Insights into HIV uncoating from single-particle imaging techniques

Cone-shaped HIV-1 capsids are transported through intact nuclear pores

(2021) Vojtech Zila et al.   CELL

HIV-1 uncoating occurs via a series of rapid biomechanical changes in the core related to individual stages of reverse transcription

(2021) Sanela Rankovic et al.   JOURNAL OF VIROLOGY

HIV-1 cores retain their integrity until minutes before uncoating in the nucleus

(2021) Chenglei Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A New Generation of Functional Tagged Proteins for HIV Fluorescence Imaging

(2021) João I. Mamede et al.   Viruses-Basel

Imaging Viral Infection by Fluorescence Microscopy: Focus on HIV-1 Early Stage

(2021) Soumajit Mukherjee et al.   Viruses-Basel

The HIV-1 Capsid: From Structural Component to Key Factor for Host Nuclear Invasion

(2021) Viviana Scoca et al.   Viruses-Basel

A lysine ring in HIV capsid pores coordinates IP6 to drive mature capsid assembly

(2021) Nadine Renner et al.   PLoS Pathogens

Sec24C is an HIV-1 host dependency factor crucial for virus replication

(2021) Stephanie V. Rebensburg et al.   Nature Microbiology

A stable immature lattice packages IP6 for HIV capsid maturation

(2021) Donna L. Mallery et al.   Science Advances

HIV-1 uncoating by release of viral cDNA from capsid-like structures in the nucleus of infected cells

(2021) Thorsten G Müller et al.   eLife

CALM supports clathrin-coated vesicle completion upon membrane tension increase

(2021) Nathan M. Willy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

HIV-1 CA Inhibitors Are Antagonized by Inositol Phosphate Stabilization of the Viral Capsid in Cells

(2021) Gregory A. Sowd et al.   JOURNAL OF VIROLOGY

Capsid labelled HIV to investigate the role of capsid during nuclear import and integration

(2020) Irena Zurnic Bönisch et al.   JOURNAL OF VIROLOGY

HIV-1 uncoats in the nucleus near sites of integration

(2020) Ryan C. Burdick et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Characterization of HIV-1 uncoating in human microglial cell lines

(2020) Zachary Ingram et al.   Virology Journal

Remodeling of the Core Leads HIV-1 Preintegration Complex into the Nucleus of Human Lymphocytes

(2020) Guillermo Blanco-Rodriguez et al.   JOURNAL OF VIROLOGY

Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus

(2020) Adarsh Dharan et al.   Nature Microbiology

In vitro quantification of the effects of IP6 and other small polyanions on immature HIV-1 particle assembly and core stability

(2020) Alžběta Dostálková et al.   JOURNAL OF VIROLOGY

HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains

(2020) Ashwanth C. Francis et al.   Nature Communications

Disrupting HIV ‐1 capsid formation causes cGAS sensing of viral DNA

(2020) Rebecca P Sumner et al.   EMBO JOURNAL

Reconstitution and visualization of HIV-1 capsid-dependent replication and integration in vitro

(2020) Devin E. Christensen et al.   SCIENCE

Clustering and reverse transcription of HIV‐1 genomes in nuclear niches of macrophages

(2020) Elena Rensen et al.   EMBO JOURNAL

The Host Cell Metabolite Inositol Hexakisphosphate Promotes Efficient Endogenous HIV-1 Reverse Transcription by Stabilizing the Viral Capsid

(2020) Jordan Jennings et al.   mBio

Permeability of the HIV-1 capsid to metabolites modulates viral DNA synthesis

(2020) Chaoyi Xu et al.   PLOS BIOLOGY

Analysis of CA Content and CPSF6 Dependence of Early HIV-1 Replication Complexes in SupT1-R5 Cells

(2019) Vojtech Zila et al.   mBio

A simple, high-throughput stabilization assay to test HIV-1 uncoating inhibitors

(2019) Alžběta Dostálková et al.   Scientific Reports

Cellular IP6 Levels Limit HIV Production while Viruses that Cannot Efficiently Package IP6 Are Attenuated for Infection and Replication

(2019) Donna L. Mallery et al.   Cell Reports

Single HIV-1 Imaging Reveals Progression of Infection through CA-Dependent Steps of Docking at the Nuclear Pore, Uncoating, and Nuclear Transport

(2018) Ashwanth C. Francis et al.   Cell Host & Microbe

HIV-1 activation of innate immunity depends strongly on the intracellular level of TREX1 and sensing of incomplete reverse transcription products

(2018) Swati Kumar et al.   JOURNAL OF VIROLOGY

Truncated CPSF6 Forms Higher-Order Complexes That Bind and Disrupt HIV-1 Capsid

(2018) Jiying Ning et al.   JOURNAL OF VIROLOGY

Kinetics of HIV-1 capsid uncoating revealed by single-molecule analysis

(2018) Chantal L Márquez et al.   eLife

IP6 is an HIV pocket factor that prevents capsid collapse and promotes DNA synthesis

(2018) Donna L Mallery et al.   eLife

Inositol phosphates are assembly co-factors for HIV-1

(2018) Robert A. Dick et al.   NATURE

Reverse Transcription Mechanically Initiates HIV-1 Capsid Disassembly

(2017) Sanela Rankovic et al.   JOURNAL OF VIROLOGY

Early cytoplasmic uncoating is associated with infectivity of HIV-1

(2017) João I. Mamede et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy

(2016) François Aguet et al.   MOLECULAR BIOLOGY OF THE CELL

HIV-1 uses dynamic capsid pores to import nucleotides and fuel encapsidated DNA synthesis

(2016) David A. Jacques et al.   NATURE

Analysis of the mechanical properties of wild type and hyperstable mutants of the HIV-1 capsid

(2016) Ruben Ramalho et al.   Retrovirology

The structure and flexibility of conical HIV-1 capsids determined within intact virions

(2016) Simone Mattei et al.   SCIENCE

HIV replication

(2016) Daniel Sauter et al.   Current Opinion in HIV and AIDS

A Novel Entry/Uncoating Assay Reveals the Presence of at Least Two Species of Viral Capsids During Synchronized HIV-1 Infection

(2016) Claire Da Silva Santos et al.   PLoS Pathogens

Time-Resolved Imaging of Single HIV-1 Uncoating In Vitro and in Living Cells

(2016) Ashwanth C. Francis et al.   PLoS Pathogens

HIV-1 capsid: the multifaceted key player in HIV-1 infection

(2015) Edward M. Campbell et al.   NATURE REVIEWS MICROBIOLOGY

Fluorescent protein-tagged Vpr dissociates from HIV-1 core after viral fusion and rapidly enters the cell nucleus

(2015) Tanay M. Desai et al.   Retrovirology

X-ray crystal structures of native HIV-1 capsid protein reveal conformational variability

(2015) A. T. Gres et al.   SCIENCE

FAITH – Fast Assembly Inhibitor Test for HIV

(2015) Romana Hadravová et al.   VIROLOGY

Interactions between HIV-1 and the Cell-Autonomous Innate Immune System

(2014) Greg J. Towers et al.   Cell Host & Microbe

A new probe for super-resolution imaging of membranes elucidates trafficking pathways

(2014) Natalia H. Revelo et al.   JOURNAL OF CELL BIOLOGY

Extreme Genetic Fragility of the HIV-1 Capsid

(2013) Suzannah J. Rihn et al.   PLoS Pathogens

Superresolution imaging of HIV in infected cells with FlAsH-PALM

(2012) M. Lelek et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Labeling of Multiple HIV-1 Proteins with the Biarsenical-Tetracysteine System

(2011) Cândida F. Pereira et al.   PLoS One

Complementary assays reveal a relationship between HIV-1 uncoating and reverse transcription

(2011) A. E. Hulme et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vitro Uncoating of HIV-1 Cores

(2011) Vaibhav B. Shah et al.   Jove-Journal of Visualized Experiments

HIV-1 Pre-Integration Complexes Selectively Target Decondensed Chromatin in the Nuclear Periphery

(2008) Alberto Albanese et al.   PLoS One