Colorectal Cancer Stem Cells: An Overview of Evolving Methods and Concepts

Functional States in Tumor-Initiating Cell Differentiation in Human Colorectal Cancer

(2021) Martina K. Zowada et al.   Cancers

The frontier of live tissue imaging across space and time

(2021) Qiang Huang et al.   Cell Stem Cell

Normal tissue architecture determines the evolutionary course of cancer

(2021) Jeffrey West et al.   Nature Communications

Tracing oncogene-driven remodelling of the intestinal stem cell niche

(2021) Min Kyu Yum et al.   NATURE

NOTUM from Apc-mutant cells biases clonal competition to initiate cancer

(2021) Dustin J. Flanagan et al.   NATURE

Apc-mutant cells act as supercompetitors in intestinal tumour initiation

(2021) Sanne M. van Neerven et al.   NATURE

Adipose stem cell niche reprograms the colorectal cancer stem cell metastatic machinery

(2021) Simone Di Franco et al.   Nature Communications

Recent trends in stem cell-based therapies and applications of artificial intelligence in regenerative medicine

(2021) Sayali Mukherjee et al.   World Journal of Stem Cells

A pre-existing population of ZEB2+ quiescent cells with stemness and mesenchymal features dictate chemoresistance in colorectal cancer

(2020) Federica Francescangeli et al.   JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH

Organoid models of gastrointestinal cancers in basic and translational research

(2020) Harry Cheuk Hay Lau et al.   Nature Reviews Gastroenterology & Hepatology

Epigenetics of colorectal cancer: biomarker and therapeutic potential

(2020) Gerhard Jung et al.   Nature Reviews Gastroenterology & Hepatology

Plasticity of Lgr5-Negative Cancer Cells Drives Metastasis in Colorectal Cancer

(2020) Arianna Fumagalli et al.   Cell Stem Cell

Tools and Concepts for Interrogating and Defining Cellular Identity

(2020) Kara L. McKinley et al.   Cell Stem Cell

Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer

(2020) Clara Morral et al.   Cell Stem Cell

Medium-throughput drug screening of patient-derived organoids from colorectal peritoneal metastases to direct personalized therapy

(2020) Vignesh Narasimhan et al.   CLINICAL CANCER RESEARCH

Paracrine orchestration of intestinal tumorigenesis by a mesenchymal niche

(2020) Manolis Roulis et al.   NATURE

Lymph node metastases develop through a wider evolutionary bottleneck than distant metastases

(2020) Johannes G. Reiter et al.   NATURE GENETICS

Lineage tracing meets single-cell omics: opportunities and challenges

(2020) Daniel E. Wagner et al.   NATURE REVIEWS GENETICS

Cell-by-Cell Deconstruction of Stem Cell Niches

(2020) Anastasia N. Tikhonova et al.   Cell Stem Cell

Tumour budding in solid cancers

(2020) Alessandro Lugli et al.   Nature Reviews Clinical Oncology

Integrating genetic and non-genetic determinants of cancer evolution by single-cell multi-omics

(2020) Anna S. Nam et al.   NATURE REVIEWS GENETICS

Humanized Rodent Models for Cancer Research

(2020) Huimin Tian et al.   Frontiers in Oncology

A tissue engineering approach to metastatic colon cancer

(2020) Samaneh Kamali Sarvestani et al.   iScience

Next-Generation Lineage Tracing and Fate Mapping to Interrogate Development

(2020) Sadie VanHorn et al.   DEVELOPMENTAL CELL

The epigenetic regulator Mll1 is required for Wnt-driven intestinal tumorigenesis and cancer stemness

(2020) Johanna Grinat et al.   Nature Communications

The Balance of Stromal BMP Signaling Mediated by GREM1 and ISLR Drives Colorectal Carcinogenesis

(2020) Hiroki Kobayashi et al.   GASTROENTEROLOGY

Spatiotemporal regulation of clonogenicity in colorectal cancer xenografts

(2019) Maartje van der Heijden et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stem Cell Plasticity and Dormancy in the Development of Cancer Therapy Resistance

(2019) Maria Laura De Angelis et al.   Frontiers in Oncology

Human Organoids: Tools for Understanding Biology and Treating Diseases

(2019) Frans Schutgens et al.   Annual Review of Pathology-Mechanisms of Disease

Patient-Derived Xenografts and Matched Cell Lines Identify Pharmacogenomic Vulnerabilities in Colorectal Cancer

(2019) Luca Lazzari et al.   CLINICAL CANCER RESEARCH

Metastatic seeding of human colon cancer cell clusters expressing the hybrid epithelial/mesenchymal state

(2019) Kosuke Mizukoshi et al.   INTERNATIONAL JOURNAL OF CANCER

A marker-independent lineage-tracing system to quantify clonal dynamics and stem cell functionality in cancer tissue

(2019) Kristiaan J. Lenos et al.   Nature Protocols

Patient-Derived Organoids Predict Chemoradiation Responses of Locally Advanced Rectal Cancer

(2019) Ye Yao et al.   Cell Stem Cell

A rectal cancer organoid platform to study individual responses to chemoradiation

(2019) Karuna Ganesh et al.   NATURE MEDICINE

Preclinical models for precision oncology

(2018) Maider Ibarrola-Villava et al.   BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER

In Silico Labeling: Predicting Fluorescent Labels in Unlabeled Images

(2018) Eric M. Christiansen et al.   CELL

Single-Cell Transcriptomics Meets Lineage Tracing

(2018) Lennart Kester et al.   Cell Stem Cell

Evolutionary history of human colitis-associated colorectal cancer

(2018) Ann-Marie Baker et al.   GUT

Spatial mutation patterns as markers of early colorectal tumor cell mobility

(2018) Marc D. Ryser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ghost cytometry

(2018) Sadao Ota et al.   SCIENCE

Patient-derived organoids model treatment response of metastatic gastrointestinal cancers

(2018) Georgios Vlachogiannis et al.   SCIENCE

A temporal shift of the evolutionary principle shaping intratumor heterogeneity in colorectal cancer

(2018) Tomoko Saito et al.   Nature Communications

Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids

(2018) Krijn K. Dijkstra et al.   CELL

TET2 controls chemoresistant slow-cycling cancer cell survival and tumor recurrence

(2018) Isabel Puig et al.   JOURNAL OF CLINICAL INVESTIGATION

Intra-tumour diversification in colorectal cancer at the single-cell level

(2018) Sophie F. Roerink et al.   NATURE

Stem cell functionality is microenvironmentally defined during tumour expansion and therapy response in colon cancer

(2018) Kristiaan J. Lenos et al.   NATURE CELL BIOLOGY

High-throughput screening in colorectal cancer tissue-originated spheroids

(2018) Jumpei Kondo et al.   CANCER SCIENCE

Organoid Modeling of the Tumor Immune Microenvironment

(2018) James T. Neal et al.   CELL

Phenotypic Plasticity: Driver of Cancer Initiation, Progression, and Therapy Resistance

(2018) Piyush B. Gupta et al.   Cell Stem Cell

Tumour heterogeneity and metastasis at single-cell resolution

(2018) Devon A. Lawson et al.   NATURE CELL BIOLOGY

The practical implementation of artificial intelligence technologies in medicine

(2018) Jianxing He et al.   NATURE MEDICINE

Single-cell multiomics sequencing and analyses of human colorectal cancer

(2018) Shuhui Bian et al.   SCIENCE

CHK1-targeted therapy to deplete DNA replication-stressed, p53-deficient, hyperdiploid colorectal cancer stem cells

(2017) Gwenola Manic et al.   GUT

Genetic subclone architecture of tumor clone-initiating cells in colorectal cancer

(2017) Klara M. Giessler et al.   JOURNAL OF EXPERIMENTAL MEDICINE

A distinct role for Lgr5+ stem cells in primary and metastatic colon cancer

(2017) Felipe de Sousa e Melo et al.   NATURE

Visualization and targeting of LGR5+ human colon cancer stem cells

(2017) Mariko Shimokawa et al.   NATURE

Transplantation of engineered organoids enables rapid generation of metastatic mouse models of colorectal cancer

(2017) Kevin P O'Rourke et al.   NATURE BIOTECHNOLOGY

Reference component analysis of single-cell transcriptomes elucidates cellular heterogeneity in human colorectal tumors

(2017) Huipeng Li et al.   NATURE GENETICS

Cancer stem cells revisited

(2017) Eduard Batlle et al.   NATURE MEDICINE

Genetic dissection of colorectal cancer progression by orthotopic transplantation of engineered cancer organoids

(2017) Arianna Fumagalli et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Origins of lymphatic and distant metastases in human colorectal cancer

(2017) Kamila Naxerova et al.   SCIENCE

Multicolor lineage tracing reveals clonal architecture and dynamics in colon cancer

(2017) Sebastian Lamprecht et al.   Nature Communications

Engineering Stem Cell Organoids

(2016) Xiaolei Yin et al.   Cell Stem Cell

A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements during Tumorigenesis

(2016) Masayuki Fujii et al.   Cell Stem Cell

Circulating tumour cells from patients with colorectal cancer have cancer stem cell hallmarks inex vivoculture

(2016) Fanny Grillet et al.   GUT

ARID1A loss impairs enhancer-mediated gene regulation and drives colon cancer in mice

(2016) Radhika Mathur et al.   NATURE GENETICS

Cancer Stem Cell-Based Models of Colorectal Cancer Reveal Molecular Determinants of Therapy Resistance

(2016) Maria Laura De Angelis et al.   Stem Cells Translational Medicine

Dynamic regulation of the cancer stem cell compartment by Cripto-1 in colorectal cancer

(2015) F Francescangeli et al.   CELL DEATH AND DIFFERENTIATION

Krt19+/Lgr5− Cells Are Radioresistant Cancer-Initiating Stem Cells in the Colon and Intestine

(2015) Samuel Asfaha et al.   Cell Stem Cell

The rationale for liquid biopsy in colorectal cancer: a focus on circulating tumor cells

(2015) Paola Gazzaniga et al.   EXPERT REVIEW OF MOLECULAR DIAGNOSTICS

Sequential cancer mutations in cultured human intestinal stem cells

(2015) Jarno Drost et al.   NATURE

A Big Bang model of human colorectal tumor growth

(2015) Andrea Sottoriva et al.   NATURE GENETICS

High-throughput screening using patient-derived tumor xenografts to predict clinical trial drug response

(2015) Hui Gao et al.   NATURE MEDICINE

Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

(2015) Mami Matano et al.   NATURE MEDICINE

CD44v6 Is a Marker of Constitutive and Reprogrammed Cancer Stem Cells Driving Colon Cancer Metastasis

(2014) Matilde Todaro et al.   Cell Stem Cell

Colorectal Cancer Stem Cells: From the Crypt to the Clinic

(2014) Ann Zeuner et al.   Cell Stem Cell

Lgr5+ Stem Cells Are Indispensable for Radiation-Induced Intestinal Regeneration

(2013) Ciara Metcalfe et al.   Cell Stem Cell

The epigenetics of epithelial-mesenchymal plasticity in cancer

(2013) Wai Leong Tam et al.   NATURE MEDICINE

Lineage tracing reveals multipotent stem cells maintain human adenomas and the pattern of clonal expansion in tumor evolution

(2013) A. Humphries et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Circulating tumour cells escape from EpCAM-based detection due to epithelial-to-mesenchymal transition

(2012) Tobias M Gorges et al.   BMC CANCER

Dependency of Colorectal Cancer on a TGF-β-Driven Program in Stromal Cells for Metastasis Initiation

(2012) Alexandre Calon et al.   CANCER CELL

Lineage Tracing

(2012) Kai Kretzschmar et al.   CELL

Intestinal Tumorigenesis Initiated by Dedifferentiation and Acquisition of Stem-Cell-like Properties

(2012) Sarah Schwitalla et al.   CELL

Mutations in the Ras-Raf Axis Underlie the Prognostic Value of CD133 in Colorectal Cancer

(2012) K. Kemper et al.   CLINICAL CANCER RESEARCH

Inhibition of MEK and PI3K/mTOR Suppresses Tumor Growth but Does Not Cause Tumor Regression in Patient-Derived Xenografts of RAS-Mutant Colorectal Carcinomas

(2012) G. Migliardi et al.   CLINICAL CANCER RESEARCH

Dll1+ secretory progenitor cells revert to stem cells upon crypt damage

(2012) Johan H. van Es et al.   NATURE CELL BIOLOGY

Variable Clonal Repopulation Dynamics Influence Chemotherapy Response in Colorectal Cancer

(2012) A. Kreso et al.   SCIENCE

Lineage Tracing Reveals Lgr5+ Stem Cell Activity in Mouse Intestinal Adenomas

(2012) A. G. Schepers et al.   SCIENCE

Proliferation State and Polo-Like Kinase1 Dependence of Tumorigenic Colon Cancer Cells

(2012) Federica Francescangeli et al.   STEM CELLS

Distinct Types of Tumor-Initiating Cells Form Human Colon Cancer Tumors and Metastases

(2011) Sebastian M. Dieter et al.   Cell Stem Cell

The Intestinal Stem Cell Signature Identifies Colorectal Cancer Stem Cells and Predicts Disease Relapse

(2011) Anna Merlos-Suárez et al.   Cell Stem Cell

Long-term Expansion of Epithelial Organoids From Human Colon, Adenoma, Adenocarcinoma, and Barrett's Epithelium

(2011) Toshiro Sato et al.   GASTROENTEROLOGY

CD133 ProteinN-Glycosylation Processing Contributes to Cell Surface Recognition of the Primitive Cell Marker AC133 Epitope

(2011) Anthony B. Mak et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Single-cell dissection of transcriptional heterogeneity in human colon tumors

(2011) Piero Dalerba et al.   NATURE BIOTECHNOLOGY

Retaining cell-cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer

(2011) J. Kondo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Molecularly Annotated Platform of Patient-Derived Xenografts ("Xenopatients") Identifies HER2 as an Effective Therapeutic Target in Cetuximab-Resistant Colorectal Cancer

(2011) A. Bertotti et al.   Cancer Discovery

Prognostic impact of the expression of putative cancer stem cell markers CD133, CD166, CD44s, EpCAM and ALDH1 in colorectal cancer

(2010) A Lugli et al.   BRITISH JOURNAL OF CANCER

The AC133 Epitope, but not the CD133 Protein, Is Lost upon Cancer Stem Cell Differentiation

(2010) K. Kemper et al.   CANCER RESEARCH

New models for cancer research: human cancer stem cell xenografts

(2010) Marta Baiocchi et al.   CURRENT OPINION IN PHARMACOLOGY

Bone Morphogenetic Protein 4 Induces Differentiation of Colorectal Cancer Stem Cells and Increases Their Response to Chemotherapy in Mice

(2010) Ylenia Lombardo et al.   GASTROENTEROLOGY

Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts

(2010) Toshiro Sato et al.   NATURE

Wnt activity defines colon cancer stem cells and is regulated by the microenvironment

(2010) Louis Vermeulen et al.   NATURE CELL BIOLOGY

ABCG5-positivity in tumor buds is an indicator of poor prognosis in node-negative colorectal cancer patients

(2010) lsabel Hostettler   WORLD JOURNAL OF GASTROENTEROLOGY

Aldehyde Dehydrogenase 1 Is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation during Colon Tumorigenesis

(2009) E. H. Huang et al.   CANCER RESEARCH

ELDA: Extreme limiting dilution analysis for comparing depleted and enriched populations in stem cell and other assays

(2009) Yifang Hu et al.   JOURNAL OF IMMUNOLOGICAL METHODS

The cancer stem cell marker CD133 has high prognostic impact but unknown functional relevance for the metastasis of human colon cancer

(2009) David Horst et al.   JOURNAL OF PATHOLOGY

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

DACT3 Is an Epigenetic Regulator of Wnt/β-Catenin Signaling in Colorectal Cancer and Is a Therapeutic Target of Histone Modifications

(2008) Xia Jiang et al.   CANCER CELL

CD133 expression is not restricted to stem cells, and both CD133+ and CD133– metastatic colon cancer cells initiate tumors

(2008) Sergey V. Shmelkov et al.   JOURNAL OF CLINICAL INVESTIGATION

Prominin 1 marks intestinal stem cells that are susceptible to neoplastic transformation

(2008) Liqin Zhu et al.   NATURE

Crypt stem cells as the cells-of-origin of intestinal cancer

(2008) Nick Barker et al.   NATURE

Efficient tumour formation by single human melanoma cells

(2008) Elsa Quintana et al.   NATURE

Single-cell cloning of colon cancer stem cells reveals a multi-lineage differentiation capacity

(2008) L. Vermeulen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA