearance of nanoparticles & their effects on in vivo application

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mRNA vaccines — a new era in vaccinology

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Neutralization of the Plasmodium-encoded MIF ortholog confers protective immunity against malaria infection

(2018) Alvaro Baeza Garcia et al.   Nature Communications

Lipid Nanoparticles Enabling Gene Therapies: From Concepts to Clinical Utility

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A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing

(2018) Jonathan D. Finn et al.   Cell Reports

Transfection reagent Lipofectamine triggers type I interferon signaling activation in macrophages

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Gene editing restores dystrophin expression in a canine model of Duchenne muscular dystrophy

(2018) Leonela Amoasii et al.   SCIENCE

CRISPR-Cas guides the future of genetic engineering

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mRNA-Based Protein Replacement Therapy for the Heart

(2018) Ajit Magadum et al.   MOLECULAR THERAPY

Cell specific delivery of modified mRNA expressing therapeutic proteins to leukocytes

(2018) Nuphar Veiga et al.   Nature Communications

The Human Gene Mutation Database: towards a comprehensive repository of inherited mutation data for medical research, genetic diagnosis and next-generation sequencing studies

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Optically monitored segmented flow for controlled ultra-fast mixing and nanoparticle precipitation

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Preclinical and Clinical Demonstration of Immunogenicity by mRNA Vaccines against H10N8 and H7N9 Influenza Viruses

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Evolution and clinical translation of drug delivery nanomaterials

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Zika virus protection by a single low-dose nucleoside-modified mRNA vaccination

(2017) Norbert Pardi et al.   NATURE

Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing

(2017) Hao Yin et al.   NATURE BIOTECHNOLOGY

Topical tissue nano-transfection mediates non-viral stroma reprogramming and rescue

(2017) Daniel Gallego-Perez et al.   Nature Nanotechnology

N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density

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The Race of 10 Synthetic RNAi-Based Drugs to the Pharmaceutical Market

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Triboelectric Nanogenerator Accelerates Highly Efficient Nonviral Direct Conversion and In Vivo Reprogramming of Fibroblasts to Functional Neuronal Cells

(2016) Yoonhee Jin et al.   ADVANCED MATERIALS

Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system

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Non-viral delivery of genome-editing nucleases for gene therapy

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Elucidation of the physicochemical properties and potency of siRNA-loaded small-sized lipid nanoparticles for siRNA delivery

(2016) Yusuke Sato et al.   JOURNAL OF CONTROLLED RELEASE

Influence of particle size on the in vivo potency of lipid nanoparticle formulations of siRNA

(2016) Sam Chen et al.   JOURNAL OF CONTROLLED RELEASE

The effect of nanoparticle size on in vivo pharmacokinetics and cellular interaction

(2016) Nazanin Hoshyar et al.   Nanomedicine

Systemic RNA delivery to dendritic cells exploits antiviral defence for cancer immunotherapy

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Sustained antigen availability during germinal center initiation enhances antibody responses to vaccination

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A Glu-urea-Lys Ligand-conjugated Lipid Nanoparticle/siRNA System Inhibits Androgen Receptor Expression In Vivo

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51. Results of a Phase I Trial of SGT-53: A Systemically Administered, Tumor-Targeting Immunoliposome Nanocomplex Incorporating a Plasmid Encoding wtp53

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Liposome production by microfluidics: potential and limiting factors

(2016) Dario Carugo et al.   Scientific Reports

siRNA Lipid Nanoparticle Potently Silences Clusterin and Delays Progression When Combined with Androgen Receptor Cotargeting in Enzalutamide-Resistant Prostate Cancer

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Expression kinetics of nucleoside-modified mRNA delivered in lipid nanoparticles to mice by various routes

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Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

(2015) Ayal Hendel et al.   NATURE BIOTECHNOLOGY

ClinVar: public archive of interpretations of clinically relevant variants

(2015) Melissa J. Landrum et al.   NUCLEIC ACIDS RESEARCH

A strategy for synthesis of lipid nanoparticles using microfluidic devices with a mixer structure

(2015) Masatoshi Maeki et al.   RSC Advances

First-in-Human Phase I Study of the Liposomal RNA Interference Therapeutic Atu027 in Patients With Advanced Solid Tumors

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Lipid nanoparticles: Drug localization is substance-specific and achievable load depends on the size and physical state of the particles

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Intranasal mRNA nanoparticle vaccination induces prophylactic and therapeutic anti-tumor immunity

(2014) Kyle K. L. Phua et al.   Scientific Reports

Solid lipid nanoparticles

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Liposomal drug delivery systems: From concept to clinical applications

(2012) Theresa M. Allen et al.   ADVANCED DRUG DELIVERY REVIEWS

Designing Chemically Modified Oligonucleotides for Targeted Gene Silencing

(2012) Glen F. Deleavey et al.   CHEMISTRY & BIOLOGY

Solid lipid nanoparticles: Continuous and potential large-scale nanoprecipitation production in static mixers

(2012) Yuancai Dong et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Formulation of Poorly Water-Soluble Compound Loaded Solid Lipid Nanoparticles in a Microchannel System Fabricated by Mechanical Microcutting Method: Puerarin as a Model Drug

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Rapid Discovery of Potent siRNA-Containing Lipid Nanoparticles Enabled by Controlled Microfluidic Formulation

(2012) Delai Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Bottom-Up Design and Synthesis of Limit Size Lipid Nanoparticle Systems with Aqueous and Triglyceride Cores Using Millisecond Microfluidic Mixing

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Novel 3D manufacturing method combining microelectrial discharge machining and electrochemical polishing

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A mini review of nanosuspensions development

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Accumulation of sub-100 nm polymeric micelles in poorly permeable tumours depends on size

(2011) H. Cabral et al.   Nature Nanotechnology

Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation

(2010) Bart R. Anderson et al.   NUCLEIC ACIDS RESEARCH

Flow regimes for adiabatic gas–liquid flow in microchannels

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Knocking down barriers: advances in siRNA delivery

(2009) Kathryn A. Whitehead et al.   NATURE REVIEWS DRUG DISCOVERY

Formation of solid lipid nanoparticles in a microchannel system with a cross-shaped junction

(2008) Songhong Zhang et al.   CHEMICAL ENGINEERING SCIENCE

Chemical Modification of siRNAs for In Vivo Use

(2008) Mark A. Behlke   OLIGONUCLEOTIDES

Nanoparticles for drug delivery: The need for precision in reporting particle size parameters

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