Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance

Translational Control by Ribosome Pausing in Bacteria: How a Non-uniform Pace of Translation Affects Protein Production and Folding

(2021) Ekaterina Samatova et al.   Frontiers in Microbiology

Rapid Increase in the IS26-Mediated cfr Gene in E. coli Isolates with IncP and IncX4 Plasmids and Co-Existing cfr and mcr-1 Genes in a Swine Farm

(2021) Zhenbao Ma et al.   Pathogens

Applications of Bacterial Degrons and Degraders — Toward Targeted Protein Degradation in Bacteria

(2021) Matylda Anna Izert et al.   Frontiers in Molecular Biosciences

Mobile Oxazolidinone Resistance Genes in Gram-Positive and Gram-Negative Bacteria

(2021) Stefan Schwarz et al.   CLINICAL MICROBIOLOGY REVIEWS

Mechanisms of Cotranslational Protein Maturation in Bacteria

(2021) Jiří Koubek et al.   Frontiers in Molecular Biosciences

Assessment of the nucleotide modifications in the high-resolution cryo-electron microscopy structure of the Escherichia coli 50S subunit

(2020) Vanja Stojković et al.   NUCLEIC ACIDS RESEARCH

Translational initiation in E. coli occurs at the correct sites genome-wide in the absence of mRNA-rRNA base-pairing

(2020) Kazuki Saito et al.   eLife

Domains two and three of Escherichia coli ribosomal S1 protein confers 30S subunits a high affinity for downstream A/U-rich mRNAs

(2019) Juan C Cifuentes-Goches et al.   JOURNAL OF BIOCHEMISTRY

cfr(B), cfr(C), and a New cfr-Like Gene, cfr(E), in Clostridium difficile Strains Recovered across Latin America

(2019) Vanja Stojković et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Linezolid-resistant ST872 Enteroccocus faecium harbouring optrA and cfr (D) oxazolidinone resistance genes

(2019) Stanley Pang et al.   INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS

A short translational ramp determines the efficiency of protein synthesis

(2019) Manasvi Verma et al.   Nature Communications

How Messenger RNA and Nascent Chain Sequences Regulate Translation Elongation

(2018) Junhong Choi et al.   Annual Review of Biochemistry

Investigation of Solvent Hydron Exchange in the Reaction Catalyzed by the Antibiotic Resistance Protein, Cfr

(2018) Matthew R. Bauerle et al.   BIOCHEMISTRY

Emergence and control of linezolid-resistant Staphylococcus epidermidis in an ICU of a German hospital

(2018) Christina Weßels et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Dissemination of linezolid-dependent, linezolid-resistant Staphylococcus epidermidis clinical isolates belonging to CC5 in German hospitals

(2018) Franziska Layer et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Total Synthesis of (+)-Pleuromutilin

(2018) Elliot P. Farney et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Accessibility of the Shine-Dalgarno Sequence Dictates N-Terminal Codon Bias in E. coli

(2018) Sanchari Bhattacharyya et al.   MOLECULAR CELL

Antibacterial properties and clinical potential of pleuromutilins

(2018) Olivia Goethe et al.   NATURAL PRODUCT REPORTS

The cfr and cfr-like multiple resistance genes

(2018) Birte Vester   RESEARCH IN MICROBIOLOGY

cisTEM, user-friendly software for single-particle image processing

(2018) Timothy Grant et al.   eLife

Long-lasting successful dissemination of resistance to oxazolidinones in MDR Staphylococcus epidermidis clinical isolates in a tertiary care hospital in France

(2017) Laurent Dortet et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Novel multiresistance cfr plasmids in linezolid-resistant methicillin-resistant Staphylococcus epidermidis and vancomycin-resistant Enterococcus faecium (VRE) from a hospital outbreak: co-location of cfr and optrA in VRE

(2017) Alexandros Lazaris et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

A modular and enantioselective synthesis of the pleuromutilin antibiotics

(2017) Stephen K. Murphy et al.   SCIENCE

Distribution of cfr in Staphylococcus spp. and Escherichia coli Strains from Pig Farms in China and Characterization of a Novel cfr-Carrying F43:A-:B- Plasmid

(2017) Xiao-Qin Liu et al.   Frontiers in Microbiology

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

Blast from the Past: Reassessing Forgotten Translation Inhibitors, Antibiotic Selectivity, and Resistance Mechanisms to Aid Drug Development

(2016) Stefan Arenz et al.   MOLECULAR CELL

Codon influence on protein expression in E. coli correlates with mRNA levels

(2016) Grégory Boël et al.   NATURE

Structure of the 70S ribosome from human pathogen Staphylococcus aureus

(2016) Iskander Khusainov et al.   NUCLEIC ACIDS RESEARCH

Antibiotic resistance evolved via inactivation of a ribosomal RNA methylating enzyme

(2016) Vanja Stojković et al.   NUCLEIC ACIDS RESEARCH

The ribosome in action: Tuning of translational efficiency and protein folding

(2016) Marina V. Rodnina   PROTEIN SCIENCE

Crystallographic capture of a radical S-adenosylmethionine enzyme in the act of modifying tRNA

(2016) E. L. Schwalm et al.   SCIENCE

Lincosamides, Streptogramins, Phenicols, and Pleuromutilins: Mode of Action and Mechanisms of Resistance

(2016) Stefan Schwarz et al.   Cold Spring Harbor Perspectives in Medicine

Detection of a Newcfr-Like Gene,cfr(B), in Enterococcus faecium Isolates Recovered from Human Specimens in the United States as Part of the SENTRY Antimicrobial Surveillance Program

(2015) Lalitagauri M. Deshpande et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Acfr-Like Gene from Clostridium difficile Confers Multiple Antibiotic Resistance by the Same Mechanism as thecfrGene

(2015) Lykke H. Hansen et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Dissemination of the Samecfr-Carrying Plasmid among Methicillin-Resistant Staphylococcus aureus and Coagulase-Negative Staphylococcal Isolates in China

(2015) Jia Chang Cai et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Distinct tRNA Accommodation Intermediates Observed on the Ribosome with the Antibiotics Hygromycin A and A201A

(2015) Yury S. Polikanov et al.   MOLECULAR CELL

Structure of the human 80S ribosome

(2015) Heena Khatter et al.   NATURE

I-TASSER server: new development for protein structure and function predictions

(2015) Jianyi Yang et al.   NUCLEIC ACIDS RESEARCH

Clostridium difficile Isolates with High Linezolid MICs Harbor the Multiresistance Genecfr

(2014) Mercedes Marín et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Quantifying Absolute Protein Synthesis Rates Reveals Principles Underlying Allocation of Cellular Resources

(2014) Gene-Wei Li et al.   CELL

Identification of the Multi-Resistance Gene cfr in Escherichia coli Isolates of Animal Origin

(2014) Hui Deng et al.   PLoS One

Presence and dissemination of the multiresistance gene cfr in Gram-positive and Gram-negative bacteria

(2013) J. Shen et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Efficient translation initiation dictates codon usage at gene start

(2013) K. Bentele et al.   Molecular Systems Biology

Ribosome-targeting antibiotics and mechanisms of bacterial resistance

(2013) Daniel N. Wilson   NATURE REVIEWS MICROBIOLOGY

Causes and Effects of N-Terminal Codon Bias in Bacterial Genes

(2013) D. B. Goodman et al.   SCIENCE

Mechanisms of Protein Sequence Divergence and Incompatibility

(2013) Alon Wellner et al.   PLoS Genetics

The Genetic Environment of thecfrGene and the Presence of Other Mechanisms Account for the Very High Linezolid Resistance of Staphylococcus epidermidis Isolate 426-3147L

(2012) Jacqueline LaMarre et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Detection of the staphylococcal multiresistance gene cfr in Escherichia coli of domestic-animal origin

(2012) Y. Wang et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Covalent Intermediate in the Catalytic Mechanism of the Radical S-Adenosyl-l-methionine Methyl Synthase RlmN Trapped by Mutagenesis

(2012) Kevin P. McCusker et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

ViennaRNA Package 2.0

(2011) Ronny Lorenz et al.   Algorithms for Molecular Biology

Low Fitness Cost of the Multidrug Resistance Genecfr

(2011) Jacqueline M. LaMarre et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

The N-end rule pathway: From recognition by N-recognins, to destruction by AAA+proteases

(2011) D.A. Dougan et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR

(2011) Kang Zhou et al.   BMC MOLECULAR BIOLOGY

RNA methylation by Radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift

(2011) F. Yan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Radically Different Mechanism for S-Adenosylmethionine-Dependent Methyltransferases

(2011) T. L. Grove et al.   SCIENCE

Structural Basis for Methyl Transfer by a Radical SAM Enzyme

(2011) A. K. Boal et al.   SCIENCE

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Elevated Linezolid Resistance in Clinical cfr-Positive Staphylococcus aureus Isolates Is Associated with Co-Occurring Mutations in Ribosomal Protein L3

(2010) J. B. Locke et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Protein N-Terminal Processing: Substrate Specificity ofEscherichia coliand Human Methionine Aminopeptidases

(2010) Qing Xiao et al.   BIOCHEMISTRY

An Evolutionarily Conserved Mechanism for Controlling the Efficiency of Protein Translation

(2010) Tamir Tuller et al.   CELL

Resistance to Linezolid Is Mediated by thecfrGene in the First Report of an Outbreak of Linezolid‐ResistantStaphylococcus aureus

(2010) Gracia Morales et al.   CLINICAL INFECTIOUS DISEASES

Multicity Outbreak of Linezolid‐ResistantStaphylococcus epidermidisAssociated with Clonal Spread of acfr‐Containing Strain

(2010) Hector Bonilla et al.   CLINICAL INFECTIOUS DISEASES

RlmN and Cfr are Radical SAM Enzymes Involved in Methylation of Ribosomal RNA

(2010) Feng Yan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

electronic Ligand Builder and Optimization Workbench(eLBOW): a tool for ligand coordinate and restraint generation

(2009) Nigel W. Moriarty et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

The A–Z of bacterial translation inhibitors

(2009) Daniel N. Wilson   CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY

YgdE is the 2′-O-ribose methyltransferase RlmM specific for nucleotide C2498 in bacterial 23S rRNA

(2009) Elzbieta Purta et al.   MOLECULAR MICROBIOLOGY

Insights into the structure, function and evolution of the radical-SAM 23S rRNA methyltransferase Cfr that confers antibiotic resistance in bacteria

(2009) Katarzyna H. Kaminska et al.   NUCLEIC ACIDS RESEARCH

Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria

(2009) A. M. B. Giessing et al.   RNA

Coding-Sequence Determinants of Gene Expression in Escherichia coli

(2009) G. Kudla et al.   SCIENCE

Linezolid Resistance in Staphylococcus aureus: Gene Dosage Effect, Stability, Fitness Costs, and Cross-Resistances

(2008) S. Besier et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Transcriptional and Translational Control of the mlr Operon, Which Confers Resistance to Seven Classes of Protein Synthesis Inhibitors

(2008) L. K. Smith et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Clinical and Microbiological Aspects of Linezolid Resistance Mediated by the cfr Gene Encoding a 23S rRNA Methyltransferase

(2008) C. A. Arias et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances

(2008) Irith Wiegand et al.   Nature Protocols