One-step synthesis of site-specific antibody–drug conjugates by reprograming IgG glycoengineering with LacNAc-based substrates

Recent progress in transglutaminase-mediated assembly of antibody-drug conjugates

(2020) Hendrik Schneider et al.   ANALYTICAL BIOCHEMISTRY

Glycoengineering of Antibodies for Modulating Functions

(2019) Lai-Xi Wang et al.   Annual Review of Biochemistry

Rapid and Efficient Generation of Stable Antibody-Drug Conjugates via an Encoded Cyclopropene and an Inverse-Electron-Demand Diels-Alder Reaction

(2018) Benjamí Oller-Salvia et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

One-Pot Dual Labeling of IgG 1 and Preparation of C-to-C Fusion Proteins Through a Combination of Sortase A and Butelase 1

(2018) Thibault J. Harmand et al.   BIOCONJUGATE CHEMISTRY

Chemoenzymatic synthesis of glycoengineered IgG antibodies and glycosite-specific antibody–drug conjugates

(2017) Feng Tang et al.   Nature Protocols

Strategies and challenges for the next generation of antibody–drug conjugates

(2017) Alain Beck et al.   NATURE REVIEWS DRUG DISCOVERY

Modulating IgG effector function by Fc glycan engineering

(2017) Tiezheng Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Optimal Synthetic Glycosylation of a Therapeutic Antibody

(2016) Thomas B. Parsons et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Glycosynthase Mutants of Endoglycosidase S2 Show Potent Transglycosylation Activity and Remarkably Relaxed Substrate Specificity for Antibody Glycosylation Remodeling

(2016) Tiezheng Li et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Endo-F3 Glycosynthase Mutants Enable Chemoenzymatic Synthesis of Core-fucosylated Triantennary Complex Type Glycopeptides and Glycoproteins

(2016) John P. Giddens et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

One-pot N-glycosylation remodeling of IgG with non-natural sialylglycopeptides enables glycosite-specific and dual-payload antibody–drug conjugates

(2016) Feng Tang et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Straightforward Glycoengineering Approach to Site-Specific Antibody–Pyrrolobenzodiazepine Conjugates

(2016) Pamela Thompson et al.   ACS Medicinal Chemistry Letters

Chemoenzymatic Conjugation of Toxic Payloads to the Globally Conserved N-Glycan of Native mAbs Provides Homogeneous and Highly Efficacious Antibody–Drug Conjugates

(2015) Remon van Geel et al.   BIOCONJUGATE CHEMISTRY

EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans

(2015) Jonathan Sjögren et al.   GLYCOBIOLOGY

Site-Specific Conjugation of Monomethyl Auristatin E to Anti-CD30 Antibodies Improves Their Pharmacokinetics and Therapeutic Index in Rodent Models

(2015) F. Lhospice et al.   MOLECULAR PHARMACEUTICS

Sortase Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody Drug Conjugates with High In Vitro and In Vivo Potency

(2015) Roger R. Beerli et al.   PLoS One

Preparation of Well-Defined Antibody-Drug Conjugates through Glycan Remodeling and Strain-Promoted Azide-Alkyne Cycloadditions

(2014) Xiuru Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

(2014) Erik S. Zimmerman et al.   BIOCONJUGATE CHEMISTRY

Site-Specific Antibody–Drug Conjugation through Glycoengineering

(2014) Qun Zhou et al.   BIOCONJUGATE CHEMISTRY

A simplified procedure for gram-scale production of sialylglycopeptide (SGP) from egg yolks and subsequent semi-synthesis of Man3GlcNAc oxazoline

(2014) Bingyang Sun et al.   CARBOHYDRATE RESEARCH

Site-specific antibody-drug conjugation through an engineered glycotransferase and a chemically reactive sugar

(2014) Zhongyu Zhu et al.   mAbs

A Practical One-Step Synthesis of 1,2-Oxazoline Derivatives from Unprotected Sugars and Its Application to Chemoenzymaticβ-N-Acetylglucosaminidation of Disialo-oligosaccharide

(2012) Masato Noguchi et al.   HELVETICA CHIMICA ACTA

Remarkable Transglycosylation Activity of Glycosynthase Mutants of Endo-D, an Endo-β-N-acetylglucosaminidase fromStreptococcus pneumoniae

(2012) Shu-Quan Fan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

An Endoglycosidase with Alternative Glycan Specificity Allows Broadened Glycoprotein Remodelling

(2012) Jonathan J. Goodfellow et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemoenzymatic Glycoengineering of Intact IgG Antibodies for Gain of Functions

(2012) Wei Huang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids

(2012) J. Y. Axup et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Site-Specific and Stoichiometric Modification of Antibodies by Bacterial Transglutaminase

(2010) Simone Jeger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Arthrobacter Endo-β-N-Acetylglucosaminidase Shows Transglycosylation Activity on Complex-Type N-Glycan Oxazolines: One-Pot Conversion of Ribonuclease B to Sialylated Ribonuclease C

(2010) Wei Huang et al.   CHEMBIOCHEM

Enzymatic transglycosylation for glycoconjugate synthesis

(2009) Lai-Xi Wang et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Glycosynthases Enable a Highly Efficient Chemoenzymatic Synthesis ofN-Glycoproteins Carrying Intact NaturalN-Glycans

(2009) Wei Huang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index

(2008) Jagath R Junutula et al.   NATURE BIOTECHNOLOGY